整合蛋白 的英文怎麼說
中文拼音 [zhěnggědànbái]
整合蛋白
英文
integral protein- 整 : Ⅰ形容詞1 (全部在內; 完整) whole; all; complete 2 (整齊) neat; tidy; orderly Ⅱ動詞1 (整理; 整...
- 合 : 合量詞(容量單位) ge, a unit of dry measure for grain (=1 decilitre)
- 蛋 : 名詞1. (鳥類或龜、蛇類所產的卵) egg 2. (像蛋形的東西) an egg-shaped thing 3. (辱罵之詞)
- 白 : Ⅰ形容詞1 (似雪的顏色) white 2 (清楚; 明白; 弄明白) clear 3 (空的; 沒加他物的) pure; clear; ...
- 整合 : commensuration
- 蛋白 : 1. (卵中透明的膠狀物質) egg white; albumen; gary2. [生物化學] (蛋白質) protein
-
Intervention of two different digestion methods on the expression of surface antigen alpha 6 integrin and transferrin receptor in epidermic cells
6整合素和轉鐵蛋白受體表達的干預In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中,轉化大腸桿菌dh5菌株,篩選氨芐青霉素抗性菌落,提取質粒經酶切鑒定、 pcr分析以及確證性測序證明,所克隆的1500bp左右的片段含有完整的3abc基因,與國外參考序列相比,同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后,亞克隆3abc基因至原核表達載體ptriex - 4neo中,通過酶切鑒定、 pcr擴增以及序列分析,發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失,碰巧在3ab基因后形成一終止密碼子,但3ab基因的閱讀框架完整,選出含有3ab基因完整閱讀框架的陽性克隆,用iptg誘導表達,收集菌液進行sds - page電泳、 westernblotting分析,結果表明, 3ab基因在大腸桿菌中成功表達,其表達產物為分子量33 . 5ku的融合蛋白,並能被口蹄疫病毒陽性血清識別。經薄層掃描分析,表達量占總蛋白量的26以上。When a catalytically active enzyme forms a complex with a cofactor a holoenzyme is produced
酶蛋白和輔助因子兩者結合成完整分子時,形成具有活力的全酶。Integrin linked kinase, ilk
整合蛋白相連激酶Blast result showed the fragment containing the opuaa and its 5 " upsteam sequence was obtained
通過blast比較,表明獲得完整的atp結合蛋白基因orf框序列及其上游的核苷酸序列。Now it has been one of the most important aquatic products in the freshwater cultivation. however, this prawn ca n ' t survive at a water temperature lower than 14c, which has seriously limited its cultivation expanding. in order to obta in a new breed of this prawn with increased cold - resistance, we investigated the cloning of a synthetic gene ( sbwafp ) based on the primary sequence of the mature spruce budworm ( choristoneura fumiferana ) antifreeze protein ( sbwafp ) and the integration of sbwafp into the embryo genomes of giant freshwater prawn by spermatophore - microinjection ( smi ), a sperm - mediated gene transfer technique
本研究的特色和創新之處在於,針對羅氏沼蝦不耐低溫,但體型相對較大,精莢明顯的特點,首次將目前已知具有最強抗凍活性的雲杉卷葉蛾( sprucebudworm , choristoneurafumiferana )抗凍蛋白( sbwafp )基因( sbwafp ) ,通過精子介導的轉基因技術整合到羅氏沼蝦的胚胎中,以期培育出耐低溫的羅氏沼蝦新品系。Plant proteins can be combined to include all of the essential amino acids and form. a complete protein
植物蛋白可以結合到包括所有的必需氨基酸,並形成一個完整的蛋白質。Expression of osteopontin, integrin v subunit and 3 subunit in ectopic and eutopic endometrium in patients with endometriosis
骨橋蛋白和整合素在子宮內膜異位癥中的表達4. after having established genetic transformation system with tomato cotyledons as explant and determined the transformable of preculture time, incubation time and co - culture time, we set up the system of high frequency transformation of tomato cotyledons. then hbmp - 3m gene was transferred into tomato via agrobacterium - mqdiated transformation, and the resistant plants to hyg were obtained. by pcr analysis on part of the putative transformants, we identified that hbmp - 3m gene had been integrated into the genome of part of tomato plants. 5. transferred hbmp - 3 gene into tobacco via agrobacterium - mediated transformation and obtained the resistant plants to hyg. trans genie tobacco plants were confirmed by instantaneous expression of gus gene in calli detection, growth and bio - morphology analysis, hyg - resistant experiment and pcr analysis
通過pcr檢測證實部分番茄抗性植株中已導入hbmp - 3m基因;人骨形成蛋白一3成熟膚基因和全長基因分別轉化番茄和煙草的研究5 .通過農桿菌介導法將hbmp一3全長基因導入煙草,並且獲得了hyg抗性植株,通過gus基因瞬時表達檢測、轉化植株的生長情況及形態學分析、 hyg抗性鑒定及pc尺檢測,證明目的基因己經整合到煙草基因組中。Hla - g1, which is a newly defined non - classical hla class i molecule, plays an important role in mediating immunotolerance and protecting embryo and even some kinds of tumors from nk cells attacking. the full - length coding sequences containing cdna of hla - g1 were cloned from placenta, monocytes and liver cancer tissue of chinese donors. sequence analysis reveals that it is a highly conserved human gene with only two amino acid mutation sites compared to foreign nationality. its truncated form was overexpressed in
從中國人外周血單個核細胞胎盤組織和肝癌組織等樣品中克隆了包含完整hla - g1讀框的cdna與國外同行獲得的該基因及其蛋白質序列比較分析表明,該基因雖然有著細微的種族特異性,但高度保守並獲得了它的截斷型重組蛋白,根據蛋白一級結構和同源比較方法,模建了它及其與特異性受體kir2dl4形成復合體的空間結構模擬,預測了它們之間相互作用的特徵。According to above consideration, experiments was carried out as below : first, targeted gene - human serum albumin ( hsa ) gene was obtained via pcr technology. secondly, the hsa gene was liganded with a plasmidprla22 whose two arms carry dna sequences necessary for crossing with the human a - lactalbumin yac to form an integration vector prla - hsa, then the integration vector plasmid was co - transformated into the right yac - bearing yeast with another plasmid plrh33 which carrys a selective gene
因此,本試驗首先擴增出整合在酵母基因組里的人血清白蛋白( hsa )基因作為目的基因,並將人血清白蛋白基因插入到一個含有人-乳白蛋白yac同源序列的重組型質粒載體,以構建整合型載體,再與另一個帶篩選基因的質粒共轉化入含人-乳白蛋白yac的酵母細胞體內。The nucleotide ( nt ) sequence of the insert in phz1754 is 2299bps in size. computer assisted analysis of the sequence revealed an open reading frame ( orf ) with a g + c content of 70. 3 % that would encode a protein of 552 amino acids ( aa ). the nt seque nce comparision revealed that the orf in the sequenced region exhibits 85 % dna sequence homology with the cholesterol oxidase gene choa of streptomyces sp
對phz1754進行外切核酸酶( exonuclease , exo )順序缺失,獲得單向長度漸減重疊的系列突變體,核苷酸序列測定顯示出該ecor - sal片段的精確大小為2299bps , frameplot程序分析揭示出該區域一個完整的開放閱讀框( orf )的存在,其大小為1656bps , g + c含量為70 . 3 ,編碼552個氨基酸,利用blastsearch程序將orf的核苷酸序列及推導的氨基酸序列與因特網上基因及蛋白質數據庫進行綜合比較,發現無論在核苷酸水平還是在蛋白水平上,該orf均與膽固醇氧化酶表現出同源性,而且與鏈黴菌膽固醇氧化酶同源性最高,說明該orf編碼膽固醇氧化酶基因。It is divided to extracellular and intracellular part by transmembrane domain. there are 13 n - glycosylation sites, 20 protein kinase c phosphorylation sites, 28 casein kinase ii phosphorylation sites, 4 tyrosine kinase phosphorylation sites and 15 n - myristoylation sites in the extracellular part of bt - r3 protein. an integrin recognition sequences rod lies in intracellular part of bt - r3 protein
跨膜區域( tmd )將它分為胞內和胞外兩個部分,它的胞外有13個潛在的糖基化位點, 20個蛋白激酶c的磷酸化位點, 28個酪蛋白激酶的磷酸化位點, 4個酪氨酸酶的磷酸化位點, 15個豆蔻(十四烷基)酰化位點;它的胞內有1個整合蛋白( integrin )識別位點。The product may compose various organic acid, protein, vitamins such as matter, amino acid in beef, pig, flesh chicken, flesh duck body, promote every organ adjusting and improving an animal individual growth, raise feed digesting absorption rate, reduce feed costs, improves the quality of meat
本產品可在肉牛、豬、肉雞、肉鴨體內分泌合成多種酶類物質、氨基酸等多種有機酸、蛋白質、維生素、促生長因子,調整和提高動物各器官功能,促進個體生長,提高飼料消化吸收率,降低飼料成本,提高肉的品質。2 ) basis of upon studies, we have also designed and sythesized the mutation ii of the cmiv that been greatly changed in the 3 " of the gene comparing with nature cmiv : the ht gf3 ( the third loop region of htgf2 specifically binding to egfr receptor ) was fused to 3 " of gene of cmiv through a flexible linker. the gene of the mutation ii of cmiv was sequenced and clonged to the vector of ptxfus to fuse to the 3 " of gene of thioredoxin
二、在以上研究的基礎上,對cmiv抗菌肽的c端進行較大的改造,即將與腫瘤細胞過度表達的表皮生長因子受體( egfr )具有高親和性的因子多肽tgf _ 3通過疏水柔性接頭連接在抗菌肽cmiv的c端,設計完整的基因,並在大腸桿菌中利用ptxfus表達載體與硫氧還蛋白進行可溶性融合蛋白表達。In this paper, we review the recent research concerning the integrin activation and integrin signaling, as well as their cooperation with receptor protein tyrosine kinases
本文綜述了整合蛋白的活化過程、信號轉導過程及其與生長因子受體信號通路相互關聯研究的最新進展。By sequencing random - selected clones from the library and using bioinformatics, 54 novel expressed sequence tags ( ests ) were obtained, in which there were 14 full - length cdnas, including peroxiredoxin gene, hmg - box gene, ubiquitin gene, signal recognition particle, acetyltransferase gene and lysozyme gene
有14個克隆包含有完整的開放閱讀框,其中包括抗氧化物酶基因prx 、 dna結合蛋白基因、泛素基因、信號識別蛋白基因、溶菌酶基因和乙酰基轉移酶基因等具有重要生物學功能的基因。Integrins, a large family of receptors that attach cells to the matrix, exert an extraordinary control on cell adhesion, migration, proliferation and survival
摘要細胞外基質受體整合蛋白全方位影響細胞的形態、運動、存活和增殖。In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals
首先,用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上,並轉化e colidh5a感受態細胞中,經pcr 、酶切以及測序證明得到了完整的2c3abc基因,並與國內外參考序列進行比較分析。然後,將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ,以zeocin ~ ( tm )抗性篩選陽性克隆,大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞,通過zeocin ~ ( tm )抗生素梯度濃度篩選,獲得重組酵母用0 . 5甲醇誘導表達,通過sds - page電泳、 westernblotting分析,結果表明, 2c3abc基因在畢赤酵母中成功表達,其表達產物為一95ku的融合蛋白,並能被口蹄疫病毒陽性血清識別。Intrinsic or integral protein
內在蛋白或整合蛋白分享友人