片鏈態 的英文怎麼說
中文拼音 [piānliàntài]
片鏈態
英文
stream line regime-
Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method
利用從國外引進的新城疫熱穩定性天然弱毒b _ ( 95 )株接種spf雞胚繁殖病毒,經處理后提取病毒的基因組rna ,參考國內外發表的ndv融合蛋白基因序列,設計一對特異性引物,經反轉錄聚合酶鏈式反應( rt - pcr )擴增出約1700bp大小的特異性片段,將此片段回收純化后,利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中,再轉化大腸桿菌jm109感受態細胞,轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。Its arithmetic just based on hops counting is low efficiency and unilateralism when the network have complex link state
面對現實網路系統中各種復雜的鏈路形態,僅僅基於躍點計數的路由演算法是低效和片面的。The dissertation consists of five chapters : in chapter one, the recent progress in molecular approaches in systematic studies of macroalgae e. g. dna extraction, restriction endonuclease fragment length polymorphisms ( rflps ), random amplified polymorphic dna ( rapd ), gene sequencing, intersimple sequence jepeats ( issr ), amplified fragment length polymorphisms ( aflp ) and single strand. conformation polymorphisms ( sscp ) were reviewed
本論文由五部分組成:在第一部分,綜述了大型海藻dna的提取、限制性片段長度多態性( rflps ) 、隨機擴增多態性dna ( rapd ) 、核酸序列分析、擴增片段長度多態( aflp ) 、單鏈構象多態( sscp )等分子手段在大型海藻系統學研究中應用的一些進展。After electrophorised on 1 % agarose gel, the pcr production was purified with agarose gel dna extraction kit. the segment was ligated with vector pmd18 - t and then was tranformed into the competent cell of dh5 a. a construction mstnd - pmd18t was generated by inserting the sequence of 254bp into pmd18 - t vector and selecting the sense clones. positive clone was identified by three ways : endonuclease digestion, pcr and sequencing. the result showed that the cloned sequence coincides with the designed sequence. this construction was digested with nco i and xho i and ligated the pet28a ( + ) vector digested with the same enzymes using dna ligation kit. the production of ligation reaction was transformed into the competent cell of bl21 ( de3 ). after 12 - 16 hours of culture, several colnes appeared on the plate. some positive clones were selected to extract their plasmid. these plasmids were digested by nco i and xho i and indentified by pcr. a contraction, mstnd - pet28a was generated. the result showed that the cloned sequence coincides with the designed sequence
F _ 1長38bp , r _ 1長36bp ,其它片段均40bp長, f _ 1和r _ 1片段兩端分別加上限制性內切酶nco和xho的識別位點序列。用成對單鏈片段進行延伸反應,然後用其他單鏈片段作為引物,進行pcr擴增,用dna快速純化回收試劑盒回收所得254bppcr產物,與pmd18 - t載體連接、轉化dh _ 5 。受體菌感受態細胞,利用藍白斑遺傳學篩選法篩選陽性克隆,提取其質粒,採用nco和xho雙酶切鑒定,獲得了254bp的片段;用pmd18 - t載體上的特異引物rv - m和m13 - 47進行pcr鑒定,獲得300bp的片段。The microcrystalline was same to the direction of electric field. the microcrystalline orientation of the film that was obtained by melting pvdf congealing in the effect of electric field changed along electric field obviously. comparing with the film congealing without electric field their crystalline plate become thick
發現pvdf纖維在電場作用下微觀結構變化較大,極化后微晶取向發生較大變化,極化以後微晶變化為沿電場方向取向的厚片狀,熔融態的pvdf在電場作用下凝固時變化更明顯,與不加電場直接凝固的pvdf膜相比,在電場作用下幾乎所有的分子鏈都沿電場方向取向所以出現有序的片晶結構,而且結構緻密。This dissertation expands on a new algorithm, which has the function of analyzing edge form with the chain code. by the introduction of the concepts of relative chain code and absolute chain code, this paper proposes a simple and direct algorithm to compute chain code sum ( average chain code )
為了實現骨髓切片圖像細胞的形態特徵,應用鏈碼分析邊界形狀的新方法,引入相對鏈碼與絕對鏈碼概念得到了計算鏈碼和(平均鏈碼)的簡潔演算法。If microalbuminuria reflects changes occurring at the filtration membrane leel, the ealuation of albumin in all three states ( whole molecules, antibody - recognizable molecules, and peptide fragments ) may be required
如果微量白蛋白尿反映的是發生在濾過膜水平的改變,那麼三種狀態的白蛋白(完整分子、抗體可識別的分子、肽鏈碎片)都需要評估。Graphical information is copied between microsoft project and other programs as a static picture only - a snapshot of information that ' s not linked to the document in which it was created
在microsoftproject和其他程序間復制時,圖形信息作為靜態圖片(未鏈接到創建它的文檔的信息圖片)處理。All the subjects were genotyped by pcr - rflp ( polymerase chain reaction - restriction fragment length polymorphism ) at polymorphic sac i site inside the exon 7 of the ahsg gene. this polymorphism involves a nucleotide substitution of c to g at the middle nucleotide of the codon at amino acid position 238 resulting in the replacement of threonine ( acc ) with serine ( agc )
所有的樣本通過聚合酶鏈式反應?限制性片段長度多態性方法( pcr - rflp )對ahsg基因的第7個外顯子內的sac多態性位點進行基因分型,該多態性位點為238號氨基酸密碼子中間的堿基c到g的替換,使蘇氨酸( thr , acc )變為絲氨酸( ser , agc ) 。Areas in microsoft project that can display picture information from another document or program, including static pictures and embedded and linked objects
用來顯示來自其他程序或文檔的圖片信息(包括靜態圖片和嵌入或鏈接對象)的microsoftproject區域。分享友人