瓊脂片法 的英文怎麼說
中文拼音 [qióngzhīpiānfǎ]
瓊脂片法
英文
agar disk method-
The 2nd pair of primers was designed according to the sequence of strain th - 98 collected in genbank. concentration of tp was analyzed after amplification invitro by rt - pcr, purified by low melt agarose and labeled by digoxigenin
根據genbankth - 98株序列設計第2對引物，應用rt - pcr方法體外擴增該片段（命名為tp ） ，瓊脂糖凝膠純化后測定其濃度和純度，進行非放射性地高辛標記。The results also explained that it was feasible that the genomic dna of bacillus subtilis were extracted and gained large dna fragments by low melting - point agarose embedding method
說明以低熔點瓊脂糖包埋法提取枯草桿菌基因組dna ，並獲得較大的dna片段，是完全可行的。Extraction of large - fragment genomic dna in order to gain dna template of pcr amplification ( long pcr amplification and salvage pcr amplification ) which was high purity and large fragment, three methods were used to extract genomic dna of bacillus subtilis, i. e. low melting - point agarose embedding method, sds - proteinase k - phenol chloroform extraction method and bacterial genomic dna extraction kit method. the genomic dna of bacillus subtilis were gained by these methods, and the operated programs of the methods were improved. the results showed that the genomic dna extracted by low melting - point agarose embedding method were obviously biggest than that of another two methods
大片段基因組dna的提取為了獲得用於pcr擴增（長距離pcr擴增和分段pcr擴增）的高純度、大片段（至少為pcr產物長度的4倍）的dna模板，應用三種方法：低熔點瓊脂糖包埋法， sds -蛋白酶k -酚氯仿抽提法和細菌基因組dna提取試劑盒法，分別提取獲得了枯草桿菌基因組dna ，並對3種方法的操作程序進行了不同程度的改進，結果表明：低熔點瓊脂糖包埋法提取的基因組dna片段明顯大於后兩種方法，採用0 . 5瓊脂糖凝膠電泳3h ，仍然跑不出加樣孔。Methods : the qualitative analysis was used by the filter papers, and the quantitative analysis was done by tube method
方法:採用了濾紙擴散片法進行定性試驗，試管二倍稀釋法和瓊脂平板擴散法進行定量試驗研究。分享友人