病毒細胞學 的英文怎麼說
中文拼音 [bìngdúxìbāoxué]
病毒細胞學
英文
virocytology- 病 : Ⅰ名詞1 (疾病; 失去健康的狀態) illness; sickness; disease; malum; nosema; malady; morbus; vitium...
- 毒 : Ⅰ名詞1 (對生物體有害的性質或物質; 毒物) poison; toxin 2 (毒品) drug; narcotics 3 (姓氏) a s...
- 細 : 形容詞1 (條狀物橫剖面小) thin; slender 2 (顆粒小) in small particles; fine 3 (音量小) thin ...
- 胞 : Ⅰ名詞1 (胞衣) afterbirth2 (同一個國家或民族的人) fellow countryman; compatriot Ⅱ形容詞(同胞...
- 學 : Ⅰ動詞1 (學習) study; learn 2 (模仿) imitate; mimic Ⅱ名詞1 (學問) learning; knowledge 2 (學...
- 病毒 : [醫學] virus; inframicrobe (濾過性)
- 細胞 : cell; sytes; bioplast; cella; [口語] gene; [生物學] cellule; cellule cellulli cellulo ; cello ; k...
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It is an important that bacteria contaminated vaccine in the biologicals production. we collected 703 samples of cell culture, virus cultivation and harvest which were contaminated by bacteria during poliovaccine production within two years. we checked these samples by bacteriological method and antibiotics sensitivity tests were done. it shows that 1 ) the main contaminated bacteria come from staphylococci, bacilli and streptococci of environment in the poliovaccine production. 2 ) it is effect that antibiotics to contaminated bacteria are doxycycline, albiotic, prescription 2, cefotaxime na salt, gentamycin, neomycin, aureomycin and erythromycin
在疫苗生產實踐中,細菌污染是影響疫苗質量和產量的關鍵性因素,筆者通過了兩年左右的時間,選取正常生產中零星細菌污染的細胞培養瓶、病毒培養瓶及收毒污染樣品等共703份,進行細菌學檢查,並對造成污染的主要細菌種類進行了各種抗菌藥物的耐藥性實驗,結果表明:我所脊灰疫苗生產中主要的污染威脅來自環境中的葡萄球菌,潛在威脅是桿菌和鏈球菌;強力黴素、林可黴素、配方2 、噻孢黴素鈉鹽、慶大黴素、新黴素、金黴素和紅黴素等抗生素對目前引起污染優勢細菌-葡萄球菌有明顯的抑菌效果,可作為疫苗生產后備抗菌手段參考In trpsin tolerance assay. this virus could resist to 1 % trpsis at 37 in an hour. in acid tolerance assay, this virus was resistant to ph3. 0 and ph5. 0 at 37 in 2 hours, and the average infection litre of the virus decreased little. in heat assay, at 50, the virus was processed from 5 minutes to 150 minutes and at each condition the viral virulence reduced to some certain degree. among these conditions, when at 50 in 30 minutes. the average infection litre of this virus decreased over 2 tilre. and when al 50 in an hour, cpe of ihis virus disappeared. when time was set for an hour. but with processed in different temperature as 50 60 70, 80, the virus losl the multiplication capacity complelely. in biological assay, we selected different cell lines to cultivate this virus by laking advantage of possesional cells at that time in our laboratory. then we found that fcwf cell line was the most sensitive to dxmv and mdck was the second. with f81 cell line, after passaged for 12 times continuously with low concentration of fcs. the virus could produce cpe. however, with vero cell line. the virus could not procuce any cpe after many passages. the hemagglutination and lumadsorption reaction test proved that this virus had no any reaction to erythrocyte of pig, fowl and cavy. by neutrolizaion assay, dxmv could be identified as a kind of ccv
理化學研究表明,該病毒為rna病毒,對氯仿、乙醚敏感;胰酶試驗中,經37 、 1小時處理的病毒,仍然能夠在貓源細胞fcwf細胞上生長,並且毒力基本保持不變;耐酸性試驗中,病毒分別在ph5 . 0和ph3 . 0經37作用2小時,毒力僅下降一個滴度;耐熱性試驗中,該病毒在恆定溫度50 ,設定不同時間,從5分鐘到150分鐘,毒力均有不同程度下降,其中, 50作用30分鐘,病毒平均滴度下降2個單位; 50 , 60分鐘, cpe消失;恆定時間1小時,設定不同溫度( 50 - 60 - 70 - 80 ) ,病毒在細胞上完全喪失增殖能力, cpe消失。生物學試驗,利用實驗室現有條件,選擇不同的細胞系對該病毒進行培養,發現該病毒對貓源細胞fcwf最敏感; mdck細胞次之; f81細胞經多次傳代,亦可出現cpe ;而vero細胞則不敏感。血凝試驗表明,該病毒對豬、雞、人及豚鼠的紅細胞均無血凝性。Nayak et al. (1965) studied the immunocytologic and histopatholgic development of experimental swine influenza virus infection in pigs.
Nayak等(1965)研究了豬的實驗性流感病毒感染的免疫細胞學和病理組織學發展。The results of biological tests have demonstrated that allantoic fluid of the first passage virus did n ' t produce macroscopic pathogenic role to chicken embryos and after passaged for four times, gross lesions were observed in chicken embryo. the virus showed typical coronavirus under electron - microscope and it could n ' t form plaque in cef cells and could hemagglutinates chicken red blood cells after treatment with 1 % trypsin. to surprise, the virus replicated in cef cells also showed hemagglutination activity to chicken red blood cells. in addition, the spf chickens which inoculated with the virus isolated from the chicken damaged tissue showed clinical sign and grow lesion, but it ' s gross lesion did n ' t resemble to those of field outbreaks
生物學特性:雞胚尿囊液經離心、磷鎢酸負染后,電鏡觀察該病毒為典型的冠狀病毒;該毒株的第一代尿囊液對雞胚無肉眼可見的致病作用,當繼代到第5代后,胚體嚴重病變;病毒在雞胚中隨著接種時間的延長,其效價增高, 96h可達到48h的2倍;該毒株可在cef上生長,但不能形成明顯的蝕斑;經1胰酶處理后可凝集雞紅細胞;雞胚的第四代尿囊液病毒回歸動物體,病死雞腎臟呈典型的花斑腎,腺胃則未見肉眼可見的病變。Since the important roles of eo protein in the viral infection. immunity and virus - host interaction. the homology of 21 csfv strains was investigated by sequence analysis of eo genes in this study, which will provide some evidence for epidemiological study. in addition, the eo gene of hog cholera lapinized vaccine ( hclv ) strain was expressed in the prokaryotic and eucaryotic systems, and the recombinant proteins were preliminarily analyzed by immunological method
鑒于eo蛋白在病毒感染,誘導機體免疫及與宿主細胞相互關系中的作用,本研究克隆了2株豬瘟病毒eo基因並將其與其它毒株eo基因進行了序列分析,揭示了我國豬瘟病毒流行株之間的遺傳演化關系,為豬瘟病毒的流行病學研究提供依據。Animal health analysis methods - detection of antibodies against classical swine fever by virus neutralisation test and immunochemistry on cell cultures if or ip
動物健康分析方法.病毒中性試驗和細胞培養免疫化學檢測典型豬瘟的抗體From different points of view of the epidemiology, cytogenetics, molecular biology, experimental pathology and preventive medicine, this study revealed and proved that the genotoxicity and carcinogenesis existed in the drinking pond water in the high incidence area of live cancer in guangxi, indicated the feasible preventive and interruptive methods, too
從分析流行病學,細胞遺傳毒理學、分子生物學、實驗病理學和預防醫學的不同角度,揭示和論證了廣西某肝癌高發區居民飲用塘水的基因毒性和致癌性,提出了切實可行的預防和阻斷措施。The tlvl offers a wide range of diagnostic services including necropsy, bacteriology, biochemistry, cytology, haematology, histopathology, parasitology, serology and virology. serological and virological testing for avian influenza h5 surveillance and testing for chemical residues in livestock is also conducted
其中包括解剖、細菌化驗、生物化學化驗、細胞化驗、血液化驗、組織病理化驗、寄生蟲化驗、血清化驗及病毒化驗,並提供監察禽流感的血清及病毒化驗與及禽畜" viruses, plasmids, and transposable genetic elements. " chap 6. in molecular biology of the cell. pp. 273 - 8
細胞內的分子生物學第六章病毒、質體,與轉位基因元件。第273到278頁。It is prescribed mainly for genital herpes, but chemical relatives have value against other herpesvirus infections, such as shingles caused by varicella zoster and inflammation of the retina caused by cytomegalovirus
其處方主要針對生殖器皰疹,但類似的化學藥物對于其他皰疹病毒的感染,像是水痘帶狀皰疹病毒引起的帶狀皰疹,以及巨細胞病毒引起的視網膜發炎等,都相當有潛力。Antibacteral peptide ( abp ) existing in the biosphere with the biotic activity is a kind of little peptide and has the action of antibacteria commonly and even can resist some protozoa, virus and tumour cells. the antibacteral peptide has become one of the hot spots of foreign and domestic scholars " investigavitions who gave more and more favors to it as its no ill effect
抗菌肽( antibacteralpeptides , abp ) ,是生物界中廣泛存在的一類生物活性小肽,一般具有抗細菌或真菌作用,有些還具有抗原蟲、病毒或腫瘤細胞的功能,且無毒副作用,倍受國內外學者的青睞,已成為國內外學者的研究熱點。The reasults are summed up as following : 1 the study on chromosomes and mitoses of bmn cells the cell line, bmn, is a silkworm cell line widely used in silkworm molecular genetics, cell engineering, gene engineering and baculovirus expression system but whose genetics and cytobiology studies are nearly untouched. the chromosomes and mitoses of the bmn cells are researched by the air - drying method and culturing cells on cover glasses
同時,還通過原代培養實驗對新的家蠶胚胎細胞系的建立進行了探索和嘗試,並對家蠶胚胎原代培養過程中出現的細胞和組織類型進行了觀察、探討與研究。 1bmn細胞有絲分裂及染色體研究bmn細胞是家蠶分子遺傳學,細胞工程、基因工程和桿狀病毒表達系統中廣泛應用的家蠶細胞,但其遺傳學和細胞生物學背景知之甚少。Furtherly expression phase analysis showed that orf2 appeared in different form during infection. to reveal the function of haorf2, vha - orf2 - gfp recombinant virus which tagged with egfp - orf2 fusion protein was generated
構建了ha2與綠色熒光蛋白融合表達的重組病毒vha - orf2 - gfp ,對該重組病毒感染細胞后,融合篡碩士學位論文mast卜r 』 s 』 r拼ifsResearchers from the european molecular biology laboratory [ embl ] in grenoble and heidelberg, the institut de biologie structurale [ ibs ] and the unit of irus host cell interactions [ uhci ] *, both in grenoble, hae now produced the first 3 - dimensional image of part of this key protein
現在,格勒諾布爾和海德堡的歐洲分子細胞學實驗室和格勒諾布爾的生物結構研究所及病毒宿主細胞相互作用部門的研究者們展示了部分這種關鍵蛋白質的首個三維影象To find out how the polymerase and importin interact, stephen cusack, head of embl grenoble, and collaborators at the uhci, used the high intensity x - ray source of the european synchrotron radiation facility to generate a high - resolution image of the two proteins interacting with each other
為了揭示多聚酶和輸入蛋白是如何相互作用的格勒諾布爾歐洲細胞生物學實驗室主任及病毒宿主細胞相互作用部門的合作者斯蒂芬.庫薩克用歐洲同步加速器放射中心的高強度x線放射源產生了這兩種蛋白質相互作用的高解析度影象This paper expounds the progresses of the researches on the life science and relevant subjects, which include the research of cultivating the arterial vessel with fine cell tissue, the research on the genetically modified animal, the research on the pest control with bio - virus pesticide, the research on the death mechanism of protein and the diseases of immune system ( including cancer ), the research on anti - senile protein and the research on compound - type aids vaccine
闡述了生命科學及其相關學科的研究進展,包括用細小細胞組織培製成動脈血管的研究、轉基因動物的研究、生物病毒農藥防治蟲害的研究、蛋白質死亡機理及免疫系統疾病(包括癌癥)的研究、抗衰老蛋白的研究以及復合型艾滋病疫苗的研究。Twenty years later scientists found that a virus that causes the veterinary disorder newcastle disease shows a preference for infecting tumor cells and began to try to enhance that tendency by growing the viruses for generations in human cancer cells in laboratory culture dishes
20年後,科學家發現引起新城雞瘟的病毒,特別偏愛感染癌細胞,因此他們在實驗室里的人類癌細胞株中培養此種病毒,試圖加強這種傾向。Nayak et al. ( 1965 ) studied the immunocytologic and histopatholgic development of experimental swine influenza virus infection in pigs
Nayak等( 1965 )研究了豬的實驗性流感病毒感染的免疫細胞學和病理組織學發展。Rapid responses occured when cells were exposed to genotoxic stresses : one is initiating dna damage checkpoint that leads to arrest in gl, in order to provide the cell with sufficient time to repair damaged dna, or leads to arrest in g2, in order to avoid defective chromosome happening mitosis. on the other hand, if damage to the genome is excessive, the cells initiating apoptosis, when damages cannot be repaired
這是因為細胞在受到諸如電離輻射等基因毒應激損傷時,細胞最先作出的細胞學反應:一,起始dnadamagecheckpoint使細胞周期阻滯( arrest )在g1期,贏得時間識別與修復損傷,避免損傷的dna進行錯誤的復制,或和阻滯在g2期,避免缺陷的染色體進一步分裂;二,損傷嚴重而修復無望啟動細胞死亡程序,清除那些帶有病變傾向的細胞危害機體。Hku s state key laboratory of emerging infectious diseases, located at its faculty of medicine, embodies the p3 laboratory and the virus, cell and molecular biology laboratory
香港大學的新發傳染性疾病國家重點實驗室位於醫學院,實驗室包括生物安全三級實驗室( p3laboratory )和病毒、細胞和分子生物學實驗室。分享友人