甲基化酶 的英文怎麼說
中文拼音 [jiǎjīhuà]
甲基化酶
英文
cyp51-
This complex reaction is catalyzed by anthranilate synthetase.
這個復合反應是被鄰氨基苯甲酸合成酶催化的。Determination of the catalytic structures of methyl parathion hydrolase
甲基對硫磷水解酶參與催化相關結構的研究When both genes were co - expressed in e. coli, the activity of ppsa varied from 2. 1 - 9. 1 fold comparing to control, but the activity of tkta was relatively stable ( 3. 9 - 4. 5 fold ). whatever the two genes were expressed respectively or cooperatively, both could promote the production of dahp, the first intermediate of the common aromatic pathway, but co - expression was more effective on forming dahp and screened ppt - and ptp - as more effective. the results demonstrate that co - expression of ppsa and tkta can improve the production of dahp, and what ' s more, when multigenes co - expressed, the recombinant which has coordinated enzymes activity is optimum
莽草酸途徑的最優化和整體調控基因csra的敲除正是上述改變的分子基礎,同時也為三種芳香族氨基酸的基因工程菌的構建打下了基礎; 7 .在國內外首次實現了共同途徑限制性底物關鍵酶ppsa刁無『及arog與分支途徑關鍵酶基因phea的串聯高效表達,所構建的重組質粒ptga ,其ppsa 、 tkta 、 arog 、 cm和pd的酶活分別比對照提高了3 、 2 、 2 , 5 、 4 、 2 . 3倍,且其酶活比較協調一致; 8 .將ptga導入到篩選的基因敲除和基因替換菌株大腸桿菌31884 c甲b中,搖瓶發酵證實比以往所構建的基因工程菌株具有較高的phe產量和糖轉化率率,分別為0 . 448 %和22 . 4 % 。The transfermants with highest resistance to g418 ( 4 g / l in ypd ) were screened to study their expression level in 150 ml shaking flask. having been induced with methanol for 36 hours, the target enzyme could be examined in the supernatant by measuring amidolytic activity
首次將大連蛇島賅蛇毒類凝血酶成熟基回克隆到表達載體ppicgk中,經電激轉化至畢氏酵母菌株gs15中,再經甲醇誘導,在150ml搖瓶畔1獲得細胞外分泌表達產物。This paper describes several latest industrial microbial technologies in detail, which are the synthesis of the chiral diols by epoxide hydrolase from microbie, cofactors regeneration for redox with fdh, production of nano / micro wire by the phage display, metabolic network rebuilding for conventional fermentation and the application of the organic solvent tolerance and the metagenomics technology
本文綜述了幾項最新的工業微生物技術,主要包括:微生物環氧化水解酶催化合成手性二醇、微生物甲酸脫氫酶用於再生氧化還原反應的輔因子、通過噬菌體展示技術得到納米級金屬絲、代謝網路改造和重建用於傳統發酵生產以及有機溶劑耐受菌和宏基因組技術的應用。The vasomotion was induced by noradrenaline na in vivo, then the changes of vasomotion after injecting si and ngmonomethyllarginine lnmma, a no synthase inhibitor were measured respectively on a tv monitor using a tv camera mounted on the microscope, and the influence of lnmma on effect of si was also observed
在活體情況下運用去甲腎上腺素noradrenaline , na血管運動誘導法誘導出血管運動,分別觀察靜脈注射g單甲基精氨酸gmonomethyllarginine , lnmma si后血管運動的變化及應用一氧化氮合酶抑制劑lnmma對si作用的影響,攝錄結果於監視器上用暫停功能測量。Immobilization of penicillin g amidase on beaded glycidyl methacrylate copolymer support
青霉素酰化酶在甲基丙烯酸縮水甘油酯共聚物上的固定化Based on the principle of enzyme inhibition, using the immobilized chicken liver esterase and a flow injection analysis ( fia ) enzyme biosensor ( developed by ourselves ), this paper investigated the inhibiting power of several insecticides to the enzyme, and selected methamidophos as the standard pesticide for the quantitive determination
摘要本文基於酶抑制原理,採用固定化雞肝酯酶和自行研製的流動注射式酶生物傳感器,研究了不同農藥對酶的抑製程度,確定了甲胺磷作為農藥檢測的定量標準。In other studies of mutant ( knockout ) mice that fail to express the neuronal isoform of nitric oxide synthase ( nos ), smaller infarcts develop and the mice have less severe neurologic deficits than normal mice ( 61 )
其他一些研究變異(被擊昏的)老鼠的試驗沒有能夠表達一氧化氮合酶( nos )的神經元對碘氧基苯甲醚,但是出現的梗死梗小,而且與正常的老鼠相比,老鼠的神經學缺陷也不那麼嚴重( 61 ) 。In bioconversion of a d - amino acid and its derivative, the substrate, 5 ' - monosubstituted hydantoin is firstly transferred into an intermediate, d - carbamyl amino acid by d - hydantoinase, and in turn into an optically active d - amino acid by either d - carbamoylase or chemical, nano2. nowadays, the bioconversion of d - amino acids has been industrialized by enzymes, so called one - step process
D -型氨基酸及其衍生物的生物轉化,經歷兩個反應歷程,首先是在海因酶的作用下,催化5 -單替代海因形成氨甲酰類氨基酸中間物,後者再在酶或化學的作用下,生成相應的光學活性氨基酸或其衍生物。The l - n - carbamoylase of arthrobacter bt801, coded by the hyuc gene, is the rate - limiting and the only stereoselective enzyme. hydantoin hydrolase gene ( hyuh ) and n - carbamoylase gene ( hyu c ) were amplified from the plasmid of puc18 - 169 by pcr and, at the same time, activity expression was obtained in e. coli and pichia pastor is gs115 respectively
節桿菌bt801的乙內酰脲水解酶催化乙內酰脲類化合物水解開環, n -氨甲酰基氨基酸水解酶是該菌乙內酰脲酶系中惟一具有立體選擇性的酶,也是整個反應體系的限速酶。A. niger m - l which was screened in our laboratory produced a strongly a - transglucosidase. in this paper, studies on the fermentation conditions, purification and characterization of a - transglucosidase and its necessary groups was carried out in this dissertation. the main reports were as following : the fermentation conditions in shaking flasks were investigated by the method of single - factor analysis, the suitable main medium was achieved : which contained 4 % a, 2 % b and 1 % g ; the a. niger m - l was inoculated into 100ml medium in flask, shaking in 33 c at 140r / min for four days, with initial ph6. 5 and 6 % inocula volume ; adding 0. 1 mmol / l methyl a - d - glucopyranoside had inductive effect on enzyme formation, the a - transglucosidase activity amounted to 296. 05u / ml
本研究以黑麴黴m - 1為出發菌株,對其-葡萄糖轉苷酶的產酶影響因素、純化、酶學性質以及必需基團進行系統的研究,結果如下:通過對影響黑麴黴m - 1產-葡萄糖轉苷酶的單因素分析,得液態發酵生產-葡萄糖轉苷酶的最適產酶條件為: 4 a , 2 b和1 g ;在33 ,起始ph值為6 . 5 ,轉速為140r min ,接種量為6 ,裝液量100ml條件下,發酵4 . 0d ,酶活力達296 . 05u ml ,添加0 . 1mmol l的酶作用底物甲基- - d -葡萄糖苷對產酶的誘導作用最大。The objective of this research is to transform the cloned phytase gene into pichia pastoris in order to obtain high - yield phytase - producing strain and to optimize the engineered yeast media recipes for the scale - up production of phytase. main results of this research are as follow : 1, xba i - linized recombinant plasmid ppic9k - phya was transformed into pichia pastoris by gene pulser. 98 positive transformants showing measurable phytase activities were screened on md plates and ypd plates containing g418. they all grew quickly on both md plates and mm plates, which proved their phenotypes of methanol utilization were mut +
主要實驗結查如下:西南農業大學碩士學位論文1 、用乃ai酶切攜帶植酸酶基因表達片段的重組質粒ppicgk夕句) a ,回收dna ,用genepulser電擊轉化畢赤酵母,塗布md平板,又用含不同濃度g418的ypd平板進行抗性篩選,得到98個可檢測到植酸酶活力的陽性轉化子,它們在md 、 mm平板上均表現快速生長,說明其甲醇利用表型是mut干。Nitro - l - arginine methyl ester inhibits the upregulated expression of nitric oxide synthase nmda receptor in the morphine analgesia tolerance rats
一氧化氮合成酶n -甲基- d -天冬氨酸受體表達上調In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals
首先,用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上,並轉化e colidh5a感受態細胞中,經pcr 、酶切以及測序證明得到了完整的2c3abc基因,並與國內外參考序列進行比較分析。然後,將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ,以zeocin ~ ( tm )抗性篩選陽性克隆,大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞,通過zeocin ~ ( tm )抗生素梯度濃度篩選,獲得重組酵母用0 . 5甲醇誘導表達,通過sds - page電泳、 westernblotting分析,結果表明, 2c3abc基因在畢赤酵母中成功表達,其表達產物為一95ku的融合蛋白,並能被口蹄疫病毒陽性血清識別。As far as the enzymatic activity is concerned, on the one hand, strain yz - ii6 has higher d - hydantoinase activity and lower d - carbamoylase activity so as not to be suitable for one - step bioconversion of d - amino acids, on the other hand, the higher hydantoinase activity, engineered strain in particular, may be convenient to be as a biocatalyst to produce n - carbamyl d - amino acids which hard to find in the markets
Yz - 6菌具有較高的海因酶活性,但n -氨甲酰基d -氨基酸酰氨水解酶活性很低,仍不適合用於d -型氨基酸生產工藝中的一步法轉化。另一方面,含海因酶基因的人工菌株不但酶活性高,也排除了天然菌株轉化生產d -型氨基酸過程中的一些副產物。The positive transformants with the integrates mn - sod gene was identified by zeocin - resistance, pcr screening and expression in p. pastoris. the recombinant mn - sod protein was successfully expressed in pichia pastoris based on the evidences that a relative molecular weight about 23kd appeard in sds - page, the obvious activity of sod existed in native - page and enzymatic activity test, and mn - sod activity was specific base on the inhibition with the mixture of chloroform - enthanol ( 3 : 5 / v : v ) and potassium cyanide. two secreted plasmids ppiczaa - sodm18 and ppiczaa - sodc were constructed and after there linearization were transferred into chromosome of pichia pastoris gs115 by electroporation
Pcr鑒定及mut表型分析進一步說明,目標基因已經重組到宿主菌基因組染色體上; 0 . 5甲醇誘導表達后, sds - page結果顯示,表達的蛋白相對分子量約為23kd ,活性電泳出現明顯活性條帶;酶活性測定顯示,重組菌株sod活性比對照提高5倍左右;氯仿-乙醇( 3 : 5 v : v )和kcn ( 5mmol l )抑制反應進一步證明,所表達的sod為錳超氧化物歧化酶。分享友人