溶菌素生成 的英文怎麼說
中文拼音 [róngjūnsùshēngchéng]
溶菌素生成
英文
lysinogenesis- 溶 : 動詞(溶化; 溶解) dissolve
- 菌 : 菌名詞1. (蕈) mushroom2. (姓氏) a surname
- 素 : Ⅰ形容詞1 (本色; 白色) white 2 (顏色單純) plain; simple; quiet 3 (本來的; 原有的) native Ⅱ名...
- 生 : Ⅰ動詞1 (生育; 生殖) give birth to; bear 2 (出生) be born 3 (生長) grow 4 (生存; 活) live;...
- 成 : Ⅰ動詞1 (完成; 成功) accomplish; succeed 2 (成為; 變為) become; turn into 3 (成全) help comp...
- 生成 : create; generate; produce生成演算法 generating algorithm; 生成文法 generating grammar; 生成物 pro...
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First, after investigation of two original strains " biological characteristics, we studied the main influence factors on protoplasts formation and regeneration in s. mycarofaciens and s. erythreus, and determined the best protoplasts formation and regeneration conditions of two original strains. the former shake - cultured in s " medium at 28 ?, 220r. min ~ ( - 1 ) for 24h, lysised by 3mg / ml lysozyme, keeping warm at 32 ? for 50 ~ 60min, regenerated on r _ ( 5 " ) medium, 28 ? for 5 ~ 6d. the latter used two - step culture, then used img / ml lysozyme keeping warm at 37 ? for ih ; the protoplasts were plated on r5 " regeneration medium at 28 ? for 5d
首先在對兩親株的生物學特性進行了鑒定后,考察了影響兩親株原生質體形成和再生的主要因素,確定了生米卡鏈黴菌和紅黴素鏈黴菌原生質體形成及再生的最佳條件:前者用s培養基,在28 、 220r . min ~ ( - 1 )培養24h后,用3mg ml的溶菌酶在32恆溫酶解50 60min ,得到的原生質體在乾燥的r5培養基上28倒置培養5 6天,可得到再生率在20左右的再生菌落;後者採用二級菌絲培養,用1mg ml的溶菌酶在37恆溫酶解1h左右,得到的原生質體也在乾燥的r5培養基上28倒置培養5天,即可得到再生率在20左右的再生菌。
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