洗過的沉澱 的英文怎麼說
中文拼音 [xǐguòdechéndiàn]
洗過的沉澱
英文
washed precipitate- 洗 : 洗動詞1 (用水等去掉物體上的臟東西) wash; bathe 2 [宗教] (洗禮) baptize 3 (洗雪) redress; ri...
- 過 : 過Ⅰ動詞[口語] (超越) go beyond the limit; undue; excessiveⅡ名詞(姓氏) a surname
- 的 : 4次方是 The fourth power of 2 is direction
- 沉 : Ⅰ動詞1 (沉沒; 墜落) sink 2 (沉下 多指抽象事物) keep down; lower 3 [方言] (停止) rest Ⅱ形容...
- 沉澱 : 1 (沉澱過程中析出的物質) sediment; precipitate; sedimentary accretion; precipitation; (doposit...
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Get the inclusion bodies 2 ) western blot analysis of fusion protein expression ( 1 ) electrophoresis ( 2 ) transfer proteins from gel to membrane ( 3 ) blocking ( 4 ) incubation with primary antibody ( 5 ) enzyme conjugate incubation ( 6 ) substrate incubation. 3 ) gst detection module with cdnb enzymatic assay 3 purification of gst fusion proteins 1 the denaturalization of inclusion bodies. 2 purification using glutathione sepharose 4b column wash matrix with 1 pbs, prepare a 50 % slurry for batch purification method, pack column with matrix slurry
三、 gst一hbrp重組蛋白的純化1 .超聲破碎細胞,離心,上清和沉澱進行sds一page電泳分析2 .樣品處理提取包涵體,變性后,加人用pbs平衡過的以utal腸onesepb抓脫4b ,室溫下孵育3 .以utadtionesepharose4b柱純化以utad雲onesepharose4b柱的準備根據毛山lel ,決定純化所需的gll衛ta1如oneseph娜se4b的柱床體積,用預冷的1xpbs清洗cldtathionese戶, se4b ,得到50 %的基質In environment water treatment aspect, there are professional technology staff who are working on environment protect and dedicate to transplant foreign advanced technology, meanwhile they develop acidity / alkalescence waste water treatment technologies and equipment, soft water desalination technologies and equipment, high effectual lime preparation and putting set, high effectual integration putting set, at the same time, develop gravity sand filters, bevel precipitate set, many series of filter set, super filter set, prevent infiltration set, cooling tower, large - sized frothing water tank, cleaning device etc. all these technologies and equipments are domestic leading level
環保水處理方面,有專業從事環保方面的技術專業人員,在從事多方面環境污染治理工作的基礎上,致力於消化移植國外先進技術,研製開發出酸性/堿性廢水處理工藝和成套設備、軟水脫鹽水工藝和成套設備、高效石灰乳制備及投加裝置、高效一體化加藥裝置,同時開發出重力砂濾器、斜板沉澱器、多種過濾器、超濾裝置、反滲透裝置、冷卻塔、大型鼓泡水箱、清洗裝置等單體設備,這些工藝技術與設備均居於國內領先水平。The ascites fluid antibody purified by 33 % ammonium sulfate is further purified by chromatography. the p24 affinity chromato - graphic column done to purify the bispecific antibody is in the brcn activated sepharose 4b. after washing the unspecific proteins, change the washing buffer and the anti - p24 / anti - human rbc type a bispecific antibody and the anti - p24 monospecific antibody are gained respectively
方法是先用硫酸綏鹽析,再過親和層析柱,即33飽和硫酸按沉澱后,透祈過夜,上p24抗原做配體的濱化氰活化的sepharose4b柱,流洗掉雜蛋白和抗人紅細胞單特異性抗體后,再洗脫並收集雙特異性抗體。After washing with reagent ( 50mmol / l ph8. 0 tris - hcl, 100mmol / l nacl, 0. 5mmol / l edta, 2mol / l carbamide, 0. 2 % triton x - 100, 0. 2 % doc ), ultrasound crushing ( 200 w, 150 times, 3 seconds per time, spacing 3 seconds ) and freezing - melting methods, we got hng fusion protein and m - insulin fusion protein with purity of above 80 %. 4. radioimmunoassay result shows that the radioimmune activity of of m - insulin fusion protein is 0. 5 unit per litre bacterial liquid
3 .表達產物初步純化仁gly 」一flumanin融合蛋白和人胰島素突變體融合蛋白包涵體的洗滌方案為: lj ]體沉澱溶於含zmol , / l尿索洗滌液,超聲( 200w 、巧o次、 3秒/次、間隔3秒) , 4 、 i000orpm離心15min后沉澱用洗滌液充分重懸,一2 ( )凍存過夜,次日融化后4 、 10000rpm離心15min ,取上清,即得到初步純化的融合蛋白,可去除土要的雜蛋白,融合蛋白溶解于上清液中,純度達到80 %以_ 1 : 。Corresponding soluble salts have been chosen as raw materials and ammonia as precipitator. the optimum ph value is 8. 5 - 10 and higher concentration is helpful for crystal growth. the perovskite phase plzst can be synthesized at 750 by solid state reaction
合適的工藝條件為:選擇以各離子的可溶性鹽為原料,以氨水為沉澱劑,通過共沉澱得到白色無定型前驅體,最佳的ph值范圍為8 . 5 10 ,溶液的濃度越高越有利於固相反應合成中晶體的形成,適當的沉澱后處理過程如水洗、干磨能促進plzst晶體的生成。分享友人