明膠酶 的英文怎麼說
中文拼音 [míngjiāo]
明膠酶
英文
gelatinase-
The results were as follows : leaching solution by hot water extraction was date in color and had mellow date aroma and the highest fusel oil content but strong bitter taste ; leaching solution by pectinase enzymolysis had the highest reducing sugar content easy for fermentation but excessively high methanol content in fermenting wine ; leaching solution by microwave extraction had the shortest extraction time and fermentation time and the highest ethyl acetate content in wine and the produced wine had special aroma
結果表明， 90熱水浸提，浸提液發酵酒顏色呈棗紅色，雜油醇含量最高，有濃郁棗香，但苦味重；果膠酶酶解浸提，浸提液還原糖含量最高，利於發酵，但發酵酒的甲醇含量過高；微波強化浸提，浸提時間和發酵時間最短，所得棗酒的乙酸乙酯含量最高，且有特殊香味。A new process for preparation of gelatin with enzyme
酶法制備明膠的新工藝研究Strain bl21, and gene expression was induced by iptg. the target proteins were directed into the periplasmic space by the staphylococcal protein a signal sequence preceding the rgd - hirudin gene. using ion exchange chromatography and gel filtration chromatography, the chimera proteins were purified, and both of them showed a single band in tricine - sds - page. the results of activity analysis suggested that these two chimera proteins not only have antithrombin activities, but gain platelet aggregation inhibitory activities as well
通過離子交換層析和凝膠過濾層分別對兩種嵌合體蛋白進行純化，純化產物在tricine - sds - page中都顯示為單一條帶。活性分析結果表明兩種嵌合體蛋白在保留水蛭素抗凝血酶活力的同時，還呈現抗血小板聚集活性。Mps inhibits various aggressive lysosomal enzymes that degrade important constituents of connective tissue, such as elastase ( degradation of elastin ), collagenase ( degradation of collagen ) and hyaluronidase ( degradation of hyaluronic acid )
Mps抑制了各種參與分解代謝的酶，這些酶用於降解結締組織的重要組成元素，如彈性蛋白酶（降解彈性蛋白） ，膠原酶（降解膠原）和透明質酸酶（降解透明質酸） 。The extracted marrows ganoderma lucidum and semen zizyphi spinosae contained in threesss can regulate secretion of hydrocortisone in human body and reduce excitation of human body during nighttime ; in addition, ganoderma - lucidum spores and semen zizyphi jointly stimulate secretion of sleeping - inducting peptide in body. improve rate of " high efficient sleeping quality and immunity. recover and strengthen mental strength, mental strength and vigor. through sleeping. the psychlogy and physiology return to the youth level
「索萊爾」多元明膠囊中所含的靈芝和酸棗仁提取精華，能調節人體內皮質醇的分泌，降低人體在夜間的興奮度；此外靈芝孢子粉和酸棗仁共同作用，能夠在體內刺激「睡眠肽」和格里酶「的分泌，有效促進「高能睡眠」率，延長「高能睡眠」時間，從而達到全面改善睡眠質量，提高免疫力，使體力、腦力、精力得到迅速恢復和加強，通過睡眠將生理及心理狀態調整到青春期水平。First, three isozymic systems ( lactate dehydrogenase ( ldh ), esteraes ( est ), malate dehydrogenase ( mdh ) ) extracted from threepopulations of mandarinfishes were detected by discontinuous vertical plate polyacrylamide gel electrophoresis ( page ). several loci were tested but none polymorphic locus was detected in qiupu river population. the results showed that : ldh can be used as biochemical markers to identify these three populations of mandarinfishes
首先，本試驗採用聚丙烯酰胺凝膠電泳（ page ）對我省三個水域鱖魚群體3種同工酶（ ldh 、 est 、 mdh ）的不同基因座位進行了檢測，結果表明： ldh同工酶可以作為區分秋浦河鱖魚、長江鱖魚兩個群體與萬佛湖鱖魚群體的生化遺傳標記；其次，運用rapd技術分析了三群體鱖魚的基因組dna的多態性。Having a life - threatening allergic reaction to a previous dose of mmr vaccine ; or having a life - threatening allergic reaction to gelatin or neomycin
對藥物新酶素（ neomycin ）或明膠（ gelatin ）曾有嚴重的過敏反應Based on the extensive studies of subtilisin - like protease ( prl ) of metarhizium anisopliae, extracellullar serine protease is suggested to be a key enzyme involved in the fimgal penetration to invertebrates. the investigation of serine protease in the nematode infected by owvtl may help to understand the mechanism of nematophagous fimgi as biological control agents. a 3l kda serine protease was isolated and purified from the liquid culture of h rhossiliensis owvtl challenged with nematode panagrellus redivivus
本研究利用線蟲誘導下owvt - 1菌株液體發酵，通過粗分級分離、離子交換層析和凝膠過濾層析分離提純了一個分子量為31kda的絲氨酸蛋白酶，生物學測定表明其對大豆胞囊線蟲二齡幼蟲具有致死作用，同時測定了該酶理化特性，酶活力在75附近酶活力最高，隨著ph的增加酶的穩定性升高，與膽堿酯酶具有相似的ph曲線，對特異性底物aape （ suc - ala - ala - pro - glu - pna ）具有作用， ssi和ci - 2抑制該酶的活性。There was no difference in other biologic characteristic of mscs between the two separation method, such as cell anchorage ratio and clone formation ratio. ( 2 ) plga film presented uniformity frame with no protuberance and fissure under scanning electron microscopy ( sem ). big aperture with smooth wall and average 400 m i n size running - through each other was observed in porous plga substrate, around the big aperture there were many round micropores about 5 m size. all of the structure were equal and uniform, which satisfied the further research work. ( 3 ) mscs adhesion at earlier time was promoted by biotiegenrafter 3h, cell number was ( 1. 5 0. 18 ) 105 in the plga film coated with biotiegen group, which was significantly higher than that in plga film group ( p < 0. 01 ) and higher than that in coverslip group ( p < 0. 05 ), which cell number was ( 1. 04 0. 21 ) 105. after 6h and 12h biotiegen could not promote cell adhesion, and cell proliferation and alkaline phosphatase ( alp ) activity were not promoted dramatically during 9 days. ( 4 ) cell adhesion was promoted by fibronectin or collagen type i
G ） i型膠原、纖維粘連蛋白促進細胞增殖，細胞接種后3 、 6 、 gd三個檢測時間點，實驗組細胞均明顯高於對照組。與1型膠原相比，纖維粘連蛋白刺激作用更強。 ） i型膠原、纖維粘連蛋白尚能誘導mscs細胞向成骨細胞分化，不僅表達成骨細胞標志物ocn 、 alp 、 opnmrna ，而且堿性磷酸酶活性明顯增高，堿性磷酸酶及鈣結節7第四軍醫大學博士學位論文一染色均強陽性， i型膠原組mscs細胞堿性磷酸酶活性較fn組更高，有顯著性差異；同時，兔疫組化染色表明，經纖維粘連蛋白作用的mscs1型膠原表達陽性。The purified enzyme was a monomeric protein whose optimum ph was 8. 0. inhibitor study demonstrated that it was a metalloprotease and contained sulfhydryl groups essential for catalytic activity. its activity was inhibited by divalent cations such as zn2 +, mn2 +, and the kj values of zn2 +, mn2 + were imm
這種酶溫度適應范圍較廣，在較大溫度變化區間內都具有明顯的活性，但是對緩沖溶液ph值的變化卻很敏感，體外以明膠為底物時最適溫度為38oc ，最適ph值為8The results of lauryl sodium sulfate - polyacrylamide gel electrophoreses ( sds - page ) of the aggregate precipitate and supernatant and the result of high - performance size - exclusion chromatography of the supernatant indicated that, by wrongly linked intermolecular disulfide bonds soluble bi - molecular and tri - molecular egg white lysozyme aggregate could be simultaneously formed except being renatured to native and active egg white lysozymes during the refolding procedure of denatured - reduced egg white lysozyme ; the aggregate precipitate could be further formed by the non - covalent bonds interaction between the soluble hi - molecular egg white lysozyme aggregates, and the soluble tri - molecular egg white lysozyme aggregate could still stay at the supernatant
沉澱和上清液的不連續十二烷基硫酸鈉聚丙烯酰胺凝膠電泳（ sds - page ）和高效凝膠排阻層析分析結果表明，還原脲變性蛋白溶菌酶在稀釋復性過程中除了能夠復性成天然態蛋白溶菌酶分子外，還會形成可溶的蛋白溶菌酶分子二聚體和三聚體，二聚體和三聚體主要是靠分子間二硫鍵的錯配連接而成的；可溶的蛋白溶菌酶分子二聚體之間通過非共價鍵相互作用而形成集聚體沉澱，而可溶的三聚體溶菌酶分子則仍處于復性液上清液中。Extraction of large - fragment genomic dna in order to gain dna template of pcr amplification ( long pcr amplification and salvage pcr amplification ) which was high purity and large fragment, three methods were used to extract genomic dna of bacillus subtilis, i. e. low melting - point agarose embedding method, sds - proteinase k - phenol chloroform extraction method and bacterial genomic dna extraction kit method. the genomic dna of bacillus subtilis were gained by these methods, and the operated programs of the methods were improved. the results showed that the genomic dna extracted by low melting - point agarose embedding method were obviously biggest than that of another two methods
大片段基因組dna的提取為了獲得用於pcr擴增（長距離pcr擴增和分段pcr擴增）的高純度、大片段（至少為pcr產物長度的4倍）的dna模板，應用三種方法：低熔點瓊脂糖包埋法， sds -蛋白酶k -酚氯仿抽提法和細菌基因組dna提取試劑盒法，分別提取獲得了枯草桿菌基因組dna ，並對3種方法的操作程序進行了不同程度的改進，結果表明：低熔點瓊脂糖包埋法提取的基因組dna片段明顯大於后兩種方法，採用0 . 5瓊脂糖凝膠電泳3h ，仍然跑不出加樣孔。Detection and activity analysis of gelatinases in bone and soft tissue tumors
骨和軟組織腫瘤中明膠酶的定位與活性分析Taking pig skin, pig blood, pig small intestine as raw matcerials to producepigleather, hydrolyticgelatin, hem, sod, thrombin, casing, heparin sodium etc. products elaborately and profoundly processed projedts
以豬皮、豬血、豬小腸為原料生產豬皮革、水解明膠、血紅素、 sod 、凝血酶、腸衣、肝素鈉等產品的精深加工項目。It could cleave the 18 kd peptide of psii and produce smaller peptides [ 5 ]. in order to detect the proteases in the nacl extract of psii particles, the sds - gelatin - page was applied in our experience, to identify and characterize proteases in the 1 mol / l nacl extract of spinach psii particles
為了進一步研究光系統中的各種蛋白酶，我們採用分離膠中加入0二5明膠作為底物的sds page ，對光系統顆粒及其lmol幾naci抽提液中存在的蛋白酶進行了：分離、活性檢測和性質分析。Recovered the agarose and identified by agarose gelelectrophoresis. the producys of pcr fragments and pcambial303 plasmid ligation were transformed into e. coli ( dh5a ). the result of pcrof positive recombinant and restriction analysis demonstrated that the plant expressing vector of tps is attained
Pcr產物經回收后，經瓊脂糖凝膠電泳鑒定后，與pcambia1303連接並轉化大腸桿菌dh5a ，陽性重組子經pcr和限制性內切酶酶切圖譜分析，表明已獲得海藻糖- 6 -磷酸合成酶基因的植物表達載體。The genomic dna extracted from three rubber tree strains ( pr107, rrim600, gt1 ) was digested with hinckr. the southern blot was made with the same probe as above. the result showed that all the three strains turned up only one brand ( - - 4. 8kb ). the three strains had the same inherent background
用橡膠樹pr107 、 rrim600 、 gti三個品系的基因組洲a分別經hinan酶切后，進行了southern雜交。結果表明，三個無性系都只出現一條雜交帶，位置一』致（ 4I and hindlll respectively. after the digested products were run via agarose gel electrophoresis and transferred into nylon membrane, the southern blot was carried out using the cdna of rubber tree etrl as probe. the result of the southern blot showed that a hybridization band ( - 3. 0kb ) turned up from the ecor i digested product and another band ( - 4. 8kb ) turned up from the hindi ]
從橡膠樹pr107品系的嫩葉提取基因組dna ，用限制性內切酶ecor 、 hinofll分別酶切，瓊脂糖凝膠電泳分離並轉膜后，用克隆的橡膠樹etri基因的cdna片段作探針進行southern雜交分析，結果表明， ecor酶切在約3 okb處有一條雜交帶出現， hi 。A - l was infected on monolayer sffi ther the third plaque purification to reproduce in quantity and named it a - 2. after a - 2 was infectal on sffi monolayer cell 4d harvested the cpe cell. the analysis results with sds - page and dot - elisa showed that prv vp7 gene was - - o - - abstract expressed in baculovirus
接種a - 2代病毒于sf9單層細胞上， 4d后收獲病變細胞，通過sds -聚丙烯酰胺凝膠電泳（ sds - page ）和免疫酶斑點技術（ dot - elisa ）分析，表明prv - vp7基因在桿狀病毒表達系統中得到了表達。It shows that the longest band ( 675bp ) has lower homology with flower - timing genes, but there is 80 percentage of homology with pectinesterase during citrus development, in 184 score of hsp. it is clear to see a functional domain by modeling three - dimension structure of the protein
結果發現分子量最大的片段（長675bp ）與成花計時基因同源性較低，而與植物發育過程中的果膠酶基因具有80同源性，而且局部片段對比分值達184 ；通過蛋白質三維結構預測發現，它具有明顯的功能結構域。分享友人