核表型 的英文怎麼說

中文拼音 [hébiǎoxíng]
核表型 英文
nuclear phenotype
  • 核 : 核構詞成分。
  • 表 : Ⅰ名詞1 （外面;外表） outside; surface; external 2 （中表親戚） the relationship between the child...
  1. By physiognomy feature, it could be divided into three types of thermal structure : positive dome model, negative collapse model and border dome core collapse model. based on the depth degree or magma - thermal influenced, it could be divided into five types of thermal structure : ( ancient ) geothermal anomaly focus region model, superficial volcano eruption hydro - thermalism and hypabyssal intrusive model, thermal anticline ( thermal dome ) model, mid - deep intrusive model, deep mantle ( crust ) thermal plume model ; and put forward a perfect model of the thermal structure. there are many interaction system could be induced into a systematic thermal interaction, include : ocean - continent system, basin - mountain interaction, superficial and mid - deep crust - mantle interaction, crust - mantle commingle interaction, vertical thermal interaction ( delamination ) etc.

    依據地貌形態分為三類：正向穹窿型、負向塌陷型、邊隆核陷型：依據巖漿-熱力作用影響的深淺程度或深度分為五類： （古）地熱異常群集區、表淺層火山噴發-熱液活動與淺成侵入型、熱力背斜（熱穹窿） 、中深層侵入型、深部地幔（地殼）熱柱型；提出了熱力構造作用空間分佈的理想模式，將洋陸系統、盆山作用、淺表與中深部殼幔作用、殼幔混合、垂向熱力作用（拆沉）等納入一個整體統一的熱力作用系統中，為盆地動力學研究打開了一個新窗口；研討了熱力構造研究方法。
  2. Conclusions : prokaryotic and eukaryocytic expression plasmids of the shortened hepatitis b surface antigen were successfully constracted, and the target proteins expressed by iptg induced in escherichia coli. as well as in eukaryocyte ( hepg2 and cos - 7 ), then their antigenity were detected

    結論：截短的乙型肝炎表面抗原分子的原核和真核表達』重組質粒成功被構建及分別在人腸桿菌efl得到誘導表達和存貞核細胞ifj表達，並檢測劍其表達產物的抗原特性。
  3. Marek " s disease virus ( mdv ) phosphoprotein 24 ( pp24 ) gene was amplified from md11 strain by polymerase chain reaction ( pcr ). then we cloned it into the downstream of gst gene according to the right open reading frame ( orf ) in pgex - 6p - l vector

    本研究將型mdvmd11株的pp24基因的完整orf克隆入原核表達載體pgex - 6p - 1中，重組質粒pgex - pp24轉化bl21宿主菌后，經iptg誘導表達。
  4. This paper is a study on the expression of the protective gene of bont / a in the prokaryotic and eukaryotic. it indicates the possibility to produce the recombinant protein in quantities. it has also laid down a good foundation for the further research on vaccine or antibody of bont / a

    本論文進行了人工合成的a型肉毒毒素hc段基因在原核和真核表達系統中的表達研究，使制備大量bont a保護型抗原的重組蛋白成為可能，為進一步進行a型肉毒毒素的疫苗或抗體研究奠定了一定的基礎。
  5. Thus, immunologists have sought smaller molecules with the antigen - recognition capability of antibodies. the variable domains of the heavy ( h ) and light ( l ) chains are sufficient for antigen recognition but the non - covalent complex of the two variable domains ( fv ) is unstable. it is possible to genetically engineer a single - chain fv ( scfv ) with the h chain v region connected to the l chain v region via a 15 amino acid linker composed of serine and glycine amino acid residues

    二、日本血吸蟲單克隆杭獨特型抗體np30的單鏈狐（ scf ）的構建、表達及對balbic小鼠誘導保護性作用研究l 、日本血吸蟲單克隆杭獨特型抗體np0的單鏈抗體歸cfv ）的構建、表達通過pcr方法體外擴增並經測序驗證的重鏈、輕鏈可變區（ vh 、 vl ）基因先後重組入原核表達質粒ptha90相應的位點上，中間通過一連接肽（ gly在er ） 。
  6. The e2 genes above of the prevalent strain ( guangxi yulin strain ) were cloned respectively into secreted expression vector ppic9k of eukaryotic expression system p. pastoris and transformed into p. pastoris by electroporation after linearization, 25 high - copied transformants were obtained by g418 screening. it was proved that the e2 genes were integrated stably into chromosome of p. pastoris by dot blot and dna sequencing

    豬瘟病毒e2基因的真核表達：分別將csfv兩個代表株的e2基因克隆入畢赤酵母（ p . pastoris ）分泌型表達載體ppic9k中，酶切線型化后電穿孔導入p . pastotis進行整合，經g418篩選得到25個高拷貝轉化子，經dna斑點試驗和dna測序證明外源基因e2穩定地整合到p . pastoris染色體中。
  7. Objective : to construct prokaryotic and eukaryocytic expression plasmids of the shortened hepatitis b surface antigen, and express the target proteins by iptg induced in escherichia coli

    目的：構建截短的乙型肝炎表面抗原分子的原核和真核表達重組質粒，然後分別在大腸桿菌中誘導表達並純化表達蛋白及在真核細胞中表達目的基因，並檢測其抗原特性。
  8. To prepare the wild type mbl in prokaryotic system, a pair of primers was designed and synthesized, and was used to amplify mbl gene from the recombinant vector pgem - mbl that contans wild type mbl cdna. a recombinant prokaryotic expression vector, pet28 - mbl, was constructed by inserted the mbl gene into plasmid pet28 ( b ), and after transfected it into ecoli bl21 ( de3 ) and induced with iptg, recombinant mbl protein was expressed successfully

    本實驗另選用了原核表達質粒pet28 （ b ） ，根據已構建好的含有mbl野生型基因的t載體pgem - mbl ，設計一對引物， pcr擴增mbl基因，凝膠回收，雙酶切pcr產物和pet28 （ b ）質粒， t4連接酶連接，轉化大腸桿菌dhsa ，氨芋選擇培養挑取克隆鑒定。
  9. They cannot naturally produce essential fatty acids - beneficial nutrients found mainly in plant and fish oil, so they must rely on a dietary supply. here we want to establish transgenic mice carry a tissue - specific expression gossypium hirsutum - 6 fatty acid desaturase ( fad2 ) gene which can add a double bond into an monosaturated fatty - acid hydrocarbon chain and convert oleic acid to linoleic

    本試驗試圖構建一個肌肉組織特性的- 6脂肪酸去飽和酶（脫氫酶）真核表達載體，通過原核顯微注射法把棉花的- 6脂肪酸去飽和酶導入到小鼠體內，以小鼠為動物模型，建立動物體內的必需脂肪酸合成代謝通路。
  10. In this article, the advanced structure of hybrid peptide mae is predicted with software. the fused gene mae - intein - cbd is amplified by pcr with the template of plasmid ptyb2, and then it is cloned into expression vector plasmid ppic9k. after verified by restriction enzyme analyzing and sequencing, the vector is transferred into the eukaryotic host ( yeast pichia

    並以已構建的載體ptyb2 - mae為模板，通過pcr擴增出融合基因mae - imein - cbd ，將其克隆于表達質粒ppic9k中，通過鑒定並測序正確后，電轉化真核表達宿主? ?畢赤酵母菌株gs115 ，通過營養缺陷型培養基篩選重組子，再利用g418抗性篩選出整合有多拷貝外源基因的重組子。
  11. Figure 2. dot plot of cd45 - side scatter ( ssc ) of leukemia bm cells. r1 is blast cells ; r2 is nucleated erythrocytes ; r3 is granulocytes ; r4 is lymphocytes

    有核紅細胞和原始細胞群，對不同的細胞群體框定（即射門）后，即可知各群細胞所佔的比例，並可明確顯示幼稚細胞群體的免疫表型（圖2 ） 。
  12. In this article, the misgurin gene and adaptor are synthesized according to the amino acid sequence reported in the genebank and the need of construction and expression. adopted a new strategy, multiple copy gene is ligated in the same direction. and then it is cloned into expression vector plasmid ppic9k

    本文根據genebank登錄的氨基酸序列，同時考慮構建和表達的需要，化學合成了misgurin基因和接頭，採用一種新的策略，在體外將基因多拷貝同向串連，並將其克隆于表達質粒ppic9k中，通過鑒定並測序正確后，電轉化真核表達宿主? ?畢赤酵母菌株gs115 ，通過營養缺陷型培養基篩選重組子，再利用g418抗性篩選出整合有多拷貝外源基因的重組子。
  13. An example shows that the core models are consistent with the characteristics of chinese urban roads and the efficiency of plane road intersection design can be improved greatly

    應用實例表明該系統內核模型符合我國道路交通的實際情況，能大大提高道路平面交叉口設計的效率。
  14. We have created transgenic tobacco plants in which expression of the atnced3 coding region is under strong constitutive 35s cauliflower mosaic virus ( 35s ) promoter, stress - inducible rd29a promoter or stomotal - specific kst1 promoter by agrobacterium tumefaciens - mediated transformation

    構建了組成型35s 、誘導型rd29a 、氣孔特異表達性kst1啟動子驅動atnced _ 3基因表達的真核表達載體，通過農桿菌介導的葉盤法轉化煙草，得到了轉基因植株。
  15. To construct eukaryotic expression vector of mbl gene with codon 54 point mutation, the target sequence in pgem - mbld plasmid, which conains mbl cdna with codon 54 mutant allele, was amplified by pcr. after the cdna fragement and plasmids pci - neo were prepared by digestion with sma i and sal i, the fragment was inserted into sma i and sal i site in pci - neo eukaryotic expression vector, and the recombinant vector, named pci - mbl54, was obtained. the pci - mbl54, digested with restriction enzymes, was found to contain the point mutation mbl cdna by agarose gel electrophoresis analysis

    本實驗以ggc54gacmbl突變為研究對象，選用真核表達質粒pci - neo ，根據已構建好的含54位密碼子突變型mbl基因t載體的結構，設計合成新的引物， pcr擴增54位密碼突變型mbl基因，凝膠回收，雙酶切pcr產物和pci - neo質粒， t4連接酶連接，將前者克隆至後者的sma和sal位點，轉化大腸桿菌xl - 1blue ，氨芐選擇培養。
  16. By gene fusion and prokaryotic expression, we purified a pea actin isoform ( peac1 ), his - tagged peac1, his - tagged gfp and his - tagged peac1 - gfp from inclusion body. after filtrating a series of induction condition, we expressed and purified his - tagged peac1 with soluble form in a large amount

    利用基因融合技術，原核表達並從包涵體中純化了豌豆肌動蛋白異型體peac1 、 his - taggedpeac1 、 his - taggedgfp以及his - taggedpeac1 - gfp ，並通過誘導條件的篩選達到了可溶性表達與大量純化his - taggedpeac1 - gfp的目的。
  17. The results of confocal laser scanning microscopy ( clsm ) and atomic force microscopy ( afm ) proved that the microspheres or nanospheres with different surface characters were obtained successfully. the clsm investigation of fitc labeled particles showed that polymeric microspheres have a core - shell structure in which the surfactants existed in the shell of particles. polymeric microspheres with different surface characters were embedded onto the surface of pla membranes via surface entrapment

    激光共聚焦顯微鏡（ clsm ）和原子力顯微鏡（ afm ）觀察分析結果證實可通過上述技術獲得具有不同表面性質的微米級或納米級聚合物微球，而熒光標記技術（ fitc ）則證實了聚合物微球具有明顯的核殼型結構，表面穩定劑存在於微球的殼層。
  18. The mechanism is that the introduced complementary oligonucleotides can bind to the corresponding mrna or double - stranded dna in genome and form partial double - stranded molecules or triple - stranded nucleic acid molecules by sequence - specific and nonsequence - specific antisense action, thus the target gene will be orientationally blocked and expression of the target inhibited so that therapeutic effect could be attained. in this study, we designed a fragment of human c ii ta cdna in antisense orientation using mrna of c ii ta as template. the primers were designed based on 94 - 500 nucleotides segment in 5 " end of ciita gene so that the interested gene contained 407 base pairs which included two aug codons in 1 16 and 188 nucleotides as well as the splicing site between the first and the second exons

    本研究設計以c tamrna為模板的反義cdna片段，從c ta基因5 』端第94位到500位核苷酸段設計引物，目的片段407bp ，覆蓋第116和188位兩個aug密碼子，也包含了第一外顯子和第二外顯子間的剪接位點：用常規分子生物學方法構建了反義片段的腺病毒表達載體（ padeasy - 1系統） ；腺病毒載體經hek293細胞包裝產生含反義片段的重組腺病毒，用氯化銫密度梯度離心法獲得純化的高滴度腺病毒；進行體外基因轉移，分別用反義片段真核表達載體轉染p388d1細胞和用重組腺病毒感染hela細胞，觀察導入的c ta基因反義rna抑制細胞內組成型或誘導型c ta基因表達的作用，從而達到調控mhc -類分子表達的目的。
  19. In the first part, we observed the changes of expressions of type i receptor of il - 1 in the rat and mouse brain after intraperitoneally administration of different kinds and doses antigens respectively. in the second part including two experiments we cloned rat il - 6r ' s genes by pcr, expressed them in e. coli dh5 a and cos - 7 cell, and produced il - 6r ' s polyclonal antibody which is proved having more high liter, affinity and specificity 1

    第一部分採用不同種類和劑量的抗原刺激，分兩個實驗，觀察了大、小鼠腦內il - 1的i型受體表達的變化，探討了腦內參與免疫調控的核團和細胞類型；第二部分分兩個實驗，運用pcr技術克隆了大鼠il - 6r的基因並進行了原核和真核表達，制備了特異性高、親和力強和較高效價的抗il - 6r多克隆抗體，為進一步進行il - 6r的研究奠定了基礎。
  20. Our research area focus on exploring the course and regulation mechanism of the proliferation and differentiation and apoptosis of eukaryocyte, and studying the cellular canceration and the practicable way to control the malignant phentype of carcinoma cells

    探索真核細胞增殖分化與凋亡過程及其調控機理，研究細胞癌變和控制癌細胞惡性表型的可行途徑
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