癌基因檢測 的英文怎麼說
中文拼音 [áijīyīnjiǎncè]
癌基因檢測
英文
oncogene detection- 癌 : 名詞[醫學] (惡性腫瘤) cancer; carcinoma
- 因 : Ⅰ動詞[書面語] (沿襲) follow; carry on Ⅱ介詞1 [書面語] (憑借; 根據) on the basis of; in accord...
- 檢 : Ⅰ動詞1 (查) check up; inspect; examine 2 (約束; 檢點) restrain oneself; be careful in one s c...
- 測 : 動詞1. (測量) survey; fathom; measure 2. (測度; 推測) conjecture; infer
- 檢測 : check; detection; test; gauging; detecting; sensing; [工業] checkout; measuring
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First, to construct a recombinant plasmid pegfp - c - fos with c - fos promoter and egfp, and then transfect it into human bladder transitional cell carcinoma biu - 87 cell ; second, based on the changes of the expression of gfp in the biu - 87 cell which induced by the aconitine and hab toxins, the concentration of the hab toxins could be detected
目的:構建一個含c - fos啟動子和egfp報告基因的pegfp - c - fos重組質粒載體。體外轉染膀胱癌biu - 87細胞后,利用赤潮毒素作用后細胞表達綠色熒光蛋白的變化來檢測赤潮毒素,初步建立一種以細胞為基礎受體水平的赤潮毒素檢測方法。Another promising new test identifies genetic markers for cancer in stool samples
在糞便樣本中的另一種有前景的新檢測手段可鑒別癌基因標志物。Since evidence shows anti - estrogen drugs will fail in a many patients with estrogen - receptor - positive breast cancer, says knudsen, our research suggests that physicians should examine both estrogen receptor status and rb tumor suppressor status during the initial diagnosis, in order to prescribe the most effective therapy for that specific patient ' s cancer.
證據表明,抗雌激素藥物對雌激素受體陽性乳癌將失效,我們的研究提示,為了給那些特殊癌癥患者制定最有效的治療方法,醫生應該在開始診斷時檢測雌激素受體和rb抑癌基因狀況。Since eidence shows anti - estrogen drugs will fail in a many patients with estrogen - receptor - positie breast cancer, says knudsen, our research suggests that physicians should examine both estrogen receptor status and rb tumor suppressor status during the initial diagnosis, in order to prescribe the most effectie therapy for that specific patient ' s cancer.
證據表明,抗雌激素* *物對雌激素受體陽性乳癌將失效,我們的研究提示,為了給那些特殊癌癥患者制定最有效的治療方法,醫生應該在開始診斷時檢測雌激素受體和rb抑癌基因狀況。In our laboratory, a unique mutation detection system using a shuttle vector plasmid has been established to demonstrate that a low concentration of mnng ( 0. 2 m ) can induce nontargeted mutation in mammalian cells : the mammalian cells were exposed to 0. 2m mnng for 2. 5h, then a shuttle plasmid pz189 carrying supf trna gene was transfected into cells after 24h culture. we found a 5 - fold higher mutation frequency of the plasmid replicated in pretreated cells than the spontaneous mutation frequency of the plasmid replicated in control cells. this kind of mutation did not occur immediately after mnng exposure
我們實驗室曾用一特殊的突變檢測系統,直接證明dna損傷劑可在哺乳動物細胞誘發非定標性突變:首先用低濃度( 0 . 2 m )的短壽烷化劑mnng (半壽期為1 . 1hr )處理細胞2 . 5h后,繼續培養24h ,將重組有用作突變檢測的靶基因supftrna基因的穿梭質粒pz189轉入細胞復制,發現在未受致癌物直接攻擊的穿梭質粒中有較自發突變率高5倍以上的靶基因突變。Methods : 1 ) 12h after irradiation, the cell cycle of nih3t3 cells was determined by flow of cytometry and the ratio of alternations in p16 gene exon - 2 was evaluated through pcr - sscp. 2 ) the content of mda, the activities of the sod and gsh - px in the supernatant of nih3t3 cells and the cells were measured by detecting kits immediately after irradiation. 3 ) the level of matrix metalloproteinase - 2 ( mmp - 2 ) in hela cells was detected by western - blotting and dot - blotting 2h after irradiation
具體方法為: ( 1 )照射后12h ,收集nih3t3細胞,用流式細胞儀檢測各組細胞的細胞周期, pcr - sscp檢測抑癌基因p16的變化; ( 2 ) nih3t3細胞照射后立即收集細胞和細胞上清,用試劑盒測量mda含量和sod 、 gsh - px的活性並觀察其變化; ( 3 ) western免疫印跡和點雜交法檢測照射2h后的各組hela細胞中基質金屬蛋白酶- 2 ( mmp - 2 )的表達變化。Chen, j. j. w., peck, k., hong, t. m., yang, s. c., sher, y. p., shih, j. y., wu, r., roffler, s. r., wu, c. w. and yang, p. c
現階段工作在於研發新的生物晶片技術以及應用晶片技術于基因體蛋白體及癌癥和傳染性疾病的基礎研究與檢測應用。Methods dna chip was used to detect the mrna from 11 human transitional cell carcinoma tissues and to investigate the genes related to signal transduction
方法使用人腫瘤基因表達譜晶元檢測11例膀胱移行細胞癌組織基因表達譜的變化,以尋找與細胞信號轉導相關的差異表達基因。In this construct, hf2 dna should be expressed under the control of the camv 35s promoter. the construct was transformed to petunia hybrida of the light pink via agrobacterium tumefaciens eh a105 using the leaf disc method. in the end, three transgenetic plants were obtained by screening with the phosphinothricin resistance and pcr amplification
通過三親交配將重組質粒pc3301 - hf2導入根癌農桿菌eha105 ,抗性篩選、 pcr檢測及dna點雜交表明轉化后的農桿菌帶有完整目的基因片段,能夠用於轉化植物。Ih ped one, a nove1 mo1ecuar probe onolecuar beason ( mb ) was developed to establish a stwle and precise method for the quanitative analysis of gentic materials such as ing1 inrn from differen cells and rna fonn tobacco mosaic virus. the milization method for mb was studied for the fabrication of dna biosensor and dna chip. ih pall two, a series of inunobilization method such as sol - gel method and ligh polererization method were investigaed for the otilization of bio - molecues and fluorescence dye
本論文各章內容可歸納如下:第一章,根據抑癌基因ing1基因的序列設計併合成了檢測ing1轉錄產物的分子信標( 5 ' - - tamra - c6 - nh - cgttgatggttccacttctcgtgcgttcaacg - dabcyl - 3 ' ) ,其中tamra代表四甲基羅丹明,為熒光基團, dabcyl代表4 - (對二甲氨基偶氮苯)苯甲酸,為熒光熄滅基團。In the u. s. alone, four million babies are screened annually for genetic diseases, cancer predisposition, and paternity
在美國,每年就有400萬名嬰兒作基因檢測,檢查遺傳疾病、癌癥傾向和親緣關系。The examination and post - operation quality of life correlation study of apoptosis - suppressing oncoprotein in patients with laryngeal carcinoma
喉癌組織中抗凋亡基因檢測與患者術後生活質量相關性研究Differential gene expression profile in cervical cancer infected with human papillomavirus 18 screened out by cdna microarray
用基因晶元技術檢測宮頸癌患者相關基因的差異表達Multiple kinase assay was performed to examine pkc ^ mapk. tpk activity in the transfected cells. meantime, pegfp - sh2a vector was also constructed and the cells transfected with it were examined by fluorescent microscopy. the expression of sh2a gene was examined under different concentration and time of bfgf as a stimulating factor
1 sh ,利用脂質體轉染肝癌bel7402細胞人os7細胞,檢測pkc 、 mapk 、 tpk活性的改變;流式細胞儀檢測細胞增殖;另構建pegfp sh ,轉染細胞,熒光顯微鏡觀察熒光定位; bfgf作為刺激因素處理細胞,根據不同濃度、時間檢測sh基因表達情況。Other research is looking at multiple genes inoled in colon cancer and at improing screening techniques so more cancers can be detected early
另一些研究則在尋找結腸癌涉及的多種基因,和改進檢測技術以使更多的癌癥得到早期診斷。We reviewed the advances of detective methods of new immunology and gene on gastric carcinoma metastasis, and discussed the combinative application of multi - markers detection
綜述了有關胃癌浸潤轉移的免疫分子和基因的研究現狀,提出了新的免疫學診斷指標在預測腫瘤轉移方面的應用,以及多項指標聯合檢測的可能性。Based on the plant expression vector of pcambia1300 - hbmp - 3m and pcambial 301 - hbmp - 3 constructed, we set up the system of high frequency regeneration and transformation of tomato cotyledons. the hbmp - 3m gene and hbmp - 3 gene were respectively transformed into tomato and tobacco by transfection of agrobacterium tumefaciens. and the transgentic plants were detected. the main results are as follows : l. the plasmid pcambial300 - hbmp - 3m carried hbmp - 3m gene and pcambia - 1301 - hbmp - 3 carried hbmp - 3 gene were respectively transferred into agrobacterium tumefaciens by electroporation
本試驗是在已經構建好的植物表達載體pcambia1300 - hbmp - 3m和pcambia1301 - hbmp - 3載體的基礎上,建立番茄子葉的高頻率再生體系和番茄子葉的高頻遺傳轉化系統,利用根癌農桿菌介導的方法,將人骨形成蛋白- 3成熟肽( hbmp - 3m )基因和人骨形成蛋白- 3 ( hbmp - 3 )全長基因分別導入番茄和煙草,並對轉基因植株進行了檢測。分享友人