核熒光 的英文怎麼說

中文拼音 [héyíngguāng]
核熒光 英文
nuclear fluorescence
  • 核 : 核構詞成分。
  • 熒 : 形容詞[書面語]1. （光亮微弱的樣子） glimmering 2. （眼光迷亂; 疑惑） dazzled; perplexed
  • 光 : Ⅰ名詞1 （照耀在物體上、使人能看見物體的一種物質） light; ray 2 （景物） scenery 3 （光彩; 榮譽） ...
  1. More and more fluorescent signal can be collected with the pcr reaction carry on. the method is more automatized and much less time consumption ( only 3 hours from nucleotide hybridization capture to result found )

    這樣利用熒光信號積累可以實時監測整個pcr進程，實現了pcr擴增和核酸雜交以及熒光電信號放大檢測同步進行的自動化檢測技術；實時熒光pcr技術具有更大的優越性： l ）不需要pcr后處理，
  2. The main equipment here is a thermal cycler system for nucleic acid amplification ( pcr, quantitative fluorescence detection or common )

    主要儀器就是核酸擴增熱循環儀（ pcr儀，實時熒光或普通的） 。
  3. To investigate the consequence of this interaction, aes - rfp fusion protein expression vector was constructed and co - transfected into nih 3t3 cells with tle1 - gfp fusion protein expression vector. confocal microscopy observation showed that aes could interact with tle1 at the cytomembrane region. moreover, this interaction inhibited the concentration of tle1 into nucleus

    在構建了紅色熒光蛋白aes表達載體后，將其與tle綠色熒尤蛋白載體共轉染細胞，共聚焦顯微鏡觀察發現這兩種分子在胞漿中有共存現象，而且aes的表達可抑制tlei向胞核內的聚積。
  4. Application of hantavirus nucleocapsid protein into immunofluorescence assay

    漢坦病毒核蛋白在免疫熒光診斷技術中的應用
  5. Construction of adenoviral vector for luciferase driven by htert core promoter modified with myc - responsive elements

    核心啟動子引導熒光素酶表達的腺病毒載體
  6. Tumor necrosis factor ( tnf ), a cytkine that exerts many pro - atherosclerotic effects in vivo, causes up - regulation of acat - 1 gene expression in human blood monocytes. by luciferase activity assay, tnf - a enhanced acat - 1 p7 promoter activity in thp - 1 monocytic cell line

    細胞因子tnf -即腫瘤壞死因子大量存在於人動脈粥樣硬化斑塊中，本實驗通過熒光素酶活性和rt - pcr測定結果表明， tnf -處理人血單核細胞和單核細胞株thp - 1均增強acat - 1基因p7啟動子活性。
  7. It was showed under the laser scanning confocal microscopy that : for dna level fish, 81 % of the dnas were in the nucleoli and at the periphery of the nucleoli and 19 % in the nucleoplasm ; for rna level fish, 22 % of the rnas were in the nucleoli, 78 % in the nucleoplasm and at the boundary between it and the nucleoli ; for dna - rna level fish, 25 %, 46 % and 29 % of the dnas or rnas were in the nucleoli, at the periphery of the nucleoli and in the nucleoplasm, respectively

    結果如下： dna水平熒光原位雜交結果顯示， 81的dna位於核仁內部及其核仁周邊區域， 19的位於核質中； rna水平熒光原位雜交結果表明， 22的rna位於核仁內， 78的位於核質及與核仁交界處； dna - rna水平熒光原位雜交結果是， 25的dna或rna位於核仁內， 46處于核仁周邊， 29位於核質中。由此推測出， rna聚合酶的轉錄主要發生在核仁及其周邊區域。
  8. We used fission yeast schizosaccharomyces pombe ( s. pombe ), an unicellular eukaryotic organism, as research material. electroporation was adopted to load ca2 + fluorescent indicator into yeast cell and under the laser scanning confocal microscopy ( lscm ), we observed cytosolic ca2 + distribution and relative content as well as fluorescence intensity of gfp - cam in different phases of cell cycle of yeast cell. flow cytometry provided a way of determining the relative dna content of populations of fission yeast

    本文以單細胞的真核模式生物裂殖酵母（ schizosaccharomycespombe ）為研究材料，通過激光掃描共聚焦顯微鏡觀察酵母細胞胞質內游離ca ~ （ 2 + ）的分佈及相對濃度，以及不同周期時相細胞中gfp - cam的熒光強度變化，並採用細胞流式法對酵母細胞的相對dna含量進行測定以確定細胞所處周期時相。
  9. The sucking mouse brain were inoculated with mdj - 01 strain to make electron microscopic examination, results showed that the virus was a spheral particle with membran which had a diameter of about 40 nm. by indirect fluorescent antibody test mdj - 01 strain was identified with tbev. a part of region encoding e protein was expanded by rt - pcr and sequenced. the nucleotide sequences of two strain viruses were compared with sequences in genbankjsequence homology analyses revealed mdj - 01 strain and senzhang strain had the highest homology with tbev oshima5 - 10, respectively, which were 95 %, 94 %. mdj - 01 strain was identified with tbev again

    應用間接免疫熒光試驗進行血清學鑒定，結果表明mdj - 01株為tbev 。通過rt - pcr技術擴增部分e蛋白序列並測序，在genbank上進行同源性比較，發現mdj - 01株和森張株與tbevoshima5 - 10株的同源性最高，分別為94 、 95 ，從分子生物學水平上進一步證明mdj - 01株病毒為tbev 。在鑒定的基礎上，本實驗對兩株病毒進行了核苷酸全序列測定。
  10. The loop sequence of mb1 and mb2 were the anti sense and sense sequence ofing1, respectively the sequence of mb3 was a piece of ssrna sequence in tobacco mosaic virus, which had no analogical to human gene. mbl was the most suitable probe because mbl had the highest fluorescence enhancemen after hybridizing wtth rna extrated froin normal cell

    第三章，根據一種常見的病毒煙草花葉病毒（ tmv ）的核酸序列設計了分子信標熒光探針，由於tmv的遺傳物質是rna ，分子信標又具有很高的特異性和靈敏度，因此感染了病毒粒子的植物葉片在經過簡單處理后，可用分子信標檢測葉片上。
  11. Oxygen - evolving psil core complexes treated with hydrophilic crosslinker edc showed a little change in the microenvironments of tyr and trp residues. however, oxygen - evolving psil core complexes treated with hydrophobic crosslinkers such as dcc, hmdi, egs, and dtsp showed a distinctly change in both the microenv

    Egs 、 dtsp對psll放氧核心復合物蛋白質中tyr 、 trp微環境和682urn處葉綠素熒光影響大，可能它們參與了psll放氧核心復合物內部的蛋白疏水區域交聯。
  12. The ps ii native fractions ( 20 % and 30 % ) were loaded onto a deae column. the fraction eluted with 150 mm nacl was presented dcip reduction activity and was highly depleted in chi c and xanthophylls, and as such could be considered a ps ii core complex

    對于有dcip光還原活性的20和30層帶的復合物，進一步deae離子交換層析純化。 150mmnacl洗脫純化后的樣品經過熒光激發光譜測定發現，已經去除了葉綠素c和墨角藻黃素，並且仍然具有dcip的光活性，分析是ps核心復合物。
  13. By compressing a monolayer film, the coexistence of liquid condensed ( lc ) and liquid expanded ( le ) phases can be reached. the transition from le to lc is usually regarded as a first - order one, so the theory of crystallization can be applied. in this article we review our recent studies on the growth of lc domains in the le - lc coexistence region driven by the illumination of a fluorescent microscope. the mechanism of this unusual 2d domain growth phenomenon is discussed. the formation of faceted, dendritic and fractal - like domains as well as the evolution and the transition of these patterns are investigated

    當處于氣液界面的類脂類化合物的單分子膜被壓縮時，隨著分子間距的縮小，單分子膜將經歷一系列相變過程.通過熒光顯微術可以觀測到新相的成核和生長過程.由於單分子膜的二維特性，該系統中的實驗觀測對于檢驗和發展二維界面生長理論尤為重要.本文總結了近年來本課題組與相關單位合作，在單分子膜系統中發現的實驗現象以及對其生長機制的系列研究.內容包括對單分子膜系統中的成核、界面穩定性、枝晶生長、形態演變等的觀測和分析
  14. Abstract : by compressing a monolayer film, the coexistence of liquid condensed ( lc ) and liquid expanded ( le ) phases can be reached. the transition from le to lc is usually regarded as a first - order one, so the theory of crystallization can be applied. in this article we review our recent studies on the growth of lc domains in the le - lc coexistence region driven by the illumination of a fluorescent microscope. the mechanism of this unusual 2d domain growth phenomenon is discussed. the formation of faceted, dendritic and fractal - like domains as well as the evolution and the transition of these patterns are investigated

    文摘:當處于氣液界面的類脂類化合物的單分子膜被壓縮時，隨著分子間距的縮小，單分子膜將經歷一系列相變過程.通過熒光顯微術可以觀測到新相的成核和生長過程.由於單分子膜的二維特性，該系統中的實驗觀測對于檢驗和發展二維界面生長理論尤為重要.本文總結了近年來本課題組與相關單位合作，在單分子膜系統中發現的實驗現象以及對其生長機制的系列研究.內容包括對單分子膜系統中的成核、界面穩定性、枝晶生長、形態演變等的觀測和分析
  15. 2. to establish a neutrajizing - inhabition time - resolved fluoroimmunoassay ( trfia ) of hepatitis b virus e antibody ( hbeab ) and hepatitis b virus core antibody ( hbcab ) based on the competion. the assay ranges of standard cures were 0 - 16ncu / mland 0 - 8ncu / ml. the within - run coefficient variations for standard samples were less than 10 %. compared this method with radioimmunoassay, the sensitivities were 92. 8 % and 93. 3 %

    以中和抑製法建立了乙型肝炎病毒e抗體時間分辨熒光免疫分析法（ hbeab - trfia ）和乙型肝炎病毒核心抗體時間分辨熒光免疫分析法（ hbcab - trfia ） ，標準曲線分析范圍分別為0 - 16ncu ml和0 - 8ncu ml 。
  16. In this paper, the tunable optical characteristics, preparation for quantum dots, the value of core / shell structure, the advantages of quantum dots as fluorescent labeling, quantum dots biolabeling techniques and their application in life science were reviewed, and the future prospects was envisioned

    摘要從量子點的光學特徵、制備、核殼結構的意義、量子點熒光標記物的優越性、量子點標記生物分子后在單個細胞及臨床組織樣品檢測中的應用等方面綜述了量子點在生命科學領域的研究進展。
  17. Advances of fluorimetric determination of dna

    熒光光度法測定核酸的研究進展
  18. Production of transgenic embryos expressing green fluorescent protein by nuclear transfer from different types of somatic cells in pig

    不同類型的轉基因細胞為核供體生產豬的轉綠色熒光蛋白基因克隆胚胎
  19. . from the direct mutant of spirulina platensis ( sp - d ), we got high purity and activity phycobiliprotein which could grow crystals. the algae fluorescent probe prepared by coupling the above polyclonal antibody to phycobilipotein not only keeps the property of stronger anti - fluorescence quenching but also has the lower fluorescent background when it was used for labeling stoma cells of pea tendril

    以原核表達的peac1為抗原制備了免疫活性較好的抗豌豆肌動蛋白的多克隆抗體，從螺旋藻中純化了高純度、高活性、能結晶的藻膽蛋白，將兩者偶聯制備的藻熒光探針，不僅保持了藻膽蛋白很強的抗熒光淬滅能力，而且用於豌豆卷須氣孔細胞熒光標記時有更低的熒光背景。
  20. The obtained recombinant - 5 - htr plasmid was tranfected into human liver cancer smmc - 7721 cells. all data suggested the expression of plncx - atr could condense cell nucleus and increase nuclear fluorescence intensity, effectively repress the telomerase activity, cell growth and cell proliferation, and induce cell apoptosis

    反義重組質粒plncx - atr轉染人肝癌smmc - 7721細胞，結果發現plncx - atr的表達有效地封閉或抑制肝癌細胞的端粒酶活性，使細胞的生長和增殖受到抑制，細胞體積變小、核緻密、核熒光強度增強，且促進其凋亡。
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