木二糖 的英文怎麼說
中文拼音 [mùèrtáng]
木二糖
英文
xylobiose-
This experiment passing to grope for the carbon source constitutes of the culture medium and using t. reesei rut c - 30 induced the expression of # - mannanase ( # - 1, 4 - mannan mannohydrolase ec 3. 2. 1. 78 ). in this experiment i put the constant carbon source ( lactose and locust bean gum ) in the foundation culture medium ( mandels nourishment liquid ) of t. reesei rut c - 30, then proceeded the variable carbon source ( dragon spruce fiber, com rush pith fiber, wheat straw fiber, wheat straw xylan, corn rush pith xylan, dragon spruce mannan ) to single factor, double factor, three factor, four factor and five factor orthogonal experiment. 1 determined the activity of p - mannanase using locost bean gum as substract by the 3, 5 - dinitosalicylic acid method, and observed the growing situation of the gernic at the end i selected the directions for the inducement expression of the # ? mannanase from trichoderma reesei rut - c30 that contained the dragon spruce fiber, wheat straw xylan, dragon spruce mannan
在里氏木霉rutc - 30的基礎培養基（ mandels營養液）中加入固定碳源乳糖和槐豆膠，然後將可變碳源（雲杉纖維、玉米芯纖維、麥桿纖維、麥桿木聚糖、玉米芯木聚糖、雲杉甘露聚糖）進行單因子、雙因子、三因子、四因子、五因子的里氏木霉rutc - 30正交培養實驗，並以槐豆膠為底物用3 ， 5二硝基水楊酸法測定培養液中?甘露聚糖酶的活力。從而確定了酶活最高且菌體生長良好的含雲杉纖維、麥桿木聚糖和雲杉甘露聚糖的誘導培養基為最佳培養基，用該培養基培養的里氏木霉（ t . reesei ） rutc - 30使其轉錄的-甘露聚糖酶（ - 1 ， 4 - mannanmannohydrolaseec3 . 2 . 1 . 78 ） mrna量能夠滿足rt - pcr的要求。Genetic engineers have spent 20 years trying to create a type of yeast that can ferment xylose on an industrial scale. so far, they ' ve failed
基因工程師已花費二十年時間來嘗試製作一種酵母，它能在工業規模上使木糖發酵。但至今為止，他們並未成功。The recombinants were constructed by transforming ppic9 a - xynb into p. pastoris gs115. the assay results revealed that the xylanase gene xynb was overexpressed and secreted effectually in p. pastoris. in 3l fermentor the expression level of xylanase xynba exceeded 1200iu / ml and the expressed xylanase had normal bioactivity. the molecule weight of xynba was determined as about 31kd which is higher than 23kd of original enzyme xynb from streptomyces olivaceoviridis a1. xynbb was gotten by deglycasylation of xynba, whose molecule weight returned to 23kd. we comparised the enzymatic properties of xynba expressed in p. pastoris, xynbb deglycasylated from xynba and xynb produced from streptomyces olivaceoviridis al : there was little difference among the three enzymes on optimal ph, the optimal ph of xynb and xynba were both 5. 2, the optimal ph of xynbb was 5. 0 ; the optimal temperature of xynb and xynba were both 60 c, while the optimal temperature of xynbb was 50 ? ; because of glycosylation the thermal stability of xynba was better than xynb and xynbb ; the specific activity of xynba and xynbb were 883. 88iu / mg and 832. 5hu / mg respectively, which were both lower than 2814. 45iu / mg of xynb ; the km values of xynb and xynba were similar to each other which were 21. 56 ( g / kg ) and 20. 87 ( g / kg ), while the km value of xynbb was 27. 10 ( g / kg ) ; the fmax of xynba and xynbb were 4568umol / mg. min and 5329umol / mg. min respectively which were lower than 27623 umol / mg. min of xynb ; additionally all of the three enzymes did not display cellulase activity. they all had well resistance to pepsion and trypsin, and were not sensitive to metal iron, surface active agent and chelating agent. the analysis of different xylans enzymatic hydrolysate revealed : by xynba, that the main constitutions of enzymatic hydrolysate of birch wood xylans were xylotriose and xyloquaiose, which account for 68. 43 % and 16. 50 % respectively, additionally there was 11. 79 % of xylobiose ; the main constitutions of enzymatic hydrolysate of corncobs xylans were xylobiose and xylotriose, which account for 81. 78 % and 11. 55 %. the result indicated that this xylanase was a kind of 1, 4 - b - d - xylanohydrolase and was fit to used in industrial procession of xylooligosacc harides
進一步對xynba進行了脫糖基化處理得到xynbb ，其分子量恢復到23kd ，證明xynba是糖基化蛋白。通過對畢赤酵母重組表達的木聚糖酶xynba 、脫糖基化的木聚糖酶xynbb以及橄欖綠鏈黴菌a1所產原酶xynb之間酶學性質的比較發現：三種酶的最適ph差異不大， xynb和xynba均為5 . 2 ， xynbb為5 . 0 ； xynb和xynba的最適溫度均為60 ， xynbb降為50 ：在耐熱性上， xynba由於糖基化作用熱穩定性明顯高於未糖基化的xynb和xynbb ； xynba和xynbb的比活性分別為883 . 88iu mg和832 . 51iu mg ，明顯低於原酶的比活2814 . 45iu mg ； xynb和xynba的km值相當，分別為21 . 56 （ g kg ）和20 . 87 （ g kg ） ，而xynbb的km值較大為27 . 10 （ g kg ） ； xynba和xynbb的vmax相差不大，分別為4568 mol mg ? min和5329 mol mg ? min ，明顯低於xynb的27623 mol mg ? min此外三種酶均無纖維素酶活性，對胃蛋白酶和胰蛋白酶有很好的抗性，且對作用環境中的各種離子、表面活性劑、螯合劑不敏感。通過對不同木聚糖的酶解產物的糖份分析發現：以樺木木聚糖為底物時，酶解產物主要為木三糖和木四糖，含量分別為68 . 43和16 . 50 ，另外還含有11 . 79的木二糖；以玉米芯木聚糖為底物時，酶解產物主要為木二糖和木三糖，含量分別為81 . 78和11 . 55 。Through pathway of orientated degradation or decomposition of lignocellulosic biomass, many high - value organic substances of small molecules such as glucose, xylose, phenylpropane units and their dimers, gaseous substances such as ch4 and co, liquid substances such as organic acids, aldehydes, alcohols and other platform chemicals such as furfurals, levulinic acids, xylitols and ethanols can be produced
木質生物質通過一定的降解或分解途徑，可產生很多有重要價值的有機小分子化合物，這些有機小分子化合物有葡萄糖、木糖、苯丙烷單體及二聚體，氣態小分子如ch4和co ，液態小分子如有機酸、醛、醇，重要基礎平臺化合物糠醛、乙酰丙酸、木糖醇、乙醇等。The reducing sugar content in the phloem and xylem of populus pseudo - simonii, p. pseudo - simonii p. nigra, p. simonii p. nigra, p. koreana, 27 and 40 - year - old p. simonii wood was analyzed by 3, 5 - dinitrosalicylic acid colorimetry
摘要利用3 ， 5 -二硝基水楊酸比色法測定小青楊、小青黑楊、小黑楊、香楊、 27年生和40年生小葉楊韌皮部和木質部的還原糖含量。Thiolysis hplc will be better for the dihydrochalcones which includes the compounds of xyloglucose phloretin and phloretin, the catechins and their end units. unthiolysis hplc will be better for the hydroxycinnamic acids, procyanidin b2 and catechin monomers. 2
硫解hplc分析兒茶素類及其末端單元和二氫查耳酮（根皮苷和木糖根皮苷） ；非硫解hplc分析羥基肉桂酸類、原花青素b2 、花青素及兒茶素單體。Xylanases of the initial strain and the mutant have their optimum activity at ph 5. 4 and ph 6. 0, respectively. they have better stability in the range ph 7. 0 - 10. 0 when incubated at various phs at 45 for 2 hr. the two xylanases have maximal activity at 52 and have better stability up to 50 when incubated at various temperatures in ph 6. 0 for 30min
出發菌株在ph7 . 0到10 . 0之間木聚糖酶的穩定性較好，而突變株在ph6 . 0到10 . 0之間木聚糖酶的穩定性較好突變株和出發菌株的木聚糖酶最適作用溫度均為52 ；在20到50之間突變株和出發菌株木聚糖酶穩定性比較好，二者的半失活溫度都在55左右。分享友人