晶孢 的英文怎麼說
中文拼音 [jīngbāo]
晶孢
英文
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The recombinant b. thuringiensis subsp. israelensis b - pmpx2 can produce a rhombus crystalline inclusion body during its sporulation sds - page analysis proved that cytlaa had overexpressed during the sporulation of the recombinant. it was found that b - pmpx2 strain remained stable toxicity, whatever during vegetative phase or sporulation phase, which was similar to the expression of mtxl gene in the strain of protease ( s ) - deficient b. sphaericus
同時，將來源於蘇雲金芽孢桿菌以色列亞種（ b . thuringiensissubsp . israelensis ， b . t . i ）的cytlaa基因和p20伴侶蛋白基因克隆連接到質粒pmt9中，並轉化到蘇雲金芽孢桿菌無晶體型中得到重組轉化子b - pmpx2 ，電鏡觀察發現重組轉化子b - pmpx2形成一菱形晶體。The chromatographic profiles presented that these crystals all contained several compositions and each of them has the selectively herbicidal activity to the weed, which belonging to dicotyledonous weeds
菌株40012與產紫晶鏈孢囊菌（ streptosporangiumamethystogenes ）的序列相似性是99 . 37 。This thesis studied on bacillus thuringiensis strain bt886 which was separated and selected by researchers of our laboratory. according to the observation of crystal shape and the bioassay of motschulsky and fairmaire, bacillus thuringiensis strain bt886 was identified as cry3 type, and the conclusion was assured by the further study on molecular level. the 1956 base pairs full lengrh homological cry3aa gene which was toxic to motschulsky was cloned and sequenced
以該菌株為材料，克隆出了對光肩星天牛（ anoplophralabripennis （ motsch . ） ）具有毒殺作用的cry3aa同源基因，並且對該基因進行了人工改造、人工合成、大腸桿菌表達、生物活性測定、雙元表達載體的構建以及對楊樹的轉化等一系列研究，主要結果如下： （ 1 ）顯微觀察該菌株所形成的伴孢晶體為方形。After the acet is vaporized, the active substance in water is gotten. and which is vaporized at low temperature. then the crude active substance is purified by column chromatography on sephadex g - 75. after a series of purifications again, we could get some white powder at last. though the active substance is diluted to50 g / ml, the activity is still checkeded - up through phyto phtnora casicileon. the purified active substance is insensitive to heat, resistant to chloroform 、 ethanol and the orhers. in addition, the active substance is sensitive to high ph ( 10 ~ 14 ), but it is not sensitive to low ph ( 1 ~ 5 ). furthermre, when the ph is made to low again, the activity of it ' s comes back
用蒸餾水對菌體稀釋；加入適量吸附樹脂在150rpm 、 28下振蕩吸附4h ， 80 %的丙酮解吸，過濾解吸液得到活性物質的澄清溶液，旋轉蒸發儀旋轉蒸發去處丙酮，經sephadexg - 75分子篩層析得單一活性峰，收集峰值部分樣品液經冷凍乾燥得到淡褐色粉末，該活性物質用丙酮充分洗滌、甲醇-乙醚重結晶獲得略帶微黃的白色粉末，該活性物質50 g / ml仍可對蘇雲金芽孢桿菌hd - 1產生明顯的抑制作用。The parasporal inclusions consist of one or more insecticidal proteins in the form of a crystal or crystal - complex
這種類孢內含體由1 - 2個蛋白晶體或晶體復合物組成。Most susceptible insects are killed by ingestion of the crystals alone ; a mixture of spores and crystals are required for a toxic effect in only a small number of insects
大多數易感蟲種只要攝取晶體蛋白即可被殺死；只有一小部分昆蟲要求孢子和晶體同時存在。分享友人