熒光顯微鏡法 的英文怎麼說

中文拼音 [yíngguāngxiǎnwéijìngfǎ]
熒光顯微鏡法 英文
fluorescence microscopy
  • 熒 : 形容詞[書面語]1. （光亮微弱的樣子） glimmering 2. （眼光迷亂; 疑惑） dazzled; perplexed
  • 光 : Ⅰ名詞1 （照耀在物體上、使人能看見物體的一種物質） light; ray 2 （景物） scenery 3 （光彩; 榮譽） ...
  • 顯 : Ⅰ形容詞1 （明顯） apparent; obvious; noticeable; evident 2 （有名聲有權勢的） illustrious and inf...
  • 鏡 : Ⅰ名詞1 （鏡子） looking glass; mirror 2 （幫助視力或做光學實驗的器具） lens; glass 3 （姓氏） a s...
  • 法 : Ⅰ名詞1 （由國家制定或認可的行為規則的總稱） law 2 （方法; 方式） way; method; mode; means 3 （標...
  • 顯微鏡 : microscope
  • 顯微 : microadiography
  1. We used fission yeast schizosaccharomyces pombe ( s. pombe ), an unicellular eukaryotic organism, as research material. electroporation was adopted to load ca2 + fluorescent indicator into yeast cell and under the laser scanning confocal microscopy ( lscm ), we observed cytosolic ca2 + distribution and relative content as well as fluorescence intensity of gfp - cam in different phases of cell cycle of yeast cell. flow cytometry provided a way of determining the relative dna content of populations of fission yeast

    本文以單細胞的真核模式生物裂殖酵母（ schizosaccharomycespombe ）為研究材料，通過激光掃描共聚焦顯微鏡觀察酵母細胞胞質內游離ca ~ （ 2 + ）的分佈及相對濃度，以及不同周期時相細胞中gfp - cam的熒光強度變化，並採用細胞流式法對酵母細胞的相對dna含量進行測定以確定細胞所處周期時相。
  2. On the backgrounds of researches inside and outside country, and cooperating experiments with theories analyses, the influence of different processing technology parameters and different sbs modifier sorts on the sbs modified asphalts " properties has been studied. at the same time, their microstructure are observed through fluorescence optical microscopy and scanning electronic microscopy, thus to direct modified asphalt production. on the above conclusion ' s basement, analysing some disadvantages of the storage stability test of sbs modified asphalt in the current specification, a new storage stability test apparatus is developed

    本文在參考國內外研究的基礎上，採用理論、試驗相結合的方法，研究加工工藝參數以及改性劑種類等對sbs改性瀝青性能的影響，並通過熒光顯微鏡、掃描電鏡分析其微觀形態，從而指導sbs改性瀝青的生產；在此基礎上，分析我國現行規范用來評價sbs改性瀝青儲存穩定性方面的不足，開發了新的試驗儀，根據動態剪切流變試驗結果和微觀狀態分析，提出一個新的指標? ?離析率r _ s來評價sbs改性瀝青的儲存穩定性；最後，針對不穩定的改性瀝青提出改善措施，研究證明摻加增容劑和穩定劑是行之有效的方法。
  3. We investigated the distribution of the heterotrophic bacteria with the epifluorescence microscope and measured the bacterial production with the tritiated tymicline incorporation method, and we investigated the correlation between the heterotrophic bacteria and chlorophyll, inorganic nitrogen also. there was distinct spatial distribution of the bacterial biomass in the east china sea and the yellow sea during fall and spring

    本文利用表面熒光顯微鏡觀測計數法和[甲基- 3h ]胸腺嘧啶示蹤法對春秋兩季節我國黃、東海異養細菌生態分佈及其生產力狀況，以及異養細菌及其生產力與浮游植物葉綠素、無機氮鹽之間的關系進行了研究。
  4. The epitaxial growths of ingaas / gaas / algaas fundamental material and the fabrication of 45 - deflector are extensively studied in our work. some measuring methods are used to evaluate the growth quality of our grown structure by pl, cv, x - ray double crystal diffraction, sem etc. property analysis are provided for it

    利用高能電子衍射、電化學c - v 、掃描電鏡（ sem ） 、 x射線雙晶衍射儀、光熒光譜儀（ pl ） 、原子力顯微鏡等多種方法對制備的器件進行了檢測，同時對實驗結果進行了必要的分析。
  5. Methods according to theory of specific binding of antigen and antibody, at first the anti - a monoclonal antibody ( ma ) and anti - bma were labeled with the fluorescent, then fluorescent - labeled antibodies ( fla ) were bound with corresponding biological material ( such as bloodstain ) in the optimum condition, finally the abo blood type of bloodstain was determined under microscope fluorescent

    方法根據抗原抗體特異性結合的原理，首先對抗a 、抗b單克隆抗體進行熒光標記，然後使熒光標記抗體與相應抗原（血痕）在最佳條件下結合，最後熒光顯微鏡鏡檢，判定血痕的血型。
  6. The approaches used in the study included : observing the microstructure and ultrastructure of the cell line of colossoma brachypomum ( cbt ) and the cell line of carp ( cp ) stressed low temperatures under fluoroscopy and tem ; analysis of dna damage in the cultured cells under temperatures stress by dna gel electrophoresis

    本研究採用的主要實驗方法：通過熒光顯微觀察、電鏡超微結構觀察確定cbt （淡水白鯧臀鰭細胞）和cp （草魚胚胎細胞）在低溫處理后的顯微與超微結構的變化。應用dna電泳分析細胞dna在低溫處理后的斷裂現象。
  7. First, glass slides having been rinsed will be treated with nh3h2o, aminosilane and aldehyde. second, the quality of pretreatment surface of glass slides can be tested through methods of fluorescence and afm microscope. in the end, the characteristic of probe immobile ratio for oligonucleotide on glass surface is obtained through researching the internal relation of these two methods

    實驗選用表面平整的德國玻片，將清洗好的玻片分別進行羥基化、氨基化、醛基化，採用熒光法和原子力顯微鏡法分別檢測玻片表面預處理質量，研究兩種檢測方法之間的內在聯系，從而確定表徵玻片表面寡核苷酸探針固定率的方法。
  8. Test method for enumeration of aquatic bacteria by epifluorescence microscopy counting procedure

    按熒光顯微鏡計算程序進行水生菌計數的試驗方法
  9. The composition, structure, and properties of the as prepared composite films have been characterized in detail by uv - vis, ftir, and x - ray photoelectron spectra, ellipsometry, scanning electron microscopy, atomic force microscopy, transmission electron microscopy, fluorescence spectroscopy, and standard four - probe technique

    採用uv - vis光譜、 ftir光譜、 x -射線光電子能譜、橢圓光度法、掃描電子顯微鏡、原子力顯微鏡、透射電子顯微鏡、熒光光譜和標準四探針技術對所制備的納米復合膜進行了組成、結構和性能表徵。
  10. According to the gene sequence and secondary structure of hcv ns5b, we design the sirnas targeting ns5b gene following with the requirement for sirnas design from tuschl et. al and synthesize it from dharmacon company ; hepg2 cell stably expressing ns5b - egfp protein was trasfected by synthesized sirnas with electroportion, the non - transfected cell and non - specific sirnas transfected cell are c onsidered as control group ; inhibitory effect of sirnas was investigated by fluorescence microscope with dapi dyeing and by semi - quantitative rt - pcr

    然後根據dsrna設計原則，結合nssb基因的序列特徵，藉助生物信息學軟體設計了針對nssb基因的sirnas ，並交由公司化學合成；電穿孔法轉染上述穩定轉染的細胞克隆，同時分別以非特異的sirnas轉染組和空白轉染組為對照， dapi染色后通過熒光顯微鏡和內標化rtpcr檢測，初步證實了化學合成的sirnas可以特異阻斷nssb基因的表達。
  11. Test method rapid enumeration of bacteria in electronics - grade purified water systems by direct - count epifluorescence microscopy

    用直接計數淺熒光顯微鏡進行電子級凈化水系統中細菌的快速記數的試驗方法
  12. In this dissertation, the plasmids containing 5s promoter were transfected into cho cells and the transcription sites of rna polymerase and its transcripts were detected by fluorescence in situ hybridization to dna and rna, respectively

    本實驗以中國倉鼠卵巢細胞（ cho ）為實驗材料，利用基因轉染、熒光原位雜交並結合激光共聚焦顯微鏡觀察的方法，在dna和rna水平上分別對rna聚合酶的轉錄位點和轉錄子的分佈進行了檢測。
  13. Aqueous fluid volume and [ c1 ~ j were assayed in samples withdrawn by micropipettes. intraocular pressure ( top ), pressure - dependent outflow, and anterior chamber compliance were determined from pressure measurements in response to pulsed and continuous fluid infusions into the anterior chamber using micropipettes. result : in wildtype mice ( gdi genetic background, age 4 - 6 weeks ), iop was 16. 0 ? 0. 4 mmhg, aqueous fluid volume was 7. 2 ? 0. 3 ul, aqueous fluid production was 3. 6 ? 0. 2 ul / hr, aqueous fluid outflow was 0. 36 ? 0. 06 ul / hr / mmhg, and anterior chamber compliance was 0. 036 ? 0. 006 ul / mmhg ( mean ? se, 8 - 10 eyes )

    實驗方法包括：將熒光物質用電離子滲透的方法穿透角膜導入活體小鼠的前房中，然後應用共聚焦顯微鏡根據熒光強度變化測量房水生成率；通過顯微注射針吸取房水檢測房水容積和氯離子濃度；顯微玻璃管刺入前房測量眼內壓，並將生理鹽水分別以連續和脈沖兩種方式注入前房，測量房水間隙的順應性和房水排出與眼內壓的相關性。
  14. Especially, the nano - iron and nano - tungsten sols as well as their powders were characterized in detail by xrd, ft - ir, uv - vis, xfs, tem, sem, edax and so on. another new method is put forward firstly to estimate the addition of dispersants and its effect by uv - vis

    重點選取納米鐵乙醇溶膠和納米鎢乙醇溶膠以及其中的納米顆粒進行了詳細表徵，所涉及的測試方法包括x射線衍射、傅立葉紅外光譜、紫外-可見光譜、熒光光譜、透射電子顯微鏡及選區衍射、掃描電子顯微鏡及微區能譜分析等等。
  15. In order to investigate the role of mannose receptor ( mr ) of human sperm, the zona free hamster eggs were pre - incubated with purified mr ( pmr ) isolated from motile human sperm by mannose - agarose gel affinity chromatography. the ultrastuctural alteration and cortical granule exocytosis of the eggs were then observed by transmissian electron microscope and tritc - lca immunofluorescence microscope, respectively. the mice were immunized with pmr and the antiserum was raised. after capacitation and induction of the acrosome reaction, the human spermatozoa and oocytes were incubated with the antiserum. then the sperm penetration assay was undertaken

    為了進一步探討人精於mr在精卵融合中的作用，本文採用改良后的甘露糖-瓊脂糖凝膠親和層析法分離純化人精子mr ，並將提純的人精子甘露糖受體（ purifiedmannosereceptor ， pmr ）作用於去透明帶的金黃地鼠卵母細胞，運用透射電子顯微鏡技術和羅丹明偶聯的兵豆凝集素（ tritc - lca ）免疫熒光標記技術觀察pmr對卵子的影響。
  16. According to the significant improvement in properties of ions and particles doped perovskite, tb and cnt doped pt materials were prepared, and the properties studied. tb and cnt doped pt powders and films were successfully prepared by sol - gel method. by differential thermal analysis ( dta ), scanning electron microscopy ( sem ), transmission electron microscopy ( tem ), x - ray diffraction ( xrd ), fluorescent analysis and dielectric constant analysis, the morphology and phase transformation, crystalline properties, fluorescent properties and dielectric properties of the materials were studied

    本研究中採用溶膠凝膠法制備了具有優良結晶性能的稀土離子tb及納米碳管摻雜的鈦酸鉛（ pt ）超細粉末和（ 100 ）取向生長的薄膜，並利用差熱分析、掃描電子顯微鏡、透射電子顯微鏡、 x射線衍射、熒光分析和介電常數等多種分析測試手段對摻雜后的pt薄膜及超細粉末的相結構、光學性能、結晶性能以及介電常數等進行了研究。
  17. This study we acquired the coding region of hcv ns5b gene by pcr of hcv full length genome and construct the recombinant plasmid pegep n3 - ns5b ; with the different concentration of g418 in the culture medium, we think the selection concentration of g418 for hepg2 cell is 800 g / ml ; the recombinant plasmid was transfected into hepg2 cell by lipofectamine2000 cells containing stable transformants were selected by the ability of resistance to g418 and isolated with the limited dilution. the stably transfected cell line expressing ns5b - egfp fusion protein was obtained by the detection under fluorescence microscope and rt - pcr

    本研究首先從hcv基因組中擴增出nssb基因，構建了nssb基因與報告分子egfp （增強型綠色熒光蛋白）基因的融合基因真核表達質粒pegfpn30 ；通過含有不同g418濃度梯度的培養液培養hepgz細胞，確定了篩選用g4181作濃度為800pg ml ；利用脂質體法將該重組質粒轉染hepgz細胞，經過有限稀釋法和g4壓力選擇，應用熒光顯微鏡和rtpcr檢測，獲得可穩定表達nssbegfp融合蛋白的hepgz細胞克隆。
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