明膠培養基 的英文怎麼說
中文拼音 [míngjiāopéiyǎngjī]
明膠培養基
英文
gelatin culture medium- 明 : Ⅰ形容詞1 (明亮) bright; brilliant; light 2 (明白;清楚) clear; distinct 3 (公開;顯露在外;不隱...
- 膠 : Ⅰ名詞1 (某些具有黏性的物質) glue; gum 2 (橡膠) rubber 3 (姓氏) a surname Ⅱ動詞(用膠粘) st...
- 培 : 動詞1. (在根基部分堆上土) bank up with earth; earth up 2. (有目的地使成長、壯大) cultivate; foster; train
- 養 : Ⅰ動詞1 (供養) support; provide for 2 (飼養; 培植) raise; keep; grow 3 (生育) give birth to ...
- 明膠 : [化學] gelatin(e)
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Fos + / th + / gfap + and fos + / vp + / gfap + triple labeled n - asc could be found in the mvz, pvn and son respectively ; ( 2 ) under electronic microscope, the astrocytic processes connected closely with the dendrites or axons of the neurons, where the bilateral membranes became thick. we call transiently it electron - dense areas ( edas ). the number of edas increased remarkably following hyperosmotic stimulation ; ( 3 ) when trace retrogradely, wga - hrp was microinjected into the unilateral son, pvn or nucleus of solitary tract ( nts ) respectively using the stereotaxic method, the n - ascs formed by the neurons triple - labeled with hrp / fos / th ( or vp ) and astrocytes labeled with gfap could be found in the mvz, son and pvn respectively ; ( 4 ) after being treated with heperosmotic nacl solution, intracellular calcium concentration in cultured hypothamic neurons and astrocytes increased and then decreased
腦內gfap陽性結構也明顯增多,其分佈與fos陽性細胞分佈基本一致,表現為胞體肥大、突起粗長; ast緊密包繞在神經元周圍形成神經元- ast復合體( n - asc ) ;在mvz 、 pvn和son三重免疫組化染色切片上可見到fos + th + gfap +第四軍醫大學博士學位論文和fos vp gfap三重標記asc ; ( 2 )免疫電鏡下son內星型膠質細胞突起與神經元樹突或軸突之間接觸部位出現增厚的膜結構一電于緻密區( edas ) ,高滲刺激后數量明顯增多: ( 3 )將們個mp注入大鼠一側n卜、卜卜或孤束核( ws ) ,分別在延髓內臟帶( mvz ) 、 so和pvn內出現fos hrp th 、 fos hrp八p三重標記神經元和gfap陽性標記ast形成的n asc ; ( 4 )高滲刺激使培養神經元和ast內鈣水平先升高后降低,最後維持在比高滲刺激前稍高的靜息鈣水平上。Edible gelatine. detection of coliforms. 30 degrees c culture method on liquid selective medium
食用明膠.大腸桿菌的檢測.在30時液體選擇培養基的培養法Edible gelatine. determination of fecal coliforms. 44, 5 degrees c culture method on liquid selective medium
食用明膠.糞便中大腸桿菌的檢測.在44 . 5時液體選擇培養基的培養法The recombinant plasmid was translated into e. coli dh5 and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 22ku protein which was equal to chicken ifn - y protein in molecular weight was expressed in e. coli dh5
將重組質粒轉化大腸桿菌dh5 ,於37誘導培養8h , sds - page凝膠電泳表明該基因在大腸桿菌中獲得了高水平表達,表達的雞ifn -融合蛋白分子量約為22ku 。Result : 1 ) dmem : f12 would enhance the proliferation of hpfl, especially present with a concentration of 20ng / ml eof ; pref feeder layer can defer the differentiation of hpfl in vitro, but collagen - vi and gelatin can not do
研究結果: 1 )含有egf的dmem : fiz對于hpfl的體外增殖明顯優于其它培養液;在不同的培養基質中, pref飼養層優于明膠和膠原,可促進hpfl的生長,並能延緩hpfl在體外的分化。( 7 ) in order to establish a feeeder - free system in porcine eg culture, we put pgcs on sto feeder layer, on sto extracellular matrix or on 0. 1 % gelatin in brl - cm, and the results showed that when pgcs cultured on sto feeder, the brl - cm had a positive effect on keeping the undifferent state of eg colonies
( 7 )對非飼養層培養系統培養豬pgc進行了初步探索。實驗結果證明,以sto為飼養層,培養液中加條件培養基對豬eg細胞保持不分化狀態有積極作用,但培養板底鋪有sto基質或明膠,即使用條件培養基也沒有獲得典型的eg集落。分享友人