植物克隆 的英文怎麼說

中文拼音 [zhíwùkèlōng]
植物克隆 英文
plant clone
  • 植 : Ⅰ動詞1. （栽種） plant; grow; cultivate 2. （樹立） establish; set up Ⅱ名詞（姓氏） a surname
  • 物 : 名詞1 （東西） thing; matter; object 2 （指自己以外的人或與己相對的環境） other people; the outsi...
  • 克 : 克i 動詞1 （能） can; be able to 2 （克服; 克制） restrain; control 3 （攻下據點; 戰勝） overcome...
  • 隆 : 隆Ⅰ形容詞1 （盛大） grand2 （興盛） prosperous; flourishing; thriving 3 （深厚; 程度深） deep; in...
  • 植物 : plant; flora; botany; stray; greenery; phyton; phytum; phyta; phyt ; phyto ; phyte : 草本植物 her...
  1. The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss

    將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。
  2. Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l. cv hetao flesh, which was cloned into plasmid vector pmd - 18 - t. the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed. at the base that pollination and fertilization of cucumis melo l. cv hetao was studied, using pollen tube pathway transformate cucumis melo l. cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed

    本實驗以河套蜜瓜果肉基因組dna為模板，用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增，得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上，篩選反向克隆，然後將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子「 」的下游，構建成反義表達載體pbinya 。並在對河套蜜瓜授粉受精生物學研究的基礎上，通過花粉管通道法轉化河套蜜瓜，共獲76顆瓜，並進行了硬度和含糖量的分析。
  3. Due to the unevenness of burial in horizontal space, genet or clonal fragment of clonal plants often incurs partial sand burial

    由於沙埋在水平空間表現出非均勻性，克隆植物的基株或克隆片斷常常經歷局部沙埋。
  4. This environmentally induced division of labor benefits the whole genet greatly, and th us is another adaptive strategies of clonal plants to grow successfully in heterogeneous environments

    這種環境誘導的克隆內分工行為有利於整個基株對資源交互斑塊性環境的利用，是克隆植物對異質性環境的生態適應對策。
  5. We isolated the badh cdna ( acbadh ) from the halophyte atriplex centralasiatica iljin using rt - pcr and the deduced protein sequences showed high similarity with other plants

    我們以鹽生植物中亞濱藜（ atriplexcentralasiaticailjin ）為材料，用pt - pcr方法克隆了badhcdna 。
  6. Many studies show that leafy is high homolog even among distantly related plant species. exception of these, little studies on tissue culture and transformation of ginkgo have been done. this paper emphasizes on the isolation, cloning and analysing two ginkgo orthologs of leafy from the male tree

    為此，本實驗從銀杏leafy同源基因的克隆入手，分析其雌雄株lfy基因結構差異，構建lfy基因的植物正義反義表達載體，建立矮牽牛遺傳轉化體系，以研究銀杏lfy同源基因的功能，同時建立了銀杏組織培養體系，為銀杏的遺傳轉化和提早開花結果奠定基礎。
  7. Cloning of monomial promoter of actin1 gene and construction of plant expression vector

    1的克隆及植物表達載體的構建
  8. Gene engineering based on reducing na + toxicity creats a new approach to plant salt tolerance. this is the first time expressing of sod2 in arabidopsis thaliana and maybe attribute to research the ion homeostasis in the cell. the sod2 gene was isolated from s. pombe by rt - pcr method and confirmed by sequencing

    雖然sod2基因已經克隆，但是sod2在高等植物中異源表達及對其影響還未見報道，因此sod2基因在擬南芥中過量表達，對研究細胞的離子均衡及運用基因工程手段有效地提高植物的抗逆性具有重要意義。
  9. As a popular vegetable, tomato is rich in vatamin a, b, p and other nutrients ; among them, lyxopene can prevent prostate gland tumor. so introducing stilbene synthase gene into tomato to get new health - protection tomato will have important social and economic effect. our research is made up of four parts ; the cloning and sequencing of stilbene synthase ; tomato genetic transforming mediated by agrobacterium - lba4404 ; quantification of resveratrol in transgenic tomato by hplc analysis

    本研究包括四個方面的內容：芪合酶基因（ stilbenesynthasegene ）的克隆與全序列分析；芪合酶基因（ stilbenesynthasegene ）植物表達載體的構建；農桿菌lba4404介導的番茄遺傳轉化： hplc技術分析轉基因植株中表達白藜蘆醇的含量。
  10. It will provide us to further study the function of xanthophyll cycle in photoprotection. the major results are as following : two cdna sequences encoding violaxanthin de - epoxidase were cloned from japonica rice ( jrvde ) and indica rice ( irvde ) with the full - length of 1887bp and 1647bp, respectively. the homology of the open reading frame is 98 % identity between two rvde genes, and more than 60 % identities with those of other species

    本論文從水稻和菠菜中克隆了編碼vde酶的基因，並通過轉基因植物進一步研究了葉黃素循環在熱耗散方面的作用，主要獲得了以下結果：首次從兩個水稻亞種（秈稻和粳稻）中克隆了rvde基因（分別命名為irvde和jrvde ）的全長cdna序列，分別長1647bp和1887bp ，兩者開放閱讀框的同源性為98 ，與其它已知vde基因的同源性在60以上。
  11. Barley yellow dwarf virus often leads to a serious loss in the yield of wheat. but at present, we have not find excellent genes resistant to bydv in wheat, so transferring the excellent genes from relatives to cultivars of wheat will be a good method

    因此，植物耐鹽、耐黃矮病、耐蚜蟲等機理和相應耐性植物的培育，乃至相應耐性相天基因的分離與克隆及其基因工程育種的研究正在受到人們越來越多的關注。
  12. On the contrary, stem - derived forms of clonal plants more f requently occur in colder and drier habitats corresponding to higher latitude and / or higher elevation. individual types of clonal growth with sprouting are more frequent in warmer and wetter habitats, such as shrub forest, broad - leaves forest and bamboo forest. individual types of clonal growth with rhizome are more frequent in colder and drier habitats, such as alpine shrub forest, grassland and desert steppe

    根起源克隆植物中，根出條型植物的生境條件和根起源克隆植物的相同，出現在灌叢、闊葉林和竹林中的頻率較高；莖起源克隆植物中，根莖型植物的生境條件和莖起源植物的相同，出現在水生植被、草甸和草原中的頻率較高，而匍匐莖型植物在較溫暖、濕潤、陰郁的生境中出現頻率較高。
  13. The leymus multicaulis has many excellent traits, such as immune or highly resistant to bydv, and highly tolerant to salt and aphid, which are controlled by some unknown genes

    這些特性都是目前種植的大多數農作物、牧草與草坪草栽培品種，本身缺乏急需改良的特性。因此，分離克隆控制這些優異抗逆特性的功能基因，非常必要。
  14. 2. in order to express chs in plant, we constructed plant expression vector p1301b q c. first added camv 35s promoter and nos terminator to chs by one median clone

    （ 2 ）為了能在在植物中表達（ chs ，通過一步的中間克隆，將chs連上camv35s啟動子和nos終止子。
  15. Two complete squence primers were designed based on the result of race. a 1475bp sequence was amplified by pcr. the analysis of the product shows that the nucleotide and deduced amino acid sequences share 40 % - 60 % homologous to the corresponding parts of - glc gene family of pinus contorta, cucurbita pepo, arabidopsis thaliana by the blast _ w program comparison

    經blast搜索表明：克隆所得堿基序列和推導的氨基酸序列與已克隆出的小松樹、西葫蘆擬南芥等植物體內-葡萄糖苷酶基因的cdna相應序列有40 ? 60的同源性，因此我們推斷擴增所得到的序列為茶樹中-葡萄糖苷酶基因的cdna 。
  16. Green fluorescent protein ( gfp ) gene was conjugated to the 3 " end of the pap gene in order to screen easily of the transgenic cotton plants. the combined gene was cloned into plant expression vector pbi121 and then transformed. about 5000 seeds of the transgenic cotton were obtained and the some seedlings of the transgenic cotton could give a bright green fluorescence in the dark condition when the cotton seedlings were irradiated with ultraviolet rays

    為了便於轉基因棉花後代的篩選，在pap基因的3 』端融入了綠色熒光蛋白gfp ）基因，然後將融合基因克隆在植物表達載體pbi121上，再進行遺傳轉化，得轉基因棉花種子5000餘粒，將種子播種長到于葉展開時，先在黑暗中用紫外燈照射，查找表現綠色熒光的幼苗，然後再用地高辛（ dig ）標記的pap基因特異性探針對這些棉花進行點雜交，最後發現有8株棉花表現陽性反應，說明pap基因的確己經轉到了棉花的基因組中，其棉花黃萎病的抗性鑒定正在進行之中。
  17. Glossary of botanical terms

    中國植物克隆網
  18. Plant cloning technology combines cell culture and soilless cultivation with microcuttage so as to rapidly and numerously cultivate a variety of fine and rare sprouts in a short period and attain considerable economic benefit

    植物克隆技術將組織培養、無土栽培與微型扦插三項技術有機結合、短期內可快速、大量地培育出各種優良、珍稀種苗，經濟效益可觀。
  19. The constuction of plant expression vector, tissue culture of cabbage and gene transformation conditions were studied to obtain ubic transformants. ubic gene was obtained from the escherichia coli. by pcr amplication and was ligated to puc118 vector

    為獲得轉ubic基因甘藍，對植物基因工程載體的構建、甘藍的組織培養以及基因轉化條件進行了研究。通過pcr方法從大腸桿菌基因組中擴增得到了ubic基因，擴增產物克隆到puc118載體，轉化大腸桿菌jm109 。
  20. Sheep, mice, cattle, goats and pigs have been cloned, dolly - style, by transferring the nucleus of a cell from an adult animal into a hollowed - out egg. the success rate with cats was as low as with these animals. cc, born two months ago, was the only live birth from 87 cloned embryos that were implanted in surrogate mothers

    至今為止，科學家們已經陸續成功克隆了綿羊老鼠牛山羊和豬，這些動物克隆的方式都和第一隻克隆動物多利綿羊是相同的，即把成年動物細胞中含有遺傳物質的細胞核植入去核的卵細胞中，並使兩者融合，融合體分裂發育成克隆動物。
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