Using indirect immuno - fluorescein staining method, the localization of sp - 10, tmdc - iii and aeg on human, mouse, rabbit, pig, bull sperm was observed under confocomicroscopy. it was found that antigens sp - 10 and aeg express on acrosome of all kinds sperms, supporting their roles in sperm - egg fusion. it was firstly described that tmdc - iii express mostly on the equator, neck and some part of tail of human sperm, on the acrosome of mouse and bull sperms, on the acrosome and neck of pig sperm, on the head, neck, and middle piece, principal piece of tail of rabbit sperm
利用間接免疫熒光法對aeg 、 sp - 10 、 tmdc -蛋白在人、小鼠、
兔子、豬、牛精子上進行了精確定位， aeg 、 sp - 10在頂體部均有表達，確證了它們與精卵融合有關：首次對tmdc -蛋白在人、小鼠、
兔子、豬、牛精子進行了精確定位，它主要存在
於人精子尾部主段，小鼠和牛精子頂體，豬精子頂體和頸部，
兔精子頭部、頸部和尾部的主段及尾段。
The greater part of the rabbit population of britain went under to the disease of myxomatosis
繁殖在不列顛的
兔子，曾經由
於粘液瘤病而死去一大半。
French physician decided to get rid of the wild rabbits on his own estate and introduced myxomatosis
一位法國內科醫生決定除掉自己莊園內的野
兔子，
於是引進了這種多發性粘液瘤疾病。
The second part, a renewable piezoelectric immunosensor is developed for the antibody of schistosoma - japonicum ( sjab ). after incubating 32 kd molecular antigens of schistosoma japonicum ( sjag ) on the qcm by applying the immobilization above, the nonspecific sites on the immunosensor are sealed by using bsa and nrs together. the immunosensor can detect the sjab with the linear range of 0. 54 ~ 32. 50 ug / ml
以感染
兔血清為檢測對象，採用聚電解質吸附固定法，將日本血吸蟲分子抗原（ siag32kd ）固定
於石英晶振表面，再以牛血清白蛋白（ bsa ）和正常
兔血清（ nrs ）聯合封閉晶振上非特異性活性位點，可在0 . 54 32 . 50ug ml范圍內檢測感染
兔血清中日本血吸蟲抗體。
It is necessary to control the mechanical stimuli precisely in the studies of cardiac mechano - electrical feedback ( mef ). in the present study a ventricular pressure - clamping system has been developed, which can be applied to isolated - perfused rabbit hearts. controlled by a computer, this system not only can make the left ventricle follow a command defining the same pressure wave as that during a beating cycle under physiological condition, but also deliver mechanical stimuli with a proper waveform to the ventricle at a particular time phase. this system integrates multiple functions, including perfusing, pacing, recording of electrocardiogram and monophasic action potentials, and clamping and measuring of ventricular pressures in isolated - perfused hearts. thus, it is a distinct system for investigating the phenomena and mechanisms of cardiac mef at organ level
在心臟機械電反饋的研究中準確控制機械刺激是非常重要的.本研究室構建了一套適用
於離體家
兔心臟的心室壓力鉗系統.該系統通過計算機控制壓力鉗，不僅能模擬正常生理條件下左心室的壓力波形，還能在心室活動周期的特定時相、以適當波形對心室施加機械刺激.該系統集心臟灌流與起搏、表面心電圖記錄、單相動作電位記錄、心室壓力鉗制與測定等多種功能
於一體，特別適用
於器官水平上觀察機械電反饋現象並探討其機制
Abstract : it is necessary to control the mechanical stimuli precisely in the studies of cardiac mechano - electrical feedback ( mef ). in the present study a ventricular pressure - clamping system has been developed, which can be applied to isolated - perfused rabbit hearts. controlled by a computer, this system not only can make the left ventricle follow a command defining the same pressure wave as that during a beating cycle under physiological condition, but also deliver mechanical stimuli with a proper waveform to the ventricle at a particular time phase. this system integrates multiple functions, including perfusing, pacing, recording of electrocardiogram and monophasic action potentials, and clamping and measuring of ventricular pressures in isolated - perfused hearts. thus, it is a distinct system for investigating the phenomena and mechanisms of cardiac mef at organ level
文摘:在心臟機械電反饋的研究中準確控制機械刺激是非常重要的.本研究室構建了一套適用
於離體家
兔心臟的心室壓力鉗系統.該系統通過計算機控制壓力鉗，不僅能模擬正常生理條件下左心室的壓力波形，還能在心室活動周期的特定時相、以適當波形對心室施加機械刺激.該系統集心臟灌流與起搏、表面心電圖記錄、單相動作電位記錄、心室壓力鉗制與測定等多種功能
於一體，特別適用
於器官水平上觀察機械電反饋現象並探討其機制
One was based on the sbs sensitive membrane and " gas phase " immunoassay technology. by immobilized anti - tb or anti - trichinosis on the sbs membrane, it can be used to determine m. tuberculosis and trfohiflosjs. the other one was based on the protein a sensitive membrane and " liquid phase " immunoassay technoiogy
一種是基
於sbs膜固定抗體的壓電氣相
兔疫技術，我們利用該技術首次對結核病和旋毛蟲病分別進行了快速診斷：另一種是基
於蛋白a定向修飾抗體的壓電液相
兔疫傳感技術，我們利用該技術首次對結核病進行了診斷。
In this paper, the relationship between cyanide - resistant respiration and expression of alternative oxidase ( aox ) using polyantibodies prepared by synthetic polypeptide in mung bean seedling, as well as the effects of vernalization on cyanide - resistant respiration and expression of aox in winter wheat were studied respectively. in the first part, twelve peptides, including eight conservative amino acid residues in the amino acid sequence of hydrophilic s helix of aox. were synthesized by solid - phase method
第一部分，我們按照交替氧化酶（ alternativeoxidase ， aox ）位
於線粒體內膜外側親水區s螺旋的氨基酸序列，用固相法合成由12個氨基酸殘基組成的多肽，將此多肽與-牛胰凝乳蛋白酶原a相連，用作半抗原，免疫家
兔制備人工合成12肽的抗體。
Conclusions ( 1 ) subacute senile mouse model can be used in immunosenesence reseach. ( 2 ) cd 137 may be an marker of aging. ( 3 ) the protection to aicd of cd137 decreased, indicate the function of cd137 was unusual. ( 4 ) the effect of d - galactose can modelling the different stage of aging. ( 5 ) the decrease of cd 137 expression on t cell from subacute senile mouse model and the aging mouse were due to the declining of their mrna. ( 6 ) the expression of cd 137 on t cells from from subacute senile mouse model and the aging mouse have a time dependently derease and the peak of cd 137 expression appeared earlier while the aging keep going on
結論（ 1 ） d -半乳糖致亞急性衰老小鼠模型t細胞cd137分子表達變化規律與自然衰老小鼠相似，造模兩個月後的大劑量組小鼠即可用
於衰老個體cd137分子的相關研究。 （ 2 ）模型小鼠cd137分子表達隨衰老的發生發展呈規律性變化，提示該分子可能為t細胞衰老的分子標志。 （ 3 ）即使在衰老狀態下，機體對cd137分于的表達依然具有調控作用，提示
兔疫系統的代償能力仍然存在。
Operation sunrise is a nonprofit international program dedicated to helping children and young people around the world who suffer from cleft lips and palates and other facial birth defects
辰光行動』是一個非贏的國際的計劃主要是致力
於幫助全球的患有
兔唇和其他的面部有先天性的缺陷的兒童和年輕人。
The last male purebred columbia basin pygmy rabbit has died, leaving just two females in a captive breeding program created to try to save the endangered species from extinction
世界上最後一隻雄性的純種哥倫比亞盆地侏儒
兔最近不幸死亡。這致使美國在實施一個旨在試圖拯救這種瀕危物種免
於滅絕的圈養繁殖計劃的過程中，僅有兩只純種的雌性侏儒
兔尚存。
There was no difference in other biologic characteristic of mscs between the two separation method, such as cell anchorage ratio and clone formation ratio. ( 2 ) plga film presented uniformity frame with no protuberance and fissure under scanning electron microscopy ( sem ). big aperture with smooth wall and average 400 m i n size running - through each other was observed in porous plga substrate, around the big aperture there were many round micropores about 5 m size. all of the structure were equal and uniform, which satisfied the further research work. ( 3 ) mscs adhesion at earlier time was promoted by biotiegenrafter 3h, cell number was ( 1. 5 0. 18 ) 105 in the plga film coated with biotiegen group, which was significantly higher than that in plga film group ( p < 0. 01 ) and higher than that in coverslip group ( p < 0. 05 ), which cell number was ( 1. 04 0. 21 ) 105. after 6h and 12h biotiegen could not promote cell adhesion, and cell proliferation and alkaline phosphatase ( alp ) activity were not promoted dramatically during 9 days. ( 4 ) cell adhesion was promoted by fibronectin or collagen type i
G ） i型膠原、纖維粘連蛋白促進細胞增殖，細胞接種后3 、 6 、 gd三個檢測時間點，實驗組細胞均明顯高
於對照組。與1型膠原相比，纖維粘連蛋白刺激作用更強。 ） i型膠原、纖維粘連蛋白尚能誘導mscs細胞向成骨細胞分化，不僅表達成骨細胞標志物ocn 、 alp 、 opnmrna ，而且堿性磷酸酶活性明顯增高，堿性磷酸酶及鈣結節7第四軍醫大學博士學位論文一染色均強陽性， i型膠原組mscs細胞堿性磷酸酶活性較fn組更高，有顯著性差異；同時，
兔疫組化染色表明，經纖維粘連蛋白作用的mscs1型膠原表達陽性。
Then the plasmid was extracted from them and determined by dna calculator. the protoplast that contain growth hormone releasing factor injected into rabbit muscle and mouse muscle after it were treated by 1 % glutaral, pay it to electric stimulation in muscle of injection site and extract omni - rna from injection site of rabbit muscle, expression of grf were detected by reverse transcription and pcr ; ratio of expression of grf were detected by elisa. extract dna form injection site of mouse muscle to research time of expression
本實驗是將含grf重組質粒的jm109菌株大量培養，用堿裂變法提取質粒，用dnacaculator定量；制備含grf的原生質體，經1的戊二醛處理后注射
於家
兔肌肉，在注射部位給予電刺激，提取總rna ，用rt - pcr檢測grf在肌肉中的表達；用elisa法定性檢測grf在肌肉中蛋白質水平的表達；將該原生質體注射于小鼠肌肉中，定期提取dna ，初步探討原生質介導的外源性grf在小鼠肌肉中的表達時間。
A hunted rabbit or deer will freeze. then the dog may not see it at all.
兔子或鹿被狗追時會「呆立不動」，
於是狗可能會一點也看不見它。
Shuangji brand back muscle machine, it suitable for cotton, cloth of korea., dripping moul - ding, cashmere, sheep wool, rabbit ' s hair, cover material leather, etc. thin and middle thick glove back muscle
雙雞牌背筋機，適用於棉毛、汗布、滴塑、羊絨、羊呢、兔毛、皮質料等薄料和中厚料手套背筋。
