根瘤桿菌 的英文怎麼說
中文拼音 [gēnliúgǎnjūn]
根瘤桿菌
英文
bacillus radicicola-
In addition, it was also found that this protein shares limited but significant homology with the sam - dependent methyltransferase of mesorhizobium sp. bnc1 ( 32 % similarity ), and the similarity of its 303 - 362 region to the 160 - 220 domains of l11 methyltransferases of e. coli ( prma ) is 41 %. it is suggested that methylation of l11 resulted in effects of noea on nodulation of 042bm
發現noea與中慢生根瘤菌（ mesorhizobiumsp . ） bnc1可能的sam -依賴性的甲基轉移酶相似性為32 ，而其303 - 362區域與大腸桿菌（ escherichiacoli ）的核糖體50s亞基的l11蛋白甲基轉移酶（ prma ）的160 - 220結構域的相似性達到41 。Identification of functional bacteria showed predominant ammonifiers were shewanella, variovorax, chryseobacterium, bacillus or aeromonas ; among 4 selected nitrogen fixers, one ( azorhizobium caulinodans ) belonged to. a - proteobacteria, the other three ( serratia marcescens, klebsiella pneumoniae and citrobacter freundii ) were enterobacteriace, which belongs to - proteobacteria ; 2 nitrate reducers were aeromonas sp. and citrobacter sp.,
對各功能菌群中的優勢菌的鑒定表明，優勢的氨化細菌為希瓦氏菌屬，產堿菌屬，黃桿菌屬，芽孢桿菌屬或氣單胞菌屬；分離到的4個優勢固氮細菌菌株中，一株為基瘤固氮根瘤菌，屬于-變形菌亞門，而另外3株都屬于腸桿菌科，歸于-變形菌亞門。The suitable concentration of hyg to screen for resistant shoots was 50 mg / l. the kind of antibiotics to inhibit the growth of agrobacterium eha105 was cef, of which concentration was 250 mg / l
以洋桔梗葉盤為外植體，通過根瘤農桿菌菌株eha105介導，用構建於質粒pcambia1301的npr1基因轉化洋桔梗。Agrobacterium a genus of soil bacteria, the species a. tumefaciens being the causative agent of crown gall, a type of tumor in plants
根癌農桿菌：土壤中存在的一種農桿菌，可以感染雙子葉植物的受傷組織誘發一種植物的腫瘤冠癭瘤。Ssmapkk transformations were screened on media with kanamycin ( 30mg / l ). nineteen individual kanamycin resistant plants were obtained. t2 plants were checked for integration of foreign gene by counting ratio of the number of tolerant plants to the number of non - tolerant plants on selection medium with kanamycin ( 30mg / l )
將ssvp和ssmapkk的全長cdna分別克隆入植物表達載體pcambia1300和prok中，導入根瘤農桿菌gv3101后，由花浸泡法進行擬南芥遺傳轉化，轉化ssvp鹽地堿蓬ssop和ssmapkk基因的克隆與功能鑒定的擬南芥在含潮黴素（ 25mg ）的ms培養基上篩選，獲得t ；代轉基因植株。The promoter - probe vector phn117 in e. coli and phn127 in g " were further constructed by removing promoter while keeping sd sequence from phn115. a teta / tetr bidirectional promoter fragment from pbr322 was respectively cloned into phn117 and phn127 and the resulted colonies were all fluorescent
並經插入pbr322上665bpteta tetr雙向啟動子片段后得到的轉化子均發綠色熒光驗證，實現了wtgfp在大腸桿菌和華癸中生根瘤菌中的組成型表達。Sequences flanking tn5 - 1063a can be recovered from the genome of mutant by excision, self - ligation and transfer to e. coli. the total dna of mutant was excised with ecori, which cut the genome frequently but not cut the transposon. after sequencing the self - ligated transpon, dna fragment flanking tn5 was obtained. the result showed 042bm - x1 contains a tn5 insertion in the gene smc00190, which function was unknown and was demonstrated to be related to salt tolerance by this study, and the gene was named as rst - 0x1
通過ecori酶切突變株基因組，得到完整的tn5 （含有在大腸桿菌中起始復制的oriv ）及其側翼的序列片段，該片段自連后轉化大腸桿菌，以tn5兩端已知的序列設計引物進行測序。 blast的分析測序結果表明， 042bm - x1和042bm - x2中tn5分別定位在苜蓿中華根瘤菌1021染色體上smc02682和smc00419基因內部，本實驗證明它們和042bm耐鹽相關，命名為rst - 0x1和rst - 0x2基因。Combining the analysis of the conservative region of 16s rrna gene of prokaryote, such as e. coli, rhizobia and several frankia strains, we designed several sets of primers to amplify the 16s rrna gene of the frankia strains tested. through tentative experiments with these primers, we screened out primers uf / ur and ec27f / frl717r
通過比較已發表的原核生物，如大腸桿菌、根瘤菌和弗蘭克氏菌的16srrna基因全序列的保守區，設計了8對引物並篩選出可適用於擴增13株供試菌株16srrna基因接近全長序列的引物： uf ur和ec27f fr1717r （產物大小約1500bp ） 。分享友人