昆蟲細胞培養 的英文怎麼說
中文拼音 [kūnchóngxìbāopéiyǎng]
昆蟲細胞培養
英文
insect cell culture- 昆 : 名詞1. (哥哥) elder brother2. [書面語] (子孫; 後嗣) offspring 3. (姓氏) a surname
- 蟲 : 名詞1. (蟲子) insect; worm 2. (姓氏) a surname
- 細 : 形容詞1 (條狀物橫剖面小) thin; slender 2 (顆粒小) in small particles; fine 3 (音量小) thin ...
- 胞 : Ⅰ名詞1 (胞衣) afterbirth2 (同一個國家或民族的人) fellow countryman; compatriot Ⅱ形容詞(同胞...
- 培 : 動詞1. (在根基部分堆上土) bank up with earth; earth up 2. (有目的地使成長、壯大) cultivate; foster; train
- 養 : Ⅰ動詞1 (供養) support; provide for 2 (飼養; 培植) raise; keep; grow 3 (生育) give birth to ...
- 昆蟲 : insect
- 細胞 : cell; sytes; bioplast; cella; [口語] gene; [生物學] cellule; cellule cellulli cellulo ; cello ; k...
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Yu - chan chao at the institute of molecular biology, academia sinica, constructed a recombinant baculovirus
在貼附培養的情況下,此病毒感染宿主昆蟲細胞不會造成昆蟲細胞溶裂。In the adherent culture, the virus did not result in cell lysis after the insect cells were infected
而在本論文中,我們嘗試將此一非溶裂昆蟲細胞/桿狀病毒表現系統轉移到旋轉瓶的懸浮培養。Baculovirus / insect cell system has been widely used for recombinant protein production, but traditional system eventually resulted in cell lysis, so that the expressed recombinant protein was lost into medium
摘要:桿狀病毒/昆蟲細胞系統已經被廣泛的應用在重組蛋白質的生產上,但傳統的桿狀病毒感染后會造成細胞溶裂,而使得表現出的重組蛋白質流失到培養基中。The technique of insect cell culture, one of the bases of cell engineering, is a valuable method of modern experiment biology
昆蟲細胞培養技術作為細胞工程基礎之一,是現代實驗生物學上極有價值的手段之一,廣泛應用於生物學、醫學及農業的各個領域。Review on insect cell cultivation and its application
昆蟲細胞培養及其應用進展In this experiment hcv structural gene was amplified by polymerase chain reaction ( pcr ), and was inserted into baculovirus expression vector pfastbacl to construct a recombinant transposing vector pfbl - cee. the plasmid pfb 1 - cee was transformed into dh1 obac competent e. coli cells. high molecular weight dna was prepared from the overnight cultures from the selected e. coli colonies, which was recombinant baculovirus shuttle vector containing hcv structural gene, named bac - cee
本實驗用pcr擴增hcv結構區基因,克隆到桿狀病毒表達載體pfastbacl中,構建成重組轉座載體pfb1 - cee ,轉化dh10bac大腸桿菌感受態細胞,篩選陽性菌落,抽提大分子質粒dna ,獲得含hcv結構區基因的重組桿狀病毒穿梭載體bac - cee ,脂質體介導轉染sf9昆蟲細胞,出現細胞病變后,收集含有重組桿狀病毒顆粒的培養上消,重新感染sf9細胞,收集sf9細胞,進行12 . 5 sds -聚丙烯酰胺凝膠電泳,可見表達的蛋白條帶。Development of insect cell culture media and serum - free media, establishment of insect cell lines and applications of insect cell culture for production of biopesticides and expression of recombinant proteins in genetic engineering were reviewed in this paper
摘要綜述了昆蟲細胞培養基的現狀和發展、無血清培養基的開發、昆蟲細胞系的建立以及昆蟲細胞在生物農藥和重組蛋白中的應用。分享友人