熒光顯微法 的英文怎麼說

中文拼音 [yíngguāngxiǎnwéifǎ]
熒光顯微法 英文
fluorescence microscopy
  • 熒 : 形容詞[書面語]1. （光亮微弱的樣子） glimmering 2. （眼光迷亂; 疑惑） dazzled; perplexed
  • 光 : Ⅰ名詞1 （照耀在物體上、使人能看見物體的一種物質） light; ray 2 （景物） scenery 3 （光彩; 榮譽） ...
  • 顯 : Ⅰ形容詞1 （明顯） apparent; obvious; noticeable; evident 2 （有名聲有權勢的） illustrious and inf...
  • 法 : Ⅰ名詞1 （由國家制定或認可的行為規則的總稱） law 2 （方法; 方式） way; method; mode; means 3 （標...
  • 顯微 : microadiography
  1. Recombination gene was cut - down and introduced into the nuclei of oocytes or the cytoplasm of goldfish at one - cell stage via microinjection. the results as follows : ( 1 ) fluorescence was observed from embryo under suitable uv light after microinjection 36 hours. the fluorescent ratio of gastrula embryo period was up to 25 %

    採用顯微注射法將這種重組基因轉化1細胞期的金魚受精卵，實驗結果如下： （ 1 ）顯微注射后，根據胚胎發育分期，胚胎在顯微注射后36小時開始能在紫外燈下觀察到熒光，原腸期發熒光的胚胎比例為25 ，後期發育熒光率逐漸下降，肌肉效應期后又相對穩定。
  2. We used fission yeast schizosaccharomyces pombe ( s. pombe ), an unicellular eukaryotic organism, as research material. electroporation was adopted to load ca2 + fluorescent indicator into yeast cell and under the laser scanning confocal microscopy ( lscm ), we observed cytosolic ca2 + distribution and relative content as well as fluorescence intensity of gfp - cam in different phases of cell cycle of yeast cell. flow cytometry provided a way of determining the relative dna content of populations of fission yeast

    本文以單細胞的真核模式生物裂殖酵母（ schizosaccharomycespombe ）為研究材料，通過激光掃描共聚焦顯微鏡觀察酵母細胞胞質內游離ca ~ （ 2 + ）的分佈及相對濃度，以及不同周期時相細胞中gfp - cam的熒光強度變化，並採用細胞流式法對酵母細胞的相對dna含量進行測定以確定細胞所處周期時相。
  3. On the backgrounds of researches inside and outside country, and cooperating experiments with theories analyses, the influence of different processing technology parameters and different sbs modifier sorts on the sbs modified asphalts " properties has been studied. at the same time, their microstructure are observed through fluorescence optical microscopy and scanning electronic microscopy, thus to direct modified asphalt production. on the above conclusion ' s basement, analysing some disadvantages of the storage stability test of sbs modified asphalt in the current specification, a new storage stability test apparatus is developed

    本文在參考國內外研究的基礎上，採用理論、試驗相結合的方法，研究加工工藝參數以及改性劑種類等對sbs改性瀝青性能的影響，並通過熒光顯微鏡、掃描電鏡分析其微觀形態，從而指導sbs改性瀝青的生產；在此基礎上，分析我國現行規范用來評價sbs改性瀝青儲存穩定性方面的不足，開發了新的試驗儀，根據動態剪切流變試驗結果和微觀狀態分析，提出一個新的指標? ?離析率r _ s來評價sbs改性瀝青的儲存穩定性；最後，針對不穩定的改性瀝青提出改善措施，研究證明摻加增容劑和穩定劑是行之有效的方法。
  4. We investigated the distribution of the heterotrophic bacteria with the epifluorescence microscope and measured the bacterial production with the tritiated tymicline incorporation method, and we investigated the correlation between the heterotrophic bacteria and chlorophyll, inorganic nitrogen also. there was distinct spatial distribution of the bacterial biomass in the east china sea and the yellow sea during fall and spring

    本文利用表面熒光顯微鏡觀測計數法和[甲基- 3h ]胸腺嘧啶示蹤法對春秋兩季節我國黃、東海異養細菌生態分佈及其生產力狀況，以及異養細菌及其生產力與浮游植物葉綠素、無機氮鹽之間的關系進行了研究。
  5. The epitaxial growths of ingaas / gaas / algaas fundamental material and the fabrication of 45 - deflector are extensively studied in our work. some measuring methods are used to evaluate the growth quality of our grown structure by pl, cv, x - ray double crystal diffraction, sem etc. property analysis are provided for it

    利用高能電子衍射、電化學c - v 、掃描電鏡（ sem ） 、 x射線雙晶衍射儀、光熒光譜儀（ pl ） 、原子力顯微鏡等多種方法對制備的器件進行了檢測，同時對實驗結果進行了必要的分析。
  6. In this paper, wheat cultivars lovrin 10 ( resistant ) and 5389 ( susceptible ) were selected as materials in this system. the mesophyll protoplasts of wheats ( mpw ) were isolated using cellulase and pectolase digestion. by the indirect immune fluorescent labeling, microtubules ( mts ) pattern in mpw were showed clearly under the confocal laser scanning microscope ( clsm )

    本試驗以抗（洛夫林10 ） 、感（ 5389 ）不同的兩小麥（ triticumaestivuml . ）品種為材料，採用酶解法制備小麥葉肉原生質體，利用間接免疫熒光方法結合激光共聚焦掃描顯微技術對小麥葉肉細胞原生質體的微管骨架進行了清晰的標記，並探索了微管骨架標記的影響因素。
  7. Methods according to theory of specific binding of antigen and antibody, at first the anti - a monoclonal antibody ( ma ) and anti - bma were labeled with the fluorescent, then fluorescent - labeled antibodies ( fla ) were bound with corresponding biological material ( such as bloodstain ) in the optimum condition, finally the abo blood type of bloodstain was determined under microscope fluorescent

    方法根據抗原抗體特異性結合的原理，首先對抗a 、抗b單克隆抗體進行熒光標記，然後使熒光標記抗體與相應抗原（血痕）在最佳條件下結合，最後熒光顯微鏡鏡檢，判定血痕的血型。
  8. The characteristics of this method are : a, directly counting cell number without the influence of the metabolic state of the cells ; b, discrimination of target cells from effector cells in cell - mediated cytotoxicity assay ; c, less treatment step, and free - radioactivity ; d, high sensitivity and reliability. 2, using the above assay, immunofluorescent labeled technique, and flow cytometry, the pbmc proliferation, apoptosis, necrosis, cell cycle, activation, cytokines and membrane marker were detected. the results showed that the number of pbmc reduced, but the activity of pbmc increased dose - dependently ; the reduction of cell number resulted from necrosis and apoptosis ; the supernatant of k562 cell lines were not able to block the cell cycle, but to promote it ; the ratio of t cell subset and the expression of thl and th2 cytokines increased

    結合以上創建的方法和免疫熒光流式細胞術，用k562細胞株可溶性分泌物（上清）對外周血單個核細胞（ pbmc ）進行培養以模擬體內微環境，然後分別從細胞增殖、凋亡、壞死、細胞周期、活性、細胞因子和表面抗原表達等方面進行研究，結果發現用腫瘤上清培養的pbmc細胞數量下降明顯，但同時對其有激活作用，且呈劑量依賴性；細胞數的下降主要是由細胞壞死和凋亡引起的，腫瘤上清對細胞周期沒有阻斷作用，反而略有促進作用； t細胞亞群比例增加，並促進表達th1 、 th2細胞因子。
  9. The approaches used in the study included : observing the microstructure and ultrastructure of the cell line of colossoma brachypomum ( cbt ) and the cell line of carp ( cp ) stressed low temperatures under fluoroscopy and tem ; analysis of dna damage in the cultured cells under temperatures stress by dna gel electrophoresis

    本研究採用的主要實驗方法：通過熒光顯微觀察、電鏡超微結構觀察確定cbt （淡水白鯧臀鰭細胞）和cp （草魚胚胎細胞）在低溫處理后的顯微與超微結構的變化。應用dna電泳分析細胞dna在低溫處理后的斷裂現象。
  10. First, glass slides having been rinsed will be treated with nh3h2o, aminosilane and aldehyde. second, the quality of pretreatment surface of glass slides can be tested through methods of fluorescence and afm microscope. in the end, the characteristic of probe immobile ratio for oligonucleotide on glass surface is obtained through researching the internal relation of these two methods

    實驗選用表面平整的德國玻片，將清洗好的玻片分別進行羥基化、氨基化、醛基化，採用熒光法和原子力顯微鏡法分別檢測玻片表面預處理質量，研究兩種檢測方法之間的內在聯系，從而確定表徵玻片表面寡核苷酸探針固定率的方法。
  11. Test method for enumeration of aquatic bacteria by epifluorescence microscopy counting procedure

    按熒光顯微鏡計算程序進行水生菌計數的試驗方法
  12. The composition, structure, and properties of the as prepared composite films have been characterized in detail by uv - vis, ftir, and x - ray photoelectron spectra, ellipsometry, scanning electron microscopy, atomic force microscopy, transmission electron microscopy, fluorescence spectroscopy, and standard four - probe technique

    採用uv - vis光譜、 ftir光譜、 x -射線光電子能譜、橢圓光度法、掃描電子顯微鏡、原子力顯微鏡、透射電子顯微鏡、熒光光譜和標準四探針技術對所制備的納米復合膜進行了組成、結構和性能表徵。
  13. According to the gene sequence and secondary structure of hcv ns5b, we design the sirnas targeting ns5b gene following with the requirement for sirnas design from tuschl et. al and synthesize it from dharmacon company ; hepg2 cell stably expressing ns5b - egfp protein was trasfected by synthesized sirnas with electroportion, the non - transfected cell and non - specific sirnas transfected cell are c onsidered as control group ; inhibitory effect of sirnas was investigated by fluorescence microscope with dapi dyeing and by semi - quantitative rt - pcr

    然後根據dsrna設計原則，結合nssb基因的序列特徵，藉助生物信息學軟體設計了針對nssb基因的sirnas ，並交由公司化學合成；電穿孔法轉染上述穩定轉染的細胞克隆，同時分別以非特異的sirnas轉染組和空白轉染組為對照， dapi染色后通過熒光顯微鏡和內標化rtpcr檢測，初步證實了化學合成的sirnas可以特異阻斷nssb基因的表達。
  14. Test method rapid enumeration of bacteria in electronics - grade purified water systems by direct - count epifluorescence microscopy

    用直接計數淺熒光顯微鏡進行電子級凈化水系統中細菌的快速記數的試驗方法
  15. In this dissertation, the plasmids containing 5s promoter were transfected into cho cells and the transcription sites of rna polymerase and its transcripts were detected by fluorescence in situ hybridization to dna and rna, respectively

    本實驗以中國倉鼠卵巢細胞（ cho ）為實驗材料，利用基因轉染、熒光原位雜交並結合激光共聚焦顯微鏡觀察的方法，在dna和rna水平上分別對rna聚合酶的轉錄位點和轉錄子的分佈進行了檢測。
  16. Aqueous fluid volume and [ c1 ~ j were assayed in samples withdrawn by micropipettes. intraocular pressure ( top ), pressure - dependent outflow, and anterior chamber compliance were determined from pressure measurements in response to pulsed and continuous fluid infusions into the anterior chamber using micropipettes. result : in wildtype mice ( gdi genetic background, age 4 - 6 weeks ), iop was 16. 0 ? 0. 4 mmhg, aqueous fluid volume was 7. 2 ? 0. 3 ul, aqueous fluid production was 3. 6 ? 0. 2 ul / hr, aqueous fluid outflow was 0. 36 ? 0. 06 ul / hr / mmhg, and anterior chamber compliance was 0. 036 ? 0. 006 ul / mmhg ( mean ? se, 8 - 10 eyes )

    實驗方法包括：將熒光物質用電離子滲透的方法穿透角膜導入活體小鼠的前房中，然後應用共聚焦顯微鏡根據熒光強度變化測量房水生成率；通過顯微注射針吸取房水檢測房水容積和氯離子濃度；顯微玻璃管刺入前房測量眼內壓，並將生理鹽水分別以連續和脈沖兩種方式注入前房，測量房水間隙的順應性和房水排出與眼內壓的相關性。
  17. It was obvious that the proposed analytical method of the air carrier in microfluidic system were not suitable for the spectrophotometric, electrochemical and fluorescence detection. however, when applied in chemiluminescence detection, the distinguished advantages of this method could be shown enough

    很顯然，微流控系統中用空氣作載流的分析方法不適于分光檢測、電化學檢測以及熒光檢測，但用化學發光檢測時則顯示出獨特的優勢。
  18. In order to enrich the content of the anatomy and histology about andrias davidianus, obtain new data about systematic position and blood circulation physiology and also provide reference for comparative anatomy and vertebrate evolution reseach, we studied the anatomy and histology about andrias davidianus by means of normal inject method, normal paraffin section method, microcopy system and digital camera

    本研究主要採用常規血管注射法，常規石蠟切片法，以及光學顯微照相系統和熒光顯微數碼照相系統對中國大鯢（ andriasdavidianus ）的循環系統進行了比較詳細的解剖學觀察研究，並對其心臟及血管進行了組織學研究。目的在於豐富中國大鯢解剖學和組織學的資料，為中國大鯢分類地位的探討和血液循環生理研究提供解剖學依據，同時也為比較解剖學和脊椎動物的進化提供解剖學依據。
  19. This new method will bring significant developments in studying the principles of stomatal movement, and other quick movement in plants, c ) guard cells are incubated with ph dependent fluorescent chemical probe " bcecf am " and excited at 488nm, the fluorescent emission ratio method ( 520nm / 640nm ) is employed with laser scanning confocal microscopy, about 0. 4 ph unit increase in guard cell vacuoles is observed during stomatal closure that is induced by aba

    本發展為保衛細胞與其它小細胞液泡的進一步研究提供了新思路。 c ）本工作通過激光共聚焦顯微術配合ph熒光探針bcecfam的單激發（ 488nm ）雙發射的熒光比值法（ 525nm 640nm ）觀察到，用aba處理的表皮條上的開放態氣孔在關閉過程中其保衛細胞液泡內ph有一約0 . 4單位的上升。
  20. This study we acquired the coding region of hcv ns5b gene by pcr of hcv full length genome and construct the recombinant plasmid pegep n3 - ns5b ; with the different concentration of g418 in the culture medium, we think the selection concentration of g418 for hepg2 cell is 800 g / ml ; the recombinant plasmid was transfected into hepg2 cell by lipofectamine2000 cells containing stable transformants were selected by the ability of resistance to g418 and isolated with the limited dilution. the stably transfected cell line expressing ns5b - egfp fusion protein was obtained by the detection under fluorescence microscope and rt - pcr

    本研究首先從hcv基因組中擴增出nssb基因，構建了nssb基因與報告分子egfp （增強型綠色熒光蛋白）基因的融合基因真核表達質粒pegfpn30 ；通過含有不同g418濃度梯度的培養液培養hepgz細胞，確定了篩選用g4181作濃度為800pg ml ；利用脂質體法將該重組質粒轉染hepgz細胞，經過有限稀釋法和g4壓力選擇，應用熒光顯微鏡和rtpcr檢測，獲得可穩定表達nssbegfp融合蛋白的hepgz細胞克隆。
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