流式細胞儀 的英文怎麼說
中文拼音 [liúshìxìbāoyí]
流式細胞儀
英文
flow cytometer- 流 : Ⅰ動1 (液體移動; 流動) flow 2 (移動不定) drift; move; wander 3 (流傳; 傳播) spread 4 (向壞...
- 式 : 名詞1 (樣式) type; style 2 (格式) pattern; form 3 (儀式; 典禮) ceremony; ritual 4 (自然科...
- 細 : 形容詞1 (條狀物橫剖面小) thin; slender 2 (顆粒小) in small particles; fine 3 (音量小) thin ...
- 胞 : Ⅰ名詞1 (胞衣) afterbirth2 (同一個國家或民族的人) fellow countryman; compatriot Ⅱ形容詞(同胞...
- 儀 : 名詞1 (人的外表) appearance; bearing 2 (禮節; 儀式) ceremony; rite 3 (禮物)present; gift 4 ...
- 細胞 : cell; sytes; bioplast; cella; [口語] gene; [生物學] cellule; cellule cellulli cellulo ; cello ; k...
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Facs results showed that umbilical cord blood mscs did not express antigens cd34, cdlla and cdllb, and expressed cd29 and cd71, which was identical to that of human bone marrow - derived mscs
用流式細胞儀檢測msc的表面標志結果顯示,臍帶血msc不表達cd34 、 cd11a和cd11b ,強表達cd29 ,弱表達cd71 ,與骨髓msc表面抗原特性一致。Thus, in order to investigate the developmental pathways not only involved in the regulation of growth and patterning, but also in the determination of cell lineages and differentiation, we utilized the fluorescent immunohistochemical methods, flow cytometry analysis sorting ( facs ) and molecular methods to investigate the developmental law of mammary gland at the different developmental stages, distribution of the stem cells in mammary gland, the methods of isolation, culture and evaluation for the stem cells, the multipotent abilities in vivo and in vitro, and the efficient cultural system for stem cells enriched in vitro. the results showed below : 1
我們以小鼠為模型,運用組織化學、免疫熒光組織(細胞)化學、流式細胞儀分選方法( facs )以及分子生物學手段,研究了小鼠乳腺的發育規律:小鼠乳腺組織中類乳腺幹細胞:小鼠乳腺細胞的分離、培養以及類乳腺幹細胞的鑒定;小鼠類乳腺幹細胞分化的潛能;小鼠乳腺類腺體體外短期培養富集類乳腺幹細胞體系的優化等。研究結果表明: 1Study of the relationship between cell size, chlorophyll content of aegiceras corniculatum mesophyll and seawater salinity by fcm
用流式細胞儀探討桐花樹葉肉細胞大小和葉綠素含量與海水鹽度的關系Mdr1 express product p - glycoprotein was detected by immunocytochemical method and flowcytometry. the cytotoxicity and multidrug resistance reversion effect of tea polyphenol was examined by mtt assay in mcf - 7 and mcf - 7 adr carcinoma cell lines, and compared with pgp inhibitor quinidine. the pgp expression of mcf - 7 adr was strongly positive, the positive rate was 15 % ; the pgp expression of mcf - 7 was negative, the positive rate was 1. 8 %. ic50 of tea polyphenol to mcf - 7 and mcf - 7 adr is 115. 2g ml and 207. 6g ml respectively. ic50 of quinidine to mcf - 7 and mcf - 7 adr is 129. 8mol l 42. 1g ml and 94. 1mol l 30. 5g ml respectively. tea polyphenol and quinidine changed little toxicity of adriamycin to mcf - 7, but tea polyphenol and quinidine improved the sensitivity of mcf - 7adr to adriamycin significantly. immunocytochemistry and flow cytometry can detect p - glycoprotein expression level qualitatively and quantitatively. tea polyphenol is not only an anti - tumor agent, but also a multidrug resistant modulator similar as quinidine. tea polyphenol is advantageous for its little toxicity in tumor treatment
用免疫組化法和流式細胞儀對腫瘤細胞系mcf - 7和mcf - 7 adr的p -糖蛋白表達水平進行定性定量研究。用噻唑藍比色法mtt研究茶多酚的細胞毒性及其對耐藥性的逆轉作用,並與pgp抑制劑奎尼定進行了比較。免疫組化法檢測p -糖蛋白表達水平, mcf - 7 adr呈強陽性,而mcf - 7呈陰性流式細胞儀定量檢測結果mcf - 7 adr細胞系細胞陽性率為15 % , mcf - 7細胞系細胞陽性率為1 . 8 % 。In our report, we selected trehalose to substitute sucrose in the cryoprotectant. the process of vitrification is under the same preferable condition. the survival - rate of the recovered cells was tested by fda - pi and cd34 + cell - count
凍融后的細胞以fda - pi雙熒光染色、流式細胞儀cd34 +計數等檢測手段,與同樣條件的60蔗糖保護劑處理的細胞相比較。The principle of flow cytometer and its clinical application
流式細胞儀工作原理及臨床應用3. observe the binding of oligochitosan labeled with 2 - amac with macrophages under confocal laser microscope and analysis the fluorescence intensity by flow cytometer using the cell quest software
在激光掃描共聚焦顯微鏡下觀察2 -氨基吖啶酮標記殼寡糖與巨噬細胞的結合,用流式細胞儀分析熒光強度。After adding culture mediem of stably transfected jurkat cells to hepatocarcinoma cells, the binding specificity of the scfvs with hbsag was further confirmed by observation by fluorescence microscope, indirect immunofluorescence and flow cytometer analysis. prokaryotic expression plasmids ptat - ha - scfvs were successfully constructed
建系細胞培養上清與肝癌細胞作用后,經熒光顯微鏡觀察、間接免疫熒光及流式細胞儀檢測進一步確定表達的scfv融合蛋白具有與hbsag特異性結合的活性。Cell compatibility of films is researched firstly, which will make a significant contribution to the using fha films in practice from development. cell cycle, measured by flow cytometer and mtt method, and cell growth curve are used to analyze the impact of material and the immersed medium to the multiplication of osteoblast - cell
通過mtt法,流式細胞儀測定細胞周期,以及細胞生長曲線的測定,分析研究了fha薄膜材料對成骨細胞增殖生長的影響以及材料的浸提液對細胞增殖的影響,通過細胞相對增殖活性的測定對fha薄膜進行毒性評級。Et and nos immunochemical staining and the analyzing the apoptosis in acute myocardial ischemia may be a sensitive method to diagnose the sudden death caused by ami in forensic medicine
故此,通過流式細胞儀檢測心肌的凋亡指數並結合超敏sp法對心肌進行et 、 inos和enos聯合免疫組化染色,可以輔助診斷ami所致的碎死。1, due to the limitation of existing assay for evaluation of cell proliferation and cytotoxicity, in this study we developed a novel method
基於目前測定免疫細胞增殖殺傷的方法的局限性,本研究首先建立一種用流式細胞儀測定該指標的新方法。Methods : 1 ) 12h after irradiation, the cell cycle of nih3t3 cells was determined by flow of cytometry and the ratio of alternations in p16 gene exon - 2 was evaluated through pcr - sscp. 2 ) the content of mda, the activities of the sod and gsh - px in the supernatant of nih3t3 cells and the cells were measured by detecting kits immediately after irradiation. 3 ) the level of matrix metalloproteinase - 2 ( mmp - 2 ) in hela cells was detected by western - blotting and dot - blotting 2h after irradiation
具體方法為: ( 1 )照射后12h ,收集nih3t3細胞,用流式細胞儀檢測各組細胞的細胞周期, pcr - sscp檢測抑癌基因p16的變化; ( 2 ) nih3t3細胞照射后立即收集細胞和細胞上清,用試劑盒測量mda含量和sod 、 gsh - px的活性並觀察其變化; ( 3 ) western免疫印跡和點雜交法檢測照射2h后的各組hela細胞中基質金屬蛋白酶- 2 ( mmp - 2 )的表達變化。Study on action of flow cytometry on apoptotic cell
流式細胞儀對凋亡細胞作用的研究( 4 ) cell cycle distribution by flow cytometry
( 4 )流式細胞儀檢測細胞周期分佈。Pictured here is the flow cytometry pattern of a breast carcinoma
圖示:乳腺癌流式細胞儀模式圖。Flow cytometry analysis of reticulated platelets : primal evaluation of clinical blood disorders
流式細胞儀計數網織血小板及初步臨床應用Establishment and clinical application of quantitative method for detecting phagocytosis rate of macrophages by flow cytometry
流式細胞儀測定巨噬細胞吞噬率方法的建立及其應用Apoptotic peak. agarose gel electrophoresis showed that 185 - base pair ladder characteristic of the dna degradation that occurs in apoptotic cells induced by a
電泳出現階梯狀條帶流式細胞儀檢測出現典型的凋亡峰,提示It was finded by using flow cytometry that the apoptosis rate of porcine ear skin fibroblasts cultured under a variety of cell cycle - arresting treatments was quite low
通過流式細胞儀檢測,發現不同處理的豬耳皮膚成纖維細胞的凋亡率均較低。In this work, effects of sfamnpv on mitochondrial and er in sl - 1 cells were studied. the main results were as follows : effect of sfamnpv ( moi = 6 ) on the function of mitochondrial in sl - 1 cells was investigated by mtt assay and membrane potential was assayed by both flow cytometry and confocal laser scanning microscope
完成的工作包括以下幾個方面: ( 1 )細胞內線粒體的變化通過mtt法研究病毒感染對細胞線粒體功能的影響,同時用流式細胞儀和共聚焦激光顯微鏡研究線粒體膜電位( m )的變化, western檢測2種凋亡調控蛋白cytoc和bcl - 2蛋白的變化。分享友人