改組克隆 的英文怎麼說

中文拼音 [gǎizǔkèlōng]
改組克隆 英文
shuffle clone
  • 改 : Ⅰ動詞1 （改變） change; transform 2 （修改） revise; alter; modify 3 （改正） rectify; correct 4 ...
  • 組 : Ⅰ名詞1 （由不多的人員組成的單位） group 2 （姓氏） a surname Ⅱ動詞（組織） organize; form Ⅲ量詞（...
  • 克 : 克i 動詞1 （能） can; be able to 2 （克服; 克制） restrain; control 3 （攻下據點; 戰勝） overcome...
  • 隆 : 隆Ⅰ形容詞1 （盛大） grand2 （興盛） prosperous; flourishing; thriving 3 （深厚; 程度深） deep; in...
  • 改組 : reorganize; reshuffle; reorganization
  1. Recent studies show that dna transfer and recombination technology provides great potential for genetic improvement of zoysiagrasses ; molecular linkage maps were constructed from zoysia japonica and its hybrids with zoysia matrella ; the researches on gene cloning and gene resource are in progress

    最近的研究表明，基因轉移與重組技術在結縷草遺傳改良中具有巨大應用潛力；結縷草遺傳連鎖圖譜構建取得重要進展；基因克隆和基因資源研究正在展開。
  2. 2. an up - dated method was employed to purify tumv in this research. using the protease k method, we acquired the viral genome - rna. a pair of specific primers was designed and synthesized based on the nucleotide sequences of tumv coat protein genes reported before and rt - pcr was used to clone the cp genes of the six tumv isolates

    應用改進的蕪菁花葉病毒的提取方法從病葉中提取病毒粒體，應用蛋白酶k法從病毒粒體中提取病毒rna基因組，根據已報道的tumv的cp基因序列，設計併合成了一對特異引物，利用rt - pcr法克隆了6個分離物的外殼蛋白基因，與克隆載體puc19連接后通過熱激法轉化大腸桿菌dh5 。
  3. To facilitate the detection of gene replacement, apr gene was placed in the middle of the two inserts. orit from e. coli plasmid rk2 was also incoporated into the vector, therefore, pij903 derivatives can be mobilized from e. coli into streptomyces sp. fr - 008 by either bi - parental conjugation or by conjugation between streptomyces

    為了在克隆完整基因簇時避免這兩方面的影響並能隨機地獲得dna大片段，我們將第二代yac載體pjs97 - pjs98改造成為適合於在白林泉鏈黴菌基因組中dna大片段的基因置換與克隆鏈黴菌中分析被克隆大片段功能的基因置換載體。
  4. The chloroplast shsp gene was screened from the cdna library of tomato flower by pcr strategy and confirmed by sequencing. but difference was found at 3 bases of the sequence from the reported in genbank. then, an integrated vector prok ii of the chloroplast shsp gene and nptii gene ( a kanamycin resistant gene ) with camv35s promoter was constructed and introduced into tomato mediated by agrobacterium tumefaciens lba4404. transgenic tomato were screened by their ability of growing on media containing kanamycin

    本實驗採用pcr方法從番茄花cdna文庫中克隆到葉綠體shsp基因，經測序證實與genbank中已發表的序列在編碼區相差2個堿基，其中一個堿基導致1個氨基酸的改變。將葉綠體shsp基因定向克隆于帶有組成性表達啟動子camv35s的植物表達載體prok中，凍融法轉化農桿菌lba4404 ，利用葉圓盤法對番茄進行ti質粒介導的遺傳轉化。
  5. Purpose 1 construction of prokaryotic high expression vector of human platelet factor 4 ( h pf4 ) 2 expression and purification of r h pf4 3 bioassay of r h pf4 methods according to the modulation character of eukaryotic protein expression in prokaryotic cells, we design a pair of particular primers, and construct a prokaryotic expression vector pbv220 - r hpf4 by dna polymerase chain reaction ( pcr ) and dna recombinant technic. the expression plasmid was identified with pcr and dna sequencing. pbv220 - r hpf4 was transformed into e. coli dh5a, bl21 ( de3 ) and induced by increasing the temperature to 42. we identified the expression protein by sds - page and western - blotting

    目的1人血小板因子4 （ hpf4 ）原核高效表達克隆的構建2重組hpf4的表達及分離、純化工藝研究3重組hpf4的特性研究方法根據原核細胞表達真核蛋白的基因表達調控特點，設計合成一對特異引物，在pt7 - 7 - rpf4表達質粒的基礎上，應用聚合酶鏈式反應（ pcr ）對其cdna進行改造，通過dna重組技術構建成重組hpf4原核表達質粒pbv220 - rhpf4 ，用快速pcr檢測法、 dna測序分析，鑒定重組hpf4表達質粒的正確性。
  6. A top south korean university has confirmed that a team of its researchers has created the world ' s first cloned wolves, ending weeks of investigation about the team ' s alleged manipulation of data

    韓國一家名牌大學證實，該大學的一個研究小組的確培育出世界首例克隆狼，從而結束了針對這個小組篡改數據的指稱進行的數星期調查。
  7. Acetylornithine deacetylase is the key enzyme of producting l - methionine. we mainly do research work on the construction of acetylornithine deacetylase gene - engineering strain and characteristic of proteinase. in order to get high expression deacetylase strain, we obtain the gene by pcr arge gene. the product ( 2800bp ) was cloned into puc19 plasmid and confirmed with blue / white dot screening > restriction enzyme analysis and pcr. then taking the nucleotide sequencing compared with the sequence at blast of u. s. a. we constructed a high expression of gene - engineering strain - pxj 128 which containing the arge gene on the high expressing system of pxji18 with activity of acetylornithine deacetylase above 20000u / g

    為了獲得高效表達的脫乙酰鳥氨酸酶工程菌株，在工程菌技術改造及其固定化研究做了進一步的研究和探討。我們採用基因工程技術，通過pcr技術擴增出了酰化酶關鍵酶基因?脫乙酰鳥氨酸酶基因arge ，將其克隆到puc19載體中，經酶切鑒定、 pcr鑒定篩選出重組陽性質粒，並測序鑒定，通過美國blast程序進行了基因數據庫相似性比較分析。
  8. However, some of face recognition problems still require further development, this is the case for problems of recognition face images conveying changes in illumination, facial expression and changes due to the time delay between the acquistion of the reference and tested face images. our main work is to analysis methods of extraction face features and contraction of classifier. the work presented in this paper is to apply self - organizing feature map and minor component to extraction features from multi - view face images, then combine those features as a new combined feature set, in order to reduce redundancy data, we apply clone algorithms to reduce data through rotation in input space

    我們改進了一種基於矩理論的識別方法，給出了計算公式和證明過程，可用於解決小規模人臉識別問題；我們將智能方法應用到人臉識別中，分別利用自組織特徵映射和次分量方法抽取人臉的整體特徵和局部特徵，依據特徵融合理論，重新組合為新的復合特徵，為壓縮特徵數據，我們首次引入克隆選擇演算法自動進行特徵優化選擇，最後，利用支持矢量機構造多分類器進行分類識別，在不同規模人臉識別庫上模擬結果表明，該系統自適應能力強，分類識別精度高，適用於大規模復雜人臉識別問題。
  9. 3. to improve the performance of multicast tree, a rearrangement dynamical multicast routing based on clone selection is proposed

    3 、為了改善組播樹的性能，本章提出了基於免疫克隆選擇的重構動態組播路由演算法。
  10. These results suggested that the 65kda protein was a degraded product, bioassay result showed that insecticidal activity of bl - 3 was 3 - fold higher than that of bl - 2 against 3rd instar plutelh xylostella. 3. expression of cryla ( b ) gene in pseudom on a s fluoreseent pfx - 18 in order to study expression characteristics of icps gene in pseudom onas fluorescent, a new simple transformation method of pf was established

    3 .用上述重組技術獲得了含有刀口基因的lz20和含有屍2拼即「的lz20a克隆株，通過基因的誘導表達，發現p 」和pz瀏民有不同的作用機制，證明了p20具有保護cyt人溶細胞的作用，這種作用並不依賴大腸桿菌受休的不同而改變
  11. A panel from seoul national university confirmed friday that researchers did not intentionally manipulate data, but made some basic mistakes in writing the research paper on the cloned wolves

    首爾國立大學的一個專家小組星期五證實，研究人員沒有有意篡改數據，只是在撰寫有關克隆狼的研究論文中犯了一些簡單錯誤。
  12. In order to construct the recombinant plasmid pcdna3. 1 / ts87, first, the ts87gene fragment was repcred using the redesigned primers to introduce re sites of hindld and bamh i, and kozak sequence. then the rebuilt ts87 fragment was cloned into t - vector and transformed into e. coli dh5 a

    通過重新設計引物在ts87兩端加上用於構建重組質粒的酶切位點hind和bamh和用於真核表達的kozak序列。將改造后的基因片段克隆入t - vector ，轉化大腸桿菌dh5 ，通過藍白斑篩選獲得陽性克隆后測序鑒定。
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