核酸性蛋白 的英文怎麼說
中文拼音 [hésuānxìngdànbái]
核酸性蛋白
英文
nuclear acidic protein- 核 : 核構詞成分。
- 酸 : 酸構詞成分。
- 性 : Ⅰ名詞1 (性格) nature; character; disposition 2 (性能; 性質) property; quality 3 (性別) sex ...
- 蛋 : 名詞1. (鳥類或龜、蛇類所產的卵) egg 2. (像蛋形的東西) an egg-shaped thing 3. (辱罵之詞)
- 白 : Ⅰ形容詞1 (似雪的顏色) white 2 (清楚; 明白; 弄明白) clear 3 (空的; 沒加他物的) pure; clear; ...
- 酸性 : [化學] acidity; acidness; acerbic; acidic property酸性材料 acid material; 酸性促進劑 acid acceler...
- 蛋白 : 1. (卵中透明的膠狀物質) egg white; albumen; gary2. [生物化學] (蛋白質) protein
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At rest, the heterodimeric rel / nf - k b complex is located in the cytoplasm bound to an inhibitory factor, i k b. upon stimulation, i k b is phosphorylated and degraded, free nf - k b then translocates into the nucleus where it binds to k b site to regulate transcription
靜息時, re皿4bh聚體與抑制蛋白kbs結合,在細胞漿中保持無活性。受到刺激, ikb分子迅速磷酸化並降解,釋放nf兒b轉位入核,通過與kb位點結合調節轉錄。The inclusion bodies of recombinant protein were purified with washing buffer consisting of various urea ( 2mol / l and 4mol / l ) for several times, and then dissolve the fusion protein in the denature buffer using 8mol / l urea as denaturant
用含有2mol l和4mol l尿素的包涵體洗滌液洗滌包涵體,在37條件下,洗去了大部分菌體蛋白及其它核酸物質。用8mol l尿素作為變性劑溶解包涵體,包涵體在8mol l尿素中的溶解性非常好。Whole mount in situ hybridization showed that amphihmgb transcripts were present ubiquitously in the early embryos from zygote to neurula, but they decreased gradually in mesoderm and endoderm and neural tube with development, and became restricted to epidermal cells in the hatched larva. it appear s that hmgb plays a role in the mitosis and differentiation of embryonic cells. another amphioxus cdna, amphiubf80, encoding ubiquitin / ribosomal fused protein s27a, was isolated from the gut cdna library of b. belcheri tsingtauense
S004cdna序列編碼一個156個氨基酸殘基組成的蛋白質,與人的泛素蛋白ubf80具有較高的氨基酸序列同源性( 145 / 156同源) ,且該蛋白具有泛素蛋白ubf80所共有的兩個結構域,即由72個氨基酸組成的泛素介導的蛋白降解結構域和由48個氨基酸組成的核糖體蛋白s27a結構域。Cyclic nucleotide dependent protein kinases
環化核苷酸依賴性蛋白質激酶G gene of rabies virus m, the of two main regions ( about 1000nt ), ranging from 3161nt to 4162nt and ranging from 4012nt to 4863nt of glycoprotein gene of rabies virus strain m, isolated from mouse in he nan, china were amplified by reverse transcriptase - polynerase chain reaction ( rt - pcr ) in order to complete glycoprotein gene of strain m. these regions were sequenced by the produce of pcr directly. comparison and analysis of nucleotide sequence and amino acid sequence deduced with that of other strains published was performed by computer with dnasisv 2. 5demo software
本研究對我國河南某地野鼠體內分離的狂犬病野毒株mrv基因的3161位? 4162位( 1001個堿基)和4012位? 4863位( 851個堿基)片段進行了反轉錄pcr擴增和序列測定,得到mrv的糖蛋白基因全序列,用dnasisv2 . 5demo分析軟體,與已發表的代表性毒株g基因全序列進行核苷酸和氨基酸序列的比較分析,結果表明在同一基因型中, mrv和國際標準攻毒株cvs的同源性最高( 96 . 5 ) ,和中國減毒株ctn的同源性最低( 79 . 8 ) 。Tsarg2 with 1 233 bp length was composed of 6 exons and spaned about 115 kb of genomic dna, the putative protein encoded by this gene was 305 amino acid with a theoretical mass of 34 751 and with no significant homology with any known protein in databases. a kind of nucleoprotein was the most impossible
Tsarg2基因的cdna全長為1233bp ,包含6個外顯子,基因組跨越115kb ,編碼由305個氨基酸組成的、分子量為34751的蛋白質,與已知蛋白質無明顯同源性,其最大可能是一種核蛋白。With the increase of concentration of aqueous extract from peganum multisectum increased, root vigor, the contents of chlorophyll, soluble protein and nucleic acid in roots and shoots of alfalfa seedlings decreased, while the activities of protease and nuclease, the contents of o2 ( superscript - ), h2o2 and malondiadehyde ( mda ) increased, the activities of superoxide dismutase ( sod ), catalase ( cat ) and peroxidase ( pod ) first increased and then decreased
幼苗根系活力和葉綠素、可溶性蛋白質、核酸含量隨水浸液濃度的提高而降低,蛋白酶和核糖核酸酶活性及超氧陰離子( o2 (上標- ) ) 、 h2o2和丙二醛( mda )含量則增加,超氧化物歧化酶( sod ) 、過氧化氫酶( cat )和過氧化物酶( pod )活性呈先升后降變化。Ros damage nucleic acid, protein and membrane
活性氧對核酸、蛋白、膜造成傷害。Methods : hyperosmotic pressure animal model was established by administering 3 % sodium chloride as drinking water to rats or increasing osmotic pressure of the culture medium. osmoregulation positions in the brain, reciprocal projection pathways between the medullary visceral zone ( mvz ) and supraoptic nucleus ( son ) or hypothalamic paraventricular nucleus ( pvn ), oscillation of intracellular calcium in cultured neurons and astrocytes were studied by means of anti - fos, glial fibrillary acidic protein ( gfap ), tyrosine hydroxylase ( th ) or vasopressin ( vp ) multiple imrnunohistochemical staining, immuno - electronic microscope, wga - hrp retrogradely tracing and cell culture methods. results : ( 1 ) fos positive neurons within the mvz, parabrachial nuclei, locus ceruleus, pvn, son, subfomical organ increased markedly
方法:通過給予大鼠飲用3氯化鈉或提高培養基滲透壓濃度的方法復制高滲刺激模型,主要採用抗fos 、膠質原纖維酸性蛋白( gfap )和酪氨酸羥化酶( th ) (或加壓素? vp )免疫組織化學多重染色、免疫電鏡、 wga - hrp束路追蹤結合免疫組織化學多重染色、細胞培養等實驗方法,系統觀察了中樞參與滲透壓反射的調控部位、下丘腦視上核( son )神經元? ast超微結構的變化、延髓內臟帶( mvz )和son及下丘腦室旁核( pvn )之間往返投射通路和神經元的性質及其與ast的關系、培養神經元和ast內鈣波的變化。Results show that vp37 protein can bind single strand nucleic acid cooperatively and nonspecificallty, and the vp37 - ssrna complex was stable at high salt concentrations, suggesting vp37 is a possible mp. vp37 is the only protein characterized so far showing rna - binding ability in genus fabavirus
為了驗證vp37是否具有核酸結合能力,我們利用6his - vp37蛋白進行了核酸結合實驗,結果表明vp37是一種能非特異性結合單鏈核酸的蛋白;其在結合核酸時具有協同性; na ~ +的變化對其核酸結合能力影響較小。Histidine a basic amino acid formed only rarely in proteins. it is formed from atp and 5 - phosphoribosyl pyrophosphate in a complex biosynthetic pathway. glutamate is an intermediate in its breakdown
組氨酸:是蛋白質中少有的一種堿性氨基酸。由atp和5 -磷酸核糖基焦磷酸鹽通過復雜的生物合成途徑合成。在其降解過程中可形成谷氨酸。The cloning cdna fragment was extracted from positive clones and sequenced. the results showed that the cdna fragment was 816bp in size, encoding a protein which included 272 amino acids. the sequence homology analysis was carried out via the software blast 2. 0 network service in the four large databases - genbank, embl, ddbj, pdb, which had recorded 1 337 978 nucleotide and protein sequences. the results of the analysis indicated that the nucleotide homologous rates between the rubber tree etr and 15 recorded etrl of other plants ( mango, passion fruit, persia plum, strawberry, grape. . etc ) were 75 % - 80 % ; the protein homologous rates between the rubber tree etrl and these recorded etrl genes were 90 % - 95 %. from the results mentioned above, we could confirm that the cdna of rubber tree etrl had been cloned
從陽性克隆子中提取克隆片段,經序列測定分析,結果表明,克隆片段的cdna大小為816bp ,編碼的蛋白質包含272個氨基酸。基因序列通過blast2 . 0networkservice軟體對genbank , embl , ddbj , pdb四個大型數據庫中記錄的1337978條核酸和蛋白質序列進行序列相似性檢索,結果表明與芒果、一西番蓮、波斯梅、草毒、葡萄、西洋梨等15種已報道的植物的etrl基因cdnag的同源率為75 88 ;蛋白質氨基酸序列的同源率為90 95 ,表明本研究確實克隆到了橡膠樹etri基因的cdna序列。 4It is a very basic, single - chain protein with molecular weight about 14 kd. it shares 33 % sequence identity with bovine pancreatic rnasea and has structurally equivalent counterparts for the two histidines and one lysine that comprise the catalytic residues for ribonucleolytic activity
它是由123個氨基酸組成的分子量約為14kd的單鏈堿性蛋白質,與牛胰核糖核酸酶有33的同源性,兩個組氨酸和一個賴氨酸殘基組成了血管生成素核糖核酸酶活性中心。In this study we firstly determined the time when the chitinase of helicoverpa armlerra produced in high activity and we cloned a cdna encoding a chitinase of helicoverpa armlerra by race method which was about l. 6kb and included complete 5 ' - end cap and 5 ' - utr
利用計算機分析該核昔酸序列,可知其編碼框為1341個核昔酸,編碼50kd含446個氨基酸的蛋白,推導等電點為phi9 575 ,為堿性蛋白。Change of immunosuppressive acidic protein in sera of patients with pulmonary tuberculosis and its clinical significance
肺結核患者血清中免疫抑制酸性蛋白變化及其臨床意義Tissue sections from every animal were double - labeled with the antibodies of map - 2, cox - 2, gdnf, caspase - 3 and either the neuron - specific antibody neuronal nuclear protein ( neun ) or the astroglial - specific marker glial fibrillary acidic protein ( gfap ). we carried out a series of research to explore the effects and mechanism of map - 2, cox - 2, gdnf, caspase - 3 during tbi and trie d to provide some useful theory basis for both the treatment of tbi in the practice and forensic medicine
並通過上述指標分別與神經元特異性標志物神經元核蛋白( neuronalnuclearprotein , neun )和星形膠質細胞特異標志物膠質纖維酸性蛋白( glialfibrillaryacidicprotein , gfap )進行免疫組織化學雙染色,探討腦損傷后神經元及神經膠質細胞反應性變化情況及其分子生物學機制,以期為腦損傷研究提供有益的數據材料,也為以上指標在法醫學實際檢案的應用提供必需理論依據。Quantify the relevance hydrophobicity in protein structure : the general expectation is that hydrophobic aminoacids are in the core of proteins, while polar aminoacids are on the surface
量化蛋白質結構的參比疏水性:一般認為,疏水性氨基酸在蛋白質的核中,而極性氨基酸在表面。It possess high homology with those of ck2 ( casein kinase 2 ) a subunits from various species. it is now well established that protein kinase ck2 is a pleiotropic and abiquitous serine or threonine kinase, which is highly conserved during evolution. we obtained the complete cdna sequence of ck2 a via 5 " race
酪蛋白激酶ck2是目前發現的普遍存在於真核生物中的絲氨酸蘇氨酸型蛋白激酶,進化上具有高度的保守性並參與了多種生理功能的調控,因而克隆它的基因具有重要意義。The cdna expression library that contains no intron theoretically include all expressed genes and conserve resource genes permanently. lt can be used to find out and clone some genes which can express particular protein with modern molecular biological techniques, such as immunological screening, drug - prob screening, southern et al. lt is very important to study the life nature of plasmodium falciparum in molecular level. with the developments of these studies, the drug - resistant mechanism of the plasmodium falciparum and the genes of some specific medicine binding protein can be made well - known. at the same time, the researches will do good to explain the mechanism of some specific medicines in order to design and screen new anti - malaria drugs
建立cdna表達文庫在一次永久保存基因資源的同時,可以利用功能篩選、免疫學篩選、藥物探針篩選、 southern雜交和大規模序列測定等現代分子生物學技術尋找特異性活性蛋白基因,進而克隆和表達這些基因,對從核酸及蛋白質等分子水平研究瘧原蟲的生命活動規律,對揭示其抗藥性分子機理,搞清某些特效藥物結合蛋白的基因及此類藥物的作用機制,對新型抗瘧藥物的合理設計及篩選都具有極其重要的現實意義。Furthermore, suppression subtractive hybridization ( ssh ) was employed for the isolation of cdna fragments for euonymus japonicus " zhuzi " differentially expressed genes, and forward suppression subtractive cdna library of cold - regulated genes was constructed. the seedlings of euonymus japonicus " zhuzi " treated with low temperature were as tester and untreated seedlings as driver. subtractive cdna library was differentially screened through cdna macroarray, six hundreds and four cdna clones were identified as cold specifically induced or highly expressed
( 5 )應用抑制差減雜交( suppressionsubtractivehybridization , ssh )方法,構建冷誘導表達的正向抑制差減cdna文庫,低溫處理的幼苗為tester ,常溫處理為driver ,通過cdna微陣列差異篩選cdna文庫,得到604個低溫誘導或表達增強的候選克隆,對其中的84克隆進行dna測序,去除冗餘的cdna ,在genbank中進行核酸和蛋白質同源性的比較和功能分析,共有36個單一序列,其中12個cdna在genbank數據庫沒有同源的序列。分享友人