核苷酸對 的英文怎麼說
中文拼音 [hésuānduì]
核苷酸對
英文
nucleotide pair-
In this study, iltv - nm98a strain and iltv - wanggang strain were multiplied in chorioallantois. a pair of primers were devised according to the nucleic acid sequence of iltv tk gene and the dna of multiplied virus was used as pattern to amplify the gene of tk by polymerase chain reaction ( pcr ). the product of pcr was linked with suitable plasmid. then, the recombined plasmid was converted to escherichia coli. the converted escherichia coli
根據已發表的iltvtk基因的核苷酸序列設計一對pcr引物，以增殖的兩株iltv的dna為模板，分別對它們的tk基因進行pcr擴增。將回收的pcr產物連接到適當的質粒載體上，轉化感受態大腸桿菌，通過篩選對iltvtk基因的陽性克隆進行擴增培養。Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l. cv hetao flesh, which was cloned into plasmid vector pmd - 18 - t. the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed. at the base that pollination and fertilization of cucumis melo l. cv hetao was studied, using pollen tube pathway transformate cucumis melo l. cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed
本實驗以河套蜜瓜果肉基因組dna為模板，用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增，得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上，篩選反向克隆，然後將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子「 」的下游，構建成反義表達載體pbinya 。並在對河套蜜瓜授粉受精生物學研究的基礎上，通過花粉管通道法轉化河套蜜瓜，共獲76顆瓜，並進行了硬度和含糖量的分析。S is the concentration of unpaired nucleotides.
S是不配對的核苷酸的濃度。The study results of this paper can serve the two scientific subjects of our teaching and research group as basic data calculation and elementary exploration. the two subjects are : constructing high activity - amylase genes by dna shuffling technology and studying on the evolution in vitro by mutational pcr ( with 5 - br - dutp as substitute partly ) and dna shuffling technology. - amylase ( ec 3. 2. 1. 1 ; 1, 4 - a - d - glucanohydrolase ) can catalyzes the hydrolysis of - 1, 4 - glycosidic bonds of starch from middle and liberates - maltose, - glucose and - limit dextrin stepwise
本試驗根據genbank已公布的黃單胞菌-澱粉酶基因的核苷酸序列由引物設計軟體premierprimer5 . 0輔助設計了一對引物（ primer & primer ） ，以pbluescript ks +和puc18 / puc19質粒為載體，用常規的pcr方法從xanthomonascampestrispv . malvacearum （ smith ） dye等七株黃單胞菌（ xanthomomasspp . ）的基因組dna中克隆得到8個基因片段，分別命名為zhyf001 zhyf008 。As bases are added by polymerase to the starting point of a new complementary strand, known as a primer, or recognized by ligase as a match, the template ' s sequence is revealed
當聚合酶將一個核苷酸加在新互補鏈的起始引子之後，或接合酶認定某段核苷酸鏈與原始模版配對，就可利用這些反應來得出原始模版的序列。All kind of oligonucleotides ( 16 sense primers, 8 antisenset primers, 128 different primer combinations in all ) were designed from the conserved motifs glpl and cfly ( distance between them is approximately 210bp ) which existed only in the type of nbs - lrr resistance gene and used as primers for scanning the genomic dna. no specific products ( which is approximately 210bp in length and obtained from genomic dna containing ht gene ) was obtained though amplified products of approximately 500 - looobp appeared in six different primer combinations
依據nbs - lrr類r基因特有的兩個保守序列glpl和cfly （兩者相距約70個氨基酸）合成所有可能的簡並寡核苷酸引物（左引物16個，右引物8個，共組成128個引物組合）對玉米基因組dna進行擴增的結果表明：在6個引物組合中得到了擴增產物，產物長度在500 1000bp之間；沒有獲得特異性擴增產物（指僅從含ht基因材料中得到，長度約210bp的dna片斷） 。G gene of rabies virus m, the of two main regions ( about 1000nt ), ranging from 3161nt to 4162nt and ranging from 4012nt to 4863nt of glycoprotein gene of rabies virus strain m, isolated from mouse in he nan, china were amplified by reverse transcriptase - polynerase chain reaction ( rt - pcr ) in order to complete glycoprotein gene of strain m. these regions were sequenced by the produce of pcr directly. comparison and analysis of nucleotide sequence and amino acid sequence deduced with that of other strains published was performed by computer with dnasisv 2. 5demo software
本研究對我國河南某地野鼠體內分離的狂犬病野毒株mrv基因的3161位? 4162位（ 1001個堿基）和4012位? 4863位（ 851個堿基）片段進行了反轉錄pcr擴增和序列測定，得到mrv的糖蛋白基因全序列，用dnasisv2 . 5demo分析軟體，與已發表的代表性毒株g基因全序列進行核苷酸和氨基酸序列的比較分析，結果表明在同一基因型中， mrv和國際標準攻毒株cvs的同源性最高（ 96 . 5 ） ，和中國減毒株ctn的同源性最低（ 79 . 8 ） 。Reduced nicotinamide nucleotides can function as reductants to nitrogenase.
還原的煙酰胺核苷酸對固態酶可以起還原劑的作用。Researchers can figure out the evolutionary relations of opsins in the various classes of cones and from different species by examining the sequences of nucleotide bases ( or dna “ letters ” ) in the genes that code for these proteins
透過比對視蛋白基因的核苷酸鹼基（或稱dna字母）序列，研究人員就能找出不同錐細胞視蛋白以及不同物種間視蛋白的演化關系。Total of 1030 aligned sites were obtained, including 346 variable and 259 parsimony informative sites
序列比對后約1030bp ，含346個核苷酸變異位點， 259個簡約信息位點。The sucking mouse brain were inoculated with mdj - 01 strain to make electron microscopic examination, results showed that the virus was a spheral particle with membran which had a diameter of about 40 nm. by indirect fluorescent antibody test mdj - 01 strain was identified with tbev. a part of region encoding e protein was expanded by rt - pcr and sequenced. the nucleotide sequences of two strain viruses were compared with sequences in genbankjsequence homology analyses revealed mdj - 01 strain and senzhang strain had the highest homology with tbev oshima5 - 10, respectively, which were 95 %, 94 %. mdj - 01 strain was identified with tbev again
應用間接免疫熒光試驗進行血清學鑒定，結果表明mdj - 01株為tbev 。通過rt - pcr技術擴增部分e蛋白序列並測序，在genbank上進行同源性比較，發現mdj - 01株和森張株與tbevoshima5 - 10株的同源性最高，分別為94 、 95 ，從分子生物學水平上進一步證明mdj - 01株病毒為tbev 。在鑒定的基礎上，本實驗對兩株病毒進行了核苷酸全序列測定。S is the concentration of unpaired nucleotides
S是不配對的核苷酸的濃度。Through the analysis of the nucleotide and ammo acid of the sequences about e2 gene, it express that the e2 gene includes about 1100bp which encodes about 370 amino acides
通過對pbne2p e _ 2基因的進一步基因序列和核苷酸序列分析，結果表明， e _ 2基因包括約1110個堿基，編碼約370個氨基酸。Inhibitory effects of heparinase antisense oligodeoxynucleotides on growth of human malignant melanoma trans planted subcutaneously in nude mice
乙酰肝素酶反義寡核苷酸對人惡性黑素瘤裸鼠皮下移植瘤抑制作用的研究Effects of antisense oligonucleotide on expression of tissue factor in culturec human umbilical vein endothelial cells injured by anoxia - reoxygention
反義寡脫氧核苷酸對內皮細胞缺氧再復氧損傷組織因子表達的影響The exprossion of vascular endothelial growth factor and microvessel density in glioma
血管內皮生長因子及其反義寡核苷酸對膠質瘤血管形成的影響Effect of et - 1 antisense oligodeoxynucleotide on the hemodynamics of normal and experimental hypertensive rats
內皮素- 1前體基因反義寡核苷酸對正常和高血壓大鼠血流動力學的影響Inhibition of the activation and collagen production of cultured rat hepatic stellate cells by antisense oligonucleotides against transforming growth factor
1反義寡核苷酸對大鼠肝臟星狀細胞激活及其膠原生成的抑制作用Inhibitory effects of antisense oligonucleotides targeting mitogen - activated protein kinase mapk mrna on neonatal rat cardiac fibroblast proliferation induced by ang and egf
反義mapk寡核苷酸對ang及egf誘導的心肌成纖維細胞增殖的抑制效應Effects of nuclear factor - b decoy oligodeoxynucleotide on the function of human umbilical artery smooth muscle cells induced by umbilical sera in preeclumpsia
順式誘騙寡脫氧核苷酸對子癇前期患者臍血清培養的臍動脈平滑肌細胞功能的影響分享友人