氨基磷酸酶 的英文怎麼說
中文拼音 [ānjīlīnsuān]
氨基磷酸酶
英文
phosphaminase-
The enzyme 5 - enolpyuvyl - 3 - phosphoshikimic acid synthase ( epsp synthase ; ec2. 5. 1. 19 ), encoded by anoa locus, is a key enzyme present in microorganisms and plants where it has a function in the biosynthesis of aromatic amino acids. glyphosate ( n - phosphonomethyl - glycine ) is an effective non - selective, broad spectrum, postemergence herbicide, which has been shown to inhibit epsp synthase activity in a competitive manner. glyphosate tolerant plants can be mediated by either overproduction of the target enzyme or by the presence of an altered enzyme
植物和微生物芳香族氨基酸生物合成過程中的一個關鍵酶? ? 5 -烯醇丙酮莽草酸- 3 -磷酸合成酶( epspsynthase ; ec2 . 5 . 1 . 19 )由aroa基因編碼,該酶受廣譜滅生性、內吸傳導型除草劑草甘膦的競爭性抑制,將epsp合成酶基因轉入植物中可獲得草甘膦耐受植株。This is catalyzed by a transaminase enzyme in conjunction with the coenzyme pyridoxal phosphate
催化這個反應的酶是氨基轉移酶,其上連接有磷酸吡哆醛作為輔酶。The amino group becomes attached to the coenzyme to form pyridoxamine phosphate, and is then transferred to the a - keto acid, which is usually pyruvic acid, oxaloacetic acid, or - ketoglutaric acid
反應中氨基首先連接在輔酶上形成磷酸吡哆氨,然後氨基轉移到-酮酸上,這種-酮酸可以為丙酮酸、草酰乙酸或是-酮戊二酸。The stations e2 and 1 - 4 were located at the cold water mass area of the central yellow sea, which characterized by low temperature, high salinity and stable theromocline would generate a retention mechanism that promoted the formation of separate, self - supporting stocks of krill. 2 genetic diversity and differentiation of p. latifrons specimens of p. latifrons were collected from the east china sea and the south china sea. the zymogram phenotypes of aspartate aminotransferase ( e. c. 2. 6. 1. 1, aat ), alkaline phosphatase ( e. c. 3. 1. 3. 1, alp ), a - amylase ( a - amy ), r - amylase ( r - amy ), esterase ( est ), lactate dehydrogenase ( ldh ), raalate dehydrogenase ( mdh ), malic enzyme ( me ), and phosphoglweoisomerase ( pgi ) were scored
(二)寬額假磷蝦遺傳多樣性和遺傳分化研究1 .本文對東海外海和南海2個站位寬額假磷蝦群體進行了分析,在檢測的9個酶系統中,共檢測到11個酶位點:天冬氨酸轉氨酶( l個位點, 2個等位基因) ,堿性磷酸酶( 2個位點, a加了和a加2各有2個等位基因) , r澱粉酶( l個位點, 2個等位基因) ,醋酶( 2個位點, es巧和est7各有2個等位基因) ,蘋果酸脫氫酶( l個位點, 3個等位基因) ,蘋果酸酶( l個位點, 2個等位基因) ,乳酸脫氫酶( l個位點, 4個等位基因) ,磷酸葡萄糖轉氨酶( l個位點, 3個等位基因) ; a澱粉酶為單態。The enzyme digest analysis shows that the arm repeats of c - terminal are conceivably conservative domain. in arc1 protein, there are some active sites including n - glycosylation sites, camp - and cgmp - dependent protein kinase phosphorylation sites, protein kinase c phosphorylation sites, casein kinase ii phosphorylation sites, tyrosine kinase phosphorylation sites, n - myristoylation sites, amidation sites and leucine zipper pattern. it probably take part in the signaling process of self - incompatibility
同時在arc1蛋白質中還發現了拉鏈結構和多個磷酸化位點,包括camp和cgmp依賴的蛋白激酶磷酸化位點、蛋白激酶c磷酸化位點、酪蛋白激酶磷酸化位點、酪氨酸激酶磷酸化位點、糖基化位點等,拉鏈結構為arc1蛋白之間及與其它蛋白的相互作用提供了可能,而磷酸化位點是arc1參與信號傳導過程所必需的。Northern blot results show that nos. 66 - 1, 84, 89 - 1, 97, 108, 152, 175 and 233 have stronger signal in sp6 - tester than in sp6 - driver ; and no. 23 has weak signal only in sp6 - tester, nos. 94, 165, 172, 185 and 191 have similar hybridization signals in both sp6 - tester and sp6 - driver ; nos. 4, 17, 18, 28, 6 9, 101, 156 - 1, 157 - 1 and 183 do not reveal hybridization signals in both sp6 - tester and sp6 - driver ; the results of sequencing and blastn and blastx on ncbi indicate that no. 23 cdna ( 846bp ) has significant alignments with nicotiana tabacum mrna for elicitor inducible beta - 1 - glucanase nt - sube76, and arabidopsis thaliana clone 7119 for glycosyl hydrolase family 17 ( protein id : at5g55180. 1, supported by cdna : 7119, supported by cdna : gi _ l 87001 54 ) and arabidopsis thaliana beta - 1 - glucanase - like protein ( gi _ 2 1594590 ) ; no. 84 cdna ( 560bp ) has significant alignment with lotus corniculatus aspartate aminotransferase mrna ( complete cds length = 1685, gi | 2605931 | gb | af029898. 1 | af029898 ) for aspartate aminotransferase ; no. 89 - 1 cdna has significant alignment with arabidopsis tha
與同源性最高的擬南芥類似晚期胚胎發生高豐度蛋白比較,二者都具有lea 2結構域、保守分泌蛋白cog5608結構域和低復雜度區,都具有pkc磷酸化位點、酪蛋白激酶磷酸化位點、 n十四酞化位點和酚胺化位點,所不同的是: ( )在結構功能域上, 152全長cdna編碼的蛋白質序列中多了1個lea 2結構域、 l個保守分泌蛋白cog5608結構域和1個低復雜度區; ( 2 )在功能位點上, 152全長cdna編碼的蛋白質具有酪氨酸硫酸化位點、多了l個酪氨酸激酶磷酸化位點和1個可能的天冬氨酸富集區,但沒有n糖基化位點; ( 3 )擬南芥類似晚期胚胎發生高豐度蛋白的lea 2結構域具有顯著性( eTo neutralize the function of cam, the cam binding domain peptide from bovine calcineurin a subunit was chosen because the pre - experiment demonstrated that bovine calcineurin could bind to calmodulin strongely in an acidic and high calcium concentration condition which is similar to the cell wall environment. in order to increase the binding ability of the chimeric secreted calmodulin binding peptide, the plasmid containing two copies of calmodulin binding domain were constructed
它共有26個氨基酸,具有信號肽的典型特徵。為了削弱質外體cam的功能,選擇了牛腦鈣調神經磷酸酶can ( calcineurin )的a亞基的cam結合域。預實驗結果表明,在近似於胞外環境的酸性高鈣條件下can與cam有強結合。The cyst cells enclosing spermatomeres maybe synthesize a kind of scf - like protein, which can recognize specially the c - kit receptor on the cellular membrane of spermatomeres. then c - kit is activated, dimerizing and autophosphorylating. at the same time, the tyrosine kinase domain of c - kit is activized, which phosphorylates the proteins that have sh2 domain
精母細胞周圍的囊細胞可能合成scf樣蛋白,特異地識別精母細胞膜上的c - kit受體,並刺激c - kit發生二聚化、自體磷酸化,激活胞內酪氨酸激酶活性,活化具有「 sh _ 2結構域」的靶蛋白,可能通過一系列信號級聯,最終激活與減數分裂的相關蛋白或基因。Exchangeable aluminium, humic acid component content in rhizosphere soil and non - rhizosphere soil, activity acidity, organic matter content, humus component content, total amount of fifteen types of amino acid, the content of various forms of phosphorus, the amount of three main types of microbes, enzyme activity, and bulk density in non - rhizosphere soil increased
根際和非根際土壤交換性鋁含量、胡敏酸組分,非根際土壤活性酸、有機質含量、腐殖質組成、 15種氨基酸總量、各磷形態含量、三大土壤微生物數量、酶的活性和土壤容重增加。Mongolica young stand, secondary natural forest, and the mixed forest of larix olgensis and fraxinus mandshurica rupr. countermeasures for controlling soil degradation were also recommended. the study got following results : ( 1 ) from young stand to mid - age of larix olgensis, with the closure of canopy and the decrease of undergrowth vegetation cover, soil acidity, enzyme activity, total inorganic phosphorus, available k, soil moisture and total porosity decreased significantly ; while soil organic matter content, humus component content, the amount of three main types of microbes, acid phosphatase activity, and bulk density in rhizosphere soil and non - rhizosphere soil increased with the increase of the amount of forest floor litter ; from mid - age stand to near mature stand, with the decrease of stand density and crown cover as well as the restoration of undergrowth vegetation after preliminary thinning, soil organic matter content, fulvic acid, exchangeable acidity, exchangeable aluminium, neutral phosphatase activity, total amount of inorganic phosphorus, the amount of actinomycetes, soil moisture, and total porosity in rhizosphere soil and non - rhizosphere soil apparently increased ; from near matu re stand to mature stand, soil activity acidity, organic matter content, humus component content, available phosphorus, total amount of amino acid, available k, soil enzyme activity, soil moisture, and total porosity in rhizosphere soil and non - rhizosphere soil decreased, but total phosphorus, soil acidity ( in particular, exchangeable aluminium ), and bulk density increased
結果表明: ( 1 )從落葉松幼齡林到中齡林,隨著林冠的郁閉和林下植被蓋度的急劇下降,林地土壤酸度、土壤酶活性、無機磷總量、速效鉀、土壤水分含量、總孔隙度呈明顯下降趨勢,而根際和非根際土壤有機質含量、腐殖質各組分含量、三大類微生物數量、酸性磷酸酶活性和土壤容重隨著凋落物的增加呈明顯上升趨勢;從中齡林到近熟林,由於撫育間伐,林分密度及郁閉度下降,林下植被蓋度逐步得到恢復,根際與非根際土壤有機質含量、富里酸、交換性酸、交換性鋁、中性磷酸酶、無機磷總量、放線菌數量、土壤水分含量、總孔隙度呈明顯的上升趨勢;從近熟林到成熟林,隨著林齡的增大,根際與非根際土壤活性酸、有機質含量、腐殖質各組分含量、有效磷含量、氨基酸總量、速效鉀、土壤酶的活性、土壤水分含量和總孔隙度呈下降趨勢,而根際與非根際土壤全磷含量、土壤酸度(特別是交換性鋁)和容重呈增加趨勢。Layered and pillared material are a kind of multifunctional material which were developed in recent years, much attention has been paid to this kind of material for its application in ion - exchange catalysts solid state proton conductivity, nonlinear optics and physic. a lot of literature have reported the intercalation behavior of a - zirconium phosphate ( abbreviated as a - zrp ), different guest molecules inserted into a - zrp have been studied in detail, those guest molecules include amine, alcohok amino acid protein, enzyme coornadiate compound and coronal compound. the intercalation guest is restricted by their size and basicity
層柱材料是近年來發展起來的一類多功能材料,由於其在離子交換、催化、固態質子導體、非線性光學以及醫學等方面的廣泛應用而受到國內外研究者的重視,大量文獻報道了-磷酸氫鋯zr ( hpo _ 4 ) _ 2 ? h _ 2o ( - zirconiumphosphate ,縮寫為- zrp )的超分子插層化合物及插層性能,其中對不同的客體分子對磷酸鋯的嵌入做了詳細的報道,客體分子的種類包括氨、醇、氨基酸、蛋白質、酶、配合物、冠狀化合物等。It is divided to extracellular and intracellular part by transmembrane domain. there are 13 n - glycosylation sites, 20 protein kinase c phosphorylation sites, 28 casein kinase ii phosphorylation sites, 4 tyrosine kinase phosphorylation sites and 15 n - myristoylation sites in the extracellular part of bt - r3 protein. an integrin recognition sequences rod lies in intracellular part of bt - r3 protein
跨膜區域( tmd )將它分為胞內和胞外兩個部分,它的胞外有13個潛在的糖基化位點, 20個蛋白激酶c的磷酸化位點, 28個酪蛋白激酶的磷酸化位點, 4個酪氨酸酶的磷酸化位點, 15個豆蔻(十四烷基)酰化位點;它的胞內有1個整合蛋白( integrin )識別位點。The principle the key influential factor of enzyme inhibition spectrophotometry for determination of organophosphate and carbamate pesticide residues in vegetable had been described, and had discussed the insufficient problems such as false positive results, negative enzyme inhibition rate which exists in this enzyme inhibition spectrophotometry and why organophosphate and carbamate pesticide residues in certain vegetables such as leek cannot be determined with this enzyme inhibition spectrophotometry
摘要對利用酶抑制分光光度法檢測蔬菜上有機磷和氨基甲酸酯類農藥殘毒的原理、最主要影響因數和存在的問題進行了探討,重點分析了酶抑制分光光度法出現假陽性、負抑制率以及該方法不適用於檢測韭菜等蔬菜中的有機磷和氨基甲酸酯類農藥殘留的原因。Huangyal4 was complete nucleotide sequence of 1 854 bp with a nucleotide orf ( 1575 bp ), which encoded a protein consisting of 524 aa with molecular weight of 62. 2 kda and pi of 8. 96. strongly basic ( + ) amino acids, strongly acidic ( - ) amino acids, hydrophobic amino acids and polar amino acids of the protein were 13. 74 %, 11. 64 %, 36. 45 % and 22. 70 % respectively, and predicted secondary structure of the protein revealed many conserved domains such as n - glycosylation site, protein kinase c phosphorylation site, casein kinase ii phosphorylation site, n - myristoylation site, camp - and cgmp - dependent protein kinase phosphorylation site, tyrosine kinase phosphorylation site and a cytochrome p450 cysteine heme - iron ligand signature which was typical of cytochrome p450. a - helix and b - sheet of the protein is 47. 7 %, 45. 0 % respectively
Huangya14 )為材料分離克隆到一個細胞色素p450基因,命名為bccyp86mf5 , cdna全長1854bp ,含1575bp的完整開放閱讀框,編碼524個氨基酸,其編碼蛋白質的分子量為61 . 2kda 、等電點為8 . 96 ;堿性氨基酸、酸性氨基酸、疏水氨基酸和極性氨基酸分別占總氨基酸的13 . 74 、 11 . 64 、 36 . 45和22 . 70 ;二級結構預測包括n -糖基化位點、依賴于camp和cgmp的蛋白激酶磷酸化位點、蛋白激酶c磷酸化位點、酪蛋白激酶磷酸化位點、酪氨基酸激酶磷酸化位點、 n -豆蔻酰化位點和細胞色素p450的典型區域,半胱氨酸亞鐵血紅素配體信號區等, -螺旋和-折疊分別佔47 . 7 、 45 . 0 ;與bccyp86mf1基因的氨基酸序列同源性達到95 . 2 ,與擬南芥cyp86c4的達到85 . 9 。It ' s pi is 5. 2 as determined with ief. amino acid composition analysis showed that one subunit of 6 - phosphogluconate dehydrogenase has about 480 amino acids and there are plentiful of ala, asp, leu, ser, glu, thr, phe, lys in it
氨基酸組成分析表明:枯草芽孢桿菌6 -磷酸葡萄糖酸脫氫酶的亞基由約480個氨基酸殘基組成,富含丙氨酸、門冬氨酸、亮氨酸、絲氨酸、谷氨酸、蘇氨酸、苯丙氨酸、賴氨酸。Dna sequencing of the appa gene showed an open reading frame of 1299 bp. the deduced appa phytase composed of 432 amino acids ( predicted molecular mass, 47. 06 kd ) also contained the reserved active - site motif rhgxrxp, which is shared by other phytases and acid phosphatases
測序結果顯示appa基因閱讀框架為1299bp ,編碼432個氨基酸,編碼產物理論分子量為47 . 06kd ,同時它也具有其它植酸酶與酸性磷酸酶的活性保守基序rhgxrxp 。One solobp ecor i fragment containing phospholipase gene was isolated. further sequence analysis and subcloning revealed a 963bp phla. gene coding a 320aa phospholipase phl with deduced molecular weight of 33kd
通過測序及亞克隆分析,發現一個磷酯酶的基因phla ,長度為963bp ,預測編碼一個由320個氨基酸組成,分子量為33kd的磷酯酶phl 。Assessment of cholinesterase ( che ) inhibition is widely used as a specific biomarker for evaluating the exposure of non - target organisms to the pollutants, organophosphorus pesticide and carbamate
摘要應用水生生物膽堿脂酶作為生物標志物對水環境中有機磷和氨基甲酸鹽殺蟲劑的暴露和效應進行評價日益受到重視。Resistance would be developed when the activities of any of these three enzymes are increased. the three insecticide target - sites are acetylcholinesterase ( ache ) for organophosphate and carbamate insecticides, voltage - gated sodium channels : knockdown resistance ( kdr or kdr - type ) for ddt & pyrethroids and y - aminobutyric acid ( gaba ) receptors for cyclodienes
殺蟲劑的作用靶標主要有:有機磷和氨基甲酸酯殺蟲劑的作用靶標?乙酰膽堿酯酶; ddt和擬除蟲菊酯類殺蟲劑的作用靶標?神經軸突鈉離子通道;環戊二烯類和吡唑類等殺蟲劑的作用靶標? -氨基丁酸( gaba )受體-氯離子通道復合體。Method for simple determination of organophosphorus and carbamate pesticide residues in meat enzyme inhibition method
肉中有機磷及氨基甲酸酯農藥殘留量的簡易檢驗方法酶抑製法分享友人