熒光染色法 的英文怎麼說

中文拼音 [yíngguāngrǎnshǎifǎ]
熒光染色法 英文
fluorescent staining method
  • 熒 : 形容詞[書面語]1. （光亮微弱的樣子） glimmering 2. （眼光迷亂; 疑惑） dazzled; perplexed
  • 光 : Ⅰ名詞1 （照耀在物體上、使人能看見物體的一種物質） light; ray 2 （景物） scenery 3 （光彩; 榮譽） ...
  • 染 : Ⅰ動詞1 （用染料著色）dye 2 （感染） catch [contract] （a disease） 3 （沾染） acquire （a bad hab...
  • 色 : 色名詞[口語] （顏色） colour
  • 法 : Ⅰ名詞1 （由國家制定或認可的行為規則的總稱） law 2 （方法; 方式） way; method; mode; means 3 （標...
  • 染色 : dye; dyeing; colouration; tintage; tinging; dyschroia; colouring; colour; [半] decoration染色不足...
  1. First, to construct a recombinant plasmid pegfp - c - fos with c - fos promoter and egfp, and then transfect it into human bladder transitional cell carcinoma biu - 87 cell ; second, based on the changes of the expression of gfp in the biu - 87 cell which induced by the aconitine and hab toxins, the concentration of the hab toxins could be detected

    目的：構建一個含c - fos啟動子和egfp報告基因的pegfp - c - fos重組質粒載體。體外轉染膀胱癌biu - 87細胞后，利用赤潮毒素作用后細胞表達綠色熒光蛋白的變化來檢測赤潮毒素，初步建立一種以細胞為基礎受體水平的赤潮毒素檢測方法。
  2. Materials and methods the mouse, golden hamster and human sperm were incubated with endotoxin in different concentration for different time to get capacitation, respectively, and ar was induced by progesterone after capacitation, then the rates of capacitation and ar were detected by chlortetracycline ( ctc ) and hoechst 33258 fluorescent staining method. the medium was with endotoxin in different concentration in sperm - oocyte fusion step during ivf, then the fertilization rate was observed. the 1 - cell, 2 - cell and zona - free 2 - cell mouse embryos were incubated in the medium with endotoxin, then the rate of blastocysts was recorded

    方法取小鼠精子10份、金黃地鼠精子6份、人新鮮精液標本10份及人冷凍精液標本9份，分別與不同濃度內毒素共孵育進行體外獲能和孕酮誘導的頂體反應，應用金黴素和dna結合的熒光染料hoechest33258雙重熒光染色法檢測精子的獲能率和頂體反應率；小鼠體外受精實驗的精卵結合環節培養液中加入不同濃度的內毒素，觀察受精情況並記錄受精率；取小鼠1 -細胞胚胎、 2 -細胞胚胎和去卵透明帶2 -細胞胚胎，與不同濃度內毒素共孵育進行體外培養，觀察體外發育情況並記錄囊胚率。
  3. The survival - rate of the recovered cells was tested by mtt and fda - pi. therefore, we got the preferable condition of vitrification, i. e., the concentration of cryoprotectant is 60 % and the time of disposal is 15min. under this condition, the value of fda / pi is 46. 43

    凍融后的細胞經過mtt檢測和fda - pi雙熒光染色法檢測，獲得預處理過渡的優化條件，即60濃度的保護劑，預處理15分鐘，此時的fda pi值為46 . 43 。
  4. According to the gene sequence and secondary structure of hcv ns5b, we design the sirnas targeting ns5b gene following with the requirement for sirnas design from tuschl et. al and synthesize it from dharmacon company ; hepg2 cell stably expressing ns5b - egfp protein was trasfected by synthesized sirnas with electroportion, the non - transfected cell and non - specific sirnas transfected cell are c onsidered as control group ; inhibitory effect of sirnas was investigated by fluorescence microscope with dapi dyeing and by semi - quantitative rt - pcr

    然後根據dsrna設計原則，結合nssb基因的序列特徵，藉助生物信息學軟體設計了針對nssb基因的sirnas ，並交由公司化學合成；電穿孔法轉染上述穩定轉染的細胞克隆，同時分別以非特異的sirnas轉染組和空白轉染組為對照， dapi染色后通過熒光顯微鏡和內標化rtpcr檢測，初步證實了化學合成的sirnas可以特異阻斷nssb基因的表達。
  5. Sonoki s, hisamatsu s, kiuchi a. high - performance liquid clromatographic determination of dna base composition with fluorescence detection [ j ]. nucleic acids research 1993, 21 ( 11 ) : 2776

    許化溪，王勝軍，黃錫全，等.高效液相色譜法與熒光法測定細菌染色體堿基組成的比較[ j ] .中華檢驗醫學雜志2000 ， 23 （ 4 ） : 2 - 7
  6. To investigate the potential function of nogo - a involved in neuronal development and differentiation, this study mainly used hippocampal neuron culture model, both in conventional and low - density condition, and immunohistochernical and western blot techniques. nogo - a expression pattern in hippocampal neurons at different stages and its subcellular distribution were explored by using specific antibody raised against nogo - a. pc12, 3t3 cells were also cultured as controls

    為探討nogo a在神經元中的表達與神經元發育分化的關系，本研究藉助了海馬神經元常規培養方法和低密度條件下培養方法，應用針對nogo a的特異性抗體，採用免疫熒光細胞化學染色和westernblot方法，觀察了nogo a在體外培養的大鼠海馬神經元表達分佈模式，和不同培養時間nogo a亞細胞分佈的變化。
  7. A series of validation experiments and genetic studies should be performed for the y - str multiplex system according to the suggestion of the technical work group dna analysis methods ( twgdam ). method we selected four y - str loci, dys434, dys438, dys439, a10 ( y - gata - a10 ) and designed two set of tailed primers to improve the efficiency of the multiplex pcr

    方法選定四個y染色體str基因座，應用加尾序列引物設計策略設計的引物，構建四個基因座的y - str復合擴增體系，建立銀染檢測和熒光檢測方法，依據dna分析技術工作組（ twgdam ）指南進行法醫學可行性研究和群體遺傳研究。
  8. Whether ros contributes equally to its salt sensitibity is unkown. we detected the content of h2o2 and o2 -, by dab and nbt staining respectively, in wild type and sos2 mutant leaves

    本研究利用dab 、 nbt染色以及dcfh - da熒光檢測方法，分別測定了鹽脅迫下野生型和sos2突變體的葉片和根中ros的含量。
  9. In the second trial, this modified discontinuous percoll gradient centrifugation method was introduced to isolate spermatids from the semen of fifteen male infertile patients. then the effect was identified by wright - giemsa stain, flow cytometry analysis, immunocytochemistry and fluorescence in situ hybridization ( fish ). similary, the 22 % percoll fraction contained mostly haploid cells [ ( 91. 85 ? 5. 18 ) % ] ( p < 0. 005 ) and the mean density in this fraction was ( 1. 010 ? 0. 786 ) x 105 / ml

    C法，對15例各種類型不育患者的精液細胞進行分離，並利用瑞姬染色法、流式細胞術、免疫細胞化學和熒光原位雜交oisffi等方法，從細胞形態特徵、 dna倍體、細胞表面標i己與分化抗原，以及原位雜交信號的數目和位置結合細胞核特有的形態等方面加以鑒定。
  10. This study we acquired the coding region of hcv ns5b gene by pcr of hcv full length genome and construct the recombinant plasmid pegep n3 - ns5b ; with the different concentration of g418 in the culture medium, we think the selection concentration of g418 for hepg2 cell is 800 g / ml ; the recombinant plasmid was transfected into hepg2 cell by lipofectamine2000 cells containing stable transformants were selected by the ability of resistance to g418 and isolated with the limited dilution. the stably transfected cell line expressing ns5b - egfp fusion protein was obtained by the detection under fluorescence microscope and rt - pcr

    本研究首先從hcv基因組中擴增出nssb基因，構建了nssb基因與報告分子egfp （增強型綠色熒光蛋白）基因的融合基因真核表達質粒pegfpn30 ；通過含有不同g418濃度梯度的培養液培養hepgz細胞，確定了篩選用g4181作濃度為800pg ml ；利用脂質體法將該重組質粒轉染hepgz細胞，經過有限稀釋法和g4壓力選擇，應用熒光顯微鏡和rtpcr檢測，獲得可穩定表達nssbegfp融合蛋白的hepgz細胞克隆。
  11. This review focuses on the techniques and methods that are currently used for the detection of apoptosis, such as morphological observation, fluorescence staining, dna ladder, caspase activity, etc, and on the evaluation of the advantages and limitations of these methods

    本文綜述了常用的細胞凋亡檢測技術與方法的基本原理，如形態學觀察、熒光素染色、 dna階梯、半胱氨酸天冬氨酸蛋白酶活性的檢測等，並對各種方法的優點及局限性做一簡要比較。
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