攪拌式發酵罐 的英文怎麼說
中文拼音 [jiǎobànshìfājiàoguàn]
攪拌式發酵罐
英文
stirring typefermenting tank- 攪 : 動詞1. (攪拌) stir; mix 2. (擾亂; 攪擾) disturb; annoy
- 拌 : 動詞(攙和) stir and mix
- 式 : 名詞1 (樣式) type; style 2 (格式) pattern; form 3 (儀式; 典禮) ceremony; ritual 4 (自然科...
- 發 : 名詞(頭發) hair
- 酵 : 動詞(發酵) ferment; leaven
- 罐 : 名詞1. (罐子) jar; jug; pot; tank; tin 2. (煤礦用裝煤車) coal tub
- 攪拌 : stir; whip; agitate; mixing; puddle; [工業] rabbling; agitation; shaking down; stirring; churning
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According to these problems, we adopt to the method of mending material, optimize to fermentation media and partly ferment condition. finally, we excogitate a kind of fermentation technology that is suitable for target gene efficiency expressed and is advantageous of product purified. with the plasmid pbv220 - ifnr, pbv220 - hgfa, pbv220 - hgfb, pbv220 - hpk5 that expresses serve as the model, adopting the biostat - c15l of b. braun company, utilize the method of mending material to ferment, through optimization fermentation media and optimization partly ferment condition ( ventilate quantity, stir speed, mend material speed ), eventually establishment a kind of fermentation technology that is suitable for target gene efficiency expressed and is advantageous of product purified
以我室構建並穩定表達的重組質粒pbv220 - - ifn 、 pbv220 - hgf 、 pbv220 - hgf 、 pbv220 - hpk5為模型,分別從不同的表達宿主菌中篩選出一種適合大規模生產的菌種bl21 ( de3 ) ,該工程菌株連續傳代100代表達質粒不丟失,表達量穩定;採用b . braun公司的biostat - c15l自控發酵罐,運用分批補料技術分別進行四種工程菌的高密度發酵,通過優化工程菌發酵的培養基配方及優化部分發酵條件(通氣量、攪拌速度、補料速度) ,最終建立一種適于目的基因高效表達的高密度發酵工藝模式。
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