氨克生 的英文怎麼說

中文拼音 [ānkèshēng]
氨克生 英文
mtx
  • 氨 : 名詞[化學] （氮和氫的化合物） ammonia; hydrogen nitride
  • 克 : 克i 動詞1 （能） can; be able to 2 （克服; 克制） restrain; control 3 （攻下據點; 戰勝） overcome...
  • 生 : Ⅰ動詞1 （生育; 生殖） give birth to; bear 2 （出生） be born 3 （生長） grow 4 （生存; 活） live;...
  1. Furbenicillin sodium, a known semi - synthetic ureide type of antibiotic firstly marketed in china, was synthesized from starting material 2 - furaldehyde via six steps including oximation reaction, beckmann rearrangement, isocyannuration, amination, condensation and salification etc

    摘要以呋喃甲醛為起始原料，經厲化、貝克曼重排、異氰脲酸化、氨基化、縮合和成鹽6步反應，合成了酰脲類青霉素抗生素呋布西林鈉。
  2. Based on the previous studies, the research in this paper was carried out, mainly including two parts as follows : ( 1 ) anammox bacteria and aerobic ammonia oxidizers were detected in situ in 6 sediment samples taken from jiangsu province. molecular techniques, such as fish, pcr, dna cloning and sequencing etc. were used for this purpose. ( 2 ) the continuous cultivation of anammox bacteria from sediment samples were studied, which provides experimental basis for the bioaugamentation of eutrophicated sediment applying anammox process

    本論文在前人研究的基礎上，開展了以下兩個方面的工作： （ 1 ）採用分子生物學技術熒光原位雜交（ fish ） 、多聚酶鏈式反應（ pcr ） 、 dna克隆和測序等對采自江蘇省蘇州市、東太湖、新沂河等6個底質樣品進行了厭氧氨氧化菌和傳統氨氧化菌的原位檢測； （ 2 ）探討了以底質作為接種體進行厭氧氨氧化菌富集培養的可行性，為天然底質環境中厭氧氨氧化過程的強化，富營養化底質微生物修復的可行性提供一定的依據。
  3. At first, 1. 67 u g per well mcab all was coated on three wells of a plate, and then 1. 5 x 1011 phage virion was diluted and added, after incubating with the target, wash away unbound phage by tbst ( 0. 1 % tween - 20 ), the bound phage was eluted with ph 2. 2 tris - gly buffer and amplified, the specially bound phage was enriched by taking through addition binding / amplification cycles. ln the following cycles, the stringency of panning can be increased by raising the concentration of tbst or decreasing that of mcab all, collecting and titering the washing phage of last time and output phage in each round, the selective ratio and the false positive rate of each round were worked out, the gradually increasing of selective ratio and decreasing of positive rate shows that the panning was effective. after 4 rounds of panning, 11 phage clones were selected after competitive - ellsa, the dna samples of 8 positive clones and 1 negative clone were sequenced and all the foreign peptides inserted was also deduced, a clear consensus binding sequence emerged

    在本實驗中，利用隨機12肽庫對抗豬瘟病毒（ classicalswinefeverviruscsfv ）糖蛋白me2的單抗a11進行表位篩選，經過四輪篩選以後，隨機挑取11個克隆作競爭- elisa檢測，結果表明，所挑11個克隆中，有9個克隆能對me2蛋白和a11反應產生抑制作用，抑制率最高可達64 ； dna測序以後經過dnastar軟體分析，發現它們的核心序列為anwralsl ，該核心序列與豬瘟病毒e2蛋白的28 - 35位氨基酸ttwkeysh具有同源性；夾心- elisa檢測和western - blotting試驗均證明所挑陽性克隆能被a11所識別；人工合成含核心序列的多肽經間接elisa試驗證實，也能被a11識別。
  4. The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins

    將帶有hwtx -基因的ppic9k經blgii線性化后，轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌，進一步用遺傳毒素g418篩選多拷貝的轉化菌株，命名為gh1 ；將gh1甲醇酵母菌用0 . 5的甲醇誘導表達，發酵上清經90飽和度的（ nh _ 4 ） _ 2so _ 4沉澱， yw - 3 （ mwc03000 ）的超濾膜超濾，再經cm陽離子交換， c _ （ 18 ）反相hplc純化得到分子量為4kd左右的組分，其中4289 . 05的組分經質譜鑒定，氨基酸組成分析和序列測定為正確的表達產物，生物學活性表明其活性為天然毒素活性70 % ，表達量為80mg / l 。
  5. In order to check if it is the aim gene, we devised pcr with a new pair of primer, sequenced and registered the product with registration number : af449446. moreover we forecast and analysis the primary, secondary, tertiary and quaternary structure of the three protein : osftszi, crftszi and crftsz2 which has already cloned by our team before. after that we construct ftsz molecular evolution tree to site them in

    又將生物信息學技術同實驗技術相結合，針對ftsz保守區設計引物擴增出一條衣藻ftsz片段，進行est搜索、比對、拼接，最終克隆出新基因crftsz1 ；連同本試驗室曾經獲得的另一個衣藻crftsz2基因進行蛋白質的一、二、三、四級結構預測、分析及比較尋找進化線索，建立了ftsz蛋白的氨基酸進化樹作進一步的進化定位。
  6. Besides, parker is also a famous supplier for refrigeration and air conditioning components including all kinds of sporlan products, such as mechanical and electric expansion valves, solenoid valves, filter driers, sight glasses, level master liquid controls, mechanical and electric pressure regulating valves, check valves, shut off valves, oil separators, and other industrial valves for r717 etc

    除此之外，派克漢尼汾還是一家全球著名的冷凍與空調另部件的供應商，其中包括美國斯坡蘭生產的各種產品，如，機械的和電子的膨脹閥，電磁閥，乾燥過濾器，視鏡，液位控制器，機械的和電子的壓力調節閥，單向閥，截止閥，油分及工業用氨閥等等。
  7. One group was told they ' d be watching a funny movie, the other was not. " blood drawn from experimental subjects just before they watched the video had 27 percent more beta - endorphins and 87 percent more human growth hormone, compared to blood from the control group, which did n ' t anticipate the watching of a humorous video, " explained lee berk of loma linda university

    負責此項研究的心理學家李伯克說： 「血液抽樣調查顯示，與第二組不知道要看電影的人相比，第一組被試在看電影之前，血液中氨多酚一種會使人愉快的物質的含量高出27 ，生長激素含量高出87 。 」
  8. To prepare the wild type mbl in prokaryotic system, a pair of primers was designed and synthesized, and was used to amplify mbl gene from the recombinant vector pgem - mbl that contans wild type mbl cdna. a recombinant prokaryotic expression vector, pet28 - mbl, was constructed by inserted the mbl gene into plasmid pet28 ( b ), and after transfected it into ecoli bl21 ( de3 ) and induced with iptg, recombinant mbl protein was expressed successfully

    本實驗另選用了原核表達質粒pet28 （ b ） ，根據已構建好的含有mbl野生型基因的t載體pgem - mbl ，設計一對引物， pcr擴增mbl基因，凝膠回收，雙酶切pcr產物和pet28 （ b ）質粒， t4連接酶連接，轉化大腸桿菌dhsa ，氨芋選擇培養挑取克隆鑒定。
  9. The amplified cdna fragment was inserted to plasmid puc 18. the sequencing result shows that length of cgh cdn a fragment is 669bp

    正反向測序結果分析表明：克隆的草魚生長激素基因cdna全長669bp ，開放閱讀框（ orf ）包含633個堿基，編碼210個氨基酸。
  10. Sequence analysis indicated that the opening reading frame of ns1 gene is 1056nt in length, encoding 352 amino acids, identical to the sequence of strain sa 14 - 14 - 2 deposited in genbank. ns1 gene in the pmd18 - t - ns1 was cut by bamhi and hindiii, was cloned into the expression plasmid pet - 30b ( + ) treated with the same enzymes, resulting in a recombinant plasmid pet - 30b - ns1. the pet - 30b - ns1 was identified by pcr, restriction digestion, and sequencing

    通過序列分析結果表明， jev - ns1基因編碼區核苷酸序列長度為1056bp ，編碼352個氨基酸，序列分析和比較表明本實驗克隆到的ns1蛋白基因與genbank中收錄的疫苗株sa14 - 14 - 2的核苷酸序列一致，表明盡管該疫苗株在我國應用多年，但ns1基因並未發生變異，提示該疫苗株遺傳性狀比較穩定，是一株優良的疫苗株。
  11. Beth showed 56 % identity to the opud of bacillus subtilis and belonged to bcct family. its putative promoter region was highly homologous to b - dependent promoter of b. subtilis. a 2. 6 kb fragment including the beth gene was subcloned into puc18 and transformed into the e. coli mkh13, colonies appeared on the plate of the selective m9 medium

    將包括整個beth基因orf框及可能的啟動子核苷酸序列在內的2 . 6kb的片段克隆到puc18載體上，轉入到大腸桿菌甘氨酸甜菜堿缺失株mkh13中，使該菌株能夠在含甘氨酸甜菜堿的高鹽m9培養基上生長，而對照實驗不能生長。
  12. In this dissertation, the following experiments are conducted : firstly, cloning of hpab - gene, i ) the amino acids sequences of 23 animal - peptide antibiotics were analyzed by multiple sequences alignment method and a pair of degenerated primers were designed according to the consensus sequence derived from mature peptides of some beta peptide antibiotics

    人源肽抗生素hpab -的基因克隆：對動物23種型肽抗生素的氨基酸序列進行對比，選擇部分相似性較高的序列，分析其成熟肽的結構特點，抽提型肽抗生素的一級結構特徵，並以此為基礎設計簡並引物。
  13. Fr - 008 and streptomyces griseus imru3570 are two independently isolated producers for a similar heptaene macrolide antibiotic. comparative studies between the two producers at chemical and biological level are the main task of the present work, no obvious difference was observed on the hplc profiles

    本文主要從化學和生物學兩個角度分析和比較了這兩個菌株的異同，並對抗生素的氨基海藻糖殘基基因進行了克隆嘗試。
  14. It possess high homology with those of ck2 ( casein kinase 2 ) a subunits from various species. it is now well established that protein kinase ck2 is a pleiotropic and abiquitous serine or threonine kinase, which is highly conserved during evolution. we obtained the complete cdna sequence of ck2 a via 5 " race

    酪蛋白激酶ck2是目前發現的普遍存在於真核生物中的絲氨酸蘇氨酸型蛋白激酶，進化上具有高度的保守性並參與了多種生理功能的調控，因而克隆它的基因具有重要意義。
  15. Recently, a noval brain - specific na ^ - dependent inorganic phosphate transporters was successfully cloned using the method of molecular biology and was named dnpi because they had the characters of the vesicular glutamate transporter ( vglut ). some studies showed that dnpi were distributed extensively in the brain area where glu was used as the neurotransmitter, such as thalamus, medulla oblongata and spinal cord. the ultrastructural studies indicated that most of dnpi are located in the presynaptic terminals and distributed on the synaptic vesicular membrane

    最近人們應用分子生物學技術又成功地在哺乳類動物腦內克隆出一種新的腦內特殊的na 」依賴的無機磷酸轉運體（ brain七pecificna dependentinorgarucphosphaternsporter ， bnpi ） ，並發現它也具有囊泡膜谷氨酸轉運體（ vglllt ）的特點，因而被命名為dnpi （又稱作vgiutz人新近的研究報道， dn 』 pi廣泛地分佈於運用m作為神經遞質的腦區，如丘腦、延髓和脊髓。
  16. Its gene includes seven exons ( 3 - 9 ) and six introns ( 3 - 8 ). with respect to zfghrl, it is also like other ghr1s, which consists of 1578bp and encode a 528 amino acid protein. its gene only includes eight exons ( 1 - 5, 8 ) and seven introns ( l - 5 ). various methods used to find out the transmembrane and boxl domain of zfghrl but the result turns out to be fruitless due to unknown reason. fghr2 and zfghr2 : fghr2 includes eight exons and seven introns, which consists of 1710 bp and encodes a 569 amino acid protein ; zfghr2 includes eight exons ( 1 - 5, 8 ) and seven introns ( 1 - 5 ), which consists of 1758 bp and encodes a 484 amino acid protein

    得到的fghr1含有7個外顯子（ 3 - 9 ）和6個內含子（ 3 - 8 ） ，由1755個bp的核苷酸組成並編碼一個584個氨基酸殘基的蛋白質：得到的zfghr1包含有1587個核苷酸並編碼一個528個氨基酸殘基的蛋白質，理學碩士學位論文:南方貼生長激素受體cdna的分子克隆和魚類存在兩種生長激素受體的證明其基因只有8個外顯子（ l一5 ， 8 ）和7個內含子（ 1一5 ） 。
  17. It was very impossible that those mutations of gx epidemic strains of csfv can cause the incompleted protection of hclv vaccine

    基因的克隆與序列分2729 、 734和738位氨基酸均發生了變異。
  18. In this study, the complete cdna of resveratrol synthase was cloned from peanut by rt - pcr. two plant constitutive expression vectors under the control of camv35s promoter were constructed by inserting the rscdna into pcambia1301 - pt 4a

    本研究利用rt - pcr方法從花生中克隆得到rscdna ，並且測序驗證與已知序列同源性達到99 % ，氨基酸序列完全相同。
  19. Yellow powder insect ' s doesing an article is to use yellow powder insect to process with meticulous care, it measure chemical element including phosphotus, potassium, iron, sodium, aluminum etc. and various little chemical elements and animal growth 16 kinds of essential amino acidses, each 100 gram stem the article contain amino to be up to 847. 91 milligrams, various nourishment composition resides each kind of head of animal feed

    黃粉蟲干品是我基地優良黃粉蟲精心加工而成，內含有磷、鉀、鈉、鋁等常量元素和多種微量元素及動物生長所必須的16種氨基酸，每100克干品含氨基酸高達847 . 91毫克，各種營養成份居各類飼料之首。
  20. The activity of the invertase of three high - sugar saccharomyces cerevisiae strains were investigated and their amino acid sequence were determined by the use of modern biotech including enzymatic activity determination, specific pcr, the cloning sequencing and bio - information analysis etc

    摘要採用現代生物技術中的酶活測定、特異pcr 、克隆測序和生物信息分析等對比研究3株高糖酵母蔗糖轉換酶活性及其氨基酸序列。
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