活性誘發因子 的英文怎麼說

中文拼音 [huóxìngyòufāyīnzi]
活性誘發因子 英文
competence inducing factor
  • 活 : Ⅰ動詞1 （生存; 有生命） live 2 [書面語]（救活） save （the life of a person）:活人無算 （of a goo...
  • 性 : Ⅰ名詞1 （性格） nature; character; disposition 2 （性能; 性質） property; quality 3 （性別） sex ...
  • 誘 : 動詞1. （誘導） guide; lead; induce 2. （使用手段引人隨從自己的意願） lure; seduce; entice
  • 發 : 名詞（頭發） hair
  • 因 : Ⅰ動詞[書面語] （沿襲） follow; carry on Ⅱ介詞1 [書面語] （憑借; 根據） on the basis of; in accord...
  • 子 : 子Ⅰ名詞1 （兒子） son 2 （人的通稱） person 3 （古代特指有學問的男人） ancient title of respect f...
  • 活性 : [化學] activity; active; activated活性肥料 active fertilizer; 活性酵母 active dry yeast; 活性粘土...
  • 誘發 : bring out; induce; cause to happen
  1. Curcumin has been shown to induce a wide variety of tumor cells by several mechanisms, mainly including modulation of the expression of oncogenes and cancer suppressor genes, down - regulation the activation of transcription factors, via many signal transduction pathways, and the modulation of cell cycle. provides an overview of domestic and international apoptosis mechanisms of malign tumor cells induced by curcumin in order to better explore and open up new avenues of cancer treatment

    姜黃素能夠誘導多種腫瘤細胞系凋亡，其機制主要是調控癌基因和抑癌基因，下調多個轉錄因子活性，通過多種信號轉導途徑，誘發細胞周期停滯而誘導細胞凋亡.對近年來姜黃素誘導細胞凋亡的機制進行綜述，以便更好地探求和開辟治療惡性腫瘤的新途徑。
  2. No, a first gas information molecule discovered in human being, is a typical endothelial - derived relaxant and mediates endothelium - dependent relaxation of blood vessels. in the pathogesis of endotoxin shock vec is one of the major target cells of lps and lps - induced proinflammatory cytokine such as tumor necrosis factor and interlukin 1 and activated. in vec inducible nitric oxide synthase ( inos ) is induced and lead to an increase in production of no, the while endothelial nitric oxide synthase ( enos ) is inhibited and elicit decrease in no formation, both of which are demonstrated to induce the

    在內毒素休克過程中vec是lps及其誘導機體產生的多種促炎細胞因子如tnf 、 il - 1作用的主要靶細胞， vec誘導型一氧化氮合酶（ induciblenitricoxidesynthase ， inos ）激活、 no大量誘生而內皮型一氧化氮合酶（ endothelialnitricoxidesynthase ， enos ）活性被抑制、 no生成障礙，是血管反應性異常變化、血管調節機制紊亂的重要發病環節。
  3. The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins

    將帶有hwtx -基因的ppic9k經blgii線性化后，轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌，進一步用遺傳毒素g418篩選多拷貝的轉化菌株，命名為gh1 ；將gh1甲醇酵母菌用0 . 5的甲醇誘導表達，發酵上清經90飽和度的（ nh _ 4 ） _ 2so _ 4沉澱， yw - 3 （ mwc03000 ）的超濾膜超濾，再經cm陽離子交換， c _ （ 18 ）反相hplc純化得到分子量為4kd左右的組分，其中4289 . 05的組分經質譜鑒定，氨基酸組成分析和序列測定為正確的表達產物，生物學活性表明其活性為天然毒素活性70 % ，表達量為80mg / l 。
  4. Elicitors molecules that initiate cell signaling pathways leading to the activation of plant defense genes and the production of phytoalexins and other defensive chemicals in response to infection by a pathogen

    誘導子：可以引發細胞信號通路的分子，使植物防禦基因、植物抗毒素及其他防禦性化學物質製品被活化以防病原體的感染。
  5. Results steroid receptor coactivators are essential for several kinds of steroid hormones binding to steroid receptors, so they are important accessory factors that induce the initiation, development and recurrence of breast cancer, and predictive factors that estimate the prognosis of breast cancer

    結果類固醇受體輔活化因子是多種類固醇激素與類固醇受體結合併發揮生物學活性的必需因子，是誘導乳腺癌發生、發展和復發的重要輔助因子，同時也是判斷乳腺癌預后的重要預示因子。
  6. To prove that the cloned dna fragment can express tryptopanase, a new plasmid pet28c - tnaa, in which the cloned dna fragment was located downstream of t7 promoter on pet28c was constructed and transformed into host bl21 ( de3 ), a bl21 lysogen of bacteriophage de3 in which the only promoter known to direct transcription of the t7 rna polymerase gene is the lacuvs promoter, which is inducible by iptg

    用iptg誘導表達t7rna聚合酶，以表達質粒上的目的基因。在葡萄糖存在的條件下，用常規方法發酵和誘導（ 37 1mmiptg ） ，發現表達的蛋白質條帶的分子量與理論上計算的分子量一致。但是發酵液中檢測不到吲哚，表明雖然表達了目標蛋白，但表達的蛋白質沒有酶活性。
  7. Porcine il - 12 ( pil - 12 ), also termed as natural killer cell stimulatory factor ( nksf ) and cytotoxic lymphocyte maturation factor ( clmf ), can induce some cytokines, particularly ifn -, from t and nk cells, as well as enhance the cytotoxic activity of these cells. its most critical role may be to initiate the development of cell - mediated immunity by promoting the differentiation of th1 cells from naive t cells

    白細胞介素- 12 （ il - 12 ）又名nk細胞刺激因子（ nksf ）或細胞毒淋巴細胞成熟因子（ clmf ） ，能誘導t細胞和nk細胞產生ifn - ，並增強其細胞毒活性，它的關鍵作用是能誘導th0細胞分化為th1細胞以激發細胞免疫進程。
  8. Western blot analysis showed that rhpk - 5 ( recombinant human plasminogen kringle 5, rhpk - 5 ) protein was recognized by mab same as native hpk - 5. the result suggested that we obtained correct gene sequence of hpk - 5 and got the purified rhpk - 5. section ii : construction of pbv220 / hpk - 5 vector for obtaining high - level hpk - 5 expression system, the hpk - 5 gene was recombined with plasmid pbv220 to construct the vector of pbv / hpk - 5

    Coli ）作為宿主，經sds - page分析，篩選表達量最高的菌株作為發酵用工程菌株；用western - blot方法鑒定hpk - 5因子的免疫學活性；用搖瓶發酵的方法，研究發酵培養基的體積（溶解氧） 、組成成份及誘導起始時間和誘導持續時間對目的蛋白表達量的影響，優化hpk - 5基因工程菌的表達條件。
  9. Both spla 2 and ceramidehave been shown to inhibit bradykinin -, and pma - induced phospholipase d activation. these results indicated the existence of a subtle intertwined regulatory interaction between intracellular phosphoglycerol lipids and sphingolipids signaling pathways. in addition, we also found that spla can 2 down regulate theepidermal growth factor ( egf ) receptor activation

    此外，我們還發現分泌型磷脂酶a能下調表皮生長因子受體的活化，它能明顯的抑製表皮生長因子誘導2的受體自身磷酸化，並抑製表皮生長因子誘導的磷脂酶d活性和磷脂酶c - g的1酪氨酸磷酸化。
  10. After purification using glutathione sepharose 4b affinity chromatography and digestion with thrombin, the recombinant ntfs were found to be biologically active in the pc 12 cells neurite outgrowth assay. the assays demonstrated that the purified ntfs proteins exhibited normal activity, which is the first step in developing a comprehensive gene therapy for nerve diseases of the giant panda and the crested ibis

    最後，對重組表達蛋白經glutathlonesepharose4bmicrospincolumn親和純化后，進行大鼠腎上腺嗜鉻瘤細胞（ p2 ）神經營養因子的活性鑒定，發現其能夠誘導神經細胞分化產生突觸，即具有預期的生物學活性，表明所獲表達蛋白為大熊貓nt
  11. The mutant pel - d92l was expressed in pichia pastoris gs115, sds - page detection showed that the expression product pel - d92l - gs is different from pel - gs, and its " yield decreased dramatically, the themostability of pel - d92l - gs is also different from the pel - gs, but their optimum temperatures are same. 3. directed evolution of pel through random mutagenesis mutagenesis pcr carried out in error - prone conditions was used on the vector psk - pel, using the oligos " beginning " and " end ", homologous to the 5 ' and to the 3 " ends of the gene of pel respectively

    三、 pel基因的隨機誘變用易錯pcr方法對pel基因進行隨機誘變， pcr產物與ppic3 . 5k連接，轉化大腸桿菌，獲得的混合質粒電轉化畢赤酵母gs115 ， omm平板篩選適于低溫或對熱穩定的重組子，篩選獲得一株最活反應溫度、熱穩定性、發酵酶活均有提高的突變體pel - ep5 - gs ，其最活反應溫度為45 ，比野生型高出5 ； 40處理30min殘留活性為56 ，大大高於野生型的6 ；初始ph7 . 3528條件下培養72h ，發酵上清酶活為325u / ml 。
分享友人
分享到 Line

分享到臉書