比亞切克 的英文怎麼說

中文拼音 [bǐyǎqiēkè]
比亞切克 英文
bjacek
  • 比 : Ⅰ動詞1 （比較; 較量高下、 長短、距離、好壞等） compare; compete; contrast; match; emulate 2 （比...
  • 切 : 切Ⅰ動詞1 （合; 符合） correspond to; be close to 2 （用在反切后頭 表示前兩個字是注音用的反切）見 ...
  • 克 : 克i 動詞1 （能） can; be able to 2 （克服; 克制） restrain; control 3 （攻下據點; 戰勝） overcome...
  1. If the enemy broke through to agedabia, benghazi and everything west of tobruk were imperilled.

    如果敵人突破阿傑達比亞，那麼班加西以及托卜魯克以西的一切據點便都岌岌可危了。
  2. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  3. Nigeria have indefinitely suspended chelsea star john obi mikel from playing for them

    奈及利亞國家隊無限期的禁止切爾西球星米克爾參加比賽。
  4. Here we have the first term of a progression, by which the remaining terms are determined with mathematical exactness. the french army went on melting away and disappearing in the same ratio from moscow to vyazma, from vyazma to smolensk, from smolensk to the berezina, from the berezina to vilna, apart from the greater or less degree of cold, the pursuit and barring of the way, and all other conditions taken separately

    從莫斯科到維亞濟馬，從維亞濟馬到斯摩棱斯克，從斯摩棱斯克到別列濟納，從別列濟納到維也納，法軍就是按照上述比例減員和毀滅的，法軍的減員和毀滅與天氣寒冷的程度追擊道路阻障以及一切其他的條件無關。
  5. All of which left a starting unit of kobe, derek fisher, luke walton, turiaf and chris mihm and coach phil jackson understandably announcing he ' d be going with a 10 - deep rotation in the early going

    所有的這一切使得球隊最後只剩下科比、德里克?費舍兒、盧克?沃頓、圖里亞夫和克里斯?米姆，所以教練菲爾?傑克遜宣布說早期他會採用10人輪換的訓練方式，這也是可以理解的。
  6. Chelsea showed resilience to beat valencia in the champions league quarter - finals in midweek and mikel expects the same attitude to prevail against liverpool

    切爾西在周中淘汰瓦倫西亞的冠軍杯1 / 4決賽中展示了他們的韌勁，米克爾希望這種比賽態度能再次擊敗利物浦。
  7. At chelsea, jose mourinho confirms in different interviews that ashley cole and claude makelele will be rested at home to levski sofia next tuesday

    在切爾西，穆帥在接受采訪時指出， a科爾和馬克萊萊不會在下周二與索非亞的比賽中出場。
  8. Chelsea midfielder john obi mikel is in the middle of a row with nigeria coach austin eguavoen

    切爾西的中場小將約翰?奧比?米克爾惹惱了奈及利亞隊主教練奧斯丁?伊加沃恩。
  9. In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals

    首先，用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上，並轉化e colidh5a感受態細胞中，經pcr 、酶切以及測序證明得到了完整的2c3abc基因，並與國內外參考序列進行比較分析。然後，將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ，以zeocin ~ （ tm ）抗性篩選陽性克隆，大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞，通過zeocin ~ （ tm ）抗生素梯度濃度篩選，獲得重組酵母用0 . 5甲醇誘導表達，通過sds - page電泳、 westernblotting分析，結果表明， 2c3abc基因在畢赤酵母中成功表達，其表達產物為一95ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。
  10. Hiddink expects tim cahill to overcome a knee injury in time for the opener against japan on june 12 but said he was disappointed that the injured harry kewell had not been able to join his team - mates yet. " he kewell can be fit just in time for the first game but what we are doing now is to build up the focus and working on tactics and strategy, " he said

    效力于英超埃弗頓的中場球員卡西爾和效力于利物浦隊的前鋒凱維爾目前都有傷在身，雖然如果一切正常的話，他們都有希望趕上6月12日澳大利亞同日本隊的首場比賽，但讓希丁克擔心的是屆時全隊已經失去了最寶貴的演練戰術的時間。
  11. Chelsea defender john terry is a doubt for england ' s euro 2008 qualifiers against fyr macedonia and croatia after missing the blues ' 2 - 0 win against fulham on saturday with a back injury

    切爾西后衛約翰特里，由於背傷，錯過了周六藍軍2 ： 0客場戰勝福勒姆比賽。他能否參見英格蘭2008歐錦賽最戰馬其頓和克羅埃西亞的比賽，還尚未可知。
  12. Chelsea football chief frank arnesen was in the stands to see roysten drenthe star in holland u21 ' s european championship final victory over serbia

    切爾西首席球探佛蘭克.阿內森在看臺上觀看了歐洲u21錦標賽荷蘭戰勝塞爾維亞的決賽，並在比賽中關注了潛力新星德倫特
  13. The former lazio boss has already snapped up rolando bianchi and valeri bojinov, while he had bids for juventus defender giorgio chiellini and palermo midfielder mark bresciano turned down

    這位前拉齊奧主帥已經簽下了羅蘭多?比安奇和瓦勒里?博伊諾夫，同時他又在引進尤文後衛吉奧格里奧?切里尼和巴勒莫中場馬克?布雷西亞諾。
  14. All our manuvres are unnecessary ; that everything is being done of itself better than we could desire ; that we must give the enemy a golden bridge ; that the battles of tarutino, of vyazma, and of krasnoe, were none of them necessary ; that we must keep some men to reach the frontier with ; that he wouldnt give one russian for ten frenchmen. and he, this intriguing courtier, as we are told, who lied to araktcheev to propitiate the tsar, he alone dared to face the tsars displeasure by telling him at vilna that

    他在答復洛里斯頓建議和談時說，不能和談，因為這是人民的意志在法國人退卻時，又是只有他一個人說，我軍的一切調動都沒有必要，一切都聽其自然，這樣會比我們所期望的完成的會更好，對敵人要給以生絡，塔魯丁諾維亞濟馬克拉斯諾耶等戰役，都沒有必要，在抵達國境線時應當還有一點實力，用十個法國人換一個俄國人，他都不幹。
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