桿狀核 的英文怎麼說
中文拼音 [gǎnzhuànghé]
桿狀核
英文
band form nucleus-
Construction of baculovirus transfer vectors for expression of core antigens of newcastle disease virus
核心抗原重組桿狀病毒轉移載體的構建They are also the key components of insect - baculovirus expression system ( bevs ), which has been one of the four main expression systems in gene engineering
同時，桿狀病毒昆蟲細胞表達系統是一類重要的真核表達系統，現已成為目前基因工程四大表達系統之一。The protein product of meq gene was highly expressed in the nuclei of recombinant baculovirus infected sf9 cells when using an anti - meq monoclonal antibody ( mcab ) 23b46 to run the immunofluorescence assay ( fa ) ; the expression quantity and if staining patterns differed with different times post - infection ( pi ). the results of western blotting and immunoprecipitation test showed there were two specific bands around 60 kd. the results of the study demonstrated that the baculovirus / insect cell system is effective to be used to express nuclear protein of virus
結果發現:本表達系統產生的meq蛋白可被重組痘病毒表達的meq制備的單抗23b46所識別;在感染細胞中， meq蛋白僅局限於細胞核內，而且隨著感染后（ pi ）時間的增加，具有從核質向核仁和核膜轉移的趨向; w已stemblot和免疫沉澱試驗均證實重組桿狀病毒感染細胞裂解物中出現有兩條大小約為60kd的特異帶。In the experiments discussed in chapter 5 we generated two recombinant viruses based on an acmnpv - and hasnpv - bac - to - bac system, respectively. in such recombinant viruses the busuctl gene under polyhedrin promoter was inserted into polyhedrin gene locus. a preliminary bioassay was conducted
第五章利用桿狀病毒bac - to - bac系統構建了含有油桐尺蠖核多角體病毒的類蝸牛毒素基因的重組病毒racbacctl和rhabacctl ，在其相應宿主甜菜夜蛾和棉鈴蟲的細胞水平和蟲體水平進行了超表達實驗。N / med tuberculosis bacili falls in dry state, also have the comfortable stress of heat - resisting
結核桿菌在乾燥狀態下，也有耐熱的適應力。A band neutrophil is seen on the left, and a large, activated lymphocyte on the right
左上是一桿狀核嗜中性粒細胞，右下可見一個大的、已活化的淋巴細胞。 ）In order to further investigate the role of axudl in human tumor carcinogenesis and the potential association between the axudl gene expression status and the stimulation of transforming growth factor beta in human cancers, the present study was performed in three aspects as follows : ( 1 ) cloning full length enconding region cdna of axudl and construction of eukaryotic vector that expression the fusion protein of axud1 and influenza virus hemagglutin ha epitope tag ; ( 2 ) exploring the time and dose effects of tgf - 1 on the expression - of axudl gene in hepg2 hepatoma cells and spc - a1 lung carcinomas cells, and studying the effects of overexpression of axud1 on the expression of cell cycle and apoptosis related protein in hepg2 hepatoma cells ; ( 3 ) construction and expression of human axudl in e. coli m15. the following main results and conclusions can be obtained from the present study : 1. the full length ecnoding region of human axudl cdna from human peripheral blood lymphocytes was successfully cloned using one step rt - pcr method, and constructed into a eukaryotic expression vector which can be expressed a ha - axud1 fusion protein with axud1 and influenza virus hemagglutin ha epitope tag. the recombinant plasmid was identified by polymerase chain reaction, restriction endonuclease maping and sequencing, this expression vector might be instrumental to further study the function of axud1 protein in tumor cells
為了進一步研究axud1在人類腫瘤發生中的作用及axud1基因的表達狀況與tgf -介導的信號通路的關系，本實驗研究分為三個部分： （ 1 ） axud1基因cdna全長編碼區的克隆和ha表位標記的axud1基因表達載體的構建； （ 2 ）探討肝癌細胞hepg2和肺腺癌spc - a1細胞中tgf - 1誘導的axud1基因表達的時間、劑量效應以及誘導表達的可能機理，並研究axud1的過表達對細胞周期和細胞凋亡相關蛋白表達的影響； （ 3 ） axud1原核表達載體的構建及其在大腸桿菌中的表達。本實驗的主要結果和結論如下： 1利用一步法rt - pcr成功地從人類外周血淋巴細胞中克隆出axud1基因編碼區cdna ，並將其構建入真核表達載體中，編碼的ha - axud1融合蛋白帶有流感病毒凝血素ha的表位標記肽段。To obtain large amounts of appa phytase, the appa gene was subcloned into the prokaryotic expression vector pet - 28a ( + ) and baculovirus transfer vector pvl - 1393 under the control of the lac and polyhedrin promoter, respectively. the appa phytase was overexpressed in e. coli strain bl21 induced by lactose
為了大量表達appa植酸酶，我們將appa基因分別克隆至原核表達載體pet - 28a （ + ）和桿狀病毒轉移載體pvl - 1393中，將其分別置於lac和polyhedrin啟動子控制之下。Based on the analysis of present situation, this paper advances the approaches to maintaining advanced nature of the party membership in colleges and universities : firstly, improving political awareness through persistent party spirit education ; secondly, improving the sense of honor and responsibility by cultivating campus culture ; thirdly, providing the system safeguard by strengthening the evaluation mechanism ; fourthly, innovating the carrier of advanced nature maintaining with the image improvement program ; fifthly, enhancing the self - discipline by implementing self - education, self - management and self - supervision
筆者從黨員教師先進性要求的內涵及現狀分析著手，提出保持高校黨員先進性的對策： （ 1 ）持之以恆地開展黨性教育，提高黨員教師的思想政治素質； （ 2 ）努力營造校園環境，提高黨員教師的政治榮譽感和責任感； （ 3 ）健全考核機制，為保持黨員個體先進性提供製度保障； （ 4 ）實施黨員形象工程，創新黨員保持先進性的載體； （ 5 ）啟動自我教育、自我管理和自我監督標桿，增強黨員教師自律意識。This product is mainly used for prestressed and post - tensioning technology in large, medium and small sizes of various of pc structures, and widely used for civil engineering construction of large scale railroad, high - way bridges, buildings, truesses, overhead crane beams, industrial and civil prefabricated concrete floor, wall board, trbular piles, power line poles, power line poles, pc water pipes, tv towers and nuclear power station, etc
主要用於製作先、后張法的大、中、小型各種結構形狀的預應力混凝土構件，如鐵路、公路、屋架、吊車梁、工業民用預制板、墻板、管樁、電桿和預應力混凝土水管、電視塔、核電站等工程建設上。The data used by calculation program and post - processing are formed by dialog boxes, and fed back by figure. a finite element program which considers the influence of axial deformation can analyze the prestressed concrete frame structure, then the structure is designed according to the code of concrete structure gbj10 - 89. post - processing can give the result of calulation by text file and graph, and plot construction drawings in autocad
前處理模塊通過對話框採集數據來建模，並利用圖形反饋建模結果；計算核心利用考慮了軸向變形的桿系有限元程序計算荷載效應和預應力效應，並按《混凝土結構設計規范》 gbj10 ? 89計算承載能力極限狀態和正常使用極限狀態；后處理模塊通過文本文件和圖形顯示來完成結果查詢，並直接在autocad環境下生成施工圖。分享友人