植物克隆載體 的英文怎麼說

中文拼音 [zhíwùkèlōngzǎitǐ]
植物克隆載體 英文
plant cloning vector
  • 植 : Ⅰ動詞1. （栽種） plant; grow; cultivate 2. （樹立） establish; set up Ⅱ名詞（姓氏） a surname
  • 物 : 名詞1 （東西） thing; matter; object 2 （指自己以外的人或與己相對的環境） other people; the outsi...
  • 克 : 克i 動詞1 （能） can; be able to 2 （克服; 克制） restrain; control 3 （攻下據點; 戰勝） overcome...
  • 隆 : 隆Ⅰ形容詞1 （盛大） grand2 （興盛） prosperous; flourishing; thriving 3 （深厚; 程度深） deep; in...
  • 載 : 載Ⅰ名詞（年） year : 一年半載 six to twelve months; six months to a year; 三年五載 three to five ...
  • 體 : 體構詞成分。
  • 植物 : plant; flora; botany; stray; greenery; phyton; phytum; phyta; phyt ; phyto ; phyte : 草本植物 her...
  • 載體 : [化學] carrier; supporter; isotopic carrier
  1. The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss

    將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。
  2. Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l. cv hetao flesh, which was cloned into plasmid vector pmd - 18 - t. the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed. at the base that pollination and fertilization of cucumis melo l. cv hetao was studied, using pollen tube pathway transformate cucumis melo l. cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed

    本實驗以河套蜜瓜果肉基因組dna為模板，用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增，得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上，篩選反向克隆，然後將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子「 」的下游，構建成反義表達載體pbinya 。並在對河套蜜瓜授粉受精生物學研究的基礎上，通過花粉管通道法轉化河套蜜瓜，共獲76顆瓜，並進行了硬度和含糖量的分析。
  3. Cloning of monomial promoter of actin1 gene and construction of plant expression vector

    1的克隆及植物表達載體的構建
  4. As a popular vegetable, tomato is rich in vatamin a, b, p and other nutrients ; among them, lyxopene can prevent prostate gland tumor. so introducing stilbene synthase gene into tomato to get new health - protection tomato will have important social and economic effect. our research is made up of four parts ; the cloning and sequencing of stilbene synthase ; tomato genetic transforming mediated by agrobacterium - lba4404 ; quantification of resveratrol in transgenic tomato by hplc analysis

    本研究包括四個方面的內容：芪合酶基因（ stilbenesynthasegene ）的克隆與全序列分析；芪合酶基因（ stilbenesynthasegene ）植物表達載體的構建；農桿菌lba4404介導的番茄遺傳轉化： hplc技術分析轉基因植株中表達白藜蘆醇的含量。
  5. Green fluorescent protein ( gfp ) gene was conjugated to the 3 " end of the pap gene in order to screen easily of the transgenic cotton plants. the combined gene was cloned into plant expression vector pbi121 and then transformed. about 5000 seeds of the transgenic cotton were obtained and the some seedlings of the transgenic cotton could give a bright green fluorescence in the dark condition when the cotton seedlings were irradiated with ultraviolet rays

    為了便於轉基因棉花後代的篩選，在pap基因的3 』端融入了綠色熒光蛋白gfp ）基因，然後將融合基因克隆在植物表達載體pbi121上，再進行遺傳轉化，得轉基因棉花種子5000餘粒，將種子播種長到于葉展開時，先在黑暗中用紫外燈照射，查找表現綠色熒光的幼苗，然後再用地高辛（ dig ）標記的pap基因特異性探針對這些棉花進行點雜交，最後發現有8株棉花表現陽性反應，說明pap基因的確己經轉到了棉花的基因組中，其棉花黃萎病的抗性鑒定正在進行之中。
  6. Since the plasmid is capable of independent replication in host cells of many dicotyledonous plants, it has been used as a cloning vector in gnetic engineering

    在許多雙子葉植物中質粒在寄主細胞中是獨立的復制單元，所以可以在基因工程中用作克隆載體。
  7. Ssmapkk transformations were screened on media with kanamycin ( 30mg / l ). nineteen individual kanamycin resistant plants were obtained. t2 plants were checked for integration of foreign gene by counting ratio of the number of tolerant plants to the number of non - tolerant plants on selection medium with kanamycin ( 30mg / l )

    將ssvp和ssmapkk的全長cdna分別克隆入植物表達載體pcambia1300和prok中，導入根瘤農桿菌gv3101后，由花浸泡法進行擬南芥遺傳轉化，轉化ssvp鹽地堿蓬ssop和ssmapkk基因的克隆與功能鑒定的擬南芥在含潮黴素（ 25mg ）的ms培養基上篩選，獲得t ；代轉基因植株。
  8. A binary plant expression vector with osg6b " driving report gene gus was constructed and transferred into tobacco via agrobacterium tumefaciens mediation

    將克隆的啟動子與報告基因gus相連，構建植物表達載體，通過農桿菌介導轉化煙草。
  9. Cloning of egf gene from mouse kidney and construction of its plant expression vector

    基因的克隆及其植物表達載體的構建
  10. Cloning of a carrot gene encoding antifreeze protein and construction of its plant expression vector

    胡蘿卜抗凍蛋白基因克隆及植物表達載體構建
  11. Plant expression vector pespcema was constructed after subcloning in puc121 and other vectors

    經puc121等中間載體的亞克隆，構建了pespcema植物表達載體。
  12. The chloroplast shsp gene was screened from the cdna library of tomato flower by pcr strategy and confirmed by sequencing. but difference was found at 3 bases of the sequence from the reported in genbank. then, an integrated vector prok ii of the chloroplast shsp gene and nptii gene ( a kanamycin resistant gene ) with camv35s promoter was constructed and introduced into tomato mediated by agrobacterium tumefaciens lba4404. transgenic tomato were screened by their ability of growing on media containing kanamycin

    本實驗採用pcr方法從番茄花cdna文庫中克隆到葉綠體shsp基因，經測序證實與genbank中已發表的序列在編碼區相差2個堿基，其中一個堿基導致1個氨基酸的改變。將葉綠體shsp基因定向克隆于帶有組成性表達啟動子camv35s的植物表達載體prok中，凍融法轉化農桿菌lba4404 ，利用葉圓盤法對番茄進行ti質粒介導的遺傳轉化。
  13. The results were as following : 1. construction and identifcation of recombinant plant expression vector pbi ! 2i - th by dna recombination technology, sweet protein thaumatin gene was cloned into plant expression vector pbii2i. recombinant plasmid pbii2i - th was constructed successfully by enzyme cutting and electrophoresis

    甜蛋白thaumatin基因植物表達質粒pbi _ （ 121 ） - th的構建與鑒定利用dna重組技術，將植物甜蛋白thaumatin基因克隆至植物表達載體pbi _ （ 121 ）中，通過酶切、電泳，鑒定thaumatin基因已成功構建到植物表達質粒pbi _ （ 121 ）中。
  14. To allow secretion of the crylac protein into the intercellular space, potato proteinase inhibitor ii ( pinll ) signal peptide sequence was n - terminally fused to the crylac coding region to construct plasmid p3301ubisigac

    運用pcr技術克隆了馬鈴薯蛋白酶抑制劑基因的信號肽序列，並將其分別連到crylac 、 gfp基因的5 』端，構建植物轉化載體p3301ubisigac和p3301ubisiggfp 。
  15. 2. an anther specific chimaeric male sterile gene expression box with a enhanced promoter ( ta29 ) driving coda gene was constructed and the expression box was inserted into binary vector p3301 that contains a l - phosphinothricin ( ppt ) - resistant selective marker gene and - glucuronidase ( gus ) reporter gene in t - dna region

    以增強的ta29啟動子驅動克隆的coda基因，構建成花藥特異性嵌合基因表達盒；將此表達盒插入雙元載體p3301 ，構建成以ppt抗性基因為選擇標記，以gus為報告基因的植物表達載體。
  16. Svde gene was also amplified from spinach by rt - pcr. the plant expression vector pcb - antisvde was constructed, in which svde gene was controlled by camv35s promoter and nos terminator. antisense transgenic tobacco plants v. ere obtained from leave explants via agrobacterium tumefaciens - medialed transformation

    從菠菜中克隆了svde基因，並構建了該基因的反義抑制植物表達載體pcb - antisvde ，用根癌農桿菌介導法轉化煙草，獲得了大量的轉基因植株。
  17. We confirmed the correct construction by pcr and restriction enzyme analysis. in this research, hypocotyls were used as the explant and several factors affecting genetic transformation of carrot mediated by agrobacterium tumefaciens were studied

    利用vp7基因和質粒pbi121上相同的單克隆位點，將vp7基因定向克隆到植物表達載體pbi121上，構建了pbi121vp7表達載體。
  18. Based on cdna of tps1 gene, pcambia1303 plasmid, we have succeeded to construct plant reombinant vector phfsm001transforming the construct plant expressing vector to immature of wheat embryo by gene gun, the wheat plant possessing the tpsl gene is attained by tissue culture and hygromylin selected

    本文以tps1基因的cdna ，質粒p ~ （ cambia1303 ）為研究對象，運用基因克隆技術，成功構建了植物表達載體phfjm001用基因槍轉化小麥幼胚，經組織培養和潮黴素篩選得到了含有海藻糖合成酶基因的轉基因小麥植株。
  19. Zhonghua 11 ) at different growth phases. so, the promoter region ofosdd2 gene was cloned and fused with a gus gene, in order to speculate the osdd2 expressing patterns

    鑒于基因表達模式不清，克隆了osdd2基因約2kb左右的啟動子序列並與gus基因融合，構建植物轉化載體。
  20. To explore the feasibility of ib edible vaccine, we have transformed si gene of ibv into potato and researched the immunogenicity of it ' s expression product. the findings are as follows : 1. a pair of primers for ibv si gene were designed and synthesized according to the published nucleotide sequence of ibv si gene and multiclone sites of expression vector pbi121

    為了探索ibv可食性疫苗的可行性，我們進行了轉基因馬鈴薯表達ibv免疫原基因及其表達產物免疫原性的研究，取得以下結果： 1根據周繼勇等報道的ibv - zj971毒株s1基因核苷酸序列和pbi121植物表達載體的多克隆位點，設計併合成引物，以含s1pbs質粒為模板擴增s1基因，將擴增片段定向克隆到pbi121質粒的35s啟動子下游。
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