模板基因 的英文怎麼說

中文拼音 [móbǎnjīyīn]
模板基因 英文
templet gene
  • 模 : 模名詞1. （模子） mould; pattern; matrix 2. （姓氏） a surname
  • 板 : Ⅰ名詞1 （片狀硬物體） board; plank; plate 2 （專指店鋪的門板） shutter 3 [音樂] （打拍子的樂器） ...
  • 因 : Ⅰ動詞[書面語] （沿襲） follow; carry on Ⅱ介詞1 [書面語] （憑借; 根據） on the basis of; in accord...
  • 模板 : [土] formwork; mould; shuttering; follow board; form board; match board; match plate; mother plat...
  1. Now many laboratories have used avirulent strains of s. typhimurium to deliver h. pylori antigens as a way of stimulating an immune response to those determinants

    以鼠傷寒沙門氏菌標準株基因組dna作為模板，採用pcr的方法克隆了鼠傷寒沙門氏菌asd基因，經序列分析與文獻報道一致。
  2. In this study, iltv - nm98a strain and iltv - wanggang strain were multiplied in chorioallantois. a pair of primers were devised according to the nucleic acid sequence of iltv tk gene and the dna of multiplied virus was used as pattern to amplify the gene of tk by polymerase chain reaction ( pcr ). the product of pcr was linked with suitable plasmid. then, the recombined plasmid was converted to escherichia coli. the converted escherichia coli

    根據已發表的iltvtk基因的核苷酸序列設計一對pcr引物，以增殖的兩株iltv的dna為模板，分別對它們的tk基因進行pcr擴增。將回收的pcr產物連接到適當的質粒載體上，轉化感受態大腸桿菌，通過篩選對iltvtk基因的陽性克隆進行擴增培養。
  3. Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l. cv hetao flesh, which was cloned into plasmid vector pmd - 18 - t. the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed. at the base that pollination and fertilization of cucumis melo l. cv hetao was studied, using pollen tube pathway transformate cucumis melo l. cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed

    本實驗以河套蜜瓜果肉基因組dna為模板，用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增，得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上，篩選反向克隆，然後將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子「 」的下游，構建成反義表達載體pbinya 。並在對河套蜜瓜授粉受精生物學研究的基礎上，通過花粉管通道法轉化河套蜜瓜，共獲76顆瓜，並進行了硬度和含糖量的分析。
  4. By applying the th model theory to analyzing the cases of developed countries that mentioned in chapter 4, chapter 6 finds out the block gene and tendon compages that consists of the university - government - industry th. furthermore, it gives the pheno - types as some external expressions of the genotypes and the family - tree diagram constituted by these phenotypes. after a transition, it illuminates the scientometric, the webometric and the triple helix algorithm and their application

    第六章運用th模型理論對第四章提供的案例挖掘梳理后，發現並給出了：構成大學?政府?產業三重螺旋體的完整板塊配基和鍵鏈組合，作為「基因型」外在表達的一些「現象型」屬類以及由它們所構成的圖式譜系。
  5. This time, using cdna of zmcdc5 as template, we amplify a sequence by means of pcr technology. and then, using restrict endoenzyme and ligase, we conjunct the 0. 8kb length dna sequence in a expression vector, pet - 30a. after induction, expression and purification, we obtained a 35. 4kda truncated fusing zmcdc5 protein which contains 267aa ( 647 to 914aa in zmcdc5 ). with the purified protein, we got its antibody and testified the antibody by means of western blotting and dot blotting

    本實驗是以zmcdc5的cdna為模板，使用pcr獲得基因片段，再通過酶切連接，將得到的0 . 8kb的基因片段構建於pet - 30a表達載體上，經過誘導表達和純化，獲得zmcdc5的融合蛋白，其中包括了zmcdc5925個氨基酸中647 914共267個氨基酸殘基
  6. A pair of primers were chemically synthesized based on the cdna sequence of hog cholera virus strain brescia and used to amplify the cdna fragments of envelope glycoprotein e2 gene of hclv which coding the major protective antigen by reverse transcription - polymerase chain reaction ( rt - pcr ) from total intact rna extracted from infected calf testicle cell culture by hclv

    以豬瘟兔化弱毒犢牛睪丸細胞毒（ hclv ）中提取的細胞總rna為模板，以rt - pcr技術，擴增出了完整e2基因的cdna片段。經電泳、酶切分析，證實了所擴增片段為e2基因特異性片段。
  7. Guan xiaohong ( 1991 ) established a cell line of the monoclonal anti - idiotypic antibody ( anti - id ) np30 of schistosoma - 6 - japonicum, whose isotype was identified to be igm and which was testified to be internal image of gaa

    分別以日本血吸蟲單克隆抗獨特型抗體wbo的雜交瘤細胞株總基因組dna和其提取rna進行rticr合成的cdna第一鏈為模板，擴增v 。 、 v 。
  8. Cloning and expression in e. coli of lactase. specific primers were designed according to the sequence of the beta - galactosidase gene from kluyveromyces lactis. klac gene was amplified by pcr and subsequently cloned

    乳糖酶基因的克隆及原核表達以乳酸克魯維斯酵母（ kluyveromyceslactis ）菌株k538的基因組dna為模板，設計引物，利用pcr獲得乳糖酶基因klac ，經dna測序驗證，得到克隆t1549 。
  9. At the same time, using the genomic dna of blast sensitive cultivar c039 as a template, two fragments were obtained that were the same large dna sequence as resistance cultivar c101a51. one sequence has conserved motifs of nbs - lrr type resistance genes, the other sequence can " t read through

    同時本研究還以感病品種c039的基因組為模板，也擴增得到與c101a51抗病品種中抗病基因同源序列同樣大小的dna片段，但一個有抗病基因的保守結構，另一個片段不能通讀。
  10. Used the genomic dna extracted by low melting - point agarose embedding method as pcr template, the full length of structural genes of bacillus subtilis bio operon were gained by long pcr method

    將該方法提取的基因組dna稀釋100倍作為模板，採用長距離pcr方法，獲得了枯草桿菌生物素操縱子基因全長。
  11. Ghrelin regulates pituitary growth hormone ( gh ) secretion. in the study, we obtained the cdna sequences of preproghrelin and the cdna encoding the ghrelin of duck, goose and emu by using rt - pcr and 3 " - race methods. and then deduced the amino acid sequences of preproghrelin and ghrelin in duck. goose and emu. sense and antisense primers were designed on the basis of chicken ghrelin cdna sequence ( dest accession no. ab075215, 836bp ) and proventriculus cdna was performed as the template

    Ab075215 ， 836bp ）設計引物，以鴨、鵝、鴯鶓腺胃cdna為模板，通過rt - pcr及3 - race的方法，將各個產物經過回收，再經連接轉化，克隆測序，拼接后得到鴨、鵝、鴯鶓preproghrelin基因的cdna序列及ghrelin的cdna序列，並由此推測出preproghrelin及ghrelin的氨基酸序列。
  12. The supernate of virus culture was used as templet in overlapping pcr, then the interesting gene was obtained

    利用重組pcr的方法，以病毒培養液上清為模板，把二個目的基因串聯在一起。
  13. Using molecular imprinting method, the 1, 3 - dimethylxanthine theophylline, tho molecular recognition membranes, containing an segments as membrane formation sites and aa segments as functional sites, were prepared by the phase inversion technique. here, tho was selected as a template molecule. the hydrogen bonding between aa segments and the tho templates was measured by ft - ir and nmr. the tho templates can be removed from the membrane through washing with acetic acid aqueous solution. the permeation of tho through the membranes is far more than that of 1, 3, 7 - trimethylxanthine caffeine, caf, which demonstrated the function of tho molecular recognition of the membrane. the results also show that the increase of the tho templates concentration in the cast solution caused an increase of tho amounts taken into the copolymer membrane

    Ft - ir及nmr測試結果表明：制備的高分子膜中， tho模板分子和膜中的丙烯酸功能殘基存在著氫鍵鍵合作用。大量的極性醋酸水溶液可抽出膜中的模板分子。 tho溶液和與模板分子具有相似結構的1 ， 3 ， 7 -三甲基黃嘌呤咖啡因， caf溶液的基質透過實驗結果：進入膜結構中tho分子的量遠大於caf分子，這表明制備的高分子膜具有tho分子識別功能。
  14. By the technology of gene cloning, bioconversion of d - amino acids with engineered cells containing d - hydantoinase and d - carbamoylase would be expected to overcome the drawbacks presented by using the original strains described above. according to the reported amino acid sequence of d - hydantoinases, two primers were designed and synthesized

    本文根據文獻報道的海因酶基因序列及大腸桿菌對密碼子的偏愛性分別設計了正向及反向引物，以基因組dna為模板，利用pcr技術擴增得到菌株pseudomonasputidayz - 26和sinorhizobiummorelensess - ori的d -海因酶基因。
  15. Based upon the comparison of cyto b gene sequences in 15 deer species downloaded from genbank, a universal primer set l15774 / hsf21 was used as positive control of the template quality, at the meantime, two species specific primer sets df / dr and cf / cr were desgined to identify red deer ( cervus elaphus ), sika deer ( cervus nippori ) and roe deer ( capreolus capreolus ) from other species. a musk deer ( moschus ) specific primer set wf / mr was designed, siberian musk deer ( afoschus moschiferns ) and forest musk desr ( moschus berezovskii ) could be discriminated when restriction endonuclease rsa i was used to cut the pcr products

    經過對來自genbank中的15種鹿類動物的cytob基因序列的比較，用通用引物l15774和hsf21作為模板的質量控制，設計了特異性引物df dr和cf cr來鑒定馬鹿、梅花鹿、狍；設計了麝類特異性引物mf mr ，用限制性內切酶rsa酶切擴增產物來區分原麝和林麝。
  16. Structural matching is a main approach for on - line chinese character recognition. in order to reduce its great computational comple xity and improve its performance, people have been seeking for a way to guide the whole matching by the result of partial matching. in this paper, the authors prop osed 45 basic components from 3, 755 categories of the daily - used chinese charac ters to guide the stroke segment matching. because they always locate at either the beginning or the end of the stroke segment string, these components are easy to extract and separate from other parts of the character. besides, the reference templates of these components are dynamically extracted from the reference segmen tstring and dependent on the current matched character so that a more accurate matching is carried out. experiments show that the segment matching computation h as reduced almost 50 %. the approach is also enlightening for other similar object matching problem

    結構匹配是一種有效的聯機手寫漢寫識別方法，為了減少匹配運算，人們一直在尋求利用部分匹配的結果來引導整體匹配的方法.在特徵匹配與結構匹配綜合的基礎上，從3 . 755個一級國標漢字中提取出45個子結構，利用它們來引導結構匹配.由於這些子結構總出現在字首或字尾，因而對它們的檢測比較容易.同時，通過建立子結構活動模板及設計子結構動態抽取演算法，使得子結構匹配的準確度得到很大提高.實驗結構表明，該方法使結構匹配的運算量減少約50 % ，並對類似的物體識別問題有一定的啟發意義
  17. 2. obtaining of mcema ( modified cema ) and plant defensin afp gene mcema ( 141bp ) was amplified with pucspcema plasmid as template and p5, p4 as primers, and plant expression vector pemcema was constructed

    Mcema和植物防禦素基因的pcr合成以經測序驗證的spcema為模板，用p _ 5和p _ 4擴增得到了全長141bp的mcema ，並構建了植物表達載體pemcema 。
  18. It has been reported that the eiav s2 is not essential and does not appear to affect virus infection and replication in vitro. thus, we introduced a his - tag into the s2 gene of an eiav infectious molecular clone recombinant plasmid ( pok8266 ) by using soe pcr method and obtained a new recombinant plasmid with his - tag, designated as eiav - pok8. 2 - his

    本研究應用已構建好的eiav驢白細胞弱毒疫苗株的感染性分子克隆載體（ pok8266 ）為模板，通過soepcr方法在感染性分子克隆載體的s2基因獨特區內引入突變點，形成含有酶切位點（ nspv ）的突變體（ p1p4 ） 。
  19. Linearized full - length cdna was used as template then genomic rna of csfv was in vitro transcriped by t7 rna polymerase

    以線性化的全長cdna為模板，體外轉錄得到了csfv基因組rna 。
  20. Two oligonucleotides were synthesized according to the sequence of p. furiosus extracellular a - amylase gene amya through the genbank and used as primers for pcr with p. juriosus genomic dna as the template

    根據genbank公布的p furiosus的-澱粉酶基因amya序列設計兩條引物，以p furiosus的基因組dna為模板進行pcr 。
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