核外粒子 的英文怎麼說
中文拼音 [héwàilìzi]
核外粒子
英文
extra nuclear particle-
In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中,轉化大腸桿菌dh5菌株,篩選氨芐青霉素抗性菌落,提取質粒經酶切鑒定、 pcr分析以及確證性測序證明,所克隆的1500bp左右的片段含有完整的3abc基因,與國外參考序列相比,同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后,亞克隆3abc基因至原核表達載體ptriex - 4neo中,通過酶切鑒定、 pcr擴增以及序列分析,發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失,碰巧在3ab基因后形成一終止密碼子,但3ab基因的閱讀框架完整,選出含有3ab基因完整閱讀框架的陽性克隆,用iptg誘導表達,收集菌液進行sds - page電泳、 westernblotting分析,結果表明, 3ab基因在大腸桿菌中成功表達,其表達產物為分子量33 . 5ku的融合蛋白,並能被口蹄疫病毒陽性血清識別。經薄層掃描分析,表達量占總蛋白量的26以上。The influence of the cloud droplet spectrum character and the spectrum growth and change is considered, which does not use a cut - off value for conversion from cloud water into rain water process. in the continuous coalescence equation, the particles fall velocity difference is not moved out from the integral, but is integrated in the equation as the function of diameter d to avoid the error of using particles average fall velocity. in the new scheme, generation rate of graupel due to the collection of snow by graupel and the collection of cloud ice by graupel are included
雲中凝結核ccn的數濃度採用超幾何函數表示;雲水向雨水的自動轉換過程採用grabowski ( 1999 )的公式,考慮了雲滴譜的特徵和發展變化對該過程的影響,而不是採用原方案給定閾值的方法描述該過程;對連續碰並方程不再將粒子落速差作為常量提出積分號外,而是直接作為粒子直徑函數在積分號內求解,這樣處理可以迴避使用粒子群的平均落速帶來的誤差;增加了霰和雪、霰和冰晶的碰並微物理過程。Hollow ferrites particles are the alternative to conventional solid ferrites particles, which have lower density, larger specific surface area and stability. moreover, it is expected electromagnetic wave can be reflected and absorbed again and again in the cavum of hollow particles which become its black hole. in addition, their electromagnetic parameters can be adjusted in a certain range by changing the thickness of the shell and the diameter of hollow microspheres, and the ability of absorbing microwave can be expected to improve
空心結構的鐵氧體是對傳統實心鐵氧體的改造,不僅密度小,還具有特殊的電、磁功能,有可能使電磁波在其空腔中反復振蕩吸收,成為電磁波的「黑洞」 ,從而增加材料的吸波能力,同時可通過調節核殼粒子的內外徑來調控電磁參數。These particles constitute the cores of the interstellar dust grains that may be coated with ice.
這些粒子構成了星際塵埃顆粒的核子,這些塵埃顆粒的外面也許覆蓋一層冰。In our culture condition, the lepcs express oval cell markers ck 19, ck 14, ov6 and oc. 10, but not oc. 2 and oc. 5. the cells also express c - met, the receptor for hepa tocyte growth factor ( hgf ). antigens traditionally associated with haematopoietic stem cells, including c - kit, thy - 1 and cd 34, can be expressed by oval cells
該細胞系是典型的上皮樣細胞,體外生長時成「鋪路石」樣排布;在電鏡下觀察,細胞核質比大,胞漿中除一些線粒體和核糖體外缺乏其他細胞器;在體外培養時細胞可以保持不分化狀態,表達卵圓膽管細胞的分子標志,如ck14 , ck19 , ov6 , ocBased on this point, our aim to obtain the high performance materials should be carried out as well as the er behavior be actualized through the chemistry design. in this paper, 4 supramolecular complexes of - cyclodextrin inhydrosoluble polymer / l - ( 2 - pyridlazo ) - 2 - naphthol, 6 hydrosoluble - cyclodextrin polymer / 3 - hydroxy - 2 - naphthoic acid, 2 - cyclodextrin - starch resins and 6 supramolecular complexes of - cyclodextrin - starch copolymer / substituted salisylic acid and 3 - hydroxy - 2 - naphthoic acid er particles were prepared, respectively. the structure of these particles was characterized by ir, nmr, uv - vis, the fluorescence analysis, element analysis, and etc, respectively
在本項研究中,分別制備了4種不溶性-環糊精聚合物及其與1 - ( 2 -吡啶偶氮) - 2 -萘酚構築的超分子配合物、 6種水溶性-環糊精聚合物及其與3 -羥基- 2 -萘甲酸的超分子配合物、 2種-環糊精澱粉樹脂及6種-環糊精澱粉共聚物與取代水楊酸、 3 -羥基- 2 -萘甲酸形成的超分子配合物顆粒,通過紅外光譜、熒光分析、元素分析、電子吸收光譜及核磁共振譜等對它們的結構進行了表徵。Plasmids are exta-chromosomal doublestranded, circular dna molecules found in prokaryotic and eukaryotic cells.
質粒是在原核和真核細胞中發現的染色體外的雙鏈環狀DNA分子。H. moreover, high density is required to increase the chance of collision. hence, the reactions can only take place in the core of the sun, with temperature over ten million degrees 10
首先核聚變需要極高的溫度,讓氫原子核能有足夠的能量克服原子核之間的電排斥力,此外亦需要極高的密度去增加粒子碰撞的機會,所以核聚變只能在溫度高達10Mutated plasmid was transformed into e. coli tg1 cells to produce engineered peptide, then the peptide was purified by cm sepharose ion - exchange column. in vitro bactericidal assay and drug withdrawal were used to identify the bioactivity of the engineered peptide. the planar lipid bilayer membrane was used to assay the electrophysiology of the engineered peptide. toxicity studies on mammalian cells were used to assay the toxicity of the engineered peptide
將重組質粒轉化入大腸桿菌tgi工程菌中,生產構建的工程多膚,離子交換純化后獲得工程多膚初步純化產物,體外抗菌試驗、藥物撤離試驗檢測工程多膚的抗菌活性,在人工脂質膜上測定其形成離子通道的特性以初步研究抗菌機理, ?並觀察其對真核細胞的毒性作用。Nuclear scattering results in the displacement defects in material as well as the deflection of proton from its incident direction ; electronic stopping of protons acts as the most important factor in the degradation of incident proton energy, resulting in electronic effects such as single event upset
核散射是導致入射質子運動方向改變以及缺陷產生的主要因素,入射質子與核外電子云的作用是高能質子在材料中慢化的主要因素。核反應在宇宙高能質子引起的單粒子效應中有重要影響。In this article, the advanced structure of hybrid peptide mae is predicted with software. the fused gene mae - intein - cbd is amplified by pcr with the template of plasmid ptyb2, and then it is cloned into expression vector plasmid ppic9k. after verified by restriction enzyme analyzing and sequencing, the vector is transferred into the eukaryotic host ( yeast pichia
並以已構建的載體ptyb2 - mae為模板,通過pcr擴增出融合基因mae - imein - cbd ,將其克隆于表達質粒ppic9k中,通過鑒定並測序正確后,電轉化真核表達宿主? ?畢赤酵母菌株gs115 ,通過營養缺陷型培養基篩選重組子,再利用g418抗性篩選出整合有多拷貝外源基因的重組子。Moreover, the calculations of the valence neutrons for the single - particle states of 49ca and 209pb show that the neutron halos or skins can exist in the region of heavy nuclei
對49ca和209pb各單粒子激發態外層中子的計算表明:在中重和重核區存在中子暈、皮核。Plasmids are exta - chromosomal doublestranded, circular dna molecules found in prokaryotic and eukaryotic cells
質粒是在原核和真核細胞中發現的染色體外的雙鏈環狀dna分子。In this article, the misgurin gene and adaptor are synthesized according to the amino acid sequence reported in the genebank and the need of construction and expression. adopted a new strategy, multiple copy gene is ligated in the same direction. and then it is cloned into expression vector plasmid ppic9k
本文根據genebank登錄的氨基酸序列,同時考慮構建和表達的需要,化學合成了misgurin基因和接頭,採用一種新的策略,在體外將基因多拷貝同向串連,並將其克隆于表達質粒ppic9k中,通過鑒定並測序正確后,電轉化真核表達宿主? ?畢赤酵母菌株gs115 ,通過營養缺陷型培養基篩選重組子,再利用g418抗性篩選出整合有多拷貝外源基因的重組子。The study on the core - shell particles shows, one extrenity of silane coupling agents at the surface of the composite particles after adding aps are connected with the fe particles. and the other extrenity outside can polymerize with the active silica. the slick and compact surface of the composite particles shows the obvious core - shell structure. the surface of the composite particles are composed of the combined apex of the element si, o, c, h. the element o is superfluous to the element si
對納米復合鐵粒子研究表明,在aps改性的鐵納米粒子表面硅烷偶聯劑氨基一端連接鐵粒子,另一端對鐵粒子外面,能與活性硅聚合。鐵納米復合粒子顆粒表面光滑緻密。核殼復合粒子表面主要由si , o組成,伴隨著微量的c , h元素的峰,核殼復合粒子表面氧對硅有富餘。Cd 14 * monocytes or bone marrow derived hematopoietic progenitor cells cultured with granulocyte - macrophage colony - stimulating factor ( gm - csf ) and il - 4 differentiate into efficient apc with morphology and cell surface molecule expression typical of immature dc. it was demonstrated that a variety of factors ( such as lps, tnf - a, il - 1, monocyte - conditioned medium, and cd40 receptor cross - linking ) induce the maturation of immature dc to mature dc that are much more potent in activating t cells by increasing the expression of costimulatory molecules and cytokines, promoting the migration of dc to draining lymph nodes, and down - regulating the capacity of dc to capture and process antigen
在體外,外周血cd14 ~ +單核細胞和cd34 ~ +骨髓造血前體細胞在粒細胞-巨噬細胞集落刺激因子( granulocyte - macrophagecolony - stimulatingfactor , gm - csf )和il - 4的共同作用下可分化為未成熟dc ,未成熟dc具有強大的攝取和處理抗原的能力,但其抗原提呈能力較弱;未成熟dc在炎性細胞因浙江大竿俗士學位論文潛廷平子汐ilq , yfax病原成分汐lpsx單核細胞條件培養液或cd40l等作用下可分化為成熟dc 。Positrons and neutrinos are produced during the process, and are the telltale trace of the nuclear fusion reaction inside the sun
V ,那就可以和另外一粒質子結合,釋放能量。這過程產生正電子及中微子,它們是太陽內部核融合反應的印記。To investigate the silence effect of hela cells " telomerase gene expression after shrna based on human telomerase htert transfected into cells. methods : we constructed a partial double - strand dna with t7 promoter as dna template and synthesized small hairpinrna in - vitro using t7 rna polymerase
方法:根據端粒酶htert基因1573 ? 1591位的核酸序列,構建帶t7啟動子的部分雙鏈dna模板,用t7rna聚合酶體外合成短鏈shrna 。This same relationship may be observed in the nucleus and surrounding electrons within an atom. and these subatomic particles are in turn made up of spinning sub - particles
我們從觀察原子的結構,也可以看到這種組成分子與整體的關系,原子是由原子核與外圍的電子組成,而這些原子粒子也是由一些旋轉的次原子粒子所組成。分享友人