核毒素 的英文怎麼說

中文拼音 [hédúsù]
核毒素 英文
nucleotoxin
  • 核 : 核構詞成分。
  • 毒 : Ⅰ名詞1 （對生物體有害的性質或物質; 毒物） poison; toxin 2 （毒品） drug; narcotics 3 （姓氏） a s...
  • 素 : Ⅰ形容詞1 （本色; 白色） white 2 （顏色單純） plain; simple; quiet 3 （本來的; 原有的） native Ⅱ名...
  • 毒素 : 1. [生物學] （有機體產生的有毒物質） toxin 2. （言論或著作中有腐蝕作用的成分） poison
  1. Translocation from endocytic compartments to the cytosol is the essential and rate - limiting step in the intoxication process of most toxins such as ricin, diphtheria toxin, shiga toxin and pseudomonas exotoxin ( pe ). a number of these toxins are transported to trans - golgi network ( tgn ), and in many cases such transport to the tgn is required for the translocation and cytotoxicity. in deed, 5 % of the ricin endocytosed by cells has been shown to reach the tgn

    蓖麻毒素進入細胞的機理不甚明了，一般認為是rtb先與細胞膜受體結合，主要經過受體介導的內吞作用進入吞噬體，然後沿著內體、高爾基體、內質網等逆向分泌途徑，有序地運輸到內質網，最後從內質網轉位進入細胞漿，在胞漿內攻擊核糖體，從而抑制蛋白質的合成，導致細胞死亡。
  2. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  3. In this study five recombinant plasmids containing hn ( hemagglutinin - neuraminidase ) genes of ndv ( newcastle disease virus ) were constructed, hn genes were amplified by reverse transcriptase - polymerase chain reaction ( rt - pcr ) and were inserted directly into eukaryotic expression plasmid pcdna3

    應用反轉錄-聚合酶鏈反應（ rt - pcr ）獲得兩株新城疫病毒（ ndv ）血凝素-神經氨酸酶（ hn ）基因cdna ，然後定向插入真核表達質粒pcdna3中，構建了含hn基因的重組質粒。
  4. Expression of thermostable direct hemolysin gene of vibrio parahaemolyticus in e. coli and activity of expressed product

    副溶血性弧菌直接溶血毒素基因的原核表達及產物的性質
  5. Their outside individual nucleus cell is in week blood the leucocyte interferon that small amount produces only after virus is atttacked

    他們的外周血單個核細胞在病毒攻擊后僅產生低量的白細胞干擾素。
  6. Construction of adenoviral vector for luciferase driven by htert core promoter modified with myc - responsive elements

    核心啟動子引導熒光素酶表達的腺病毒載體
  7. Cytochrome p450 functions as the monooxygenase. p450s are involved in physiologically important processes including steroid metabolism, drug deactivation, procarcinogen activation, fatty acid metabolism, xenobiotic detoxification and wildly distributed in animal, plant and low eukaryotic organism

    細胞色素p450具有加單氧酶的作用，並能參與機體解毒、甾體激素的合成、脂肪酸代謝等重要的生理反應，並廣泛分佈於動物、植物和低等真核生物中。
  8. This paper is a study on the expression of the protective gene of bont / a in the prokaryotic and eukaryotic. it indicates the possibility to produce the recombinant protein in quantities. it has also laid down a good foundation for the further research on vaccine or antibody of bont / a

    本論文進行了人工合成的a型肉毒毒素hc段基因在原核和真核表達系統中的表達研究，使制備大量bont a保護型抗原的重組蛋白成為可能，為進一步進行a型肉毒毒素的疫苗或抗體研究奠定了一定的基礎。
  9. The research adopts that hu - - ifn gene were introduced into the nuclei of oocytes or cytoplasm of grass carp to develop anti - disease transgenic grass carp breeding researches, combing the adva ntag e of hu - - ifn gene and breeding by genetic engineering, with an aim of finding out an effective way of solving antivirus of hemorrhagic virus of carp completely. in research of transgenic fish, hu - - ifn gene ( recombination gene ) is cutdown and introduced into the nuclei of oocytes or cytoplasm of grass carp at one - cell or two - cell stage via micyoinjection by narashige micyoinjection apparatus

    本研究的目的在於以人的-干擾素基因（ ifn - ）作為目的基因，與鯉魚-肌動蛋白基因啟動子在體外重組，利用原核顯微注射轉基因技術將人-干擾素基因導入草魚基因組而開展的抗病轉基因草魚育種研究，其結合了干擾素和基因工程育種抗草魚出血病病毒的優點，以期獲得對草魚出血病具有天然抗性的轉基因魚，並在此基礎上培育出草魚抗病新品系。
  10. The present results indicated that the paraventricular nucleus of the hypothalamus and the supraoptic nucleus might have important roles in neuroimmunomodulation. 2. following lps or seb was administered intraperitoneally, the expression of pcna of splenic cells and il - 1 receptor type i in pvn and son were observed by using immunocytochemistry in the mice. double fluorescent labeling technique was used to determine the relationship of il - 1 receptor type i co - expressions with arginine vasopressin or oxytocin

    二、小鼠腹腔內給予細菌內毒素lps或腸毒素seb ，用免疫組織化學方法觀察了脾臟核增殖抗體及下丘腦室旁核和視上核中1型il 1受體的表達，並採用雙標記技術觀察了1型il刁受體陽性神經元和加壓素及催產素表達的關系。
  11. Abnormalities of abrs were analyzedandinterpreted as peripheral hearing loss ( wave i prolongation ), neurological brainstem involvement ( interwave i - v prolongation or absence of later components ) or both

    為?解膽紅素的神經毒素，本文先就核黃疸病人的聽性腦干反應提出初報。
  12. In the experiments discussed in chapter 5 we generated two recombinant viruses based on an acmnpv - and hasnpv - bac - to - bac system, respectively. in such recombinant viruses the busuctl gene under polyhedrin promoter was inserted into polyhedrin gene locus. a preliminary bioassay was conducted

    第五章利用桿狀病毒bac - to - bac系統構建了含有油桐尺蠖核多角體病毒的類蝸牛毒素基因的重組病毒racbacctl和rhabacctl ，在其相應宿主甜菜夜蛾和棉鈴蟲的細胞水平和蟲體水平進行了超表達實驗。
  13. Inhibition or enhancement test of bacterial endotoxins of cefquinome injection

    卡介菌多糖核酸制劑的細菌內毒素檢查法
  14. Part one the studies of piezoelectric biosensors and detection of staphylococcal enterotoxins chapter one the progresses in piezoelectric biosensors and detection of staphylococcal enterotoxins section one the progresses in piezoelectric biosensors in this section, a review is reported with 69 references about the piezoelectric biosensors, including the principle and constitutes of pebs, the classification of pebs, surface modification technique in pebs and the application of pebs in immunoassay, detection of the gene and microorganism, coupling with other techniques, moreover, the development of pebs was involved

    引用參考文獻69篇。第二節金黃色葡萄球菌及其毒素檢測研究進展該部分內容主要介紹了在葡萄球菌分類與分型、金黃色葡萄球菌毒素學研究以及金黃色葡萄球菌及其毒素檢測等方面的研究進展，重點對金黃色葡萄球菌及其毒素檢測方法：生物學檢測方法、免疫血清學方法、聚合酶鏈反應技術、核酸雜交技術、生物傳感器技術和超抗原技術在近幾年的研究進展做了評述，引用文獻69篇。火nxia即。
  15. Therefore, the determination of seb is very important for food hygienic analysis as well as for clinical analysis. nucleic acid hybridization technique is one of the widely - used methods in molecular biology and gene technology. the present work has developed piezoelectric biosensors used in the detection of seb dna by tacking the piezoelectric quarts crystal as a sensitive component while synthetic oligonucleotide probe as recognize molecule

    其中b型葡萄球菌腸毒素（ seb ）是一種通常條件下更穩定，毒性最強的毒素，而核酸雜交技術則是分子生物學和基因工程中最常用和最基本的方法之一，因此本論文以該毒素的產毒基因為檢測對象，以壓電石英晶體為敏感元件，以合成的寡核苷酸探針為識別分子，構建了用於seb基因檢測的壓電生物傳感器。
  16. It is known from studies using brefeldin a ( bfa ) that disruption of tgn can block the cytotoxicity of ricin, suggesting that the intracellular compartment may be an important part of the uptake pathway. ricin enters the cells by receptor - mediated endocytosis, followed by translocation across the membranes of intracellular organelles. in this study, a trans - golgi retention peptide signal yqrl was fused to the c - terminus of ricin a chain ( rta ) by polymerase chain reaction

    本實驗為重點研究反式高爾基體在毒素細胞內轉運過程中的作用，我們設計了一個反式高爾基體的靶向信號肽基因yqrl （ tyr - gln - arg - leu ）連接在rta分子的羧基端，用原核表達蛋白體系，純化並測定rta - yqrl與rta蛋白對hela （人宮頸癌細胞） ， wish （人羊膜上皮細胞） ， skov - 3 （人卵巢癌細胞）三種細胞的毒性作用。
  17. Isolation and purification of four main peptide toxins from a. fuligiea were undergone by means of rp - hplc, and idenfication of the chemical structure of - amanitin and - amanitin by nuclear magnetic resonance ( nmr ) showed that the the chemical structure were the same as the reference

    對灰花紋鵝膏菌的四種主要肽類毒素進行了分離純化，並用質譜和核磁共振對兩種鵝膏毒肽（ - amanitin 、 - amanitin ）進行了結構鑒定。結果與文獻報道的相一致。
  18. The compound showed effects on isolated atria, although the structure of this compound is different from the ach, the bioactivities of them are similar to each other

    1 .義摘』絲潛、核磁共振進行對照，三者差別很人。據此我們推斷該毒素分子可能為一種擬膽堿類的新成分。
  19. These anthraquinones are extremely cytotoxic ( they fight against ) towards a broad spectrum of bacteria, fungi, and viruses that include pneumonia, e coli, blood infections, diarrhea, skin infections and ( tuberculosis

    蒽醌是一種極為有效的廣譜類抗細胞毒素物質，可抗擊多種細菌、真菌、病毒，包括導致肺炎的細菌和病毒、大腸埃希氏桿菌，以及導致血液感染、腹瀉、皮膚感染和肺結核的微生物。
  20. Such humans may take on large causes in the years ahead, taking stands for peace, equality, toxic cleanup, dismantling of nuclear armaments, and end to the brutality towards nature, vegetarianism, and so on ; pushing the humans species towards a new system founded upon equality with all species upon earth

    這些人會承擔前方年份中的大致因，肩負和平、平等、凈化毒素、解除核彈武裝、終結對大自然的野蠻、素食主義等的立場，並將人類物種推向建立地球萬物平等的新系統中。
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