比達隆 的英文怎麼說
中文拼音 [bǐdálōng]
比達隆
英文
bidalon-
The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss
將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。Opium, morphine, heroin, methadone, narcotic analgesics, cannabis, lsd, cocaine, ketamine, gamma hydroxybutyric acid ( ghb ), amphetamine ( e. g. methylamphetamine, ecstasy, phentermine ( duromine ) ), benzodiazepine ( e. g. chlordiazepoxide ( librium ), midazolam ( dormicum ), estazolam, flunitrazepam ( rohypnol ), triazolam, nimetazepam, diazepam ( valium ) ), zopiclone, barbiturate ( e. g. quinalbarbitone ), methaqualone ( mandrax ), hallucinogen, depressant, stimulant or tranquillizer
（俗稱綠豆仔） 、咪達唑侖（俗稱藍精靈） 、舒樂安定、氟硝西泮（俗稱十字架） 、三唑侖、硝甲西泮、安定（俗稱羅氏五號、羅氏十號） ） 、佐匹克隆、巴比士酸鹽（如速可巴比妥、甲?酮（俗稱忽得） ）及其他迷幻劑、鎮抑劑、興奮劑及鎮靜劑。In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。In many other countries, where english is not a first language, it is an official language ; these countries include cameroon, fiji, the federated states of micronesia, ghana, gambia, india, kiribati, lesotho, liberia, kenya, namibia, nigeria, malta, the marshall islands, pakistan, papua new guinea, the philippines, rwanda, the solomon islands, samoa, sierra leone, sri lanka, swaziland, tanzania, zambia and zimbabwe
英語在許多國家不是第一語言，但卻作為官方語言存在；這些國家包括：喀麥隆、斐濟、密克羅尼西亞聯邦、迦納、甘比亞、印度、吉里巴斯、賴索托、賴比瑞亞、肯尼亞、納米比亞、奈及利亞、馬耳他、馬歇爾群島、巴基斯坦、巴布亞新幾內亞、菲律賓、盧安達、索羅門群島、薩摩亞群島、獅子山、斯里蘭卡、史瓦濟蘭、坦尚尼亞、尚比亞和辛巴威。Bildad had halted upon the shoulder of the first mountain spur.
比爾達把馬車停在第一道山脊的隆起部。This was the view across the canyon, showing just a small part of the other half of ronda. actually ronda is bigger than one might think
這是穿過峽谷對面的景色，也是隆達另外一半的一小部分。事實上該鎮要比想象的大些。Based on the known result, the cdna of papain was tried to cloned from total rna of papaya in this study. though the primers were designed according to the cdna of papain, the cdna of ppiv was attained, which was homologous to that of papin. when compared with the papain gene in embl database, the cdna of ppiv reachs 98. 7 % in homology
木瓜青果乳汁中含有豐富的半胱氨酸類蛋白酶，據已知的研究結果，本研究嘗試從未成熟的木瓜果的總rna中克隆木瓜蛋白酶基因，雖然在rt - pcr時使用引物的是根據木瓜蛋白酶的cdna序列而設計的，但卻得到了與之有67同源性的番木瓜蛋白酶的cdna序列，對其分析表明，該序列與embldatabase中x78056同一段基因序列相比較同源性達98 . 7 。Hieron won this race b. c. 472, while at the height of his power at syracuse. probably the ode was sung at syracuse, perhaps, as has been suggested, at a banquet
西隆在公元前472的比賽中贏得這次比賽，同時也達到他在錫拉庫扎權力的頂峰。這首頌歌也許是在錫拉庫扎吟唱，也許象曾經說的那樣是在宴會上演唱。In this paper, phylogenetic relationship of 13 species involved in 6 genera of cruciferae wer e carried out through both the clones of homologous sequences with the primers designed on the basis of conserved regions of cyp86mf gene in cytochrome p450 gene superfamily and the differential analyses of them. meanwhile, complete sequences of some genes in cytochrome p450 gene superfamily were isolated and identified by smart pcr - race strategy, and expressed in e. colt. the results were as follows : ( 1 ) isolated by pcr from 11 species of cruciferae, eleven homologous gene segments that deduced amino acids were identities of over 80 % at nucleotide sequence level and similarities of over 70 % at amino acid sequence level
本論文以已知的細胞色素p450基因超家族成員cyp86mf基因的保守區設計引物對十字花科重要蔬菜作物的6個屬13個物種進行了同源序列的分離克隆，通過核酸序列的差異比較分析，研究了該基因在不同物種中的進化關系；同時，通過保守引物的pcr擴增和race相結合的方法對十字花科植物不同物種的細胞色素p450基因家族成員基因全長進行了分離克隆、鑒定和原核表達的研究，獲得如下研究結果： （ 1 ）通過pcr從十字花科植物不同物種中擴增到11個可以推導出完整氨基酸序列的同源片段。As a result, the provinces all prospered in his reign, informers were abolished, the confiscation of goods was less frequent than ever before, and only one man was condemned as guilty of aspiring to the throne
結果，所有的省都在他執政期間興隆發達，告密被廢除，沒收貨物的事情比以前任何時候都要少，只有一個人因為想篡位被判刑。Then daguenet added a rapid word or two about the rest. there was clarisse besnus, whom a lady had brought up from saint - aubin - sur - mer in the capacity of maid while the lady s husband had started her in quite another line. there was simonne cabiroche, the daughter of a furniture dealer in the faubourg saint - antoine, who had been educated in a large boarding school with a view to becoming a governess. finally there were maria blond and louise violaine and lea de horn, who had all shot up to woman s estate on the pavements of paris, not to mention tatan nene, who had herded cows in champagne till she was twenty
隨后，其餘女人的情況達蓋內就三言兩語地說一下：克拉利瑟貝尼，是被一個太太從海濱聖歐班帶來作女僕的，后來那個太太的丈夫把她送出來當了煙花女西蒙娜卡比羅什是聖安托萬郊區的一個傢具商的女兒，在一所很大的培養小學教員的寄宿學校里長大瑪麗亞布隆路易絲維奧萊納和萊婭德霍恩都是被迫走上巴黎街頭，淪為娼妓的。Quality besides micronaire value and elongation of relevance not significant, other quality traits of both showed significant level ; yield and quality traits associated single boll weight and the first half of the average length uniformity index significantly related, and the breaking strength of correlation was significant, and micronaire value was negatively correlated ; lint and micronaire value elongation reached significant levels and a very significant level, and on average half the length uniformity index, breaking strength of correlation was significant but was negatively correlated
品質性狀間除了麥克隆值與伸長率的相關性不顯著之外，其餘品質性狀間均呈現出極顯著水平；產量性狀與品質性狀的相關性中單鈴重與上半部平均長度、整齊度指數相關性顯著，與斷裂比強度相關性極顯著，與麥克隆值呈負相關；衣分與麥克隆值、伸長率分別達到顯著水平和極顯著水平，與上半部平均長度、整齊度指數、斷裂比強度等相關性極顯著但呈負相關。The characteristics of quantum computing and the mechanism of immune evolution are analyzed and discussed. inspired by the mechanism in which immune cell can gradually accomplish affinity maturation during the self - evolution process, a immune evolutionary algorithm based on quantum computing ( mqea ) is proposed. the algorithm can find out optimal solution by the mechanism in which antibody can be clone selected, memory cells can be produced, similar antibodies can be suppressed and immune cell can be expressed as quantum bit ( q - bit ). it not only can maintain quite nicely the population diversity than the classical evolutionary algorithm, but also can help to accelerate the convergence speed and converge to the global optimal solution rapidly. the convergence of the mqea is proved and its superiority is shown by some simulation experiments in this paper
分析和探討了量子計算的特點及免疫進化機制，並結合免疫系統的動力學模型和免疫細胞在自我進化中的親和度成熟機理，提出了一種基於量子計算的免疫進化演算法.該演算法使用量子比特表達染色體，通過免疫克隆、記憶細胞產生和抗體相似性抑制等進化機制可最終找出最優解，它比傳統的量子進化演算法具有更好的種群多樣性、更快的收斂速度和全局尋優能力.在此不僅從理論上證明了該演算法的收斂，而且通過模擬實驗表明了該演算法的優越性Abstract : the characteristics of quantum computing and the mechanism of immune evolution are analyzed and discussed. inspired by the mechanism in which immune cell can gradually accomplish affinity maturation during the self - evolution process, a immune evolutionary algorithm based on quantum computing ( mqea ) is proposed. the algorithm can find out optimal solution by the mechanism in which antibody can be clone selected, memory cells can be produced, similar antibodies can be suppressed and immune cell can be expressed as quantum bit ( q - bit ). it not only can maintain quite nicely the population diversity than the classical evolutionary algorithm, but also can help to accelerate the convergence speed and converge to the global optimal solution rapidly. the convergence of the mqea is proved and its superiority is shown by some simulation experiments in this paper
文摘:分析和探討了量子計算的特點及免疫進化機制，並結合免疫系統的動力學模型和免疫細胞在自我進化中的親和度成熟機理，提出了一種基於量子計算的免疫進化演算法.該演算法使用量子比特表達染色體，通過免疫克隆、記憶細胞產生和抗體相似性抑制等進化機制可最終找出最優解，它比傳統的量子進化演算法具有更好的種群多樣性、更快的收斂速度和全局尋優能力.在此不僅從理論上證明了該演算法的收斂，而且通過模擬實驗表明了該演算法的優越性Acetylornithine deacetylase is the key enzyme of producting l - methionine. we mainly do research work on the construction of acetylornithine deacetylase gene - engineering strain and characteristic of proteinase. in order to get high expression deacetylase strain, we obtain the gene by pcr arge gene. the product ( 2800bp ) was cloned into puc19 plasmid and confirmed with blue / white dot screening > restriction enzyme analysis and pcr. then taking the nucleotide sequencing compared with the sequence at blast of u. s. a. we constructed a high expression of gene - engineering strain - pxj 128 which containing the arge gene on the high expressing system of pxji18 with activity of acetylornithine deacetylase above 20000u / g
為了獲得高效表達的脫乙酰鳥氨酸酶工程菌株，在工程菌技術改造及其固定化研究做了進一步的研究和探討。我們採用基因工程技術，通過pcr技術擴增出了酰化酶關鍵酶基因?脫乙酰鳥氨酸酶基因arge ，將其克隆到puc19載體中，經酶切鑒定、 pcr鑒定篩選出重組陽性質粒，並測序鑒定，通過美國blast程序進行了基因數據庫相似性比較分析。After our beautiful and gracious supreme master ching hai lectured in kuala lumpur, malaysia, on april 30, 2000, local initiates were filled with happiness and overflowing with love. our enthusiasm to share master s teachings could not be contained, and so with a quan yin messenger, malaysian initiates presented six video seminars in the major cities of malaysia, namely, perak, kadah, ipoh, butterworth, malacca, and penang
自從我們美麗優雅的清海師父在2000年4月30日蒞臨馬來西亞吉隆坡講經之後，同修們法喜充滿愛力洋溢，熱切地想要分享師父教理給更多人，於是與觀音使者一起在馬來西亞各主要城市舉辦了六場錄影帶講座，這些城市包括比叻卡達怡保巴特渥斯麻六甲與檳城。Representatives from 17 african countries were present in the chinese capital ? benin, congo, egypt, mali, kenya, south africa, nigeria, sudan, ethiopia, rwanda, algeria, mozambique, tanzania, djibouti, mauritania, cameroon and senegal
來自非洲17個國家的代表出席了會議：貝南、剛果、埃及、馬里、肯尼亞、南非、奈及利亞、蘇丹、衣索比亞、盧安達、阿爾及利亞、莫三比克、坦尚尼亞、吉布地、毛利塔尼亞、喀麥隆和塞內加爾。Among sequenced 16 positive clones randomly selected, two represent novel expression tag sequences, two are homologous to two unknown proteins in genbank ; the rest are homologous to known or putative proteins or enzymes with definite functions by searching the genbank through blast program, which are involved in various life activities of cell such as regulation of gene expression, plant secondary metabolism, signal transduction, adversity resistance, stress response and defense reaction. significant changes of quantities of these gene fragments were observed before and after ssh, which indicated they were enriched after ssh
隨機挑選了16個差異表達的克隆進行序列測定，經與genbank數據庫相關數據比較分析，發現有2個新的cdna片段（ ets ） ，有12序列與genbank中已知蛋白或推測蛋白質（ putativeprotein ）序列有高低不同的同源性，它們參與基因的表達調控、植物的次生代謝、信號傳導、抗逆、應激及防禦反應等細胞生命活動過程。If you come from such countries as afghanistan, nigeria, ghana, bangladesh, somalia, cameroon, liberia, uganda, palestine, and have hong kong id card, you may apply for on - site visa at the customs ; in case you have no id card, you have to apply for a visa at hong kong ministry of foreign affairs
國外參展商和專業觀眾如來自國家為：阿富漢、奈及利亞、迦納、孟加拉國、索馬里、喀麥隆、賴比瑞亞、烏干達、巴勒斯坦，如有香港身份證，可以直接在入境海關關口辦理落地簽證（指在入境關口簽證） ，否則，也需要到香港外交部或本國辦理簽證。Hisityl - trna synthetase catalyzes the aminoacylation of trnahis in the initial step of protein biosynthesis. the involumen of histidyl - trna synthetase in autoimmune diseases is another feature of the enzyme. in studies, it is reported that purified jo - 1 antigen can increase the detection rate of anti - jo - 1 antibody. but it is difficult to obtain a single component by biochemical extraction. genetic engineering can help us to desolve this problem. after looking up mrna sequence encoding histidyl - trna synthetase in genebank, we used rt - pcr technology to gain its full length dna sequence. the vestor ptybllwas used in the construction of expressing vestor. we transformed the jo - 1 gene into er2566 and used this system to express fusion jo - 1 antigen
本實驗從人胎盤中提取總rna ，利用rt - pcr技術獲得了編碼jo - 1的基因整長序列，選用impact - cn系統中的ptyb11載體，構建了jo - 1基因的克隆與表達載體，並轉化大腸桿菌er2566 ，經過抗性篩選、分子量大小比較、雙酶切鑒定、和pcr鑒定等多種方法驗證，篩選出了5個陽性克隆。分享友人