植物載體 的英文怎麼說

中文拼音 [zhíwùzǎitǐ]
植物載體 英文
plant vector
  • 植 : Ⅰ動詞1. （栽種） plant; grow; cultivate 2. （樹立） establish; set up Ⅱ名詞（姓氏） a surname
  • 物 : 名詞1 （東西） thing; matter; object 2 （指自己以外的人或與己相對的環境） other people; the outsi...
  • 載 : 載Ⅰ名詞（年） year : 一年半載 six to twelve months; six months to a year; 三年五載 three to five ...
  • 體 : 體構詞成分。
  • 植物 : plant; flora; botany; stray; greenery; phyton; phytum; phyta; phyt ; phyto ; phyte : 草本植物 her...
  • 載體 : [化學] carrier; supporter; isotopic carrier
  1. The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss

    將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。
  2. ( 2 ) at translation level plant mutual sequence of starting translation aaca was added to start codon of t - pa gene by pcr ampliation and plant expression vector pbet was constructed

    （ 2 ）在翻譯水平上通過pcr擴增的方式在t - pa基因起始密碼子處添加了植物翻譯起始共有序列aaca ，構建了植物表達載體pbet 。
  3. Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l. cv hetao flesh, which was cloned into plasmid vector pmd - 18 - t. the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed. at the base that pollination and fertilization of cucumis melo l. cv hetao was studied, using pollen tube pathway transformate cucumis melo l. cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed

    本實驗以河套蜜瓜果肉基因組dna為模板，用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增，得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上，篩選反向克隆，然後將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子「 」的下游，構建成反義表達載體pbinya 。並在對河套蜜瓜授粉受精生物學研究的基礎上，通過花粉管通道法轉化河套蜜瓜，共獲76顆瓜，並進行了硬度和含糖量的分析。
  4. The dissertatio n constructs the index system, introduces the coefficients of development, coordination, fairness, and the coefficient of sd, which is composed by the former three and can reflects the sd overall strength of watershed, brings forward the quantative criteria of in order that the research of wrcc is based on the good watershed ecology and environment, the dissertation, according to the ecological appropriate theory, builds the logarithm normal distribution model about the relation between the growth of natural vegetation and the depth of groundwater ; based on this relation model, proposes a quantitative method of ecological water requirement ( ewr ) of natural vegetation in arid area, which utilizes the results of rs technique and the spot testing data of vegetative physiology demand

    針對流域特點建立了基於水資源的流域可持續發展評價指標體系，引入發展系數、協調系數、公平系數，以及由其構成的衡量水資源支撐社會可持續發展綜合水平與能力的可持續發展系數，提出了可持續發展的定量判別方法。為保證在良好生態的前提下進行水資源承載能力研究，論文根據生態適宜性理論，建立了乾旱區典型天然植物生長與主要環境因子的偏態單峰對數正態分佈模型。基於此關系模型，利用遙感技術成果以及植物生理需水的現場實驗數據，提出了乾旱區天然植被生態需水量計算方法。
  5. Many studies show that leafy is high homolog even among distantly related plant species. exception of these, little studies on tissue culture and transformation of ginkgo have been done. this paper emphasizes on the isolation, cloning and analysing two ginkgo orthologs of leafy from the male tree

    為此，本實驗從銀杏leafy同源基因的克隆入手，分析其雌雄株lfy基因結構差異，構建lfy基因的植物正義反義表達載體，建立矮牽牛遺傳轉化體系，以研究銀杏lfy同源基因的功能，同時建立了銀杏組織培養體系，為銀杏的遺傳轉化和提早開花結果奠定基礎。
  6. The dna sequence had a complete orf ( open reading frame ), which coded a protein of 479 amino acid residues. the protein sequence of enolase which contained the conserved domain, was homologue to enolase of other organisms. it showed 83 % ideaity and 89 % similarity compared to the enolase in chlamydomonas reinhardtii

    並將其中的一個gus基因用目的片段烯醇酶基因替換，構建了可以在植物中高效表達的載體pcambia2301g一enolase ，成功地將其轉入根癌農桿菌eha105中，為下一步進行轉基因植物的研究作準備。
  7. To verify the integration of st901 gene into transgenic plants genome, the stable transgenic plants were analyzed by pcr and southern blotting. the aberrant phenotypes were observed in pollens and anthers of the transgenic plants. most of pollen grains in the transgenic plant were distorted, shrunken, invagination and not stained with the solution of acetocarmine

    通過對轉基因植株花粉、花藥形態的觀察和花粉活力的測定，表明st901啟動子驅動的st901基因在轉基因植株中的表達造成花粉嚴重敗育，花粉粒皺縮，扁癟、塌陷，缺乏內容物；轉反向表達載體的馬鈴薯花粉育性僅為對照的5 . 2 ，育性下降94 . 8 。
  8. Cloning of monomial promoter of actin1 gene and construction of plant expression vector

    1的克隆及植物表達載體的構建
  9. Construction of an expressing vector for antisense rna - ribozyme chimeric dna sequence against tomato acc synthase gene and transformation of tomato

    核酶嵌合基因植物表達載體的構建及對番茄的轉化
  10. Deduced amino acid sequence of s1, s2, pvin were also highly homologous each other ( 98 %, 99 % in each case ). the stilbene synthase genes were excised from the plasmids by bamh i and sac i digestion and intergrated into a binary vector, pbi121 and pev2, from which the p - glucuronidase ( gus ) gene sequence had been removed by the same digestion to prepare a 35s promoter - stilbene synthase 2 - nopaline synthase polyadenylation site construct and a tfp2 promoter - stilbene synthase 1 - nopaline synthase polyadenylation site construct. the recombinant plasmids were called pbs2, pev2s 1. respectively

    用bamhi和saci同時酶切ps2 （ s2表示來自雷司令的芪合酶基因） 、 ps1 （ s1表示來自粉紅玫瑰的芪合酶基因）以及pbi121 、 pev2 ，使得s2 、 s1分別插入替代pbi121 、 pev2中的gus基因，構建成植物表達載體pbs2 、 pev2s1 ， pbs2中含camv35s組成型啟動子，使s2基因能在番茄植株的各個部位表達； pev2s1則含有果實特異性啟動子tfp2 ，使s1基因只在番茄果實中表達。
  11. As a popular vegetable, tomato is rich in vatamin a, b, p and other nutrients ; among them, lyxopene can prevent prostate gland tumor. so introducing stilbene synthase gene into tomato to get new health - protection tomato will have important social and economic effect. our research is made up of four parts ; the cloning and sequencing of stilbene synthase ; tomato genetic transforming mediated by agrobacterium - lba4404 ; quantification of resveratrol in transgenic tomato by hplc analysis

    本研究包括四個方面的內容：芪合酶基因（ stilbenesynthasegene ）的克隆與全序列分析；芪合酶基因（ stilbenesynthasegene ）植物表達載體的構建；農桿菌lba4404介導的番茄遺傳轉化： hplc技術分析轉基因植株中表達白藜蘆醇的含量。
  12. After researching the loads on dangerous rock synthetically, the author points out the development and collapse mechanism of dangerous rock : the geology foundations that forms dangerous rock are the constructional surfaces such as tectonic fracture 、 relief fissure, soft interlayer etc. the developing of dangerous rock is due to release of in - situ stress in rock 、 aeolation 、 water erosion and root flerry. the main loads leading to collapse of dangerous rock are gravity, water pressure and earthquake force

    在綜合研究危巖上的各種作用之後，本文提出了危巖的形成和破壞機理：硬質巖體中構造裂隙、卸荷裂隙、及各種軟弱夾層，結構面的的存在是危巖形成的地質基礎；地應力卸荷、風化、流水侵蝕、植物根劈是危巖發育的主要作用；重力、水壓力、地震力是危巖崩塌的主要荷載。
  13. Leaves of tobacco ( nicotiana tabacum ) were wounded, infected by agrobacterium tumefaciens strain lba4404 and gv3101 : pmp90 harboring expression vector pbgsb and pbglsb and co - cultured for 3 days in darkness on the culture medium ( ms + 0. 5mg / l 6 - ba + 0. 7 % agor ph 5. 7 )

    4以煙草（品種sr ）無菌苗葉片為外植體，採用農桿菌浸染的葉盤轉化法，用構建好的植物表達載體對煙草進行遺傳轉化。外植體置於無抗生素的誘芽培養基（ ms 0
  14. Expression of this truncated gene in plant was expected to give information about expression in plant of high g + c content genes but no antifungal activity was expected in this stage

    由於載體中2 . 7kb的pks基因編碼了兩種酶活性的結構域而不是完整的型pks模塊，因此我們目前並不期望這個截短的pks基因在植物能產生抗真菌活性。
  15. Deficiency of apoe may promote to produce and develop atherosclerotic lessions. the apoe gene - targeted mice will result in marked regression of both early and advanced atherosclerotic lesions by injected apoe recombinant protein, or by transfected adviral vector with apoe cdna to express human apoe transgene in liver, or by transplantation of bone marrow with normal rat apoe gene. this demonstrates that apoe gene and its expressing product can inhibit progression of atherogenesis. apoe3 has a more effective prevention from as than apoe2 and apoe4

    Apoe的缺失可促進動脈粥樣硬化的發生發展，給apoe基因敲除鼠反復注射apoe重組蛋白、在肝組織中用腺病毒載體表達apoe蛋白、移植帶有正常apoe基因的小鼠骨髓，都能使apoe基因敲除鼠的動脈粥樣硬化得到回復，表明apoe基因及其表達產物對動脈粥樣硬化的發生具有抑制作用， apoe _ 3對動脈硬化的阻抑作用要比apoe _ 2和apoe _ 4都明顯。
  16. This gene, artificially put under the control of camv 35s, was introduced into tobacco with the aid of agrobacterium, and the transgenic plants obtained were identified by gus staining, pcr and southern blot analysis

    將arge與組成型啟動子camv35s相連，構建植物表達載體，通過農桿菌介導轉化煙草。經過gus染色、 pcr及southern鑒定獲得了轉基因植株。
  17. To get in vivo evidences that apoplast calmodulin con 1d regulate plant growth and development process, a chimeric secretion form of calmodulin binding peptide, which contains a signal peptide, a calmodulin binding domain and a c - myc epitope was constructed. the chimeric gene was introduced into arabidopsis. it was expected that the overexpression of this chimeric protein could be secreted into cell wall and bound to apoplast calmodulin, which could reduce the apoplast calmoduin concentration to make an apoplast camodulin " antisense " plant. by observing the potential phenotype change of apoplast calmodulin " antisense " plant, the in vivo function of apoplast calmodulin on plant growth and developmental process could be speculated

    但這些多是採用生理學手段和藥理學方法而得出的體外（ invitro ）實驗結果，為了取得質外體cam在植物生長發育過程中發揮重要作用的invivo實驗證據，根據動物中的一些研究方法，本實驗設計並構建了帶有信號肽、 cam結合肽（ can小肽） 、 epitope （ c - myc ）融合基因的載體，並將融合基因通過真空滲入法轉入擬南芥，預期過表達的融合蛋白將會被分泌到細胞外並與質外體cam相結合，這樣就會抑制質外體cam的功能，從而可以構建質外體cam的「反義」植株，通過觀察質外體cam 「反義植株」的表型改變，就可以推斷質外體cam在植物生長發育過程中的功能。
  18. Construction and transformation of inverted repeat of turnip mosaic virus coat protein gene

    基因反向重復植物表達載體的構建及遺傳轉化
  19. Green fluorescent protein ( gfp ) gene was conjugated to the 3 " end of the pap gene in order to screen easily of the transgenic cotton plants. the combined gene was cloned into plant expression vector pbi121 and then transformed. about 5000 seeds of the transgenic cotton were obtained and the some seedlings of the transgenic cotton could give a bright green fluorescence in the dark condition when the cotton seedlings were irradiated with ultraviolet rays

    為了便於轉基因棉花後代的篩選，在pap基因的3 』端融入了綠色熒光蛋白gfp ）基因，然後將融合基因克隆在植物表達載體pbi121上，再進行遺傳轉化，得轉基因棉花種子5000餘粒，將種子播種長到于葉展開時，先在黑暗中用紫外燈照射，查找表現綠色熒光的幼苗，然後再用地高辛（ dig ）標記的pap基因特異性探針對這些棉花進行點雜交，最後發現有8株棉花表現陽性反應，說明pap基因的確己經轉到了棉花的基因組中，其棉花黃萎病的抗性鑒定正在進行之中。
  20. Plant defensin afp ( 238bp ) was amplified with six long complementary primers after two rounds of pcr amplification and plant expression vector peafp was constructed. 3

    通過合成六條長鏈引物，經兩次pcr擴增，獲得了238bp的植物防禦素afp基因全長，並構建了植物表達載體peafp 。
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