活細胞培養 的英文怎麼說
中文拼音 [huóxìbāopéiyǎng]
活細胞培養
英文
biocytoculture- 活 : Ⅰ動詞1 (生存; 有生命) live 2 [書面語](救活) save (the life of a person):活人無算 (of a goo...
- 細 : 形容詞1 (條狀物橫剖面小) thin; slender 2 (顆粒小) in small particles; fine 3 (音量小) thin ...
- 胞 : Ⅰ名詞1 (胞衣) afterbirth2 (同一個國家或民族的人) fellow countryman; compatriot Ⅱ形容詞(同胞...
- 培 : 動詞1. (在根基部分堆上土) bank up with earth; earth up 2. (有目的地使成長、壯大) cultivate; foster; train
- 養 : Ⅰ動詞1 (供養) support; provide for 2 (飼養; 培植) raise; keep; grow 3 (生育) give birth to ...
- 細胞 : cell; sytes; bioplast; cella; [口語] gene; [生物學] cellule; cellule cellulli cellulo ; cello ; k...
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We hope that our study will provide us with more comprehensive knowledge of the mechanisms of immune regulation and the roles that dc and complement play in innate and acquired immunity, as well as to lay a foundation for further exploration of the roles dc play in antigen - specific immune responses and immune tolerance from a new perspective. part i expression of complement receptors and complement - associated molecules on dendritic cells derived from distinct - origin at different stages of development two subsets of dendritic cells were generated from precursor cells isolated by means of magnetic cell separation system
曰補體受體及其相關分子在modc上的表達不同分化階段的單核細胞衍生性dc ( monocytes一deriveddc , modc )的誘導:將新鮮分離的單核細胞mo ,在含有gm一csf和工l一4培養體系中誘導5一7d ,即分化為未成熟modc ;對培養至sd的未成熟modc ,用tnfa刺激zd ,即分化為成熟modc ;此時再用lps刺激24h ,即為活化的modc 。The primary results showed : using m199 as diluents containing 20 % bovine serum, it is better to freeze the cells slowly freezing at fist then increase freezing speed ( for example, from 0 to - 6 freezing speed is about - 0. 05 a minute, from - 6 to - 40, freezing speed is about - 0. 5 a minute ), studies on effect of various concentration of dmso demonstrate that about 12. 5 % dmso gave the highest post - thaw percentage of viable cells. the concentration of bovine serum had no different effect on the percentage of the viable embryo cells of misgurnus auguillicaudatus. the embryo cells derived 6 from the later stage of blastula offish is more resistant to the cryogen than the cells of early stage of blastula. the cells preserved in liquid nitrogen at - 196 were thawed and cultivated, a few cells were found adhere to the surface of culture vessel when the percentage of viable cell was more than 30 %. the cells in only two culture vessels were found to proliferated and gave rise to many small morphologically undifferentiated cells
研究初步表明:以細胞培養液m199 (含2既的小牛血清,常規量雙抗)為凍存稀釋液對泥鰍胚胎細胞冷凍保存宜採取先慢后快的方式(例如,從0一一6 ,凍存速度為一0 . 05 / min ,再以一0 . 5 / min的速度從一6一一40 ) ; dmso的保護效應濃度為12 . 506左右;小牛血清的濃度對泥鰍胚胎細胞的成活率影響不明顯;囊胚晚期細胞抗凍性比中早期強;通過對不同批次的凍存細胞解凍培養,解凍后成活率為30 %以上細胞培養數天後均有少數細胞貼壁,但只發現兩瓶培養細胞有明顯增殖現象產生許多未分化的小細胞。Using m199 containing 20 % calf bovine serum and 11 % dmso as the diluent and by the methods using in cryopreservation of embryo cells of misgurnus auguillicaudatus, two groups of cells derived from blastula of grass carp were preserved in liquid nitrogen at - 196. after 6 days cryopreservation, one group of cells were thrawed and the percentage of viable cell was about 72 % ; the other, cryopreserved for 13 days, was 52
以細胞培養液m199 (含20 %的小牛血清)為稀釋液, dmso的濃度為11 % ;與泥鰍胚胎細胞冷凍保存方法一樣,採取先慢后快的方式,冷凍保存兩組草魚囊胚晚期細胞於一1 %的液氮中。第一組冷凍保存6天後解凍,成活率為72 % ,第二組冷凍保存13天後解凍,成活率為520 / 0 。Conclusion : the system not only could pick upon the op timum strain on fixed culture medium or the optimum substrate to special strain fleetly but also could be used to evaluate the microbe growth character apace, monitor z ymogen upgrowth in fermentor and determine total number of bacteria in fresh cre amery rapidly
結論:研製的生物傳感器系統可用於菌種和培養基組分的快速篩選、微生物活細胞生長特性的快速評估,還可用於發酵罐中發酵菌的生長監測及新鮮牛奶中雜菌總數的快速測定。To study the suitable method for cattle oviduct simple epithelium cells culture, the epithelium cells were isolated by cutting and 0. 25 % trypsinization, the exponential phase of growth cells vigor and growth velocity was determined by mtt method, the viable count was detected by the rejection experiment of trypanblau
摘要為探討適用於黃牛輸卵管單層上皮細胞的培養方法,採用機械剪取及0 . 25 %胰酶消化的方法分離獲得上皮細胞,取對數生長期細胞進行mtt比色實驗檢測細胞活力和生長速度;臺盼藍排斥試驗檢測活細胞數。Abstract : adopting the serum - free and animal - source - free medium domestication express cell efficiently, setting up to express system efficiently, suspending culture cell, can raise the cell density in the scale turn the production, strengthen the cell vitality, control cell to propagate level, extension cell culture period, increase the target protein of yield, raise product quality, simplification of produces technics, reduce production cost, then raising the efficiency that the scale turns culture
提要:採用無血清無動物組分培養基馴化高效表達細胞,構建高效表達系統,懸浮培養細胞,可以在規模化生產中,提高細胞密度,增強細胞活力,控制細胞增殖水平,延長細胞培養周期,增加目標蛋白的產量,提高產品質量,簡化生產工藝,降低生產成本,進而提高規模化培養的效能。After adding culture mediem of stably transfected jurkat cells to hepatocarcinoma cells, the binding specificity of the scfvs with hbsag was further confirmed by observation by fluorescence microscope, indirect immunofluorescence and flow cytometer analysis. prokaryotic expression plasmids ptat - ha - scfvs were successfully constructed
建系細胞培養上清與肝癌細胞作用后,經熒光顯微鏡觀察、間接免疫熒光及流式細胞儀檢測進一步確定表達的scfv融合蛋白具有與hbsag特異性結合的活性。Methods for qualitative and quantitative characterization of primmorph culture were developed, which included a modified mtt assay for measurement of cell viability and cell growth, a 5 - brdu - incorporation assay for detection of cell proliferation, propagation and metabolism of in vitro primmorph
系統考察並優化了mtt法應用於海綿細胞培養過程細胞活性和細胞生物量定量的條件,結合5 - brdu摻入法建立了細胞團培養過程中細胞增殖能力和細胞活性的評價方法。In terms of human cell cloning, he gained a great deal of experience
在人體活細胞(克隆)培養增殖技術方面積累了豐富的經驗。The methods of evans and martin were changed slightly and used to isolate the mouse es cell in my experiment. in brief, the intact blastocysts were plated on sto feeder layer treated with mitomycin, and were cultured in the media supplymented with brl condition medium
聯合evans和martin的方法,稍加改良來分離小鼠胚胎幹細胞,把昆明白小鼠完整的囊胚直接種植在經絲裂黴素滅活的sto飼養層細胞上,在含有brl條件培養基的es細胞培養液中培養。Sections were stained by he and were observed under light microscope. ( 4 ) observation on cell - matrix complex with confocal microscope. cell - matrix complex was stained by fluorochrome cfda - am ( loug / ml, looul ) after 7 days incubation, the sample was scanned by confocal microscope to observe cell - growth in the matrix
細胞一多孔膜復合物的激光共聚焦顯微鏡觀察:取培養7天的細胞一多孔膜復合物,以10ug inl的熒光染料cfad am100ul染色,檄光共聚焦顯微鏡檢測激光激發的綠色熒光,掃描成像觀察活細胞生長倩況。Many cell culture and animal experiments have been conducted to investigate the efficacy of these botanical compounds, however, indicating the potential for many beneficial effects such as increased collagen expression, improved antioxidant activity, accelerated healing and enhanced hydration
雖然進行了許多考察這些植物化合物的有效性的細胞培養試驗和動物試驗,但僅表明他們具有一些潛在的治療作用,諸如促進膠原表達,提高抗氧化劑的活性,促進愈合以及增強水和作用。The university is seeking government s support for the establishment of the brain bank so that fresh brain tissues can be stored and other sophisticated tests, e. g. brain cell culture, can be carried out. the materials in the bank can be used by scientists and doctors working anywhere in hong kong and will surely benefit the society in the long term
要對中國人癡呆病作更深入的研究,需要一個更全面的大腦組織庫,並可以貯藏新鮮組織、活細胞或神經細胞培養的庫,香港中文大學計劃向大學教育資助委員會和政府申請撥款,支持研究這個人口不斷老化的社會一定要面對的問題。Cell morphology was similar no matter what method was used. percentage of live cells was significantly higher in erythrocyte splitting method that was ( 91 4 ) % than in centrifugation method that was ( 83 5 ) %. proliferative ability of mscs seperated by erythrocyte splitting method at 2 to 6 generation was much higher than by another method. the maximal cell number was 4. 67 vs 4. 10 times that of the initial cell number at cell inoculation
紅細胞裂解法較密度梯度離心法細胞貼壁較慢, 36小時才可見零星細胞貼壁,兩種方法細胞形態相似。活細胞百分比,紅細胞裂解法為( gi士4 ) ,密度梯度離心治( 83士5 ) ,有明顯差異。傳代培養: 2 6代范圍內,紅細胞裂解法較梯度離心法細胞增殖更旺盛(分別是按種時細胞數的4Effect of selenium and iodine levels on calcium overload and actin expression in rat liver
研究提高細胞培養溫度對仔豬肝細胞一磷酸腺苷激活蛋白激酶The biomacromolecular layers on the plla membrane surfaces had good stability and good cytocompatibiliry. cells spread very well on the gelatin or collagen coated membranes and had obviously improved adhesion rate, proliferation rate and mtt activity
軟骨細胞培養結果表明明膠塗層或膠原塗層可明顯提高細胞在plla膜表面的粘附率、增殖率、細胞活性和鋪展性能。The products cover the fields of the study of molecule biology, expression of protein, from cell culture to layer purification, from antigen antibody, the whole set reagent kit to the microorganism culture and the research of theactivity of the biology
其產品覆蓋了生命領域從分子生物學研究、蛋白表達、細胞培養到層析純化,從抗原抗體、成套試劑盒到微生物培養及其生物活性研究等相關試劑幾近所有過程。The other 15 peptides were classified into 2 groups, each group sharing a different consensus sequence that might bind a different site of the target. one peptide of each group was chemically synthesized and added to the rdrp reaction system. we found that one peptide refrained the activity of rdrp
自2組結合膚中各選擇1個環膚分子用化學方法合成,將合成膚加入rdrp反應體系后,其中1個環膚分子對rdrp活性表現明顯的拮抗作用,加入細胞培養中可對den感染細胞產生明顯的保護作用。In order to improve titanium alloy ' s biocompatibility, the titanium dioxide coating was fabricated on ti matrix. besides, the surface of titanium alloys was directly disposed by alkali to make it possess bioactivity. biocompatibility and bioactivity of the material after surface modification were evaluated through the simulated body fluid ( sbf ) cultivation and cell cultivation experiment
為了提高材料的生物相容性,本研究對自製的鈦基體進行了二氧化鈦塗層的制備和表面堿液生物活化處理的研究,並採用模擬體液培養實驗和細胞培養實驗評價了表面改性后材料的生物相容性和生物活性,對基體以及表面改性后材料的組織形貌、相組成和生物相容性進行了觀察、分析與評估。Great dictionary of world scientific experts and talents as successful chinese by world invention and development center in america
在人體活細胞克隆培養增殖技術方面積累了豐富的經驗。分享友人