暗黴素 的英文怎麼說
中文拼音 [ànméisù]
暗黴素
英文
nebramycin-
Arginine feeding experiment showed that nitrogen metabolism in the s. tenebraius was obviously affected by arginine through two possible ways : ( l ) pronase activity in vitro could be influnced by arginine, as a result, the catabolism of nitrogen - containing macro - molecule was promoted and the nitrogen element in the broth was increased. ( 2 ) arginine could be transformed into glutamic acid, so that the biosynthesis of apramycin was promoted
因而我們認為gln可能是安普黴素生物合成氮元素的供體。 arg添加實驗結果表明， arg可能通過兩種途徑影響黑暗鏈黴菌體內的氮代謝： （ 1 ） arg可能影響胞外蛋白酶的活性，進而促進含氮大分子物質的分解代謝，補充發酵過程中的氮素來源。6. transformation system of mustard a serials of kanamycin concentration was added to optimum medium to test the explants resistance capacity of two kinds of mustard. the transformation procedures described were derived from numerous regeneration and trasformation designed to test factors that might affect shoot regeneration, which including length of co - cultivation. those producing the best result parameters were described as below : after the mustard explants were precultured on regeneration medium for 2 days. they were inoculated with agrobacterium for 20 minutes. inoculated explants were co - cultivated for 4 days and in shadow at first 2 days. then transferred to the same medium plus 30 mg / l kanamycin and 500mg / l garb. all of them were transferred to fresh medium every 2 weeks. the kan - resistant plants were regenerated
芥菜外植體高頻遺傳轉化體系的建立在最適培養基上試驗了兩類芥菜的三種外植體對卡那黴素的敏感性、預培養天數、浸菌時間等因素的影響，建立了芥菜高頻轉基因再生體系：取生長4天的芥菜子葉、下胚軸和25天的葉片在分化培養基上（ ms + ba3 . 0mg / l + naa0 . 1mg / l ）預培養2 - 3天後，投入農桿菌菌液中浸染20分鐘，在分化培養基上暗培養2天，正常條件下培養2天後，轉入抗性培養基（ ms ba3To study the biosynthetics genes of apramycin in streptomyces tenebrarius, the apramycin resistant gene was isolated by gunshot cloning firstly
為了研究黑暗鏈黴菌安普黴素生物合成基因，首先通過鳥槍克隆的方法克隆其安普黴素抗性基因。The sensitivity to chloramphenicol of those tiny colonies and blue colonies suggested the morphological alteration was due to genome instability, which might be the secondary effect of loss - of - function of sc7c7. 05
Hxy2形念異常的子代菌落有90以上對氯黴素敏感；而表型正常的菌落100對氯黴素有抗性，暗示hxy2的表型分離現象與基因組不穩定有關。Plasmid psyxl, containing a 12kb insert of s. tenebrarius dna, was isolated from one of six colonies. apramycin resistant gene was located in 1. 5 - kb sphl - kpnl fragment of plasmid psyxl though further studies
陽性克隆中重組質粒psyx1含有12kb黑暗鏈黴菌的dna ，對12kb片段進一步分析將安普黴素抗性基因定位在1 . 5kbsphi - kpni片段上。Our experiment indicates : ( 1 ) the optimal concentration of kanamycin for screening torenia fournier regenerated buds was 400 mg / l. the ideal transformation was obtained in the following conditions : the leaf discs were dipped in agrobacterium suspension that od600 was 0. 1 for 10 ~ 20 min ; subsequently cocultivated on the ms solid coculture medium containing 20umol / l acetosyringon for 7 to 8 d at 23, and the induction ratio of regenerated buds was 27. 2 %
研究結果表明： （ 1 ）篩選藍豬耳轉化芽的最適卡那黴素濃度為400mg l 。 od _ （ 600 ）為0 . 1的菌液濃度菌液浸染葉盤10 20min ，固體共培養基（含20 mol l乙酰丁香酮）上23共培養7 8d可獲得理想的轉化效率，轉化芽誘導率為27 . 2 ； 16h光照， 8h黑暗是較理想的共培養光周期；莖段是較好的轉化受體。分享友人