枯草蛋白酶 的英文怎麼說
中文拼音 [kūcǎodànbái]
枯草蛋白酶
英文
succinoglycan- 枯 : 形容詞1 (植物等失去水分; 乾枯) (of a plant etc ) withered 2 (井、河流等變得沒有水) (of a w...
- 草 : Ⅰ名詞1 (草本植物的統稱) grass 2 (指用作燃料、飼料等的稻、麥之類的莖和葉) straw 3 (草稿) dra...
- 蛋 : 名詞1. (鳥類或龜、蛇類所產的卵) egg 2. (像蛋形的東西) an egg-shaped thing 3. (辱罵之詞)
- 白 : Ⅰ形容詞1 (似雪的顏色) white 2 (清楚; 明白; 弄明白) clear 3 (空的; 沒加他物的) pure; clear; ...
- 酶 : 名詞[生物化學] (生物體的細胞產生的有機膠狀物質) enzyme; ferment
- 蛋白 : 1. (卵中透明的膠狀物質) egg white; albumen; gary2. [生物化學] (蛋白質) protein
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6 - phosphogluconate dehydrogenase ( 6 - pgadase, ec 1. 1. 1. 44 ) was isolated by homogenate, ammunium sulfate fractionation, deae - sepharose chromatography, blue - sepharose affinity chromatography and gel filtration with sephadex g - 200 from bacillus subtilis, and some properties of the enzyme had been studied. a 113. 8 - fold purification was obtained with a 8. 2 % yield. the purified enzyme moved as a single electrophoretic band in page
將枯草芽孢桿菌超聲波破壁后的粗提取物進行分段鹽析、 deae - sepharose陰離子交換柱層析, blue - sepharosecl - 6b特異結合柱層析和sephadexg - 200凝膠過濾等純化步驟,得到聚丙烯酰胺凝膠電泳為單一蛋白區帶,比活為1 . 46u mg的酶制劑。The results showed : protamine could inhibit the growth of " bacillus subtilis " without destroying the cellular wall and significantly inhibit the activities of succinate dehydrogenase and malata dehydrogenase
試驗結果表明:鯉魚魚精蛋白對枯草芽袍桿菌具有較強的抑制作用,但對枯草芽抱桿菌細胞壁不產生破壞作用;對黑曲?胞內的琥珀酸脫氫酶和蘋果酸脫氫酶的活性具有明顯的抑制作用。In order to investigate the antibacterial mechanism of protamine, the effect of protamine on the growth of " bacillus subtilis " and the activities of succinate dehydrogenase ( sdh ) and malata dehydrogenase ( mdh ) in cells of " aspergillus niger " was studied
通過鯉魚魚精蛋白對枯草芽抱桿菌生長的影響及對黑曲?胞內的琥珀酸脫氫酶( sdh )和蘋果酸脫氫酶( mdh )活性影響的研究,探討鯉魚魚精蛋白的抑菌機理。In order to express alkaline protease gene ( ap gene ) in bacillus subtil is, the recombinant expression plasmid was constructed. this plasmid contains a promoter bp53, also from b. pumilus un - 31 - c - 42, ap gene and the shuttle vector psugv4. after introduced into b. subtilis wb600, the transformants displayed the hydrolyzed zone on milk plate
將來自短小芽抱桿菌un一31一c礴2的基因啟動子( bp53片段)和脫毛蛋白酶全基因( ap )進行融合,然後將重組基因(命名為bpap )插入到大腸桿菌-枯草桿菌穿梭質粒載體psugv4中,構建成表達質粒psu一bpap 。- acetolactate decarboxylase are widely found among bacterial strains but not in other groups of organisms. the enzyme has been demonstrated to be effective for removal of acetolactate and widely used in beer product. in this paper, - acetolactate decarboxylase from bacillus subtilis was purifed to homogeneity from cell extract by ammonium sulfate - fractionation, heat treatment, deae - sepharose fast flow column chromatography
本文對來源於枯草芽孢桿菌( bacillussubtilis ) 3226 - 5的-乙酰乳酸脫羧酶經硫酸銨分級沉澱、熱處理、 deae - sepharosefastflow離子交換柱層析等分離純化步驟,得到sds - paeg電泳純,通過n末端氨基酸序列分析驗證酶蛋白的純度。Extraction of large - fragment genomic dna in order to gain dna template of pcr amplification ( long pcr amplification and salvage pcr amplification ) which was high purity and large fragment, three methods were used to extract genomic dna of bacillus subtilis, i. e. low melting - point agarose embedding method, sds - proteinase k - phenol chloroform extraction method and bacterial genomic dna extraction kit method. the genomic dna of bacillus subtilis were gained by these methods, and the operated programs of the methods were improved. the results showed that the genomic dna extracted by low melting - point agarose embedding method were obviously biggest than that of another two methods
大片段基因組dna的提取為了獲得用於pcr擴增(長距離pcr擴增和分段pcr擴增)的高純度、大片段(至少為pcr產物長度的4倍)的dna模板,應用三種方法:低熔點瓊脂糖包埋法, sds -蛋白酶k -酚氯仿抽提法和細菌基因組dna提取試劑盒法,分別提取獲得了枯草桿菌基因組dna ,並對3種方法的操作程序進行了不同程度的改進,結果表明:低熔點瓊脂糖包埋法提取的基因組dna片段明顯大於后兩種方法,採用0 . 5瓊脂糖凝膠電泳3h ,仍然跑不出加樣孔。Progress in research of the fibrinolytic enzyme in fermented soy sauce
海參液態發酵制備枯草桿菌蛋白酶的研究The sequence around these residues revealed that ap was a new member of the subtilisin family
該蛋白酶可以認為是枯草桿菌蛋白酶家族( subtilisin )的新成員。分享友人