Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l. cv hetao flesh, which was cloned into plasmid vector pmd - 18 - t. the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed. at the base that pollination and fertilization of cucumis melo l. cv hetao was studied, using pollen tube pathway transformate cucumis melo l. cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed
本實驗以河套蜜瓜果肉基因組dna為模板,用甜瓜acc氧化酶基因特異寡
核苷酸鏈為引物進行pcr擴增,得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上,篩選反向克隆,然後將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子「 」的下游,構建成反義表達載體pbinya 。並在對河套蜜瓜授粉受精生物學研究的基礎上,通過花粉管通道法轉化河套蜜瓜,共獲76顆瓜,並進行了硬度和含糖量的分析。
S is the concentration of unpaired nucleotides.
S是不配對的
核苷酸的濃度。
Cytidine ( cytosine nucleoside ) a nucleoside formed when cytosine is linked to dribose via a - glycosidic bond
胞嘧啶
核苷:當胞嘧啶通過糖
苷鍵與d
核糖接合形成的
核苷。
All of our products is exported likelactobacillus acidophilus, bidifobacterium longum, lactobacillus paracasei, lactobacillus rhamnosus, tagatose, lnulin, nisin, natamycin, - polylysine, nucleotides mix, adenosine monophosphate, cytidine monophosphate, guanosine monophosphate disodium, uridine monophosphate disodium, calcium inosinate, calcium guanylate, calcium 5 - ribonucleotide, damp, dcmp, dgmp, tmp, deoxyadenosine, 2, 6 - diaminopurine nucleoside, 2, 6 - diaminopurine deoxynucleoside, adenine arabinoside, adenosine triphosphate, uridine triphosphate, guanosine triphosphate, cytidine triphosphate, cytidine diphosphate choline ( citicoline ) to all over the world, if you are interested in our products, please do visit our website, hope there will fullfill your requirement
主營產品有益生菌(嗜酸乳桿菌、長雙歧桿菌、乾酪乳桿菌、鼠李糖乳桿菌) 、塔格糖、菊粉(菊糖) 、乳酸鏈球菌素、納他黴素、 -聚賴氨酸、
核苷酸,腺
苷酸,胞
苷酸,鳥
苷酸二鈉,尿
苷酸二鈉,肌
苷酸,鳥
苷酸鈣,
核糖
核苷酸鈣,脫氧腺
苷酸,脫氧胞
苷酸,胸
苷酸二鈉,脫氧鳥
苷酸二鈉,脫氧腺
苷, 2 -氨基脫氧腺
苷, 2 -氨基腺
苷,阿糖腺
苷,胞二磷膽堿,腺
苷三磷酸,三磷酸尿
苷,三磷酸胞
苷,三磷酸鳥
苷等
All of our products is exported like nucleotides mix, adenosine monophosphate, cytidine monophosphate, guanosine monophosphate disodium, uridine monophosphate disodium, calcium inosinate, calcium guanylate, calcium 5 - ribonucleotide, damp, dcmp, dgmp, tmp, deoxyadenosine, 2, 6 - diaminopurine nucleoside, 2, 6 - diaminopurine deoxynucleoside, adenine arabinoside, adenosine triphosphate, uridine triphosphate, guanosine triphosphate, cytidine triphosphate, cytidine diphosphate choline ( citicoline ) to all over the world, if you are interested in our products, please do visit our website, hope there will fullfill your requirement
主營產品有
核苷酸,腺
苷酸,胞
苷酸,鳥
苷酸二鈉,尿
苷酸二鈉,肌
苷酸,鳥
苷酸鈣,
核糖
核苷酸鈣,脫氧腺
苷酸,脫氧胞
苷酸,胸
苷酸二鈉,脫氧鳥
苷酸二鈉,脫氧腺
苷, 2 -氨基脫氧腺
苷, 2 -氨基腺
苷,阿糖腺
苷,胞二磷膽堿,腺
苷三磷酸,三磷酸尿
苷,三磷酸胞
苷,三磷酸鳥
苷等
The sugar in all the nucleotides of dna is deoxyribose.
所有DNA的
核苷酸中的糖均是脫氧
核糖。
The sugar in all the nucleotides of dna is deoxyribose
所有dna的
核苷酸中的糖均是脫氧
核糖。
Deoxyribose a pentose ( five - carbon ) sugar that is a component of the nucleotides that make up dna
脫氧
核糖:組成dna
核苷的一種戊糖(五個碳) 。
The study results of this paper can serve the two scientific subjects of our teaching and research group as basic data calculation and elementary exploration. the two subjects are : constructing high activity - amylase genes by dna shuffling technology and studying on the evolution in vitro by mutational pcr ( with 5 - br - dutp as substitute partly ) and dna shuffling technology. - amylase ( ec 3. 2. 1. 1 ; 1, 4 - a - d - glucanohydrolase ) can catalyzes the hydrolysis of - 1, 4 - glycosidic bonds of starch from middle and liberates - maltose, - glucose and - limit dextrin stepwise
本試驗根據genbank已公布的黃單胞菌-澱粉酶基因的核苷酸序列由引物設計軟體premierprimer5 . 0輔助設計了一對引物( primer & primer ) ,以pbluescript ks +和puc18 / puc19質粒為載體,用常規的pcr方法從xanthomonascampestrispv . malvacearum ( smith ) dye等七株黃單胞菌( xanthomomasspp . )的基因組dna中克隆得到8個基因片段,分別命名為zhyf001 zhyf008 。