熒光蛋白基因 的英文怎麼說

中文拼音 [yíngguāngdànbáijīyīn]
熒光蛋白基因 英文
egfp
  • 熒 : 形容詞[書面語]1. （光亮微弱的樣子） glimmering 2. （眼光迷亂; 疑惑） dazzled; perplexed
  • 光 : Ⅰ名詞1 （照耀在物體上、使人能看見物體的一種物質） light; ray 2 （景物） scenery 3 （光彩; 榮譽） ...
  • 蛋 : 名詞1. （鳥類或龜、蛇類所產的卵） egg 2. （像蛋形的東西） an egg-shaped thing 3. （辱罵之詞）
  • 白 : Ⅰ形容詞1 （似雪的顏色） white 2 （清楚; 明白; 弄明白） clear 3 （空的; 沒加他物的） pure; clear; ...
  • 因 : Ⅰ動詞[書面語] （沿襲） follow; carry on Ⅱ介詞1 [書面語] （憑借; 根據） on the basis of; in accord...
  • 蛋白 : 1. （卵中透明的膠狀物質） egg white; albumen; gary2. [生物化學] （蛋白質） protein
  1. First, to construct a recombinant plasmid pegfp - c - fos with c - fos promoter and egfp, and then transfect it into human bladder transitional cell carcinoma biu - 87 cell ; second, based on the changes of the expression of gfp in the biu - 87 cell which induced by the aconitine and hab toxins, the concentration of the hab toxins could be detected

    目的：構建一個含c - fos啟動子和egfp報告基因的pegfp - c - fos重組質粒載體。體外轉染膀胱癌biu - 87細胞后，利用赤潮毒素作用后細胞表達綠色熒光蛋白的變化來檢測赤潮毒素，初步建立一種以細胞為基礎受體水平的赤潮毒素檢測方法。
  2. In this study, in order to examine the dynamics of tip [ ca2 + ] i together with the dynamics of tip - localized f - actin in vivo, a kind of double labeled material was constructed. the ca2 + and actin microfilaments of arabidopsis pollen tubes were labeled by cameleon and gfp - mtalin respectively

    本研究以擬南芥花粉管為材料，通過轉基因技術，將分別標記ca ~ （ 2 + ）和微絲的兩種熒光蛋白cameleon與gfp - mtalin在花粉管中同時表達，實現活體花粉管中ca ~ （ 2 + ）與微絲的同時標記。
  3. Green fluorescent protein ( gfp ) gene was conjugated to the 3 " end of the pap gene in order to screen easily of the transgenic cotton plants. the combined gene was cloned into plant expression vector pbi121 and then transformed. about 5000 seeds of the transgenic cotton were obtained and the some seedlings of the transgenic cotton could give a bright green fluorescence in the dark condition when the cotton seedlings were irradiated with ultraviolet rays

    為了便於轉基因棉花後代的篩選，在pap基因的3 』端融入了綠色熒光蛋白gfp ）基因，然後將融合基因克隆在植物表達載體pbi121上，再進行遺傳轉化，得轉基因棉花種子5000餘粒，將種子播種長到于葉展開時，先在黑暗中用紫外燈照射，查找表現綠色熒光的幼苗，然後再用地高辛（ dig ）標記的pap基因特異性探針對這些棉花進行點雜交，最後發現有8株棉花表現陽性反應，說明pap基因的確己經轉到了棉花的基因組中，其棉花黃萎病的抗性鑒定正在進行之中。
  4. It was confirmed by restriction enzyme digestion analysis that egfp fusion expression plasmids of scfvs were successfully constructed

    的序列正確，經酶切鑒定證實成功地構建了綠色熒光蛋白基因融合表達載體。
  5. Improvement of genetic transformation system by using gfp as reporter gene on pepper

    應用綠色熒光蛋白報告基因優化辣椒的遺傳轉化體系
  6. Production of transgenic embryos expressing green fluorescent protein by nuclear transfer from different types of somatic cells in pig

    不同類型的轉基因細胞為核供體生產豬的轉綠色熒光蛋白基因克隆胚胎
  7. The symbiosis between mesorhizobium huakuii and astragalus sinicus is a chinese - characteristic symbiotic nitrogen fixation system, while molecular genetic study on its early symbiotic interaction is still at primary stage

    本文對新型報告基因?綠色熒光蛋白基因在華癸中生根瘤菌-紫雲英共生固氮體系早期結瘤階段分子遺傳學中的應用進行了探索性研究。
  8. Green fluorescent protein is widely applied in researches of modern life science, such as gene product moved - process in vivo, protein localization, drug screening and preliminary selection of transgenic individual as a molecular marker

    綠色熒光蛋白這種獨特的生物學特性，使其作為報道基因在現代生命科學研究領域中，如細胞內基因產物的動態過程，蛋白質在細胞內的定位，藥物的篩選，以及轉基因個體的初步鑒定等等有著廣泛的應用。
  9. Improvement of adcmv - gfp gene transfection efficiency induced by heavy - ion beam irradiation on murine melanoma cells

    離子束輻射對用帶有綠色熒光蛋白基因的缺陷性腺病毒
  10. 4, an intron sequence was also inserted upstream of gfpmut3 and its six reading frame could all be stopped, which could guarantee gfp translation in right reading frame

    將藍色熒光蛋白基因bfp克隆到pet - 11c上，轉化bl21 （ de3 ）后實現了bfp在大腸桿菌中的誘導表達。
  11. Based on the foundation research of the interaction of virus and host actin, we recombine gfp gene - a reporter gene - with 5c actin gene of drosophila melanogaster, a gfp - actin fusion gene was obtained

    本文在總結前人關于病毒與宿主肌動蛋白相互作用的研究的基礎上，利用綠色熒光蛋白基因為標記基因，與果蠅肌動蛋白基因5cactin基因融合，構成gfp - actin融合基因。
  12. 2. expression of gfp gene in b. thuringiensis strain green fluorescent protein gene is a very useful report gene which could be detected easily. it can be used in the detection of b. thuringiensis environmental release and study of the insecticidal mechanism

    綠色熒光蛋白基因在蘇雲金芽胞桿菌中的表達綠色熒光蛋白基因是一種容易檢測的報告基因，可用於蘇雲金芽胞桿菌制劑施用后的環境監測，殺蟲機理等方面的研究。
  13. In the research of transgenic fish, green fluorescent protein gene was sub - cloned to downstream of carp p - actin gene promoter that was cloned in pucusa by molecular recombination technology. thus pagfp plasmid was constructed successfully. the recombination was determined by digestion of restriction enzyme and sequencing

    實驗通過分子重組技術，採用定向克隆法將綠色熒光蛋白基因亞克隆到puc118a上鯉魚-肌動蛋白基因啟動子下游，構建成能在真核生物體內表達的表達載體pagfp ，經雙酶酶切法序列鑒定后，回收帶啟動子和目的基因片段。
  14. Green fluorescent protein has several good characters. under excitation of long uv light or blue light, it emits green fluorescence without requiring any exogenous substrates and cofactors. gfp gene expression can be used to monitor gene expression and protein localization in living cells and organisms. this is a development of revolutionary significance. the dna sequence of this gene can be re - engineered by mutagenesis and the gfp will get improved fluorescent properties. the applications of gfp will be wider and wider

    綠色熒光蛋白具有優良的特性，在藍光或長紫外光的激發下，不需要任何外源底物或內源輔助因子的參入就能發出綠色熒光.綠色熒光蛋白基因的表達可用來監控活細胞或生物體中基因表達和蛋白質的定位.這是一個革命性的進展.而且，對基因dna序列的改造有可能使綠色熒光蛋白的發光特性更加優良，從而其應用范圍會更加廣泛
  15. Isolation, culture and characterization of neural stem cells from newborn rat spinal cords

    綠色熒光蛋白轉基因小鼠神經幹細胞的培養與鑒定
  16. The expression and localization of wild - type p53 - gfp fused gene on human high - metastasis hepatocellular carcinoma cell line

    熒光蛋白融合基因在高轉移人肝癌細胞中的表達與定位
  17. The ha 122 protein, when fused to gfp was observed in the nuclei of h. armigera cells, but only in conjunction with wild type hasnpv infection

    將ha122與綠色熒光蛋白（ gfp ）基因融合，在昆蟲細胞中瞬時表達。當有病毒感染同時發生時， gfp信號定位在核內。
  18. In order to get the soluble recombinant eo protein and inspect the protein expression status convinently, the egfp and eo gene were ligated into baculovirus transfer vector. with the co - transfecting sf9 cells of baculovirus recombinant transfer vector and linearized viral dna, and plaque purification in the posttransfection procedure, the pure recombinant baculovirus were harvested, which infected the sf9 cells for amplifying to generate a p - l stock. in the meantime, the fluorescence microscopy detection indicated expressed egfp protein to confirm the heterogenous protein expression of recombinant baculovirus. the pi - stock from a pure plaque was used to generate a high liter p - 2 stock, which was determined in liter as 1. 14 107pfu / ml by performing a plaque assay. when a volume of p - 2 stock infected the sf9 cells with moi 5 - 10 for expression, the strong fluorescence was obeserved on the day 3 of postinfection

    此外，為了得到可溶性重組eo蛋白並便於觀察重組蛋白的表達情況，我們將egfp基因與eo基因相連插入昆蟲桿狀病毒轉移載體中，與線性桿狀病毒dna共轉染sf9細胞后通過噬斑純化得到純的重組桿狀病毒，將其感染sf9細胞制備p1種子液，同時用熒光顯微鏡觀察綠色熒光蛋白的表達情況剔除表達效果差的重組桿狀病毒。再用p1種子液感染sf9細胞制備高效價的p2種子液。通過病毒液的梯度稀釋和噬斑測定，確定p2種子液的病毒滴度達1 . 14 10 ~ 7pfu ml 。
  19. In order to understand the apoplast cam gene and the relationship between the apoplast cam and cam isoforms, we intended to get transgenic plants harboring soybean calmodulin isoform genes ( scams ) fused to gfp as a reporter and study the subcellular localization of scams

    為了進一步利用分子生物學方法在基因水平上研究胞外cam的存在，同時研究胞外cam與cam亞型之間的關系，本論文利用綠色熒光蛋白（ gfp ）作為報告基因研究了大豆cam基因家族（ scams ）的亞細胞定位。
  20. This study we acquired the coding region of hcv ns5b gene by pcr of hcv full length genome and construct the recombinant plasmid pegep n3 - ns5b ; with the different concentration of g418 in the culture medium, we think the selection concentration of g418 for hepg2 cell is 800 g / ml ; the recombinant plasmid was transfected into hepg2 cell by lipofectamine2000 cells containing stable transformants were selected by the ability of resistance to g418 and isolated with the limited dilution. the stably transfected cell line expressing ns5b - egfp fusion protein was obtained by the detection under fluorescence microscope and rt - pcr

    本研究首先從hcv基因組中擴增出nssb基因，構建了nssb基因與報告分子egfp （增強型綠色熒光蛋白）基因的融合基因真核表達質粒pegfpn30 ；通過含有不同g418濃度梯度的培養液培養hepgz細胞，確定了篩選用g4181作濃度為800pg ml ；利用脂質體法將該重組質粒轉染hepgz細胞，經過有限稀釋法和g4壓力選擇，應用熒光顯微鏡和rtpcr檢測，獲得可穩定表達nssbegfp融合蛋白的hepgz細胞克隆。
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