核外粒體 的英文怎麼說

中文拼音 [héwàilìtǐ]
核外粒體 英文
cell granule
  • 核 : 核構詞成分。
  • 外 : Ⅰ名詞1 （外面） outside; external side 2 （外國） foreign country 3 （以外） besides; beyond; in ...
  • 粒 : Ⅰ名 （小圓珠形或小碎塊形物） small particles; grain; granule; pellet Ⅱ量詞（用於粒狀物）
  • 體 : 體構詞成分。
  1. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  2. In vitro study of peripheral blood polymorphonuclear leukocyte degranulation induced by the filtrate of ultrasonic pulverization from actinobacillus actinomycetemcomitans

    伴放線放線桿菌超聲粉碎濾液誘導外周血中多形核白細胞脫粒的體外研究
  3. The seven sisters that dr de grey wishes to slaughter with sens are cell loss, apoptosis - resistance ( the tendency of cells to refuse to die when they are supposed to ), gene mutations in the cell nucleus, gene mutations in the mitochondria ( the cell ' s power - packs ), the accumulation of junk inside cells, the accumulation of junk outside cells and the accumulation of inappropriate chemical links in the material that supports cells

    德?戈瑞博士想用這一策略對付的七個因素是：細胞丟失，細胞凋亡阻抑（細胞拒絕程序死亡的傾向） ，細胞核內的基因突變，線粒體（細胞的「動力倉」 ）中的基因突變，細胞內的垃圾堆積，細胞外的垃圾堆積和細胞支持性物質中不當化學鍵堆積。
  4. During the stages of primary and secondary spermatogonium, components of the nucleolus, called chromatoid bodies ( cb ), are excluded from the nucleus

    精原細胞階段，部分核仁物質外排，成為擬染色質小體，其上聚集一群線粒體，構成「線粒體區」 。
  5. Hollow ferrites particles are the alternative to conventional solid ferrites particles, which have lower density, larger specific surface area and stability. moreover, it is expected electromagnetic wave can be reflected and absorbed again and again in the cavum of hollow particles which become its black hole. in addition, their electromagnetic parameters can be adjusted in a certain range by changing the thickness of the shell and the diameter of hollow microspheres, and the ability of absorbing microwave can be expected to improve

    空心結構的鐵氧體是對傳統實心鐵氧體的改造，不僅密度小，還具有特殊的電、磁功能，有可能使電磁波在其空腔中反復振蕩吸收，成為電磁波的「黑洞」 ，從而增加材料的吸波能力，同時可通過調節核殼粒子的內外徑來調控電磁參數。
  6. In our culture condition, the lepcs express oval cell markers ck 19, ck 14, ov6 and oc. 10, but not oc. 2 and oc. 5. the cells also express c - met, the receptor for hepa tocyte growth factor ( hgf ). antigens traditionally associated with haematopoietic stem cells, including c - kit, thy - 1 and cd 34, can be expressed by oval cells

    該細胞系是典型的上皮樣細胞，體外生長時成「鋪路石」樣排布；在電鏡下觀察，細胞核質比大，胞漿中除一些線粒體和核糖體外缺乏其他細胞器；在體外培養時細胞可以保持不分化狀態，表達卵圓膽管細胞的分子標志，如ck14 ， ck19 ， ov6 ， oc
  7. Thus, immunologists have sought smaller molecules with the antigen - recognition capability of antibodies. the variable domains of the heavy ( h ) and light ( l ) chains are sufficient for antigen recognition but the non - covalent complex of the two variable domains ( fv ) is unstable. it is possible to genetically engineer a single - chain fv ( scfv ) with the h chain v region connected to the l chain v region via a 15 amino acid linker composed of serine and glycine amino acid residues

    二、日本血吸蟲單克隆杭獨特型抗體np30的單鏈狐（ scf ）的構建、表達及對balbic小鼠誘導保護性作用研究l 、日本血吸蟲單克隆杭獨特型抗體np0的單鏈抗體歸cfv ）的構建、表達通過pcr方法體外擴增並經測序驗證的重鏈、輕鏈可變區（ vh 、 vl ）基因先後重組入原核表達質粒ptha90相應的位點上，中間通過一連接肽（ gly在er ） 。
  8. Plasmids are exta-chromosomal doublestranded, circular dna molecules found in prokaryotic and eukaryotic cells.

    質粒是在原核和真核細胞中發現的染色體外的雙鏈環狀DNA分子。
  9. Mutated plasmid was transformed into e. coli tg1 cells to produce engineered peptide, then the peptide was purified by cm sepharose ion - exchange column. in vitro bactericidal assay and drug withdrawal were used to identify the bioactivity of the engineered peptide. the planar lipid bilayer membrane was used to assay the electrophysiology of the engineered peptide. toxicity studies on mammalian cells were used to assay the toxicity of the engineered peptide

    將重組質粒轉化入大腸桿菌tgi工程菌中，生產構建的工程多膚，離子交換純化后獲得工程多膚初步純化產物，體外抗菌試驗、藥物撤離試驗檢測工程多膚的抗菌活性，在人工脂質膜上測定其形成離子通道的特性以初步研究抗菌機理， ?並觀察其對真核細胞的毒性作用。
  10. In this article, the advanced structure of hybrid peptide mae is predicted with software. the fused gene mae - intein - cbd is amplified by pcr with the template of plasmid ptyb2, and then it is cloned into expression vector plasmid ppic9k. after verified by restriction enzyme analyzing and sequencing, the vector is transferred into the eukaryotic host ( yeast pichia

    並以已構建的載體ptyb2 - mae為模板，通過pcr擴增出融合基因mae - imein - cbd ，將其克隆于表達質粒ppic9k中，通過鑒定並測序正確后，電轉化真核表達宿主? ?畢赤酵母菌株gs115 ，通過營養缺陷型培養基篩選重組子，再利用g418抗性篩選出整合有多拷貝外源基因的重組子。
  11. Section four : effects of copper and cadmium on ultrastructure of myocardial cell in sinopotamon yangtsekiense the effect of copper and cadmium on ultrastructure of myocardial cells of sinopotamon yangtsekiense was studied by us

    銅、鎘聯合作用30d后，細胞核形態進一步改變，內外膜分離程度加大，異染色質疑集加重。線粒體膜破裂，內容物外流，嵴斷裂消失。
  12. Plasmids are exta - chromosomal doublestranded, circular dna molecules found in prokaryotic and eukaryotic cells

    質粒是在原核和真核細胞中發現的染色體外的雙鏈環狀dna分子。
  13. In this article, the misgurin gene and adaptor are synthesized according to the amino acid sequence reported in the genebank and the need of construction and expression. adopted a new strategy, multiple copy gene is ligated in the same direction. and then it is cloned into expression vector plasmid ppic9k

    本文根據genebank登錄的氨基酸序列，同時考慮構建和表達的需要，化學合成了misgurin基因和接頭，採用一種新的策略，在體外將基因多拷貝同向串連，並將其克隆于表達質粒ppic9k中，通過鑒定並測序正確后，電轉化真核表達宿主? ?畢赤酵母菌株gs115 ，通過營養缺陷型培養基篩選重組子，再利用g418抗性篩選出整合有多拷貝外源基因的重組子。
  14. Two application softwares, the nmr calibration software and the fast nmr core analysis software, are programmed. the two softwares offer the specific calibration and fast measurement function of cuttings petrophysical parameters. the research results show that nmr technique has the advantage of fast, non - contact and independence of samples size and can be applied to the cuttings analysis

    研究結果表明，核磁共振分析技術具有快速、無接觸、與樣品外形體積無關的特性，不僅可以應用於規則形狀的巖心樣品，同樣可以較好地應用於不規則的小顆粒樣品，如巖屑的分析。
  15. Cd 14 * monocytes or bone marrow derived hematopoietic progenitor cells cultured with granulocyte - macrophage colony - stimulating factor ( gm - csf ) and il - 4 differentiate into efficient apc with morphology and cell surface molecule expression typical of immature dc. it was demonstrated that a variety of factors ( such as lps, tnf - a, il - 1, monocyte - conditioned medium, and cd40 receptor cross - linking ) induce the maturation of immature dc to mature dc that are much more potent in activating t cells by increasing the expression of costimulatory molecules and cytokines, promoting the migration of dc to draining lymph nodes, and down - regulating the capacity of dc to capture and process antigen

    在體外，外周血cd14 ~ +單核細胞和cd34 ~ +骨髓造血前體細胞在粒細胞-巨噬細胞集落刺激因子（ granulocyte - macrophagecolony - stimulatingfactor ， gm - csf ）和il - 4的共同作用下可分化為未成熟dc ，未成熟dc具有強大的攝取和處理抗原的能力，但其抗原提呈能力較弱；未成熟dc在炎性細胞因浙江大竿俗士學位論文潛廷平子汐ilq ， yfax病原成分汐lpsx單核細胞條件培養液或cd40l等作用下可分化為成熟dc 。
  16. To sum up, a recombinant plasmid pcdna3. 1 / ts87 was constructed and expressed successfully in eukaryotic cells, which has laid a strong basis on further studies of t. s vaccine

    L ts87重組質粒，並在體外真核細胞中成功表達，為進一步體內實驗提供了有力依據。
  17. A series of core - shell nanoparticles with own intellectual property have been prepared, which provides theory for the magnified synthesis of core - shell nanoparticles. in part two, the biocompatibility of the core - shell nanoparticles, including silica nanoparticles, fluorescent silica nanoparticles and silica coated magnetic nanoparticles, have ' been investigated from the angle of in vivo and ex vivo

    第二，從體內和體外兩個角度系統地研究了核殼納米顆粒（主要包括二氧化硅納米顆粒（ sinp ） 、二氧化硅殼熒光納米顆粒（ fsinp ）以及二氧化硅殼磁性納米顆粒（ msinp ） ）的生物親和性，證明了該類納米顆粒有很好的生物親和性。
  18. To investigate the silence effect of hela cells " telomerase gene expression after shrna based on human telomerase htert transfected into cells. methods : we constructed a partial double - strand dna with t7 promoter as dna template and synthesized small hairpinrna in - vitro using t7 rna polymerase

    方法：根據端粒酶htert基因1573 ? 1591位的核酸序列，構建帶t7啟動子的部分雙鏈dna模板，用t7rna聚合酶體外合成短鏈shrna 。
  19. This same relationship may be observed in the nucleus and surrounding electrons within an atom. and these subatomic particles are in turn made up of spinning sub - particles

    我們從觀察原子的結構，也可以看到這種組成分子與整體的關系，原子是由原子核與外圍的電子組成，而這些原子粒子也是由一些旋轉的次原子粒子所組成。
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