正染細胞 的英文怎麼說
中文拼音 [zhēngrǎnxìbāo]
正染細胞
英文
orthochromophil- 正 : 正名詞(正月) the first month of the lunar year; the first moon
- 染 : Ⅰ動詞1 (用染料著色)dye 2 (感染) catch [contract] (a disease) 3 (沾染) acquire (a bad hab...
- 細 : 形容詞1 (條狀物橫剖面小) thin; slender 2 (顆粒小) in small particles; fine 3 (音量小) thin ...
- 胞 : Ⅰ名詞1 (胞衣) afterbirth2 (同一個國家或民族的人) fellow countryman; compatriot Ⅱ形容詞(同胞...
- 細胞 : cell; sytes; bioplast; cella; [口語] gene; [生物學] cellule; cellule cellulli cellulo ; cello ; k...
-
It is an important that bacteria contaminated vaccine in the biologicals production. we collected 703 samples of cell culture, virus cultivation and harvest which were contaminated by bacteria during poliovaccine production within two years. we checked these samples by bacteriological method and antibiotics sensitivity tests were done. it shows that 1 ) the main contaminated bacteria come from staphylococci, bacilli and streptococci of environment in the poliovaccine production. 2 ) it is effect that antibiotics to contaminated bacteria are doxycycline, albiotic, prescription 2, cefotaxime na salt, gentamycin, neomycin, aureomycin and erythromycin
在疫苗生產實踐中,細菌污染是影響疫苗質量和產量的關鍵性因素,筆者通過了兩年左右的時間,選取正常生產中零星細菌污染的細胞培養瓶、病毒培養瓶及收毒污染樣品等共703份,進行細菌學檢查,並對造成污染的主要細菌種類進行了各種抗菌藥物的耐藥性實驗,結果表明:我所脊灰疫苗生產中主要的污染威脅來自環境中的葡萄球菌,潛在威脅是桿菌和鏈球菌;強力黴素、林可黴素、配方2 、噻孢黴素鈉鹽、慶大黴素、新黴素、金黴素和紅黴素等抗生素對目前引起污染優勢細菌-葡萄球菌有明顯的抑菌效果,可作為疫苗生產后備抗菌手段參考Trial 2, effect of supplemental copper of different type on nutrition and specific immunity of mice - ii the grouping of trialt animal was the same as trial 1, at the first day, second day, third day, one mouse was injected with pha brine fluid for 10mg / kg avoirdupois after weighing in the same time in each repeat, following the 7d, 14d, 21d, 8d feeding period, after weighing, blood was made, wrigh - giemsa coloration, counting the number of lymphocyte female cell and overage lymphocyte, index of immune organ, copper concentration in liver and spleen
試驗二,不同形式銅對小鼠營養與特異性免疫功能的作用-試驗動物分組同試驗一,進入正式試驗期后,在每周第1天、 2天、 3天同一時間每重復選取1隻小鼠,稱重后每天按10mg kg體重肌肉注射一次植物血凝素生理鹽水溶液,並於試驗第7天、 14天、 21天、 28天稱重后尾尖取血,姬姆薩-瑞氏染色,計算t淋巴細胞轉化率,計算免疫器官指數,測定肝臟、脾臟銅含量。Cytogenetic studies were made on several hybrid scallops using the karyotype method. hybridizations between chlamys farreri and patinopecten yessoensis, c. farreri and chlamys nobilis were studied
本論文在染色體水平對蝦夷扇貝櫛孔扇貝雜交以及櫛孔扇貝華貴櫛孔扇貝正反交等幾個扇貝雜交組合進行了細胞遺傳學研究。As for the sub - cellular locations of cortactin, laser confocal scanning microscope pictures shew that in cleavage stage, cortactin was distributed ubiquitously in the cytoplasm with the exception that in cells undertaking cytokinesis, positive stained signals were detected in the cleavage furrow
在利用激光掃描共聚焦顯微鏡進行的皮層蛋白亞細胞定位的觀察中發現,卵裂期,皮層蛋白在整個細胞質中分佈,在正在進行胞質分裂的卵裂溝中染色較強。Asymmetrical distribution of cortactin appeared when gastrulation starts, with higher staining in the dorsal part of the embryo, while much lower in the ventral part
在胚盾附近區域,即未來的胚胎背側,染色信號較強,皮層蛋白標志了正在發生匯聚遷移的細胞。Part 1 : the culture and identification of es - d3 cells and the study of the efficiency of eb formation from es cells when grown on mef feeder layer in es culture medium or cultured in es culture medium supplemented with lif 1000u / ml, es - d3 cells being used in our experiments formed normal clones, expressed akp and kept their normal karyotype over many passages. the in vitro and in vivo differentiation experiments showed that es - d3 cells could differentiate into variety of cell types derived from three primary germ layers
結果顯示: eso3細胞在小鼠胚胎成纖維細胞上和或含白血病抑制因於億f )的es細胞培養液中形成典型的胚胎幹細胞克隆,堿性磷酸酶染色結果為強陽性,具有正常二倍體核型以及具有在體內外分化為三個胚層來源的組織細胞的潛能,而且具有形成種系嵌合動物的能力。At high magnification, the neoplastic glands of adenocarcinoma have crowded nuclei with hyperchromatism and pleomorphism. no normal goblet cells are seen
高倍鏡下腺癌腺體見大量密集深染異型性明顯的細胞核。不見正常的杯狀細胞。The karyotye analysis was made on the 5th passage, the number of the chromosomes ranged from 187 to 200, and no heteroploid cell was found
對第5代的傳代細胞進行了遺傳學分析,傳代4次之後,其染色體形態正常, 4n為187一200之間,未發現明顯的異倍化現象。( 2 ) the mortality of cp cell in 10 were increasing directly related with the time in the low temperature. after 120d some cp cells died in the way of apoptosis. most nucleus of cells were condensed, and chromosome was marginated beside the nucleus inner membrane, cell sizes were reduced, dna ladder showed in dna gel electrophoresis
( 2 ) cp細胞系在10低溫下細胞死亡率與時間成正比, 120d的細胞具典型的凋亡現象,即細胞核固縮、染色體邊集在核膜內側;細胞體積變小;瓊脂糖凝膠電泳上顯示特徵性的「梯狀」帶。The bell - shaped time course of the information entropy indicates that a forward mutation of " - resistant " hosts takes place, since no loss of cellular viability occurs for the second growth phase of reinduced ( i. e. recovered ) cells
從誘導過程之鐘形訊息亂度之時間趨勢表示正向反應變異為-阻抗能力之宿主菌確實已發生,因為再誘導菌相細胞之第二生長期並未發生因感染而失去細胞存活之現象。The deleted mutant pap gene was also cloned into yeast secreted expression ppic9k vector to form ppic9k ~ 3, then the vector was transferred into pachia pastoris gs115 strain. the specific expression protein was secreted into the medium after inducing with methanol and the protein amount reached about 50 - 60 u g per millilitre measured by uv - absorbed methods in the supernatant of the medium via high density fermentation. sds - page results showed that there was one protein band in the gel which molecular weight was about 34ku
將缺失型pap基因克隆于酵母分泌型表達載體ppicgk構成重組載體,然後導入畢赤酵母( p8chianastoris )菌株gslls細胞中,在甲醇的誘導下,經過酵母高密度發酵進行pap的表達,經sds page分析,結果表明,在培養基上清液中含有一明顯的特異性蛋臼條帶,大小為34ku ,經western blotting分析,該蛋白與法國pap抗血清有特異性反應,體外活性檢測表明該蛋白對tmv的侵染性具有高度的抑制性,說明該pap基因在畢赤酵母gs中也得到了正確表達。The aglycone, steviol, exhibited greater acute toxicity than stevioside in hamsters but not in rats. steviol was clearly genotoxic after metabolic activation, inducing forward mutations in bacteria and gene mutations and chromosomal aberrations in lung fibroblasts of chinese hamsters
甜菊醇經過代謝活化后,基因毒性明顯,可引致細菌產生正向突變,以及使中國倉鼠的肺纖維原細胞產生基因突變和染色體變異。During the different time point, we retrieve the cell supernatant and through the electrophoretic analysis and immunoblot of the recombinant protein indicated that the cell which transfected the recombinant eukaryotic gene expressing vector could synthesis and excrete the peptide effectively
提取培養細胞總rna反轉錄cdna測序結果表明人生長激素基因組dna在家蠶bmn細胞內能正確轉錄,剪接。蛋白質電泳分析和免疫學檢測證明轉染細胞能夠有效合成並分泌hgh蛋白質。With a pas stain, the budding cells and pseudohyphae ( short filaments that are not true hyphae ) of candida stain bright red
Pas染色,假絲酵母的出芽細胞和假菌絲(短細絲不是真正的菌絲)被染為鮮紅色。After the recombinant plasmid pcdna3. 1 / ts87 was identified by digestion of hindlll and bamh i, it transformed into cos7 by lipofectamine. expression product was identified by immunohistochemical method, sds - page and western - blot. the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3. 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas. l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t. s artificially and sera from rabbit immunized with soluble antigen of t. s and with protein expressed by ts87 gene and by a monoclonal antibody of t. s
通過細胞的免疫組化,細胞裂解物的sds - page電泳, westem - blot分析檢測目的基因的表達情況。免疫組化結果顯示:重組質粒轉染的細胞質中有棕褐色顆粒,而空載體轉染細胞及正常細胞無此現象;細胞裂解物sds - page電泳結果顯示:只有重組質粒轉染的細胞在約38kd處有明顯的蛋白帶,這與理論計算的ts87基因表達蛋白的分子量為38kd基本一致; western - blot分析結果顯示:約38kd的蛋白帶能夠分別被旋毛蟲感染兔血清,成蟲蟲體可溶性抗原免疫兔血清, ts87基因原核表達蛋白免疫兔血清( ts87血清)以及一株具保護性的旋毛蟲單抗特異識別。P - selectin was negative both in the normal skin and in the postmortem damage group. in the wound specimens aged 1h, we detected expression of icam - 1 in the epidermis, a strong positive staining was observed in 3 hours after wound, and continued to 1 days from the skin was injured after incised, weak staining were showed on epiderm of the injury margin after 3 days incised, and disappeared in 5 days. expression of icam - 1 was also detectable in polymorphonuclear cells ( pmns ) in the wound specim - ents aged 3h
本實驗研究證明,皮膚損傷后表皮層icam1表達增高,其表達呈現一定時間規律性,在損傷后lh ,損傷邊緣表皮細胞即呈黃褐色染色,傷后3h組, icam一二在表皮層中呈深黃褐色,並一直持續至傷后id ,傷后3d組icam 1染色轉淡,呈黃褐色染色,在傷后sd及7d組, 1 m一二表達基本恢復至正常狀態。These results indicate that the alteration of cell proliferation and dna synthesis caused by different gnt - v cdna transfection may at least partly result from the modification of n - glycan structure and function of egfr. it seems that the increased 1, 6 glcnac branch on the n - glycans of egfr may benefit to its binding with egf and the resulting tyrosine auto - phosphorylatio n, while the decrease of this branch may prevent these processes
用特異性抗體結合westemblot結果發現,正義或反義gnt一vcdna的轉染並不引起pkb 、 p44 / 42mapk和mek蛋白質表達的變化,而gntv一s / h 」 21細胞pkbt308 、 5473位點磷酸化和免疫沉澱pkb的酪氨酸磷酸化以及以gsk召a /日磷酸化為指標的pkb的活性都較mock細胞增加, gntv一as / h7721細胞中這些指標的變化則相反。Are the changes in signal transduction and gene expression related to the structural alteration of the sugar chain on some surface receptors resulting from the transfection with sense or antisense gnt - v
而細胞信號轉導的改變是否與細胞表面某些受體的糖鏈結構的改變(由gnt - v的正義或反義cdna轉染引起的)有關But to virus, because it does not have ego breed ability, its are used to biosynthesis machine undertakes duplicating and release filial generation virus in human body cell with respect to parasitism, and so far, we still are mixed without can divisional normal cell by the medicaments of infection cell, impossible that all cells kill a human body
但是對于病毒,由於它沒有自我繁殖能力,就寄生在人體細胞中利用其生物合成機器進行復制並釋放子代病毒,而到目前為止,我們還沒有能區分正常細胞和被感染細胞的藥物,又不可能把人體所有細胞都殺死。The recombinant virus adhuctla4 - ig prepared in this study efliciently inltcted l - o2 " cells, and the infected cells expressed a - nd excreted soluble rccolllbina11t protein huctla4 - ig
該重組病毒在體外能有效感染正常肝細胞株l - o2 ,受感染細胞能表達、分泌可溶性的重組融合蛋白huctla4 - ig ; 2分享友人