接合桿 的英文怎麼說
中文拼音 [jiēgěgǎn]
接合桿
英文
engaging lever-
Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method
利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。With the help of the electronic computers, engineers employ it to figure out nearly all kinds of engineering problems ranging from astronavigation, construction, waterpower projects, ship - building and mechanical engineering etc. owing to the strong power of finite element method, one kind of software pack which based on the method is exploited and named algor feas to resolve problems on structure analysis for trusses and shells. this paper also employ algor feas software pack to analyze and compute the cableway towers, giving the accurate result on stress and displacement both in location and whole. finally, we also bring out some advice on structure improving
在此分析過程中：首先將物理模型簡化為力學模型，主要是將塔架結構按空間桿單元簡化為空間桁架結構，目的是將力學模型變換為數學模型；這一過程主要是利用autocad2000建立塔架的計算模型；再者，利用algorfeas軟體包讀入autocad建立的塔架計算模型生成初始數據卡利用計算機進行編程進行風載計算，再將計算結論整合入algorfeas產生的初始數據卡中，生成計算數據卡；接著，利用algorfeas軟體包計算塔架在不同載荷情況下的應力圖，位移圖，並且求出在不同載荷條件下的最大位移和最大應力等；然後，對塔架的穩定性進行分析；最後；根據計算結果提出改進建議。This study adopted the ion compound antibacterial to produce the materials of antibacterial glass. two kinds of different carriers are used in this experiment, phosphate and borate system. the antibacterial glass material, which is added ag +, zn2 + through some carriers, has excellent antibacterial property against escherichia coli and staphylococcus aurous
實驗中採用兩種不同的玻璃載體體系，即磷酸鹽載體和硼硅酸鹽載體，將銀、鋅離子以一定的方式直接加入到玻璃生產的配合料中，一次性熔製成形，能夠制備出對大腸桿菌、金黃色葡萄球菌等細菌具有良好抗菌效果的抗菌玻璃材料。Not to inherit by right of primogeniture, gavelkind or borough english, or possess in perpetuity an extensive demesne of a sufficient number of acres, roods and perches, statute land measure valuation 42, of grazing turbary surrounding a baronial hall with gatelodge and carriage drive nor, on the other hand, a terracehouse or semidetached villa, described as rus in urbe or qui si sana, but to purchase by private treaty in fee simple a thatched bungalowshaped 2 storey dwellinghouse of southerly aspect, surmounted by vane and lightning conductor, connected with the earth, with porch covered by parasitic plants ivy or virginia creeper, halldoor, olive green, with smart carriage finish and neat doorbrasses, stucco front with gilt tracery at eaves and gable, rising, if possible, upon a gentle eminence with agreeable prospect from balcony with stone pillar parapet over unoccupied and unoccupyable interjacent pastures and standing in 5 or 6 acres of its own ground, at such a distance from the nearest public thoroughfare as to render its houselights visible at night above and through a quickset hornbeam hedge of topiary cutting, situate at a given point not less than 1 statute mile from the periphery of the metropolis, within a time limit of not more than 5 minutes from tram or train line e. g.,
他並不想根據長子繼承製男子平分繼承製或末子繼承製237 ，把那幢有著門房和馬車道的男爵宅邪及其周圍那一大片遼闊的英畝路得和平方桿238法定土地面積單位，估價為四十二英鎊239的泥炭質牧場地，或者那座被描述為「都會中的田園240 」或「健康莊242 」的有陽臺的房子或一側與鄰屋相接的別墅，繼承下來並永久佔有。他只巴望根據私人合同購買一所繼承人身分不受限制的不動產：要坐北朝南的一座草屋頂有涼臺的雙層住宅，房頂上裝起風向標以及與地面相接的避雷針，門廊上要爬滿寄生植物常春藤或五葉地錦，橄欖綠色的正門最後一道工序漆得漂漂亮亮，賽得過馬車。門上有著精巧的黃銅裝飾。In the simulation, the mpc technique of msc. nastran is used to simulate the mechanical behavior of contact between the interfaces of piston pin and bearing of small end, crankpin and big end bearing, connecting rod body and bearing cap ; also constraints are applied to the connecting rod reasonably
分析中採用了patran中的多點約束（ mpc ）技術，有效地模擬了活塞銷與小頭孔內表面、曲柄銷與大頭孔內表面以及連桿體與大頭蓋接觸面的力學接觸狀態，並對連桿進行合理的約束。In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。The brewing characteristics of shaoxing wine were presented in the following eleven aspects : the uniqueness of adjunct materials and the variety of different wine, the treatment of soaking rice at low temperature for a long time, the variety of fermentation state, the characteristics of rice wine broth, the variety and complexity of microorganisms, the uniqueness of inoculation and strain preservation, the process of mixed fermentation of yeast and lactic acid bacteria ( e. g. the concurrence of saccharification, yeast fermentation and lactic fermentation ), the over - mixing of different liquors, the high sterilization temperature, and the storage of produced wine
摘要該文從11個方面論述了紹興黃酒的釀造特點，即配料的特殊性和酒種的多樣性；低溫長時間浸米；發酵狀態的多樣性；黃酒醪的發酵特點；微生物的多樣性，復雜性；接種方式獨特；菌種保存方法獨特；酵母與乳酸桿菌協同作用的混合發酵并行的過程（即邊糖化、邊酵母發酵、邊乳酸發酵同時進行的三邊發酵） ；酒液勾兌；較高的滅菌溫度；成品酒的貯存。The wells of elisa plate were coated with pab ( 100ng / l ) against h3n2, then phage was added to the wells. after incubation, the wells were washed vigorously with tbst to remove nonbinding phage. phage bound to the antibody were eluted with 0. 2mol / l glycine - hcl ( ph2. 2 ) for 10 min at room temperature and neutrialized with 2mol / l tris - hcl ( ph9. 1 )
以抗h3n2流感病毒的多克隆抗體（ 100ng l ）包被酶標板，加入制備好的肽庫，用tbst洗去非特異結合的噬菌體，加0 . 2mol l甘氨酸-鹽酸（ ph2 . 2 ） ，室溫放置10min以洗脫特異結合的噬菌體， 2mol ltris - hcl （ ph9 . 1 ）中和后，取2 l噬菌體接種大腸桿菌xl1 - blue菌進行空斑滴定，其餘噬菌體擴增後用于下一輪篩選，共重復3輪淘洗。Pp / montmori 1loni te nanocompos1tes ( pcn ) were prepared in two steps. in the first step, mother materia1 was synthesi zed by in - - situ gmft - - fnterca1a tion method, and pcn was prepared by b1ending p1ai n pp and mother material on twin screw extruder in the second step
通過原位接枝插層法制備了pp蒙脫土納米復合增強母料，然後將pp與母料在雙螺桿上共混制得pp蒙脫土納米復合材料（ pcn ） 。Webbing sling, round sling, lifting sling, nylon rope, rope ladder, cargo net lifting sling belt group have a long history for more than 46 years of specializing in different types of slings, mainly products including polyester webbing sling, polyester round sling, polyester cargo lashing, super sing, flat sling, flat webbing sling, flat web sling, polester web sling, tree strap, webbing strap, doublebraid, recovery strap, recovery tow strap, lashing systems, polyester sling, polyester belt sling, hollow braid, synthetic webbing, endless sling, nylon sling, synthetic web sling, synthetic sling, webbing sling, round sling, cargo lashing, ratchet tie down, ratchet lashing, ratchet strap, ratchet tie down strap, nylon webbing, nylon strap, tubular round ropes, braided rope, diamond braid rope, starter rope, safety net, safety belt, cargo net, safety rope, climbing rope, rope ladder, building net, nylon net, polyester webbing sling, polyester round sling, tie down, cargo restraint, webbing strap, duplex eye - eye webbing sling, eye - eye webbing sling endless webbing sling, widely and heavy duty webbing sling, multi - sling, ratchet lashing, tow strap, web belt sling, flat webbing sling, nylon rope ladder, industrial safety nets, nylon cargo net, dock net, synthetic webbing sling, lifting clamp, braided rope, 3 strand rope, 12 strand rope, 8 strand rope, cargo net, shipmast net, tube net, solid braided rope, braided rope net, sports net, pe net, safety net, scaffold net, synthetic slings, wide lift sling, round webbing sling, flat webbing sling with eyes, flat webbing endless sling, wire rope sling, multi - set sling, pipe lifting sling, pipeline sling, lowering belt, heavy duty anti cutting sleeves, fiberglass rope, fiberglass wick, braided fiberglass rope, fiberglass rope lamp, fiberglass oil lamp wick, fiberglass strap, solid braided fiberglass rope - round, fiberglass tapes, ceramic fiber rope, fiberglass braided rope, pipe handling sling, lowering in belts, stone handling sling, flat lifting sling pipe lifting belt synthetic fiber round sling synthetic lifting sling, synthetic fiber sling, nylon webbing strap, pipe sling, bandsling, pipe sling with reinforced eyes, lifting sling with reinforced eyes, pipeline pipe industrial farm lifting sling, endless sling, eye eye sling, oil field sling, truck strap, winch strap, cargo tie down, track ratchet strap, tow strap winch flat rope tow line, heavy duty webbing towing lifting sling, cargo sling, pp cargo sling, polyester cargo sling, flat pack sling, ceramic fiber rope twisted rope braided round rope braided square rope
2圓形尼吊裝繩系列：無接頭尼吊裝繩兩頭扣尼吊裝繩多叉尼吊裝繩船用錨繩纜繩引紙繩變壓器吊裝繩各種規格編織吊繩，尼繩，啟動拉繩，啟動繩，引擎拉繩，引擎繩，起動拉繩，起動繩，動力繩，安全清洗繩，安全繩，保險繩，耐火救生繩，維綸繩，丙綸繩，滌綸繩，錦綸繩，尼吊繩，尼吊裝繩，錨繩，靠岸繩，旗繩，晾衣繩，復合吊繩，掛衣繩，宿營繩，保護繩，防火繩，尼網，休閑吊網，跳繩，蠶絲繩，全棉繩，塑膠繩，布繩，拔河繩，攀巖繩，遊艇繩，綿綸絞制繩，織制繩，登山繩，拔河繩，立桿繩，水平繩，工藝繩，吊繩，船用纜繩，絞制纜繩，綿綸絕緣繩，三股繩，八股繩，軍用繩，線路牽引繩，吊棕繩，割刀繩，引紙繩，包裝袋繩，禮品盒繩，酒盒繩，食品盒繩，手提袋用繩，禮品盒用繩，工藝品用繩，服裝用繩，聚乙烯繩，聚丙烯繩，混紡繩，纖維綸繩，復絲繩，撕裂膜繩，編織繩，加鉛繩，功能繩，軟梯繩，尼軟梯，軟梯，尼繩梯，尼梯繩，安全網，棉繩，彈力橡筋繩，嵌邊繩，封蠟繩等，啟動繩啟動拉繩引擎繩引擎拉繩起動拉繩，雙編織繩，金剛打編織繩，股編織繩，股編織繩，多股編織繩。Bpec handrail, a new type compositive handrailing which hollow ball, metal stucture, its stanchion is set up with several metal hollow balls and pipc through welding, the composition at worksite is very simple and easy without any welding, economic & fine outlook, stable, convenient for installing and separaing
博佩克欄桿是一種新型的空心球金屬結構組合式欄桿，立柱由若干個金屬空心球和同一材料的金屬管通過焊接成為柱子；扶手，橫桿與立桿採用現場組裝，可不用焊接，具有經濟美觀，安裝及拆卸簡便，牢固耐用的優點。2. an up - dated method was employed to purify tumv in this research. using the protease k method, we acquired the viral genome - rna. a pair of specific primers was designed and synthesized based on the nucleotide sequences of tumv coat protein genes reported before and rt - pcr was used to clone the cp genes of the six tumv isolates
應用改進的蕪菁花葉病毒的提取方法從病葉中提取病毒粒體，應用蛋白酶k法從病毒粒體中提取病毒rna基因組，根據已報道的tumv的cp基因序列，設計併合成了一對特異引物，利用rt - pcr法克隆了6個分離物的外殼蛋白基因，與克隆載體puc19連接后通過熱激法轉化大腸桿菌dh5 。Pin insulation cement
接線桿絕緣物膠合物Due to the toxin ' s pharmacological effect of inhibiting acetylcholine release from presynaptic neuromuscular junctions, botulinum a toxin has been used for the treatment of spastic urethral sphincter as well as detrusor overactivity associated with both neurogenic and non - neurogenic etiologies
基於它在藥理學上可以抑制神經肌肉接合前之乙醯膽鹼之釋放，肉毒桿菌毒素也被應用於神經性因或非神經性因之尿道外括約肌痙攣及逼尿肌活性過強。Extensions for tools with driving square according to din 10
符合din 10的方頭傳動刀具用接長桿Based on a 3. 1kb pst i fragment of genomic dna of a wild s. avermitilis, a 1. 5 kb apramycin resistance fragment was inserted into sph i site of avec gene in the 3. 1 kb fragment, then a recombinant plasmid pc05 was obtained by introducing above inactivated avec fragment into mcs region of phjl401. competent cells of et12567 were transformed by recombinant plasmid pid03 and pc05 respectively
以含有avec基因的3 . 1kb基因組dnapsti片段為基礎，將1 . 5kb的安普黴素抗性基因片段插入到avec基因中的sphi酶切位點，再將此插入失活的avec基因片段連接到具有接合轉移功能（含有orit基因）的鏈黴菌-大腸桿菌穿梭質粒phjl401的多克隆位點區，由此得到重組質粒pc05 。On the basis of the result studied, three categories flaws easily turning up in the common whorl attachment are assayed by way of simulation. the result of experimental measurement verify all the conclusion from fem numerical simulation analysis 。 based on the study of the rationale of the ac - mfl, it primarily simulation studied the common whorl attachment in our country. the experimentation results go along with the simulation analysis
螺紋構件可以看作是柱體上緊挨的多條裂紋的組合，以上述內容為基礎，以ansys模擬軟體為手段對普通螺紋構件中極易出現的三種裂紋情況（螺桿接合部的缺陷，螺紋區的缺陷，螺紋尾部的缺陷）進行了建模模擬，得出了三種不同情況下磁場分佈特徵，實驗驗證了模擬分析的正確性。The outlet bend and water inlet connections, as well as the threaded rods to mount the plumbing facilities, are fastened at the right places, so they will stay firmly in place until final assembly
廁具彎頭進水連接件以及所需的各類連接螺桿均牢固的固定在合理的位置上，從而保證了它們在整個安裝過程中均能固定在合理的位置上。A 1. 5 kb apramycin resistance fragment was inserted into nru i site of aved gene and the inactivated aved gene fragment was then introduced into mcs region of phjl401 - an e. coli / streptomyces shuttle vector with conjugation function ( containing orit gene ). as a result of above procedures, a recombinant plasmid pid03 was obtained
將1 . 5kb的安普黴素抗性基因片段插入到aved基因中的nrui酶切位點，再將此滅活的aved基因片段插入到具有接合轉移功能（含有orit基因）的鏈黴菌?大腸桿菌穿梭質粒phjl401的多克隆位點區，由此得到重組質粒pid03 。A fragment, containing 2. 0kb cloned 5. tenebrarius dna and reported genes of erme and xyle, was inserted in plasmid phz132 ( an e. coli - streptomyces shuttle plasmid incorporating orit from rk2 ) to construct disruption plasmid pzxb0l4. the plasmid was transformed into e. coli et12567 ( puz8002 ) to construct recombinant e. coli et12867 ( puz8002, pzxb014 )
將克隆到的鏈黴菌dna2 . 0kb片段以及報告基因erme 、 xyle插入到具有orit的大腸桿菌?鏈黴菌穿梭質粒phz132中構建接合轉移質粒pzxb014 ，並將其轉入大腸桿菌et12567 （ puz8002 ）中，獲得供體菌et12567 （ puz8002 ， pzxb014 ） 。分享友人