核誘導法 的英文怎麼說

中文拼音 [héyòudǎofǎ]
核誘導法 英文
nuclear induction method
  • 核 : 核構詞成分。
  • 誘 : 動詞1. （誘導） guide; lead; induce 2. （使用手段引人隨從自己的意願） lure; seduce; entice
  • 導 : 動詞1. （引導） lead; guide 2. （傳導） transmit; conduct 3. （開導） instruct; teach; give guidance to
  • 法 : Ⅰ名詞1 （由國家制定或認可的行為規則的總稱） law 2 （方法; 方式） way; method; mode; means 3 （標...
  • 誘導 : guide; lead; induce; guidance; induction
  1. Within 2h isolation from the follicle 100 % of oocytes underwent germinal vesicle breakdown and both the nucleolus and the nuclear envelope disappeared. after culturing for 6 h, prometaphase occurred in 90 % oocytes

    本研究旨在探索一種嶄新的、化學試劑誘導去核卵母細胞為核受體的、無透明帶的、手工體細胞核移植方法。
  2. To add " there are many loopholes in the operation and sales practices of telecommunications service providers, " after " that, as " ; to add " and such complaints from the public are often not followed up effectively by government departments, " after " in recent years, " ; to add " stipulate corresponding penalties " after " to cover pay television, " ; and to add " ; furthermore, as the hong kong housing authority has signed agreements with pay television operators, these operators can enter the buildings to set up and maintain communal aerial systems and can deploy front - line promoters to approach the residents to sell other various commercial services, using the provision of exclusive maintenance and supply services as a selling point and adopting unscrupulous means to entice or mislead the residents into signing an agreement with them, causing great disturbance to the residents ; in this connection, the government must adopt measures to combat such sales malpractices, take the initiative to inform public rental housing tenants of the rights and responsibilities of the operators and, when the operators resort to malpractices or malpractices are reported by residents, take immediate action to investigate the matter and issue warning to and penalize the operators concerned ; the government must also review the problem of inequity in accessing system information by consumers and the telecommunications service providers, whereby consumers have no means or right to obtain true and accurate information about the systems that they are using and can only pay the fees according to the volume, system and time slots of their calls shown on the records provided unilaterally by the service providers, and in case of queries about such information, the decision of the service providers prevails and there is no channel for the consumers to dispute or verify ; to this end, the government should expeditiously study how to ensure that consumers have the rights to know, choose, verify, appeal and claim for compensation in the provision of telecommunications services including pay television, local and cross - boundary telecommunications and internet services " after " in the contracts "

    在"鑒于"之後加上"各電訊服務提供者的營運和銷售存在不少漏洞， "在"不斷增加， "之後加上"而市民的投訴又往往得不到政府部門的有效跟進處理， "在"收費電視， "之後加上"訂定相應的罰則， "及在"標準合約條款"之後加上"此外，由於香港房屋委員會與收費電視營辦商簽訂協議，該等營辦商可進入大廈鋪設及維修公共天線系統，並調派前線銷售人員，以獨家提供維修及供應服務作為招徠，向住戶推銷其他各種商業服務，以不當手法誘使或誤導住戶與他們簽約，對居民造成很大困擾就此，政府必須採取措施打擊上述違規營銷的手法，並主動告知公屋住戶該等營辦商的權責范圍，遇有營辦商作出違規的行為或居民舉報營辦商的違規行為時，須立即追查，並向有關營辦商發出警告及作出處分政府亦須檢討現時消費者與電訊服務供應商所獲系統資訊並不對等的問題，即消費者無從及無權得知他們所選用系統的真實資料，令他們只能按服務供應商單方面提供有關通話量通話系統及通話時段的記錄繳交費用，遇有消費者質疑上述資料時，往往只由服務供應商作最終決定，消費者無從申辯或查證就此，本會促請政府盡快研究如何就各類電訊服務包括收費電視本地及跨境電訊網際網路等的提供，確保消費者享有知情權選擇權覆核權投訴權及索償權" 。
  3. Thus, immunologists have sought smaller molecules with the antigen - recognition capability of antibodies. the variable domains of the heavy ( h ) and light ( l ) chains are sufficient for antigen recognition but the non - covalent complex of the two variable domains ( fv ) is unstable. it is possible to genetically engineer a single - chain fv ( scfv ) with the h chain v region connected to the l chain v region via a 15 amino acid linker composed of serine and glycine amino acid residues

    二、日本血吸蟲單克隆杭獨特型抗體np30的單鏈狐（ scf ）的構建、表達及對balbic小鼠誘導保護性作用研究l 、日本血吸蟲單克隆杭獨特型抗體np0的單鏈抗體歸cfv ）的構建、表達通過pcr方法體外擴增並經測序驗證的重鏈、輕鏈可變區（ vh 、 vl ）基因先後重組入原核表達質粒ptha90相應的位點上，中間通過一連接肽（ gly在er ） 。
  4. Methods serums containing whole wmt and its disassembled formulas, including the formula consisted of warming jing and boosting qi part wenjin yiqi, wy and that of promoting blood circulation part huoxue tongmai, ht, as well as the serum contained high concentration of lipids were prepared conventionally, respectively. the adhesion of monocytes cell strain thp1 to human umbilical vascular endothelial cells huvec was determined by rose bengal stain method, and elisa was used to detect expressions of intercellular adhesion molecule icam1, vascular cellular adhesion molecule vcam1 and p selectin on huvec surface

    方法常規制備溫脈通全方溫經益氣拆方活血通脈拆方含藥血清和高脂血清，採用虎紅染色法檢測藥物血清對高脂誘導的人臍靜脈內皮細胞huvec和單核細胞株thp1黏附的作用用細胞elisa法檢測huvec表面細胞間黏附分子1 icam1血管細胞黏附分子1 vcam1 p選擇素pselectin的表達。
  5. Salmonella typhimuriwn, one of the invasive bacterial species, can be attenuated without loss of invasiveness and thus used for delivery of eukaryotic expression vectors into host cells in vivo. the recombinant plasmid containing the target gene is released inside the host cells and gain entry into the nucleus, resulting in expression of encoded antigens and subsequent induction of humoral and cellular immune responses

    沙門氏菌（ salmonellatyphimurium ）是一種較為常見的侵襲性胞內菌，通過基因工程方法減毒后對宿主致病性顯著降低，但仍保留良好的侵襲力，可直接將真核表達質粒攜帶進入動物細胞內表達相應的蛋白而誘導特異性的免疫應答反應。
  6. The high - enzyme activity has 2 base changes, resulting in long amino acid sequence with native amylase. this inducing method resolved the problem of non - effective induction as in base analogue induction. and the method we used provide a new measure for this kind of work

    高酶活編碼區位點突變導致c -端序列變化和終止子的后移本誘變方法克服了用堿基類似物在體內誘變由於核酸復制酶等的校正作用而造成誘變無效的難題，為基因的誘變找到了一條新途經。
  7. At the same time, parking difficulty bring out out - of - order parking, traffic confusion, traffic accidents, environment pollution and etc. therefore, parking problem has been pay attention to by the related management department of government and drivers. faced with the status of insufficient urban road resources, the experts and scholars of transportation engineering proposed its ( intelligent transportation systems ) to settle traffic problems and improve transportation efficiency. pgs ( parking guidance system ) is the application of its in the parking field, and the efficient approach to enhance urban parking management level and settle urban parking difficulty

    本論文依託吉林省科技廳科技發展計劃項目《停車誘導系統理論方法和實施技術研究》 ，以停車誘導系統的核心? ?停車信息為主線，主要剖析如下五個問題:為什麼要進行有效停車泊位預測及預測的方法;如何基於停車場選擇進行停車誘導路徑優化;怎樣實現停車泊位預定;如何合理發布停車泊位信息;通過什麼方法來實現停車泊位信息的高效傳輸。
  8. In order to further investigate the role of axudl in human tumor carcinogenesis and the potential association between the axudl gene expression status and the stimulation of transforming growth factor beta in human cancers, the present study was performed in three aspects as follows : ( 1 ) cloning full length enconding region cdna of axudl and construction of eukaryotic vector that expression the fusion protein of axud1 and influenza virus hemagglutin ha epitope tag ; ( 2 ) exploring the time and dose effects of tgf - 1 on the expression - of axudl gene in hepg2 hepatoma cells and spc - a1 lung carcinomas cells, and studying the effects of overexpression of axud1 on the expression of cell cycle and apoptosis related protein in hepg2 hepatoma cells ; ( 3 ) construction and expression of human axudl in e. coli m15. the following main results and conclusions can be obtained from the present study : 1. the full length ecnoding region of human axudl cdna from human peripheral blood lymphocytes was successfully cloned using one step rt - pcr method, and constructed into a eukaryotic expression vector which can be expressed a ha - axud1 fusion protein with axud1 and influenza virus hemagglutin ha epitope tag. the recombinant plasmid was identified by polymerase chain reaction, restriction endonuclease maping and sequencing, this expression vector might be instrumental to further study the function of axud1 protein in tumor cells

    為了進一步研究axud1在人類腫瘤發生中的作用及axud1基因的表達狀況與tgf -介導的信號通路的關系，本實驗研究分為三個部分： （ 1 ） axud1基因cdna全長編碼區的克隆和ha表位標記的axud1基因表達載體的構建； （ 2 ）探討肝癌細胞hepg2和肺腺癌spc - a1細胞中tgf - 1誘導的axud1基因表達的時間、劑量效應以及誘導表達的可能機理，並研究axud1的過表達對細胞周期和細胞凋亡相關蛋白表達的影響； （ 3 ） axud1原核表達載體的構建及其在大腸桿菌中的表達。本實驗的主要結果和結論如下： 1利用一步法rt - pcr成功地從人類外周血淋巴細胞中克隆出axud1基因編碼區cdna ，並將其構建入真核表達載體中，編碼的ha - axud1融合蛋白帶有流感病毒凝血素ha的表位標記肽段。
  9. We have created transgenic tobacco plants in which expression of the atnced3 coding region is under strong constitutive 35s cauliflower mosaic virus ( 35s ) promoter, stress - inducible rd29a promoter or stomotal - specific kst1 promoter by agrobacterium tumefaciens - mediated transformation

    構建了組成型35s 、誘導型rd29a 、氣孔特異表達性kst1啟動子驅動atnced _ 3基因表達的真核表達載體，通過農桿菌介導的葉盤法轉化煙草，得到了轉基因植株。
  10. Under such accounting checking method, more and more stern social contradictory, such as the question of population, resources, environment and the public causes are exposed. under this circumstance, we have to exam the social part of enterprises again. enterprises are required to burden corresponding social responsibilities during the course of running for high gains, acquiring cooperated development between enterprise and society and carry out the strategy of sustainable development

    傳統的會計核算模式是將企業作為一個獨立和封閉的經濟實體，對其生產經營情況進行核算，這一核算方法主導下的生產經營模式已經誘導了日益嚴重的社會問題，在這一背景下，人們不得不重新審視企業的社會角色，要求企業在追求自身高利潤率的同時也要切實地承擔相應的社會責任，實現企業和社會的協調發展，實施可持續發展戰略。
  11. Purification of the recombinant expressed endostatin in this work we have successfully cloned mouse endostatin gene, and constructed pbv220 - endostatin expression plasmid that is a non - fusion expression vector. the positive pbv220 - endostatin was transformed into dh5a, and expressed mouse endostatin protein successfully. this study is the basis of the endostatin gene - engineering production

    本文成功克隆鼠內皮抑素基因，構建了內皮抑素基因的原核表達載體pbv220一endostatin ，該載體為非融合性表達載體，將pbv22o一endostatin成功轉化到大腸桿菌表達菌dh5a中，並誘導表達了鼠內皮抑素蛋白，為基因工程方法生產內皮抑素奠定一定基礎。
  12. The mechanism is that the introduced complementary oligonucleotides can bind to the corresponding mrna or double - stranded dna in genome and form partial double - stranded molecules or triple - stranded nucleic acid molecules by sequence - specific and nonsequence - specific antisense action, thus the target gene will be orientationally blocked and expression of the target inhibited so that therapeutic effect could be attained. in this study, we designed a fragment of human c ii ta cdna in antisense orientation using mrna of c ii ta as template. the primers were designed based on 94 - 500 nucleotides segment in 5 " end of ciita gene so that the interested gene contained 407 base pairs which included two aug codons in 1 16 and 188 nucleotides as well as the splicing site between the first and the second exons

    本研究設計以c tamrna為模板的反義cdna片段，從c ta基因5 』端第94位到500位核苷酸段設計引物，目的片段407bp ，覆蓋第116和188位兩個aug密碼子，也包含了第一外顯子和第二外顯子間的剪接位點：用常規分子生物學方法構建了反義片段的腺病毒表達載體（ padeasy - 1系統） ；腺病毒載體經hek293細胞包裝產生含反義片段的重組腺病毒，用氯化銫密度梯度離心法獲得純化的高滴度腺病毒；進行體外基因轉移，分別用反義片段真核表達載體轉染p388d1細胞和用重組腺病毒感染hela細胞，觀察導入的c ta基因反義rna抑制細胞內組成型或誘導型c ta基因表達的作用，從而達到調控mhc -類分子表達的目的。
  13. Furthermore, suppression subtractive hybridization ( ssh ) was employed for the isolation of cdna fragments for euonymus japonicus " zhuzi " differentially expressed genes, and forward suppression subtractive cdna library of cold - regulated genes was constructed. the seedlings of euonymus japonicus " zhuzi " treated with low temperature were as tester and untreated seedlings as driver. subtractive cdna library was differentially screened through cdna macroarray, six hundreds and four cdna clones were identified as cold specifically induced or highly expressed

    （ 5 ）應用抑制差減雜交（ suppressionsubtractivehybridization ， ssh ）方法，構建冷誘導表達的正向抑制差減cdna文庫，低溫處理的幼苗為tester ，常溫處理為driver ，通過cdna微陣列差異篩選cdna文庫，得到604個低溫誘導或表達增強的候選克隆，對其中的84克隆進行dna測序，去除冗餘的cdna ，在genbank中進行核酸和蛋白質同源性的比較和功能分析，共有36個單一序列，其中12個cdna在genbank數據庫沒有同源的序列。
  14. Methods : the mouse pem gene ( mpem ) cdna coding sequence was cloned into prokaryotic gst fusion protein expression plasmid pgex - 4t - 3. the recombinant plasmid was transformed to e. coli bl21 and the gst / mpem fusion protein was induced to express with iptg. the fusion protein was purified by affinity chromatography

    方法： pcr擴增小鼠pemcdna編碼序列，將它克隆到含有gst的原核表達質粒載體pgex - 4t - 3上，轉化大腸桿菌bl21 ，誘導表達gst mpem融合蛋白，通過親和層析，獲得初步純化的產物，以羥胺切割驗證其一級結構。
  15. The method of numeral simulating the coincidence neutron detection is proposed with combining the neutron transportation and probabilistic calculation. the method is introduced and used to simulate the nuclear warhead induced fission by source neutrons with energy below 1 mev

    推導並提出用中子輸運計算與概率方法結合計算符合中子計數，對能量低於1mev的外源中子照射核彈頭誘發裂變的符合中子測量作了數值模擬。
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