桿粒 的英文怎麼說

中文拼音 [gǎnlì]
桿粒 英文
bacmid
  • 桿 : 桿名詞（桿子） pole; staff
  • 粒 : Ⅰ名 （小圓珠形或小碎塊形物） small particles; grain; granule; pellet Ⅱ量詞（用於粒狀物）
  1. The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss

    將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。
  2. Obtaining transgenic male sterile tobacco in order to prove that hsp70 antisense cdna can lead to male sterility, with plasmid 3301 + 650, 3301 + 651 we transformed 207 aspetic tobacco leaves by genegun bombarding and agrobacterium mediation ( 109 by genegun bombarding, 98 by agrobacterium ). by cultivating them in blotting media containing basta 0. 4 mg / 1, we get 181 resistant leaves ( 98 by genegun bombarding, 88 by agrobacterium mediating )

    獲得轉基因雄性不育煙草為了證實hsp70反義cdna能創造雄性不育，我們將3301 + 650和3301 + 651質粒用基因槍和農桿菌介導法轉化煙草無菌發芽的葉片，共207片（基因槍109片，農桿菌98片） 。在含basta0 . 4mg l的篩選培養基上進行篩選，得到抗性葉片181片（基因槍93片，農桿菌88片） 。
  3. E. coli xl1 - blue cells were tansformed by psurfpga and phages were rescued by m13ko7 helper phage particles. results showed that the heterodimeric enzyme was expressed as a fusion protein that matures to an active biocatalyst connected to the coat protein of phage fd

    以構建的噬菌粒psurfpga轉化具有琥珀突變的大腸桿菌xl1 - blue ，以輔助噬菌體m13k07超感染，進行青霉素g酰化酶基因的表達和在噬菌體表面的展示。
  4. The mechanism enhancement of the optical brightener is not known. shapiro et al. postulated that selected brightener including m2r inhibit or alter the chitinous peritrophic membrane ( pm ), creating gaps in the membrane or gut lining and perhaps allowing more virions to pass from the gut lumen into the hemocoel

    光增白劑對桿狀病毒的增效作用的機理存在兩種推測一種觀點認為光增白劑是通過破壞圍食膜結構的完整性，促使更多的病毒粒子穿越圍食膜而發動感染的；另一種意見認為光增白劑能延遲中腸上皮細胞的脫落，促進病毒的復制繁殖。
  5. In this study, iltv - nm98a strain and iltv - wanggang strain were multiplied in chorioallantois. a pair of primers were devised according to the nucleic acid sequence of iltv tk gene and the dna of multiplied virus was used as pattern to amplify the gene of tk by polymerase chain reaction ( pcr ). the product of pcr was linked with suitable plasmid. then, the recombined plasmid was converted to escherichia coli. the converted escherichia coli

    根據已發表的iltvtk基因的核苷酸序列設計一對pcr引物，以增殖的兩株iltv的dna為模板，分別對它們的tk基因進行pcr擴增。將回收的pcr產物連接到適當的質粒載體上，轉化感受態大腸桿菌，通過篩選對iltvtk基因的陽性克隆進行擴增培養。
  6. Using enterobacter cloacae b8, the mutated strains b8b and b8f, and the recombinant clones pb and pf, we try to sequence the antagonistic - related genes of enterobacter cloacae b8 by subcloning and genome primering system. the acquired sequences were analyzed with blast program to find any homology to sequences deposited in genebank

    以廣譜拮抗菌陰溝腸桿菌b8菌株和拮抗活性缺失菌株b8b 、 b8f及從b8b和b8f二菌株克隆獲得的重組質粒pb 、 pf為基礎，對陰溝腸桿菌b8菌株拮抗相關的b和f基因片段進行序列分析。
  7. The degree of ultrastructural changes of rhabdom during light and dark adaptation of compound eye had something to do with the intensity and wavelength of environmental light in m. nipponense

    線粒體為光感受膜的合成和降解過程提供能量。日本沼蝦復眼感桿束明、暗適應時結構變化程度與外界光強度和光波長度有關。
  8. Of the eons of geological periods recorded in the stratifications of the earth : of the myriad minute entomological organic existences concealed in cavities of the earth, beneath removable stones, in hives and mounds, of microbes, germs, bacteria, bacilli, spermatozoa : of the incalculable trillions of billions of millions of imperceptible molecules contained by cohesion of molecular affinity in a single pinhead : of the universe of human serum constellated with red and white bodies, themselves universes of void space constellated with other bodies, each, in continuity, its universe of divisible component bodies of which each was again divisible in divisions of redivisible component bodies, dividends and divisors ever diminishing without actual division till, if the progress were carried far enough, nought nowhere was never reached

    隱藏在大地的洞穴里和能移動的石頭底下蜂巢和土墩子中那無數微小的昆蟲類的有機生物：微生物病菌細菌桿菌精子憑著分子的親和之凝聚力而粘在一根針尖上那幾萬幾億幾兆個多不勝數肉眼看不到的微小顆粒人類的血漿是一個宇宙，群集著白血球和紅血球，每個血球又各自形成一個空虛的宇宙空間，群集著其他球體各個球體連續性地也是由可分割的構成體形成的宇宙，各個構成體又可以分割成為幾個能夠進一步分割的構成體。就這樣，分子與分母實際上在並未分割的情況下就不斷地減少了。如果這個過程延續到一定時候，就永遠在任何地方也不會達到零。
  9. A double - strand cdna fragment of dh ( diapause hormone ) gene was obtained from kt - pcr amplification. the fragment was digested by two restriction enzyme nde 1 / ecor 1 and then was joined with pet - 30a ( + ) plasmid which was digested by nde 1 / ecor 1 too. then the outcome was transformed into escherichia coli bl21

    Pcr產物經nde / ecor雙酶切后，與同樣雙酶切的pet - 30a （ + ）質粒連接並轉化至大腸桿菌（ escherichiacoli ） bl21中，經檢測篩選，成功得到陽性克隆。
  10. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  11. The highest antiviral litre of rpoifn - a was l l08iu / mi. the rpoifn - a protected pk - 15 cells against virulent hog cholera virus ( hcv ) infection in vitro

    Sds - page分析表明，構建的重組表達質粒pqe30 poifn在大腸桿菌jm109中表達的rpoifn占菌體蛋白總量的20 26 。
  12. Sicp / al matrix composites, with 5, 15 and 25 % volume fraction of sic particles, were prepared by vacuum hot - pressing sintering processing in this paper. based on mechanics properties, sem observation and energy dispersive x - ray analysis, the interface reaction phenomenon of sicp / al composites made by vacuum hot - pressing sintering, as well as the reinforcement and fracture mechanisms of this composite were analyzed. the dynamic responses of sipc / al composites were studied by a split hopkinson high - speed pressure bar impact system which strain rate was from quasistatic state strain rate ( 3. 3 10 - 3s - 1 ) to dynamic state strain rate ( 5. 2 103 s - 1 )

    本研究以武裝直升飛機防護裝甲材料為研究對象，採用真空熱壓粉末冶金燒結工藝制備了含sic顆粒體積分數分別為5 、 15和25的sic顆粒增強鋁基復合材料，結合其力學性能、掃描電鏡和界面微區能譜分析結果，分析了sic _ p al復合材料的真空燒結過程中的界面現象，以及材料增強和斷裂機理，並利用hopkinson高速壓桿沖擊實驗系統對其從靜態到動態（應變率為3 . 3 10 ~ （ - 3 ） s ~ （ - 1 ） 5 . 2 10 ~ 3s ~ （ - 1 ） ）的壓縮破壞響應進行了研究，分析了不同體積分數sic _ p al復合材料高應變率壓縮載荷下，材料的變形和微觀損傷機理，以及利用高速沖擊空氣炮測定了改復合材料制備剃度復合板的穿透性能。
  13. Conclusions : prokaryotic and eukaryocytic expression plasmids of the shortened hepatitis b surface antigen were successfully constracted, and the target proteins expressed by iptg induced in escherichia coli. as well as in eukaryocyte ( hepg2 and cos - 7 ), then their antigenity were detected

    結論：截短的乙型肝炎表面抗原分子的原核和真核表達』重組質粒成功被構建及分別在人腸桿菌efl得到誘導表達和存貞核細胞ifj表達，並檢測劍其表達產物的抗原特性。
  14. The results indicated that : jaj could selectively stimulate the reprduction of bifidobacteria in vivo and inhibit the growth of e. coli which is a main parasitic basterium in human intestinal tract ; moreover, jaj could apprarently improve intestinal tract function. in tested group, the mice excreted smoothly and the faecal particles of mice were big and wet, but in control group, the faecal particles of mice were small and dry. lt was suggested that inulin may be the important effective component in jaj which promoted the reprduction of bifidobacteria in vivo. at last, the effects of ja on the bile salt resis tance of bifidobacteria were studied. the test proved that : deoxycholic acid na - salt ( dca - na ) had intensely toxical action on blm and bbm ; adding glucose and fructose in media could decrease the lexical action on bbm. but inulin and jap had not apparent effect

    在通過單菌株檢驗和混菌檢驗確立了一種選擇性雙歧桿菌培養基之後，進一步以健康昆明系小鼠為實驗動物，研究了菊芋在動物腸道內對雙歧桿菌的影響，動物實驗結果表明，菊芋汁在體內對雙歧桿菌有選擇性促進生長作用，而腸道中主要條件致病菌?大腸桿菌的生長受到抑制；菊芋中的菊糖成分可能對菊芋在體內選擇性地促進雙歧桿菌生長起了主要作用；此外，菊芋還具有明顯的整腸作用，同對照組相比，飼喂菊芋汁的小鼠排便順利，糞便顆粒大且濕潤。
  15. Stick-slip flow is a jerky movement of compacted grains with velocity of particles at the walls slightly lower than in the rod like core.

    粘附滑移流動是一種密集顆粒的急跳式的運動，在壁面處，顆粒的速度稍低於呈桿狀的中心的速度。
  16. There were two plasmids in the isolated lactobacillus plantarum

    分離的受體菌植物乳桿菌中含有兩種質粒。
  17. The materials as explant in transformation come from birch leaf, stem segment and leaf stalk, and the spider toxin gene was used as foreign gene for this transformation experiment. it showed that the best explant was the big leaf, on which the transformation frequency was 22 %. by gus detection, there were 43 percent of the plants with kanamycin resistance, and 100 percent of positive result, by pcr amplification, was gotten from random sampling

    利用雙元載體的根癌農桿菌lba4404菌株（ agrobacteriumtumefaciens ） ，含質粒pyhy （目的基因及npt 、 gus基因） ，對白樺試管苗莖段，葉柄，葉片三種外植體進行侵染，結果表明：大葉片生長勢強，為轉基因的最優外植體，轉化率能夠達到22 。
  18. Sub - - clone of s, . / hbsag fusion gene : pbuescripts, . / hbsag and ppiczaa were digested separately by xhoi and xbai enzyme, and were linked under t4 dna ligase, ppiczaa s, / hbsag was constructed and transformed to e. coli

    Hbsag質粒與ppiczaa載體分別經xhol和xbaln切，再在t4dna連接酶作用下進行連接，獲得工程菌表達型ppiczaas ； hbsag質粒，轉化大腸桿菌t0p10細胞，經xhol和xbal與sacll和xbal酶切電泳，證實s ； 。
  19. The recombinant plasmid puge dna and transfer vector pfastbacl dna were treated again in the same enzyme, were linked by means of t4 dna ligase and transformed into e. coli jm109 permissive cells, yielding recombinant transfer vector plasmid pfastbac - ge dna and were transformed into dhlobac containing vector bacmid

    將重組質粒pugedna與轉移載體pfastbacldna用bamhi和ecori雙酶切處理， t _ 4dna連接酶連接，用連接產物轉化大腸桿菌jm109感受態細胞，得到重組轉移載體質粒pfastbac - gedna 。
  20. Thus, it is postulated that in the presence of mild neutropenia, whose function is inhibited by effect of insulin excess, the bacillus was able to find a port of entry, probably via micro - abrasions of the bowel mucosal lining

    因此，推測可能存在中性粒細胞減少，功能被過多的胰島素抑制，芽孢桿菌可以乘虛而入，很有可能通過腸內壁粘膜微破損進入循環。
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