疏酸基 的英文怎麼說
中文拼音 [shūsuānjī]
疏酸基
英文
acidophobe-
Full - length or truncated cdna was subcloned into prokaryotic expression vector pet30a and expression induced in e. coli bl21 ( de3 ). no squalene synthase polypeptide of expected molecular mass was observed in e. coli containing the putative full - length squalene synthase cdna, however, overexpression in e. coli was achieved by truncating 30 hydrophobic amino acids at the carboxy terminus
但在含有全長的鯊烯合酶cdna的大腸桿菌中並沒有觀察到預期大小的鯊烯合酶表達,而c末端截短30個疏水氨基酸的鯊烯合酶可在大腸桿菌中過量表達。To investigate the secondary structure of this gene, we found it has a transmembrane region near the n - terminus, followed by a proline - rich conserved region, and it has a conserved haemachrome binding region about 60 aa away from carboxyl terminus
分析氨基酸序列的二級結構發現基因n -端具有跨膜的疏水序列,其後具有富含脯氨酸保守區,在距離羧基端60個氨基酸處具有高度保守的血紅素結合域。The pocket accommodating the aromatic ring of phenylalanine is formed predominantly by hydrophobic side - chains, including those of ile10, ile13, prol50, leul75, leul79, phe209, ser211 and val221 ; whilst the amino group and carboxylate group of phenylalanine is coordinated by asp6, asp7, gln151 and ser180. the n - terminal region is found to be important to the function of arog. point mutation reveals that residue substitution of ilelo to ala10 leads to desensitization in the presence of 1 mm phenylalanine, simultaneously, exhibits a dramatic decrease in enzymatic activity
綜合前人的研究和本研究工作的實驗數據,得出以下結論: 1 )在arog的反饋抑制位點供苯丙氨酸結合的疏水口袋由asp6 、 asp7 、 ile10 、 ile13 、 pro150 、 gln151 、 leu175 、 leu179 、 ser180 、 phe209 、 ser211和val221等12個氨基酸殘基構成; 2 )苯丙氨酸結合的疏水口袋分為兩個區域,與苯丙氨酸的苯環發生疏水作用的區域由ile10 、 ile13 、 pro150 、 leu175 、 leu179 、 phe209和val221的側鏈構成,而asp6 、 asp7 、 gln151和ser180的側鏈構成了與苯丙氨酸的氨基和羧基相互作用的區域。Huangyal4 was complete nucleotide sequence of 1 854 bp with a nucleotide orf ( 1575 bp ), which encoded a protein consisting of 524 aa with molecular weight of 62. 2 kda and pi of 8. 96. strongly basic ( + ) amino acids, strongly acidic ( - ) amino acids, hydrophobic amino acids and polar amino acids of the protein were 13. 74 %, 11. 64 %, 36. 45 % and 22. 70 % respectively, and predicted secondary structure of the protein revealed many conserved domains such as n - glycosylation site, protein kinase c phosphorylation site, casein kinase ii phosphorylation site, n - myristoylation site, camp - and cgmp - dependent protein kinase phosphorylation site, tyrosine kinase phosphorylation site and a cytochrome p450 cysteine heme - iron ligand signature which was typical of cytochrome p450. a - helix and b - sheet of the protein is 47. 7 %, 45. 0 % respectively
Huangya14 )為材料分離克隆到一個細胞色素p450基因,命名為bccyp86mf5 , cdna全長1854bp ,含1575bp的完整開放閱讀框,編碼524個氨基酸,其編碼蛋白質的分子量為61 . 2kda 、等電點為8 . 96 ;堿性氨基酸、酸性氨基酸、疏水氨基酸和極性氨基酸分別占總氨基酸的13 . 74 、 11 . 64 、 36 . 45和22 . 70 ;二級結構預測包括n -糖基化位點、依賴于camp和cgmp的蛋白激酶磷酸化位點、蛋白激酶c磷酸化位點、酪蛋白激酶磷酸化位點、酪氨基酸激酶磷酸化位點、 n -豆蔻酰化位點和細胞色素p450的典型區域,半胱氨酸亞鐵血紅素配體信號區等, -螺旋和-折疊分別佔47 . 7 、 45 . 0 ;與bccyp86mf1基因的氨基酸序列同源性達到95 . 2 ,與擬南芥cyp86c4的達到85 . 9 。One of them was identified as the beth gene of halobacillus sp. d8. a hydrophobicity plot of beth revealed an alteration of hydrophobic and hydrophilic segments that was characteristic for integral membrane protein, suggesting the presence of 12 transmembrane - spanning segments and belonged to bcct family
將重組質粒測序,插入片段大小為4kb左右,通過blast比較,結果顯示含有三個orf框,其中一個為甘氨酸甜菜堿轉運蛋白,對其氨基酸組成進行疏水性分析,推測為含有12個跨膜域的跨膜蛋白,屬于bcct家族。The deduced amino acid sequence of rbcl and rbcs includes 13. 14 % acidic amino acid and 14. 51 % aliphatic amino acid, the hydrophobic amino acid is 42. 16 %
14的酸性氨基酸, 14 51脂肪族氨基酸,疏水性氨基酸的比例為42 16 。Open reading frames ( orf ) were found in some novel sequences from the library, after that, putative amino acid sequences were obtained, and hydrophobicity profile were analysed
我們還對部分序列做開放閱讀框分析,利用推定的氨基酸序列進行疏水性分析,為研究蛋白的功能和細胞內定位提供線索。Thus, the success of nscs therapy is determined by the induced differentiation and the effect of micro - enviroment on the survival, proliferation and differentiation of it. the isolation and culture of nscs in vitro successfully is the presupposition for its research. to avoid the influence of unknown factors exiting in serum on nscs " differentiation, serum - free medium with egf and bfgf as mitogens to stimulate proliferation and inhibit differentiation in vitro was introduced through a long period of search
澱粉樣蛋白( amyloidpeptidy , a )是澱粉樣蛋白前體蛋白( amyloidprecusorprotein , app )異常代謝產生的含有39 ? 43個氨基酸殘基的疏水肽,可聚合成不溶的纖維形式或不定型顆粒狀結構,導致各種毒性反應,在阿爾茨海默病( alzheimer ' sdisease , ad )發病中起著至關重要的作用。Quantify the relevance hydrophobicity in protein structure : the general expectation is that hydrophobic aminoacids are in the core of proteins, while polar aminoacids are on the surface
量化蛋白質結構的參比疏水性:一般認為,疏水性氨基酸在蛋白質的核中,而極性氨基酸在表面。Many scholars think they have not important physical roles because of their high working concentration. they have the same n - terminal amino acid and c - terminal amino acid, namely asparine and cysteine respectively. they form two largely the same hydrophobic domains
Bsp - a1 a2和a3的n -端都是天冬氨酸, c -端為半胱氨酸,並都含有兩對二硫鍵,由此形成了兩個基本相同的疏水結構區。Ag + and fe2 + caused the fluorescence of trp residues in g6pd quenched ; mg2 + and edta made fluorescence intensity of g6pd increased, this indicates that they caused trp residues wrapped and came to the inner core and located in the hydrophobic area ; while zn2 + or mn2 + made fluorescence intensity of g6pd decreased, this indicates that they made the conformation of g6pd relaxed and chromophores exposed to polarity environments. in native condition and in the far circular dichroic ( cd ) region, g6pd exhibited two characteristic negative band centered at 208nm and 222nm respectively, thus it is estimated to contain about 41. 2 % a - helix, 20. 6 % - pleated sheet and 38. 2 % random coil and turn
Ag ~ +和fe ~ ( 2 + )引起色氨酸( trp )殘基的熒光淬滅; mg ~ ( 2 + )和edta均使g6pd的熒光強度增強,說明它們使trp殘基重新包裹在分子內部而處于疏水的微環境中; zn ~ ( 2 + )和mn ~ ( 2 + )均使g6pd的熒光強度變小,說明它們使酶分子構象變得疏鬆,原來處在分子內部的發色團暴露在極性環境中。Asn124 - ser125 - thr126. similarly, the full length nucleotide sequence of the cdna of gussurobin, a thrombin - like enzyme from gloydius ussuriensis. was obtained
由於天然形式存在的兩種酶活性差別較大,說明這三個氨基酸疏水性的改變對酶的高級結構產生了影響。Hydrophobicity analysis predicted a 36 - residue hydrophobic signal peptide for secretion in the n - terminus, and no transmembrane region was present, suggesting it might be a type of secretory protein. some generic and atipical n - glycosylation sites were present in clsp, suggesting that the protein might represent a glycoprotein. the clsp protein contained one cub ( clr / cls, an embryonic sea urchin protein uegf, and bone morphogenetic protein i ) domain from 39th to 164th ammo acids, which is known to be involved in protein - protein or protein - substrate interaction, and a trypsin - like serine protease domain positioned from 244th to 483rd amino acids
Clsp分子具有補體樣絲氨酸蛋白酶的多種結構特徵,包括36個氨基酸組成的疏水信號膚,一個cub ( clr / cls , anembryonicseaurehinproteinuegf , andbonemorphogeneticproteinl )結構域和一個胰酶樣絲氨酸蛋白酶結構域( t汀psin一likeserineproteasedomain )和幾個保守的糖基化位點等,沒有發現有跨膜區的存在。The study of in - situ construction of cytocompatible surface on pdl - la matrix via amphiphile - amino acid ( rgd ) hybrid self - segregation - the amphiphilic diblock copolymer, poly ( dl - lactide ) - poly ( ethylene oxide ) ( pla - peo ) copolymer, containing hydrophobic pla block and hydrophilic peo block was synthesized via coupling method in this dissertation. cell - adhesion - promoting amino acids and integrin receptor peptide rgd were then immobilized at the end of peo chain of pla - peo copolymer via hydroxyl group activation technique. the solvent blending and casting method was then used to obtain the amphiphile modified pdl - la membranes
兩親共聚物-氨基酸( rgd )雜化體原位自修飾構建聚乳酸細胞相容性表面的研究一本論文首先設計併合成了一類含疏水聚乳酸( pla )鏈段和親水聚氧乙烯( peo )鏈段的兩親嵌段共聚物材料( pla - peo ) ,利用peo鏈端的活性官能團羥基固定了促細胞粘附的氨基酸及整合素配體多肽片段rgd 。The fluorescence spectrum ( fls ) of lra excited at 280nm and 295nm showed a maximum peak at 338nm. the characteristic peak of tyr did not exist, and it showed that the fluorescence energy of tyr was transformed to trp and strength the fluorescence of trp. when lra was excited at 295nm, the fls showed a maximum peak at 338nm, the max of fluorescence emission spectrum blue - shifted more than 10nm compared with the max of free tyr ( 348nm )
Lra的熒光光譜研究表明在激發光波長為280nm時,其最大熒光發射峰在338nm處,熒光光譜未見有酪氨酸( tyr )殘基的發射峰,表明tyr殘基的熒光基本上通過能量轉移到trp上,使熒光強度增強,在激發光譜為295nm時,其最大熒光發射峰338nm ,比游離trp的最大熒光發射峰( 348lun )藍移了近10nln ,說明trp周圍的極性較弱,處于疏水的微環境。The gene was 1668bp in length, encoding the f protein composed of 553 amino acids. sequence analysis and its secondary structure prediction showed that the f protein had three major hydrophobic regions consisting of about 25 amino acids, six potential glycosylation sites and thirteen cysteines. a sequence region of basic amino acids, rrqrrf, was found at the f, - f2 cleavage site, indicating that f4ge9 was a typical virulent strain
序列分析和二級結構預測表明, f _ ( 48 ) e _ ( 9 )株f蛋白含有3個分別由25個氨基酸組成的疏水區,存在6個潛在的糖基化位點, 13個半胱氨酸殘基,裂解位點區域的氨基酸序列為rrqrrf ,說明f _ ( 48 ) e _ 9株是一株典型的強毒株。Based on the " self - migrating and surface - segregation " behavior, the amphiphile segregation surface was therefore constructed on hydrophobic pdl - la matrix, which containing the structure of peo spacer combining cell adhesion ligands to mimic the extracellular matrix ( ecm )
基於兩親共聚物在疏水高聚物材料內的自遷移和表面富集行為,在聚乳酸材料上構建了以peo橋聯氨基酸或整合素配體多肽片段的類細胞外基質表面。Signal recognition particle ( srp ) - - a particle composed of proteins and 7sl rna that binds to signal sequences and targets polypeptide chains to the endoplasmic reticulum
訊息(指揮)序列- -蛋白質末端的一段疏水性氨基酸,在細菌中引導蛋白質分泌或在真核細胞中將蛋白質併入內質網。Signal ( leaser ) sequence a hydrophobic sequence at the amino terminus of a polypeptide chain that targets it for secretion in bacterial or incorporation into the endoplasmic reticulum in eukaryotic cells
訊息(指揮)序列蛋白質末端的一段疏水性氨基酸,在細菌中引導蛋白質分泌或在真核細胞中將蛋白質併入內質網。In order to investigate the effects of ph, temperature, naf and bivalent cations on the conformation of the phosphatase in solution, we monitored the difference of intrinsic fluorescence of the phosphatase and compared changes of the enzyme activity under those conditions. the tertiary structure loosed and the intensity of fluorescence decreased below ph 6. 0. the intensity of fluorescence was lowest at ph 5. 0 and the tertiary structure was reconstructed as the solution ph was increased
其中在ph5 . 0及以下時,蛋白質的三級結構變得鬆散,熒光強度下降, ph5 . 0時尤為顯著,而當溶液ph高於5 . 0時(酶的最適ph ) ,樣品的熒光發射強度明顯增加,表明酶蛋白受溶液酸堿度的影響,構象發生部分變化,部分trp殘基向疏水環境移動,其三級結構得到恢復。分享友人