瓊脂糖電泳 的英文怎麼說
中文拼音 [qióngzhītángdiànyǒng]
瓊脂糖電泳
英文
agarose electrophoresis-
The value of diploid peak before gi was larger in the higher concentration and longer time of arsenic trioxide on floe cytometry
從凋亡細胞中提取dna片段在2瓊脂糖電泳，顯現典型凋亡dna片段142bp 、 174bp梯形帶。( 2 ) the mortality of cp cell in 10 were increasing directly related with the time in the low temperature. after 120d some cp cells died in the way of apoptosis. most nucleus of cells were condensed, and chromosome was marginated beside the nucleus inner membrane, cell sizes were reduced, dna ladder showed in dna gel electrophoresis
（ 2 ） cp細胞系在10低溫下細胞死亡率與時間成正比， 120d的細胞具典型的凋亡現象，即細胞核固縮、染色體邊集在核膜內側；細胞體積變小；瓊脂糖凝膠電泳上顯示特徵性的「梯狀」帶。The result shows that mms can induce dna - damage of yeast cells and the situation of dna - damage aggravated with increase of mms concentration
結果顯示mms能夠引起酵母細胞dna的損傷，並且隨著mms濃度的增加dna損傷程度加重， 0 . 5 %瓊脂糖凝膠電泳及eb染色顯示1 ~ 。Extensive mitochondrial dna polymorphisms were found among lagurus lagurus, mus musculu ， rattus norvegicus and mice. the findings will help us to understand the dispersion and evolution of these animals
通過瓊脂糖凝膠電泳對這些片段進行測定，同時估算出草原兔尾鼠線粒體dna的長度約為16 . 6kb 。To find dnaase in the earthworm the tissue extract of earthworm with different concentration reacted with rna, circular dna, linear dna for an hour at 37, then the producetion was detected by 1 % agrose gel. the tissue extract of earthworm, the tissue protein extract of earthworm and the tissue extract of earthworm without protein reacted with pbv220 - r - inf for an hour at 37, then the producetion was detected by 1 % agrose gel. 2
雙胸蚓組織中dna酶的發現用不同濃度的雙胸蚓組織提取液與rna 、環狀dna及線狀dna在37反應1小時後用1的瓊脂糖凝膠電泳對其反應產物進行觀察；雙胸蚓組織粗提取液、雙胸蚓組織蛋白粗提取液及雙胸蚓組織去蛋白提取液分別與pbv220 - ? inf質粒37反應1小時後用1的瓊脂糖凝膠電泳對其反應產物進行觀察。Extraction of large - fragment genomic dna in order to gain dna template of pcr amplification ( long pcr amplification and salvage pcr amplification ) which was high purity and large fragment, three methods were used to extract genomic dna of bacillus subtilis, i. e. low melting - point agarose embedding method, sds - proteinase k - phenol chloroform extraction method and bacterial genomic dna extraction kit method. the genomic dna of bacillus subtilis were gained by these methods, and the operated programs of the methods were improved. the results showed that the genomic dna extracted by low melting - point agarose embedding method were obviously biggest than that of another two methods
大片段基因組dna的提取為了獲得用於pcr擴增（長距離pcr擴增和分段pcr擴增）的高純度、大片段（至少為pcr產物長度的4倍）的dna模板，應用三種方法：低熔點瓊脂糖包埋法， sds -蛋白酶k -酚氯仿抽提法和細菌基因組dna提取試劑盒法，分別提取獲得了枯草桿菌基因組dna ，並對3種方法的操作程序進行了不同程度的改進，結果表明：低熔點瓊脂糖包埋法提取的基因組dna片段明顯大於后兩種方法，採用0 . 5瓊脂糖凝膠電泳3h ，仍然跑不出加樣孔。The apoptosis induced by extract of russula subnigricans hongo was investigated in little white rat liver and kidney cells by agarose gel electrophoresis. the result showed that agarose gel electrophoresis of dna extracted from poisoned little white rat liver and kidney cells revealed typical 180 ~ 200bp integer - fold " ladder " " bands. apoptosis induced by extract of russula subnigricans hongo was dose - and time - dependentthe result indicated that extract of russula subnigricans hongo could induce apoptosis in little white rat liver and kidney cells
4 .用電泳技術研究亞稀褶黑菇粗毒液誘導小白鼠肝腎細胞凋亡，小白鼠亞稀褶黑菇抽提液中毒后，肝腎細胞. dna經瓊脂糖凝膠電泳出現180一200bp整數倍的ona梯形帶， 19 . 09 / l一28 . 59 / l范圍內，亞稀褶黑菇提取液誘導肝腎細胞凋亡表現出時間和劑量依賴性The inhibition of lower concentrition of tps and egcg is stronger than the inhibition of higher concentrion for cancer cells
處理后的d6細胞， dna瓊脂糖凝膠電泳出現典型的dna梯狀條帶。Pcr products were detected on a 1. 5 % agerose gel, stained by ethidium bromide. the bands containing the amplified fragments were visualized under uv illumination
5經漠化已錠染色的瓊脂糖凝膠電泳后，紫外檢測儀下觀察擴增結果。Recovered the agarose and identified by agarose gelelectrophoresis. the producys of pcr fragments and pcambial303 plasmid ligation were transformed into e. coli ( dh5a ). the result of pcrof positive recombinant and restriction analysis demonstrated that the plant expressing vector of tps is attained
Pcr產物經回收后，經瓊脂糖凝膠電泳鑒定后，與pcambia1303連接並轉化大腸桿菌dh5a ，陽性重組子經pcr和限制性內切酶酶切圖譜分析，表明已獲得海藻糖- 6 -磷酸合成酶基因的植物表達載體。I and hindlll respectively. after the digested products were run via agarose gel electrophoresis and transferred into nylon membrane, the southern blot was carried out using the cdna of rubber tree etrl as probe. the result of the southern blot showed that a hybridization band ( - 3. 0kb ) turned up from the ecor i digested product and another band ( - 4. 8kb ) turned up from the hindi ]
從橡膠樹pr107品系的嫩葉提取基因組dna ，用限制性內切酶ecor 、 hinofll分別酶切，瓊脂糖凝膠電泳分離並轉膜后，用克隆的橡膠樹etri基因的cdna片段作探針進行southern雜交分析，結果表明， ecor酶切在約3 okb處有一條雜交帶出現， hi 。Dna ladder bands with a periodicity of about 200 bp were clearly seen in agarose gel electrophoresis pattern
瓊脂糖凝膠電泳基因組dna可見到約以200bp為間隔的dna梯狀（ ladder ）條帶。Methods : the effects of different neurotrophic factors on the growth and differentiation of neural stem cells were observed by cells counting and immunofluorescence staining. the levels of rara mrna and rxra mrna in differentiated neural stem cells were assayed by rt - pcr. agarose gel electrophoresis and image analysis
方法應用細胞計數和免疫熒光細胞化學法，研究不同神經營養因子對神經幹細胞增殖及分化的影響；應用rt - pcr 、瓊脂糖凝膠電泳和紫外分光圖象分析法檢測神經幹細胞分化過程中rar和rxr mrna表達量的改變。結果1A final period of extension was carried out for 9 min and final holding at 4. the pcr products were resolved after electrophoresis in 2 % agarose gel with ethidium bromide. the pcr products were visualized while the gel was exposed to ultraviolet light
擴增產物在2瓊脂糖凝膠上電泳，用pcrmarker作分子量標準，紫外燈下觀察，最適pcr反應條件為擴增出最強的sry產物帶而無非特異性擴增帶出現時的反應條件。The high purity of genomic dna extracted by tripure isolation reagent was observed. dna agarose gel electrophoresis showed that the genome had a high integrity without degradation. and also, spectrophotometric analysis indicated that the genomic dna had no pollution by protein and rna. 2
培養乳酸乳球菌nizor5 ，收集菌體，用tripurelsolationreagent提取其基因組dna ，瓊脂糖凝膠電泳，紫外顯示儀下可見在點樣孔附近有一條整齊均一的dna帶。Apoptosis was identified by dna electrophoresis and flow cytometry. the results showed the supernatant containing hsblys induced a does and concentration - dependant increase in apoptosis cells
Dna瓊脂糖凝膠電泳和流式細胞儀結果顯示， hsblys表達上清可誘導k562細胞凋亡，且具有劑量依賴效應。A dna band about 1700 bp in length was found in the rt - pcr products of both strains after an agarose - gel electrophoresis, which was consistent with the length of hn registered in genbank
瓊脂糖電泳結果顯示，其大小均約為1700bp ，與genbank登錄的大小一致。其次，將hn基因cdna定向插入pcdna3中。The purified products were cloned into pgem - t - easy vector successfully, the cloned plasmids were transformed into e. coli. tgl. the specific recombinant plasmid was identified by molecular weight, pcr and restriction endonuclease analysis. the results indicated that the resultant construct contained the gene of interest hn at right orientation of the insert
經瓊脂糖電泳檢測，將大小與預計分子量一致的片段純化后連接到pgem - t - easy克隆載體中，再轉化大腸桿菌tgi感受態細胞，得到的轉化子經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。Construction, preparation and quantity of cea dna vaccine : plasmid pgem4 - cea containing full - length of cea cdna was cleaved by ecori, 2. 4kb fragment was recovered, and ligated with ecori - cleaved plasmid pci - neo
Pci n的構建制備和定量： ecori酶切含仍a全基因的質粒pgem 。 cea ，瓊脂糖電泳回收2The purified production was cloned into pmd18 - t vector. the cloned plasmids were transformed into jm109. the specific recombinant plasimid was identified by molecular weight, pcr and restriction endonuclease analysis. the results indicated that the resultant construct contained the gene of ibdv a fragment at right orientation
經瓊脂糖電泳檢測，將大小與預計分子量一致的片段純化后連接到pmd18 - t載體中，再轉化到jm109感受態細胞，得到的轉化子經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆，結果表明，得到的陽性重組子中含有a節段全長基因，插入到載體中的方向正確。分享友人