疏酸的 的英文怎麼說
中文拼音 [shūsuānde]
疏酸的
英文
acidophobous-
Waxes form a continuous lipid membrance covering the epidermal cells of all aerial plant organs. it mainly comprised of lony - chain aliphatic compounds derived from very long chain fatty acids that are enlongedsfrom c16 - or c18 - long fatty acids in er by many fatty acid elongation ( fae ) complexs
陸生植物的氣生器官的表皮覆蓋著一層由蠟質形成的脂膜,它主要由長鏈疏水物質組成的,這些物質是特長鏈脂肪酸的衍生物。Iron or steel placed in copper sulfate also receives a loose immersion deposit.
鐵或鋼放入硫酸銅中,也會生成疏鬆的浸鍍層。Experimental research of shugan hewei pill on inhibiting the secretion of gastric hydrochloric acid and pepsin of rats with gastric ulcer
疏肝和胃丸抑制胃潰瘍大鼠胃酸和胃蛋白酶分泌作用的實驗研究The nuclei are ooid or round with finely reticular chromatin and rare punctate nucleoli. the cytoplasm is pale eosinophilic or clear
細胞核呈圓形或卵圓形,染色質為精細的網路狀,稀疏的斑點狀核仁。胞漿蒼白色或透明,嗜酸性染色。All ha's showed a loose spongy structure with a large number of internal spaces.
所有腐植酸都有疏鬆的海綿結構,同時有大量的內部空間。Full - length or truncated cdna was subcloned into prokaryotic expression vector pet30a and expression induced in e. coli bl21 ( de3 ). no squalene synthase polypeptide of expected molecular mass was observed in e. coli containing the putative full - length squalene synthase cdna, however, overexpression in e. coli was achieved by truncating 30 hydrophobic amino acids at the carboxy terminus
但在含有全長的鯊烯合酶cdna的大腸桿菌中並沒有觀察到預期大小的鯊烯合酶表達,而c末端截短30個疏水氨基酸的鯊烯合酶可在大腸桿菌中過量表達。Then enzyme was purified with a deae - cellulose ( 5. 5x50cm ) column, a toyopearl hw - 65 ( 5. 5 x 50cm ) column and a sephadex g - 200 ( 5. 5 x 80cm ) column. finally, the enzyme was purified for 10 folds with the recovery of 17. 4 %. page showed a single band for the purified creatinase
3 、肌酸水解酶的提純酶在硫酸銨飽和度為40 80之間完全沉澱,先後經過deae - cellulose離子層析柱、 toyopearlhw - 65疏水層析柱、 sephadexg - 200分子篩層析柱層析,最終使酶提純10倍,最終得率為17 . 4 。To investigate the secondary structure of this gene, we found it has a transmembrane region near the n - terminus, followed by a proline - rich conserved region, and it has a conserved haemachrome binding region about 60 aa away from carboxyl terminus
分析氨基酸序列的二級結構發現基因n -端具有跨膜的疏水序列,其後具有富含脯氨酸保守區,在距離羧基端60個氨基酸處具有高度保守的血紅素結合域。In the beginning, the children were shy, but their shyness soon melted in the warm love of the initiates. some held onto the initiates shoulders for a prolonged time, not wanting to let go. such a scene made us sad as we realized how desperately these orphans are in need of love
剛開始顯得有些生疏的小孩們,很快的便能和同修融合在一起,有些更緊緊的依偎在同修的肩上,久久而不能釋手,此時此景,多令人感觸辛酸,他們是那麼迫切的需要人們的關懷,願師父聖愛加持他們的心靈!Effect of shengu capsule on rat osteoporosis model induced by vitamin a acid
腎骨膠囊對維甲酸所致大鼠骨質疏鬆的影響Under these conditions the fibrinolytic activity of the supernatant can reach 820 urokinase units per milliliter broth. ba - dfe was purified from the supernatant of b. amyloiquefaciens dc - 4 culture broth by ammonium sulfate precipitation, ion - exchange chromatography on cm - and deae - sepharose fast flow, hydrophobic interaction chromatography on phenyl sepharose 6 fast flow and gel filtration on sephadex g - 50. the purified enzyme displayed thermophilic, hydrophilic and strong fibrinolytic activity
通過硫酸銨分級沉澱、 cm - sepharosefastflow和deae - sepharosefastflow離子交換層析、 phenylsepharose6fastflow疏水層析和sephadexg - 50凝膠過濾等方法,從解澱粉芽孢桿菌dc - 4的發酵液中分離純化出電泳純的ba - dfe 。The pocket accommodating the aromatic ring of phenylalanine is formed predominantly by hydrophobic side - chains, including those of ile10, ile13, prol50, leul75, leul79, phe209, ser211 and val221 ; whilst the amino group and carboxylate group of phenylalanine is coordinated by asp6, asp7, gln151 and ser180. the n - terminal region is found to be important to the function of arog. point mutation reveals that residue substitution of ilelo to ala10 leads to desensitization in the presence of 1 mm phenylalanine, simultaneously, exhibits a dramatic decrease in enzymatic activity
綜合前人的研究和本研究工作的實驗數據,得出以下結論: 1 )在arog的反饋抑制位點供苯丙氨酸結合的疏水口袋由asp6 、 asp7 、 ile10 、 ile13 、 pro150 、 gln151 、 leu175 、 leu179 、 ser180 、 phe209 、 ser211和val221等12個氨基酸殘基構成; 2 )苯丙氨酸結合的疏水口袋分為兩個區域,與苯丙氨酸的苯環發生疏水作用的區域由ile10 、 ile13 、 pro150 、 leu175 、 leu179 、 phe209和val221的側鏈構成,而asp6 、 asp7 、 gln151和ser180的側鏈構成了與苯丙氨酸的氨基和羧基相互作用的區域。Huangyal4 was complete nucleotide sequence of 1 854 bp with a nucleotide orf ( 1575 bp ), which encoded a protein consisting of 524 aa with molecular weight of 62. 2 kda and pi of 8. 96. strongly basic ( + ) amino acids, strongly acidic ( - ) amino acids, hydrophobic amino acids and polar amino acids of the protein were 13. 74 %, 11. 64 %, 36. 45 % and 22. 70 % respectively, and predicted secondary structure of the protein revealed many conserved domains such as n - glycosylation site, protein kinase c phosphorylation site, casein kinase ii phosphorylation site, n - myristoylation site, camp - and cgmp - dependent protein kinase phosphorylation site, tyrosine kinase phosphorylation site and a cytochrome p450 cysteine heme - iron ligand signature which was typical of cytochrome p450. a - helix and b - sheet of the protein is 47. 7 %, 45. 0 % respectively
Huangya14 )為材料分離克隆到一個細胞色素p450基因,命名為bccyp86mf5 , cdna全長1854bp ,含1575bp的完整開放閱讀框,編碼524個氨基酸,其編碼蛋白質的分子量為61 . 2kda 、等電點為8 . 96 ;堿性氨基酸、酸性氨基酸、疏水氨基酸和極性氨基酸分別占總氨基酸的13 . 74 、 11 . 64 、 36 . 45和22 . 70 ;二級結構預測包括n -糖基化位點、依賴于camp和cgmp的蛋白激酶磷酸化位點、蛋白激酶c磷酸化位點、酪蛋白激酶磷酸化位點、酪氨基酸激酶磷酸化位點、 n -豆蔻酰化位點和細胞色素p450的典型區域,半胱氨酸亞鐵血紅素配體信號區等, -螺旋和-折疊分別佔47 . 7 、 45 . 0 ;與bccyp86mf1基因的氨基酸序列同源性達到95 . 2 ,與擬南芥cyp86c4的達到85 . 9 。The deduced amino acid sequence of rbcl and rbcs includes 13. 14 % acidic amino acid and 14. 51 % aliphatic amino acid, the hydrophobic amino acid is 42. 16 %
14的酸性氨基酸, 14 51脂肪族氨基酸,疏水性氨基酸的比例為42 16 。Ag + and fe2 + caused the fluorescence of trp residues in g6pd quenched ; mg2 + and edta made fluorescence intensity of g6pd increased, this indicates that they caused trp residues wrapped and came to the inner core and located in the hydrophobic area ; while zn2 + or mn2 + made fluorescence intensity of g6pd decreased, this indicates that they made the conformation of g6pd relaxed and chromophores exposed to polarity environments. in native condition and in the far circular dichroic ( cd ) region, g6pd exhibited two characteristic negative band centered at 208nm and 222nm respectively, thus it is estimated to contain about 41. 2 % a - helix, 20. 6 % - pleated sheet and 38. 2 % random coil and turn
Ag ~ +和fe ~ ( 2 + )引起色氨酸( trp )殘基的熒光淬滅; mg ~ ( 2 + )和edta均使g6pd的熒光強度增強,說明它們使trp殘基重新包裹在分子內部而處于疏水的微環境中; zn ~ ( 2 + )和mn ~ ( 2 + )均使g6pd的熒光強度變小,說明它們使酶分子構象變得疏鬆,原來處在分子內部的發色團暴露在極性環境中。The fluorescence spectrum ( fls ) of lra excited at 280nm and 295nm showed a maximum peak at 338nm. the characteristic peak of tyr did not exist, and it showed that the fluorescence energy of tyr was transformed to trp and strength the fluorescence of trp. when lra was excited at 295nm, the fls showed a maximum peak at 338nm, the max of fluorescence emission spectrum blue - shifted more than 10nm compared with the max of free tyr ( 348nm )
Lra的熒光光譜研究表明在激發光波長為280nm時,其最大熒光發射峰在338nm處,熒光光譜未見有酪氨酸( tyr )殘基的發射峰,表明tyr殘基的熒光基本上通過能量轉移到trp上,使熒光強度增強,在激發光譜為295nm時,其最大熒光發射峰338nm ,比游離trp的最大熒光發射峰( 348lun )藍移了近10nln ,說明trp周圍的極性較弱,處于疏水的微環境。A newly made amino acid chain folds into a distinctive shape depending on the positioning of hydrophobic amino acids, which like to cluster together away from the cell ' s watery cytoplasm, leaving hydrophiles to form the protein ' s surface
剛合成好的胺基酸長鏈,會根據疏水性胺基酸的分佈位置,摺疊成蛋白質的獨特形狀:疏水性胺基酸彼此向內聚攏,避免接觸到富含水的細胞質環境;親水性胺基酸則向外翻出,構成蛋白質的表面。The nucleoside identity of the genomic sequence of strain f48e9 to that of la sota, v4, b1, zj1 and clone 30 strain was found to be 87. 3 %, 88. 8 %, 87. 3 %, 85. 4 % and 87. 7 %. on the amino acid sequence level, an identity was 90. 8 - 99. 6 % ( np ), 79. 5 - 99. 5 % ( p ). 89 - 99. 7 % ( m ), 87. 7 - 99. 8 % ( f ), 88. 6 - 99. 5 % ( hn ) and 92. 2 - 99. 6 % ( l ) for this six strain
此外, f48e9株l基因除了編碼一個含有2204個氨基酸多肽外,還可編碼一個52個氨基酸的疏水多肽,而ndv雞源v4 ( hb92 )株和鵝源分離株zj1的l基因不編碼這個小蛋白。These results showed these proteins have a high degree of similarity ; they are basic and cysteine - rich proteins with a signal peptide and a common pattern of eight cysteines that engaged in four disulphide bridges holding together four a helices and stabilizing the structural fold. a hydrophobic central cavity in which can occupied by lipids is found between the four helices. however, it has been difficult to draw any conclusions about the in vivo activity of nsltps from their lipid binding properties because it is unknown which ligands, if any, are bound to nsltps in vivo
不同物種的非特異性轉移蛋白具有很高同源性,它們是堿性的富含半胱氨酸的蛋白質,在n端有一個信號序列, 8個保守的半胱氨酸殘基能形成4個二疏鍵以維持蛋白的空間結構,推測在其空間結構的中心形成了一個能容納脂類物質的洞穴,但在體內還不知道nsltp的配體包括哪些物質,對于nsltp能否在體內能夠結合併轉運脂類還沒有明確的定論。After enzyme restriction and sequencing analysis, the nucleotide data had been further analyzed by antheprot 5. 0 and clutalw softwares. the analysis results showed that the cloned dna fragment had a longest open reading frame ( orf ) of 1035nt, it predicted to be encoded a 344 - aa protein with the molecular weight of 36kda
應用antheprot5 . 0 、 clustalw等分子生物學軟體分析,顯示主要外膜蛋白前24個氨基酸是較強的疏水性區域,可組成信號肽,其與omp基因的同源率達96 ,氨基酸的同源率高達98 。分享友人